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The map label for this gene is lysS [H]

Identifier: 116620161

GI number: 116620161

Start: 1279579

End: 1281084

Strand: Reverse

Name: lysS [H]

Synonym: Acid_1034

Alternate gene names: 116620161

Gene position: 1281084-1279579 (Counterclockwise)

Preceding gene: 116620163

Following gene: 116620160

Centisome position: 12.86

GC content: 61.69

Gene sequence:

>1506_bases
TTGTCCCTGGAAGACGACTTGCTCAAACAGCGTTTGACGCGCATTCGCGAAATCGAAGCCCTCGGCTACCGGGGCTATGG
ACAGCGGTTCGAGTTTACCCATGCCGTGCCCGAGATACTCGCCGAGTATGGAGCCAAAACGGCGGAGGAGCTGGCGCCGG
AGGTTCGGGTCCGCGTTGCCGGACGCCTGATGACGCTGCGCCACATGGGCAAGGCGGGCTTTGCGCACCTGCAGCAGAAT
GGCGAGCGACTGCAGATCTACGTGAAGAAAGACGCAGTGGGCGAACGCGATTACGCGCTGTTCAAGCTGATCGACCTGGG
CGATATCGTCGGCGTGGAGGGATACTTGTTCCGCACGCGCACCGGGGAGCTGAGCATTCACGCGGAGAAGCTGGAATTCC
TGAGCAAGACGCTGCTCTCCATGCCCGAGAAGTGGCATGGCCTGGAAGACGTGGAGACGCGCTACCGGCAGCGCTACCTG
GACCTGATCGCCAACGCCGACGTGCGCAAAGTTTTCGTGACGCGGGCCAAGATCATTTCGTCCCTGCGACGTCAGCTGGA
GGCGCGCAACTTCATCGAAGTGGAGACGCCGATGATGCAGCCGCTTTACGGCGGGGCAGCGGCGCGGCCCTTCGTCACGC
ATCACAATACGCTGGATATCGACCTCTACCTGCGGATCGCGCCCGAGCTCTACCTGAAGCGCTTGGTGGTGGGCGGAATG
GAGCGCGTTTACGAAATCAATCGCAACTTCCGCAACGAAGGGCTATCCACGCATCACAACCCCGAGTTCACGATGTTGGA
GTTCTACCAGGCGTACACGGACTATCGCGGGCTAATCGATTTTTCCGAGGAGCTGCTGCGGCAGGCGGCGATCGACGCCA
CGGGGTCGCCGGTGGTGGAGTTCGAAGGCCATCAGATCGATTTCAGCTGCCTGGCGCGGTACAGCATGCGCGAAGCGATT
GTGCGGCACTGGGAAGGTGACGGTGCGCCGACGATGGAAGACGTGGCCAGCCCCGAATGGCTGAGCGTGCATTCCGGCAA
GGCGACGGCGGGCGAGCGTCTGACAGATATTTTCGAGCGCCATGTGGAAGACAAGTTGATTCAGCCGACGGTGATTTACG
ACTACCCGGTGGAGACTTCGCCGCTTTCGAAGAACAAGCCCGAGGATCCGGCGTTCGTGGAGCGCTTCGAGATCTATGCG
GCGGGCATGGAGATCGGCAACGCATATACCGAGTTGAACGATCCGCAGGAGCAGCGGCGGCGCTTCGAGATGCAGATCGG
CATGCGCGAGCGCGGCGACGAAGAGGCGCACCAGATGGATGAGGACTATGTCCGCGCGCTGGCATACGGGATGCCTCCGA
CCGGAGGCGAAGGGATCGGTATCGACCGGTTGACGATGATCCTGACCGGATCGCGCTCCATCCGCGACGTGATTCTGTTC
CCGCTGCTGCGGCCGGAAGGGCCGATCGAACTGGTGGGGCAACTGCGCGAATCCGACAAACTGTGA

Upstream 100 bases:

>100_bases
GCGGGCAATCGTTTCTCTTCGTTGTACGCAGGAATGATAATGGAAATGGATCGGTTCAAGCGAAGCTTTATAATAGCTTG
TTTCCGGTTAGGAGTTCTAT

Downstream 100 bases:

>100_bases
AGACCTTCGAGCTGTTCATCGCCGGCCGGTATCTGCGAGCGCGCCGCAAGGAGGCGGTGATCTCTGTGATCACGGCGATC
TCGGTGCTCGGCGTGGCGGC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MSLEDDLLKQRLTRIREIEALGYRGYGQRFEFTHAVPEILAEYGAKTAEELAPEVRVRVAGRLMTLRHMGKAGFAHLQQN
GERLQIYVKKDAVGERDYALFKLIDLGDIVGVEGYLFRTRTGELSIHAEKLEFLSKTLLSMPEKWHGLEDVETRYRQRYL
DLIANADVRKVFVTRAKIISSLRRQLEARNFIEVETPMMQPLYGGAAARPFVTHHNTLDIDLYLRIAPELYLKRLVVGGM
ERVYEINRNFRNEGLSTHHNPEFTMLEFYQAYTDYRGLIDFSEELLRQAAIDATGSPVVEFEGHQIDFSCLARYSMREAI
VRHWEGDGAPTMEDVASPEWLSVHSGKATAGERLTDIFERHVEDKLIQPTVIYDYPVETSPLSKNKPEDPAFVERFEIYA
AGMEIGNAYTELNDPQEQRRRFEMQIGMRERGDEEAHQMDEDYVRALAYGMPPTGGEGIGIDRLTMILTGSRSIRDVILF
PLLRPEGPIELVGQLRESDKL

Sequences:

>Translated_501_residues
MSLEDDLLKQRLTRIREIEALGYRGYGQRFEFTHAVPEILAEYGAKTAEELAPEVRVRVAGRLMTLRHMGKAGFAHLQQN
GERLQIYVKKDAVGERDYALFKLIDLGDIVGVEGYLFRTRTGELSIHAEKLEFLSKTLLSMPEKWHGLEDVETRYRQRYL
DLIANADVRKVFVTRAKIISSLRRQLEARNFIEVETPMMQPLYGGAAARPFVTHHNTLDIDLYLRIAPELYLKRLVVGGM
ERVYEINRNFRNEGLSTHHNPEFTMLEFYQAYTDYRGLIDFSEELLRQAAIDATGSPVVEFEGHQIDFSCLARYSMREAI
VRHWEGDGAPTMEDVASPEWLSVHSGKATAGERLTDIFERHVEDKLIQPTVIYDYPVETSPLSKNKPEDPAFVERFEIYA
AGMEIGNAYTELNDPQEQRRRFEMQIGMRERGDEEAHQMDEDYVRALAYGMPPTGGEGIGIDRLTMILTGSRSIRDVILF
PLLRPEGPIELVGQLRESDKL
>Mature_500_residues
SLEDDLLKQRLTRIREIEALGYRGYGQRFEFTHAVPEILAEYGAKTAEELAPEVRVRVAGRLMTLRHMGKAGFAHLQQNG
ERLQIYVKKDAVGERDYALFKLIDLGDIVGVEGYLFRTRTGELSIHAEKLEFLSKTLLSMPEKWHGLEDVETRYRQRYLD
LIANADVRKVFVTRAKIISSLRRQLEARNFIEVETPMMQPLYGGAAARPFVTHHNTLDIDLYLRIAPELYLKRLVVGGME
RVYEINRNFRNEGLSTHHNPEFTMLEFYQAYTDYRGLIDFSEELLRQAAIDATGSPVVEFEGHQIDFSCLARYSMREAIV
RHWEGDGAPTMEDVASPEWLSVHSGKATAGERLTDIFERHVEDKLIQPTVIYDYPVETSPLSKNKPEDPAFVERFEIYAA
GMEIGNAYTELNDPQEQRRRFEMQIGMRERGDEEAHQMDEDYVRALAYGMPPTGGEGIGIDRLTMILTGSRSIRDVILFP
LLRPEGPIELVGQLRESDKL

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=509, Percent_Identity=39.2927308447937, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI194272210, Length=509, Percent_Identity=39.2927308447937, Blast_Score=336, Evalue=4e-92,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=48.9795918367347, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=49.7959183673469, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=31.0769230769231, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788173, Length=182, Percent_Identity=27.4725274725275, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=503, Percent_Identity=40.9542743538767, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17535927, Length=499, Percent_Identity=41.0821643286573, Blast_Score=353, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71994340, Length=499, Percent_Identity=41.0821643286573, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17551876, Length=475, Percent_Identity=23.3684210526316, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=254, Percent_Identity=24.8031496062992, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=43.4442270058708, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=486, Percent_Identity=32.0987654320988, Blast_Score=200, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24640849, Length=484, Percent_Identity=42.5619834710744, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24640851, Length=484, Percent_Identity=42.5619834710744, Blast_Score=353, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57495; Mature: 57364

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEDDLLKQRLTRIREIEALGYRGYGQRFEFTHAVPEILAEYGAKTAEELAPEVRVRVA
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCHHHHHHHH
GRLMTLRHMGKAGFAHLQQNGERLQIYVKKDAVGERDYALFKLIDLGDIVGVEGYLFRTR
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHEEEHHHHHCCCCCEEEEEE
TGELSIHAEKLEFLSKTLLSMPEKWHGLEDVETRYRQRYLDLIANADVRKVFVTRAKIIS
CCCEEEEHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SLRRQLEARNFIEVETPMMQPLYGGAAARPFVTHHNTLDIDLYLRIAPELYLKRLVVGGM
HHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEECCCEEEEEEEEEECHHHHHHHHHHHHH
ERVYEINRNFRNEGLSTHHNPEFTMLEFYQAYTDYRGLIDFSEELLRQAAIDATGSPVVE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FEGHQIDFSCLARYSMREAIVRHWEGDGAPTMEDVASPEWLSVHSGKATAGERLTDIFER
ECCCEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHH
HVEDKLIQPTVIYDYPVETSPLSKNKPEDPAFVERFEIYAAGMEIGNAYTELNDPQEQRR
HHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHH
RFEMQIGMRERGDEEAHQMDEDYVRALAYGMPPTGGEGIGIDRLTMILTGSRSIRDVILF
HHHHHCCCHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCHHHHHHHH
PLLRPEGPIELVGQLRESDKL
HCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLEDDLLKQRLTRIREIEALGYRGYGQRFEFTHAVPEILAEYGAKTAEELAPEVRVRVA
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCHHHHHHHH
GRLMTLRHMGKAGFAHLQQNGERLQIYVKKDAVGERDYALFKLIDLGDIVGVEGYLFRTR
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHEEEHHHHHCCCCCEEEEEE
TGELSIHAEKLEFLSKTLLSMPEKWHGLEDVETRYRQRYLDLIANADVRKVFVTRAKIIS
CCCEEEEHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SLRRQLEARNFIEVETPMMQPLYGGAAARPFVTHHNTLDIDLYLRIAPELYLKRLVVGGM
HHHHHHHHCCEEEECCCHHHHHCCCCCCCCEEECCCEEEEEEEEEECHHHHHHHHHHHHH
ERVYEINRNFRNEGLSTHHNPEFTMLEFYQAYTDYRGLIDFSEELLRQAAIDATGSPVVE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FEGHQIDFSCLARYSMREAIVRHWEGDGAPTMEDVASPEWLSVHSGKATAGERLTDIFER
ECCCEECHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCCCCCHHHHHHHHHHH
HVEDKLIQPTVIYDYPVETSPLSKNKPEDPAFVERFEIYAAGMEIGNAYTELNDPQEQRR
HHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHH
RFEMQIGMRERGDEEAHQMDEDYVRALAYGMPPTGGEGIGIDRLTMILTGSRSIRDVILF
HHHHHCCCHHCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCCHHHHHHHH
PLLRPEGPIELVGQLRESDKL
HCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10737207 [H]