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The map label for this gene is trxB [C]

Identifier: 116620157

GI number: 116620157

Start: 1274184

End: 1275836

Strand: Reverse

Name: trxB [C]

Synonym: Acid_1030

Alternate gene names: 116620157

Gene position: 1275836-1274184 (Counterclockwise)

Preceding gene: 116620160

Following gene: 116620151

Centisome position: 12.8

GC content: 63.46

Gene sequence:

>1653_bases
ATGTCGATGCTGCCGCCGGAAACCCCGGAACAACACACTTTGCGATTTCCGAAACTGAGCGCCGCGCAAATCGCGCAACT
GGCGCCTTGGTGTGTGCGGCGTCACGCAAATGCCGGAGAGGTGGTCTACGACGTGGGTAGCGCGGCCCATGGCGTCTACA
TTGTGGAGAGCGGGAGCCTGGAAATCATCGGCGTCACCAGCGGGGTGGAGACCGTGGTGGCTGTCCTGGAACCCGGCGAG
TTCACCGGAGAGACCAATCTGCTTTCAGGGCGCCGCGCGCTAGTGAGGTGCCGCGCACGTTCGGCCAGTGCGCTTTTGGA
AATCGAGCGGGCGAACCTGCGGCGCGTGATGCAGAGCGATGCGACCCTCGGCGATTTGTTTCTGCGGGCATTTCTACTAC
GACGCGTTTTCCTGATTGCGAATTCGGTGGGCGACGCGGTTCTGATCGGATCGAAACATTCGGCCGACACGCTGCGCCTG
CGAACATTCCTGGGACGCAATGGACACCCTTACGCGTACCTCGACGTGGATGCGGATGCAGACGTCCAGAACGTGCTGGA
CCATTTCTCGGTGCGAATCGACGAGATCCCGGTGTTGATCTGCCGCGGCGAGCTGGTGCTGCGCAATCCGACCAATGCGG
AGGCGGCGGTCTGTTTCGGACTCAACGCGGGAATCGAAGCCGAGGGTGTGTACGACCTGATCGTGATCGGCGCGGGACCG
GGCGGACTGGCGGCTGCGGTGTACGGGGCATCGGAAGGGCTCTCCACGCTGGTGGTGGAATGCAATGCGCCGGGAGGGCA
GGCGGGGTCCAGTTCGCGGATTGAGAACTACCTGGGTTTTCCGATGGGGATCTCGGGACAGGAACTGGCGGACCGCGCAT
TTCTGCAGGCCGAGAAATTCGGCGCGCAGATTTCGGTGGCACGGACGGCAACGGAGCTCAAGTGCCGCCGGCCTCCCTAC
GCGGTGCAGCTGGACGATGGATCCGTGGTGCAGGGCCGCACCATGATCGTGGCGTCGGGCTCGCGGTATCGCAGGCTGGA
TCTGCCGAACATCGGAAAGTTCGAAGGGTCGGGGATCTACTACGGGGCCACCACCGTGGAGGCGCCGCTGTGCCAAAGCG
AGGTGGTCGCGGTGGTGGGCGGCGGAAACTCCGCGGGTCAGGCGGCGGTGTTCTTGTCAGGATACGCGAGCCACGTACAC
CTGCTGGTTCGCGCGCCGGGGCTCGCGGCCACCATGTCGCGCTATCTGATCGCGCGGATCGAGGCGTCGCCGGAGATTAC
CGTGCACACCTTCACGAACGTGGAAGCATTGGAAGGAGACACGCGGCTGGAGCGCATTCGCTGGCGCAACACAAAGACCG
GGATGACAGAGAGCCATGAGATTCATCATCTCTTTCTGATGATGGGGGCAGACCCCAATACCGGCTGGCTGAGCGAATGT
GTGGCGCTGGATGGCAAGCGATTTATCAAGACCGGTCCGGAGTTGGCCGACGATTGGCCGCTGCAGCGAGCGCCTTTCCC
GCTGGAGACGAGCCTGCCCGGGATCTTCGCGGTGGGCGATATACGGGCGAACAGCATCAAGCGCGTGGCCTCGGCAGTGG
GAGAAGGATCGATGGCGGTGCAGTTCGTCCACCAGGTTCTGGCCGGTACGTGA

Upstream 100 bases:

>100_bases
CATGATCTCCAGTTCCTGCTCGGTCAAGGTGGTGCTTCGCCGTCGCATACTAATTAATTAGTACTAAAAAGAGAAGATGT
CAAGGGGAAAATAAGAACTG

Downstream 100 bases:

>100_bases
GGCTCAGGGCCGGCGCAGCGCGGGGCAGCAATCGGGCGCGCAGAGCGGAGTTTCGGGCGAGGCCTGACGCCGCGCGATCA
GGTCGGCGAGCATCGCGATC

Product: cyclic nucleotide-regulated FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: ORF A [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSMLPPETPEQHTLRFPKLSAAQIAQLAPWCVRRHANAGEVVYDVGSAAHGVYIVESGSLEIIGVTSGVETVVAVLEPGE
FTGETNLLSGRRALVRCRARSASALLEIERANLRRVMQSDATLGDLFLRAFLLRRVFLIANSVGDAVLIGSKHSADTLRL
RTFLGRNGHPYAYLDVDADADVQNVLDHFSVRIDEIPVLICRGELVLRNPTNAEAAVCFGLNAGIEAEGVYDLIVIGAGP
GGLAAAVYGASEGLSTLVVECNAPGGQAGSSSRIENYLGFPMGISGQELADRAFLQAEKFGAQISVARTATELKCRRPPY
AVQLDDGSVVQGRTMIVASGSRYRRLDLPNIGKFEGSGIYYGATTVEAPLCQSEVVAVVGGGNSAGQAAVFLSGYASHVH
LLVRAPGLAATMSRYLIARIEASPEITVHTFTNVEALEGDTRLERIRWRNTKTGMTESHEIHHLFLMMGADPNTGWLSEC
VALDGKRFIKTGPELADDWPLQRAPFPLETSLPGIFAVGDIRANSIKRVASAVGEGSMAVQFVHQVLAGT

Sequences:

>Translated_550_residues
MSMLPPETPEQHTLRFPKLSAAQIAQLAPWCVRRHANAGEVVYDVGSAAHGVYIVESGSLEIIGVTSGVETVVAVLEPGE
FTGETNLLSGRRALVRCRARSASALLEIERANLRRVMQSDATLGDLFLRAFLLRRVFLIANSVGDAVLIGSKHSADTLRL
RTFLGRNGHPYAYLDVDADADVQNVLDHFSVRIDEIPVLICRGELVLRNPTNAEAAVCFGLNAGIEAEGVYDLIVIGAGP
GGLAAAVYGASEGLSTLVVECNAPGGQAGSSSRIENYLGFPMGISGQELADRAFLQAEKFGAQISVARTATELKCRRPPY
AVQLDDGSVVQGRTMIVASGSRYRRLDLPNIGKFEGSGIYYGATTVEAPLCQSEVVAVVGGGNSAGQAAVFLSGYASHVH
LLVRAPGLAATMSRYLIARIEASPEITVHTFTNVEALEGDTRLERIRWRNTKTGMTESHEIHHLFLMMGADPNTGWLSEC
VALDGKRFIKTGPELADDWPLQRAPFPLETSLPGIFAVGDIRANSIKRVASAVGEGSMAVQFVHQVLAGT
>Mature_549_residues
SMLPPETPEQHTLRFPKLSAAQIAQLAPWCVRRHANAGEVVYDVGSAAHGVYIVESGSLEIIGVTSGVETVVAVLEPGEF
TGETNLLSGRRALVRCRARSASALLEIERANLRRVMQSDATLGDLFLRAFLLRRVFLIANSVGDAVLIGSKHSADTLRLR
TFLGRNGHPYAYLDVDADADVQNVLDHFSVRIDEIPVLICRGELVLRNPTNAEAAVCFGLNAGIEAEGVYDLIVIGAGPG
GLAAAVYGASEGLSTLVVECNAPGGQAGSSSRIENYLGFPMGISGQELADRAFLQAEKFGAQISVARTATELKCRRPPYA
VQLDDGSVVQGRTMIVASGSRYRRLDLPNIGKFEGSGIYYGATTVEAPLCQSEVVAVVGGGNSAGQAAVFLSGYASHVHL
LVRAPGLAATMSRYLIARIEASPEITVHTFTNVEALEGDTRLERIRWRNTKTGMTESHEIHHLFLMMGADPNTGWLSECV
ALDGKRFIKTGPELADDWPLQRAPFPLETSLPGIFAVGDIRANSIKRVASAVGEGSMAVQFVHQVLAGT

Specific function: Unknown

COG id: COG0492

COG function: function code O; Thioredoxin reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787114, Length=322, Percent_Identity=30.1242236024845, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081763, Length=340, Percent_Identity=27.9411764705882, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321898, Length=321, Percent_Identity=31.7757009345794, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320560, Length=319, Percent_Identity=31.974921630094, Blast_Score=130, Evalue=5e-31,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 58776; Mature: 58645

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50042 CNMP_BINDING_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLPPETPEQHTLRFPKLSAAQIAQLAPWCVRRHANAGEVVYDVGSAAHGVYIVESGSL
CCCCCCCCCHHHCEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCE
EIIGVTSGVETVVAVLEPGEFTGETNLLSGRRALVRCRARSASALLEIERANLRRVMQSD
EEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHEEEHHHHHHHHHHCC
ATLGDLFLRAFLLRRVFLIANSVGDAVLIGSKHSADTLRLRTFLGRNGHPYAYLDVDADA
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCC
DVQNVLDHFSVRIDEIPVLICRGELVLRNPTNAEAAVCFGLNAGIEAEGVYDLIVIGAGP
CHHHHHHHHHEEECCCCEEEEECCEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEECCC
GGLAAAVYGASEGLSTLVVECNAPGGQAGSSSRIENYLGFPMGISGQELADRAFLQAEKF
CCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
GAQISVARTATELKCRRPPYAVQLDDGSVVQGRTMIVASGSRYRRLDLPNIGKFEGSGIY
CCEEEEEHHHHHHEECCCCEEEEECCCCEECCCEEEEECCCCEEEECCCCCCCCCCCCEE
YGATTVEAPLCQSEVVAVVGGGNSAGQAAVFLSGYASHVHLLVRAPGLAATMSRYLIARI
EECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHEEEE
EASPEITVHTFTNVEALEGDTRLERIRWRNTKTGMTESHEIHHLFLMMGADPNTGWLSEC
CCCCCEEEEEECCCEECCCCHHHHHHEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHH
VALDGKRFIKTGPELADDWPLQRAPFPLETSLPGIFAVGDIRANSIKRVASAVGEGSMAV
HHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHH
QFVHQVLAGT
HHHHHHHCCC
>Mature Secondary Structure 
SMLPPETPEQHTLRFPKLSAAQIAQLAPWCVRRHANAGEVVYDVGSAAHGVYIVESGSL
CCCCCCCCHHHCEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEECCCE
EIIGVTSGVETVVAVLEPGEFTGETNLLSGRRALVRCRARSASALLEIERANLRRVMQSD
EEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCHHHEEEHHHHHHHHHHCC
ATLGDLFLRAFLLRRVFLIANSVGDAVLIGSKHSADTLRLRTFLGRNGHPYAYLDVDADA
CHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCC
DVQNVLDHFSVRIDEIPVLICRGELVLRNPTNAEAAVCFGLNAGIEAEGVYDLIVIGAGP
CHHHHHHHHHEEECCCCEEEEECCEEEECCCCCCEEEEEECCCCCCCCCEEEEEEEECCC
GGLAAAVYGASEGLSTLVVECNAPGGQAGSSSRIENYLGFPMGISGQELADRAFLQAEKF
CCCEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
GAQISVARTATELKCRRPPYAVQLDDGSVVQGRTMIVASGSRYRRLDLPNIGKFEGSGIY
CCEEEEEHHHHHHEECCCCEEEEECCCCEECCCEEEEECCCCEEEECCCCCCCCCCCCEE
YGATTVEAPLCQSEVVAVVGGGNSAGQAAVFLSGYASHVHLLVRAPGLAATMSRYLIARI
EECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEEECCCHHHHHHHHHEEEE
EASPEITVHTFTNVEALEGDTRLERIRWRNTKTGMTESHEIHHLFLMMGADPNTGWLSEC
CCCCCEEEEEECCCEECCCCHHHHHHEECCCCCCCCCCCCEEEEEEEEECCCCCCHHHHH
VALDGKRFIKTGPELADDWPLQRAPFPLETSLPGIFAVGDIRANSIKRVASAVGEGSMAV
HHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHH
QFVHQVLAGT
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1637309 [H]