Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 116619993

GI number: 116619993

Start: 1073992

End: 1075776

Strand: Reverse

Name: gatA [H]

Synonym: Acid_0865

Alternate gene names: 116619993

Gene position: 1075776-1073992 (Counterclockwise)

Preceding gene: 116619994

Following gene: 116619992

Centisome position: 10.79

GC content: 63.7

Gene sequence:

>1785_bases
ATGAAGACCGAGATTCGTCTGGACCTCGCGTCCCTGCGCGAGTTGTACAAGTCCGGCAGAGCCACGCCTTCCGACGTAAT
CAGCGCGATTTACGATCGCCTGGCGCAAGGCCCGCTCGAGCCGGTCTGGATTTCCACTTTCCCGCGCGACAAGGCGATGT
CGAAGGTGCGCAAGTTGGAGCGCGATCCGCTCGCCTCCGCGCTGCCTCTCTACGGCGTGCCGTTTGCGGTCAAGGACAAC
ATCGATGTCGCCGGACTGCCAACTACCGCCGGATGTCCGGCGTACAGCTATTCGCCGGGGCACAGCGCGAGGGTGGTCCA
CTCGCTGATGGAAGCCGGGGCCATCCCGATCGGCAAGACGAATATGGATCAGTTCGCCACAGGGCTGGTGGGGACGCGCT
CGCCACACGGCGCCTGTTCCAGCGTATTCAATTCCCGTTATATTTCCGGTGGGTCCAGTTCCGGGTCCGCGGTAGCCGTG
GCTTCCGGTCTTGCCAGCTTCTCTCTGGGTACCGATACCGCGGGCTCGGGACGCGTGCCCGCGGCGTTCAACGGACTGGT
CGGGCTCAAGCCCACTCGAGGCGTCCTCAGCACTAAGGGGGTGGTGCCCGCGTGCCGCACGCTGGACTGCGTCTCGATTT
TCGCGGAGAATGCCGATGATGCACACACGATCTGGCTCGCCGCGCGAGGCTTCGATGCAGACGATCCTTTTTCCCGCGCA
GCGCGTCCGGGTGAAGATTCGGCGCCGTGGCTCGCGGGGACATTTCGATTCGGCGTGCCGCCCGCGGACCAACTCGAATT
TTTCGGTGATGAAGGGGCCGCGACACTTTATGCCGAAGCGGTCGCGAGACTGGAACGGGTCGGTGGACGCAAAGTGGAGA
TCGACTTTTCCACCTTCCGCGCCGCCGCCGAACTGCTTTACGCGGGCCCCTGGGTGGCGGAGCGGTACGCTGCGATCCGC
GAGTTCATTGAAGCACATGCAAGTGAGATGAATCCAGTAGTCCGTGGAATTATAGAAGGCGCGCGCCGATATTCGGCCGC
CGACGCGTTCGACGCCGAGTACCGCCTGCGCGAATTGCGCCGGGCCACGGAAACCCAGTGGGAGGAGATGGACGTTCTGG
TGCTGCCCACCACGGGCACCATCTACACGCACGATGCGGTTGCTTCCGAACCCGTGCGCATGAACACCAATCTCGGCTAT
TACACGAATTTCGTCAACCTGCTGGACCTGGCGGCGCTCGCGATACCGGCCGGCCGGCGTCCGGACGGCCTCCCGTTTGG
CATCTCTTTAATCGGCCGTGCCTTCAGCGATGAAGCCCTGCTTTCGCTCGCCAAGCGTTTGGAGGGAGAGGGCGCAGCTT
CCGCCGCCCGAACTTGTCCCACTGGCTGCGTCGCGCTCGCGGTGGTCGGCGCGCACCTCTCGGGACAGCCGCTGAATTGG
CAACTTACCGATCGCGGCGCGCGGCTTCTGAAAACCTGCAGGACTTCGGCGGATTATCGCCTGTATGCGCTCGAGGGGAC
AATGCCGCCCAAACCTGGTCTCGTCCGGGAGCGAGATTTCCGCGGCCCGGGCATCGAAGTCGAAGTCTGGGCTATACCGG
AGGACCGGTTCGGCAGCTTCGTAGCCGACGTTCCGGCACCCCTGGGCATCGGCAACGCCATCCTGGATGACGGGCAGACC
GTGAAGTGTTTCATCTGTGAGGCTTACGCCATCGCCGGAGCCGCCGAAGTCACCCGCTTTGGCGGCTGGCGCAACTACCT
TTCGCAGCCGGTATCACAAAGATAG

Upstream 100 bases:

>100_bases
CCGCGCCTAGCGCAGGGGTTATCGAAATGGTGAATTGCATACCCGGAGCGCTGGTCTCGGCGGGCCAGCGATTGGTGACT
TTAAGGCGGGAGATATGACG

Downstream 100 bases:

>100_bases
ATCGGGCTCATAAACAAGATTTTGCGGGGTCCGTGATTCCCCGGTGATAGAATTCGACAATAGCTGCAGGGGCCGCGTCG
CCGCTGACTGATCGCACTTA

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MKTEIRLDLASLRELYKSGRATPSDVISAIYDRLAQGPLEPVWISTFPRDKAMSKVRKLERDPLASALPLYGVPFAVKDN
IDVAGLPTTAGCPAYSYSPGHSARVVHSLMEAGAIPIGKTNMDQFATGLVGTRSPHGACSSVFNSRYISGGSSSGSAVAV
ASGLASFSLGTDTAGSGRVPAAFNGLVGLKPTRGVLSTKGVVPACRTLDCVSIFAENADDAHTIWLAARGFDADDPFSRA
ARPGEDSAPWLAGTFRFGVPPADQLEFFGDEGAATLYAEAVARLERVGGRKVEIDFSTFRAAAELLYAGPWVAERYAAIR
EFIEAHASEMNPVVRGIIEGARRYSAADAFDAEYRLRELRRATETQWEEMDVLVLPTTGTIYTHDAVASEPVRMNTNLGY
YTNFVNLLDLAALAIPAGRRPDGLPFGISLIGRAFSDEALLSLAKRLEGEGAASAARTCPTGCVALAVVGAHLSGQPLNW
QLTDRGARLLKTCRTSADYRLYALEGTMPPKPGLVRERDFRGPGIEVEVWAIPEDRFGSFVADVPAPLGIGNAILDDGQT
VKCFICEAYAIAGAAEVTRFGGWRNYLSQPVSQR

Sequences:

>Translated_594_residues
MKTEIRLDLASLRELYKSGRATPSDVISAIYDRLAQGPLEPVWISTFPRDKAMSKVRKLERDPLASALPLYGVPFAVKDN
IDVAGLPTTAGCPAYSYSPGHSARVVHSLMEAGAIPIGKTNMDQFATGLVGTRSPHGACSSVFNSRYISGGSSSGSAVAV
ASGLASFSLGTDTAGSGRVPAAFNGLVGLKPTRGVLSTKGVVPACRTLDCVSIFAENADDAHTIWLAARGFDADDPFSRA
ARPGEDSAPWLAGTFRFGVPPADQLEFFGDEGAATLYAEAVARLERVGGRKVEIDFSTFRAAAELLYAGPWVAERYAAIR
EFIEAHASEMNPVVRGIIEGARRYSAADAFDAEYRLRELRRATETQWEEMDVLVLPTTGTIYTHDAVASEPVRMNTNLGY
YTNFVNLLDLAALAIPAGRRPDGLPFGISLIGRAFSDEALLSLAKRLEGEGAASAARTCPTGCVALAVVGAHLSGQPLNW
QLTDRGARLLKTCRTSADYRLYALEGTMPPKPGLVRERDFRGPGIEVEVWAIPEDRFGSFVADVPAPLGIGNAILDDGQT
VKCFICEAYAIAGAAEVTRFGGWRNYLSQPVSQR
>Mature_594_residues
MKTEIRLDLASLRELYKSGRATPSDVISAIYDRLAQGPLEPVWISTFPRDKAMSKVRKLERDPLASALPLYGVPFAVKDN
IDVAGLPTTAGCPAYSYSPGHSARVVHSLMEAGAIPIGKTNMDQFATGLVGTRSPHGACSSVFNSRYISGGSSSGSAVAV
ASGLASFSLGTDTAGSGRVPAAFNGLVGLKPTRGVLSTKGVVPACRTLDCVSIFAENADDAHTIWLAARGFDADDPFSRA
ARPGEDSAPWLAGTFRFGVPPADQLEFFGDEGAATLYAEAVARLERVGGRKVEIDFSTFRAAAELLYAGPWVAERYAAIR
EFIEAHASEMNPVVRGIIEGARRYSAADAFDAEYRLRELRRATETQWEEMDVLVLPTTGTIYTHDAVASEPVRMNTNLGY
YTNFVNLLDLAALAIPAGRRPDGLPFGISLIGRAFSDEALLSLAKRLEGEGAASAARTCPTGCVALAVVGAHLSGQPLNW
QLTDRGARLLKTCRTSADYRLYALEGTMPPKPGLVRERDFRGPGIEVEVWAIPEDRFGSFVADVPAPLGIGNAILDDGQT
VKCFICEAYAIAGAAEVTRFGGWRNYLSQPVSQR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=26.4529058116232, Blast_Score=125, Evalue=2e-28,
Organism=Homo sapiens, GI195972892, Length=475, Percent_Identity=22.7368421052632, Blast_Score=94, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17543272, Length=433, Percent_Identity=30.0230946882217, Blast_Score=149, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17537465, Length=416, Percent_Identity=25.4807692307692, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=204, Percent_Identity=29.9019607843137, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=197, Percent_Identity=26.9035532994924, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=186, Percent_Identity=31.1827956989247, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=602, Percent_Identity=44.3521594684385, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6323950, Length=436, Percent_Identity=26.605504587156, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652985, Length=441, Percent_Identity=27.891156462585, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922090, Length=441, Percent_Identity=27.891156462585, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652981, Length=441, Percent_Identity=27.891156462585, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24652983, Length=441, Percent_Identity=27.891156462585, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=26.7068273092369, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356731, Length=477, Percent_Identity=25.9958071278826, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24644968, Length=434, Percent_Identity=23.963133640553, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550774, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648435, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648437, Length=210, Percent_Identity=27.6190476190476, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 63507; Mature: 63507

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTEIRLDLASLRELYKSGRATPSDVISAIYDRLAQGPLEPVWISTFPRDKAMSKVRKLE
CCCEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHH
RDPLASALPLYGVPFAVKDNIDVAGLPTTAGCPAYSYSPGHSARVVHSLMEAGAIPIGKT
CCCCHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
NMDQFATGLVGTRSPHGACSSVFNSRYISGGSSSGSAVAVASGLASFSLGTDTAGSGRVP
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCC
AAFNGLVGLKPTRGVLSTKGVVPACRTLDCVSIFAENADDAHTIWLAARGFDADDPFSRA
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHC
ARPGEDSAPWLAGTFRFGVPPADQLEFFGDEGAATLYAEAVARLERVGGRKVEIDFSTFR
CCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHH
AAAELLYAGPWVAERYAAIREFIEAHASEMNPVVRGIIEGARRYSAADAFDAEYRLRELR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RATETQWEEMDVLVLPTTGTIYTHDAVASEPVRMNTNLGYYTNFVNLLDLAALAIPAGRR
HHHHCHHHHCCEEEECCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCC
PDGLPFGISLIGRAFSDEALLSLAKRLEGEGAASAARTCPTGCVALAVVGAHLSGQPLNW
CCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEE
QLTDRGARLLKTCRTSADYRLYALEGTMPPKPGLVRERDFRGPGIEVEVWAIPEDRFGSF
EECHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCH
VADVPAPLGIGNAILDDGQTVKCFICEAYAIAGAAEVTRFGGWRNYLSQPVSQR
HHHCCCCCCCCCHHHCCCCEEEEEEEEHHHHCCHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MKTEIRLDLASLRELYKSGRATPSDVISAIYDRLAQGPLEPVWISTFPRDKAMSKVRKLE
CCCEEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHH
RDPLASALPLYGVPFAVKDNIDVAGLPTTAGCPAYSYSPGHSARVVHSLMEAGAIPIGKT
CCCCHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
NMDQFATGLVGTRSPHGACSSVFNSRYISGGSSSGSAVAVASGLASFSLGTDTAGSGRVP
CHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCC
AAFNGLVGLKPTRGVLSTKGVVPACRTLDCVSIFAENADDAHTIWLAARGFDADDPFSRA
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHC
ARPGEDSAPWLAGTFRFGVPPADQLEFFGDEGAATLYAEAVARLERVGGRKVEIDFSTFR
CCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHH
AAAELLYAGPWVAERYAAIREFIEAHASEMNPVVRGIIEGARRYSAADAFDAEYRLRELR
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RATETQWEEMDVLVLPTTGTIYTHDAVASEPVRMNTNLGYYTNFVNLLDLAALAIPAGRR
HHHHCHHHHCCEEEECCCCCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCC
PDGLPFGISLIGRAFSDEALLSLAKRLEGEGAASAARTCPTGCVALAVVGAHLSGQPLNW
CCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEE
QLTDRGARLLKTCRTSADYRLYALEGTMPPKPGLVRERDFRGPGIEVEVWAIPEDRFGSF
EECHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCH
VADVPAPLGIGNAILDDGQTVKCFICEAYAIAGAAEVTRFGGWRNYLSQPVSQR
HHHCCCCCCCCCHHHCCCCEEEEEEEEHHHHCCHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA