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The map label for this gene is rpoD [H]

Identifier: 116619924

GI number: 116619924

Start: 992701

End: 994257

Strand: Direct

Name: rpoD [H]

Synonym: Acid_0796

Alternate gene names: 116619924

Gene position: 992701-994257 (Clockwise)

Preceding gene: 116619923

Following gene: 116619925

Centisome position: 9.96

GC content: 59.15

Gene sequence:

>1557_bases
ATGCCTGTGGGGGAGGTGAGTCTTAAAGTGGATCAATTCGAAGATCAGTTTTTAGCGGAAAACCCGGAAGCCCTCGGCCT
CAGTTTTGAAGACGACCCCAAGTTCTTCGATCCGGAGGCTGCGCACGAAGCCGACTTTCTACACCCTGCGCCCGTGGAAG
AGTCCATCTACACGGACGACCCGGTGCGCGTTTACCTTCGTGAAATGGGCGCGGTGCCGCTGCTGACTCGCGAAGGTGAA
GTGGATTTGGCCCGCCGCATGGAGCGTGGGAAGCTGCGGATGCAGAAGGCCATCAGCCGGTCTGCACTGGTACAGCGGGT
AGTTGTCGATGTGGCCGACCAGTTGAAGCGCGCCAACATCGAAATTGAAAGCGTCGTCGATTTGGGAGATGTCGAAGAAG
GCTCCCCGGCGGACGTGAAAGTGCGCAACGAAGCGGCCAAGCATTTTGCCGATATCGTGGTGTTACACAAGAAGTTACAG
CAGCTCGAAGACAAAGTGGAAGCGACTCCGCTGGCCAGCAAGAAGCCGCGCAAGCGGCTCTGCGCCAAGCTCAATCGCTC
CAAAGTAGAGACGTCGCTGGCGATTCGCCGCGCGCCCTTCCGCCTCATTAAGTGGAAGGAATTCGCTCGCGAGATTGAGC
GCGCAGTGGACGAAATCAGCCATCTCGATAGTGAAATCAAGCGTTTAGAGGTGCGGAGCAATCCCACCCAGCAGCTTCGT
CTGCGCGAACTGAAGCGCGAAATCAAGAAACGCGAGATCGCCGCCGGCGCCACCCTGCCGGAACTCAAGCACAGCCTGAC
CGTGATCCGGCACGGTGAAGCCGAAGCGGAGCGCGCCAAGAAGGACCTGGTCGAGGCCAATCTCCGCCTGGTCGTCTCGG
TCGCCAAGAAATACGTGAACCGCGGACTTCACCTGCTGGACTTGATCCAGGAGGGCAACATCGGCCTCATGCGCGCCGCC
GATAAGTTCGAATACCGCCGCGGCTACAAGTTCTCAACCTATGCCACGTGGTGGATCCGCCAGGCGATCACGCGCGCCAT
CGCGGATCAATCGCGCACCATCCGCATCCCTGTTCATATGAACGAGAGTATGAACAAATTCCTGCGGGCGACGCGGGAAT
TGGAAAAGGAACTGGGACGAACTCCTACAAACGACGAAATTGGTCGCCGTATGGATATCCCGGTGGAGAAGGTCCAGAAG
CTCAAGACCATCTCCCGCGACCCTGTCTCTCTCGAAACCCCGGTTGGCCGCGACGGCGAATCGGCGTTAGGCGATCTGAT
CGAAGATCGCTGGGTCGGTTCCCCGGTGGATGCCGTTATCGAATCCAATGTCCGCGACGAAACCGCCGGCATTTTGAAGA
CCCTCTCTCCGAAAGAGGAGAAGGTGATCCGCATGCGGTTCGGTATCGGCTGTGAGCGCGAACATACCCTGGAAGAAATC
GGCCAGGAGTTCGACGTCACCCGCGAACGTATTCGCCAGATCGAAGCCAAAGCACTCCGCCAGCTACGCGCCCCGGAACG
GGCCCGCCGCCTGCGCGCGCTAATGGCAGCCCGTTAG

Upstream 100 bases:

>100_bases
GTGTCTACTTTTCCAATCACTTCGGGCCGTACCAGCAGACGCAAGCCATACTTTTTTCAGCTAGTTCCCCATTCCCTGAC
ATCCGATTGTTATCCGTCGC

Downstream 100 bases:

>100_bases
TCACTGTCGTTTCCTTCTCCGTTCCATTTCGCCGGGGCCTGAGCCGCCCCGGCGTTTCTTTTTGCCGTATCCTCCCGCGT
ACGGGTGCAGTTTCCGCTGT

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MPVGEVSLKVDQFEDQFLAENPEALGLSFEDDPKFFDPEAAHEADFLHPAPVEESIYTDDPVRVYLREMGAVPLLTREGE
VDLARRMERGKLRMQKAISRSALVQRVVVDVADQLKRANIEIESVVDLGDVEEGSPADVKVRNEAAKHFADIVVLHKKLQ
QLEDKVEATPLASKKPRKRLCAKLNRSKVETSLAIRRAPFRLIKWKEFAREIERAVDEISHLDSEIKRLEVRSNPTQQLR
LRELKREIKKREIAAGATLPELKHSLTVIRHGEAEAERAKKDLVEANLRLVVSVAKKYVNRGLHLLDLIQEGNIGLMRAA
DKFEYRRGYKFSTYATWWIRQAITRAIADQSRTIRIPVHMNESMNKFLRATRELEKELGRTPTNDEIGRRMDIPVEKVQK
LKTISRDPVSLETPVGRDGESALGDLIEDRWVGSPVDAVIESNVRDETAGILKTLSPKEEKVIRMRFGIGCEREHTLEEI
GQEFDVTRERIRQIEAKALRQLRAPERARRLRALMAAR

Sequences:

>Translated_518_residues
MPVGEVSLKVDQFEDQFLAENPEALGLSFEDDPKFFDPEAAHEADFLHPAPVEESIYTDDPVRVYLREMGAVPLLTREGE
VDLARRMERGKLRMQKAISRSALVQRVVVDVADQLKRANIEIESVVDLGDVEEGSPADVKVRNEAAKHFADIVVLHKKLQ
QLEDKVEATPLASKKPRKRLCAKLNRSKVETSLAIRRAPFRLIKWKEFAREIERAVDEISHLDSEIKRLEVRSNPTQQLR
LRELKREIKKREIAAGATLPELKHSLTVIRHGEAEAERAKKDLVEANLRLVVSVAKKYVNRGLHLLDLIQEGNIGLMRAA
DKFEYRRGYKFSTYATWWIRQAITRAIADQSRTIRIPVHMNESMNKFLRATRELEKELGRTPTNDEIGRRMDIPVEKVQK
LKTISRDPVSLETPVGRDGESALGDLIEDRWVGSPVDAVIESNVRDETAGILKTLSPKEEKVIRMRFGIGCEREHTLEEI
GQEFDVTRERIRQIEAKALRQLRAPERARRLRALMAAR
>Mature_517_residues
PVGEVSLKVDQFEDQFLAENPEALGLSFEDDPKFFDPEAAHEADFLHPAPVEESIYTDDPVRVYLREMGAVPLLTREGEV
DLARRMERGKLRMQKAISRSALVQRVVVDVADQLKRANIEIESVVDLGDVEEGSPADVKVRNEAAKHFADIVVLHKKLQQ
LEDKVEATPLASKKPRKRLCAKLNRSKVETSLAIRRAPFRLIKWKEFAREIERAVDEISHLDSEIKRLEVRSNPTQQLRL
RELKREIKKREIAAGATLPELKHSLTVIRHGEAEAERAKKDLVEANLRLVVSVAKKYVNRGLHLLDLIQEGNIGLMRAAD
KFEYRRGYKFSTYATWWIRQAITRAIADQSRTIRIPVHMNESMNKFLRATRELEKELGRTPTNDEIGRRMDIPVEKVQKL
KTISRDPVSLETPVGRDGESALGDLIEDRWVGSPVDAVIESNVRDETAGILKTLSPKEEKVIRMRFGIGCEREHTLEEIG
QEFDVTRERIRQIEAKALRQLRAPERARRLRALMAAR

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=261, Percent_Identity=58.6206896551724, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=44.4444444444444, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789871, Length=252, Percent_Identity=26.984126984127, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=27.0935960591133, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 59286; Mature: 59155

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVGEVSLKVDQFEDQFLAENPEALGLSFEDDPKFFDPEAAHEADFLHPAPVEESIYTDD
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCC
PVRVYLREMGAVPLLTREGEVDLARRMERGKLRMQKAISRSALVQRVVVDVADQLKRANI
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIESVVDLGDVEEGSPADVKVRNEAAKHFADIVVLHKKLQQLEDKVEATPLASKKPRKRL
CHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
CAKLNRSKVETSLAIRRAPFRLIKWKEFAREIERAVDEISHLDSEIKRLEVRSNPTQQLR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LRELKREIKKREIAAGATLPELKHSLTVIRHGEAEAERAKKDLVEANLRLVVSVAKKYVN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLHLLDLIQEGNIGLMRAADKFEYRRGYKFSTYATWWIRQAITRAIADQSRTIRIPVHM
CCHHHHHHHHCCCCCEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
NESMNKFLRATRELEKELGRTPTNDEIGRRMDIPVEKVQKLKTISRDPVSLETPVGRDGE
CHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH
SALGDLIEDRWVGSPVDAVIESNVRDETAGILKTLSPKEEKVIRMRFGIGCEREHTLEEI
HHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
GQEFDVTRERIRQIEAKALRQLRAPERARRLRALMAAR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVGEVSLKVDQFEDQFLAENPEALGLSFEDDPKFFDPEAAHEADFLHPAPVEESIYTDD
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCC
PVRVYLREMGAVPLLTREGEVDLARRMERGKLRMQKAISRSALVQRVVVDVADQLKRANI
HHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIESVVDLGDVEEGSPADVKVRNEAAKHFADIVVLHKKLQQLEDKVEATPLASKKPRKRL
CHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
CAKLNRSKVETSLAIRRAPFRLIKWKEFAREIERAVDEISHLDSEIKRLEVRSNPTQQLR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LRELKREIKKREIAAGATLPELKHSLTVIRHGEAEAERAKKDLVEANLRLVVSVAKKYVN
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLHLLDLIQEGNIGLMRAADKFEYRRGYKFSTYATWWIRQAITRAIADQSRTIRIPVHM
CCHHHHHHHHCCCCCEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
NESMNKFLRATRELEKELGRTPTNDEIGRRMDIPVEKVQKLKTISRDPVSLETPVGRDGE
CHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCH
SALGDLIEDRWVGSPVDAVIESNVRDETAGILKTLSPKEEKVIRMRFGIGCEREHTLEEI
HHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHH
GQEFDVTRERIRQIEAKALRQLRAPERARRLRALMAAR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]