Click here to switch to the map view.

The map label for this gene is guaD [H]

Identifier: 116619498

GI number: 116619498

Start: 457770

End: 459086

Strand: Direct

Name: guaD [H]

Synonym: Acid_0357

Alternate gene names: 116619498

Gene position: 457770-459086 (Clockwise)

Preceding gene: 116619497

Following gene: 116619499

Centisome position: 4.59

GC content: 65.15

Gene sequence:

>1317_bases
GTGAGCGAAATCCTGCGTGCCACCATTTTCCACGCGCCGCGCAATCCCTTCCGAACTCCGGGCTCGCTCGTCTTCCATGA
GGATGGCGCGCTGCTGCTCGCCGAAGGCCGCATTTCCGCCTGCGACGACTACTCCGTTGTGCGTAAGGTTAACCCACAGG
CGCCTGTCCGCGACTTGCGCGGCGGAGTCCTGCTGCCCGGCTTCATCGACACCCACGTCCACTACCCGCAGGTTCGCATC
CTCGGGTGCCTCGGCCACGGTCTGCTCGAATGGCTGCAGCGCTACACGCTTCCCGAGGAAGCAAAATTCGAAGACGTTGC
CTATGCCCGCACCATCGCCGGTGAATTCCTGCGCAGCCTCGTTGCGCACGGCACCACCACCGCGCTGGTTTTTGGCTCTC
ACTTCTCCGCCGCCACCGCTGAACTGTTCGCCGCCGCCAAAGTCTCCGGTCTGCGTATCGCCAGCGGAATGGTGCTCTCG
GATCGCTGCCTGCGCACCGAATTGCACCAGTCGCCCGAGAACGCGTATCGCGAAAGCAGCCGCCTGATCCGCGAATTCCA
CGGCGTGGGACGGCTCTTGTACGCCGTCACGCCGCGCTTCGCGCTCTCCTGCTCGGATGCCATGCTGGAAGTCTGCGGCG
CGCTGCTGCGCGAAAATCCCGGCCTCCGCTTTCAGACTCACCTCAATGAATCGCAGGCCGAAGTCAAAGAGGTCGCGCAC
CTTTTTCCCCAAGACGCCGATTACTTCGCCGTCTATGAACGCCACGGTCTCGCCAATCGCCACGCCGTCTTCGCGCACGG
CGTGCAGACCACGAATGCGGAGCTGGAGCGTATGGCGGCCTGTGGCAGCAGCGCGTCGCACTGCCCCGCCAGCAATGCCG
CGCTGGGCAGCGGCATTTTTCCCATGCGCCGGCACCTGGCGCGCGGCGTTCACTTTGCCCTCGGCAGCGATGTCGGCGGC
GGTACCGGTTTCGGCATGCTCAAGGAAGGGCTGCAGGCCTACCTCGCGCAACGCCTCGCGCTCGACGGCTTCCCCCTGGC
CGCGGAACATCTCCTCTACCTCGCGACCCGCGCCGGTGCCGGAGCCTTAGGCCTCGAAAACGAAATCGGCGATTTCACTC
CCGGCAAAGCCGCCGACCTGGTCTACGTGCGCGCGCCCGAAGGCAGTTCGCTCGCCGCCGTGACCCGCAACGCGCTCTCC
GCGGAACATCTGCTCGCGGCCATTTTTACCCTTGCCGATGAAGCCTGCATCCGCCAGGTCTGGGTGCAAGGCTCTCCGGT
CAAATGCGGTTTGCAGGAGGAACTCGCGTCCGCATGA

Upstream 100 bases:

>100_bases
TCGATAGCCCCGCCACTCCCGAGCGCGTCTTTTGGGCCATCCAGCGCGCACGCGGGGCCGCCACCGAGCCCGTGTCTTCC
GCGGTCGGAGCCGCGCAAGC

Downstream 100 bases:

>100_bases
CCATCGCCGAGGCCAATGAGCTGCGCCGCGAAGTCTTCGTTGCCGCGTTCGGGTGGATCTTCGAGCACTCGGCCTGGGTC
GCGAACCGCGCCTGGCAGCA

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSEILRATIFHAPRNPFRTPGSLVFHEDGALLLAEGRISACDDYSVVRKVNPQAPVRDLRGGVLLPGFIDTHVHYPQVRI
LGCLGHGLLEWLQRYTLPEEAKFEDVAYARTIAGEFLRSLVAHGTTTALVFGSHFSAATAELFAAAKVSGLRIASGMVLS
DRCLRTELHQSPENAYRESSRLIREFHGVGRLLYAVTPRFALSCSDAMLEVCGALLRENPGLRFQTHLNESQAEVKEVAH
LFPQDADYFAVYERHGLANRHAVFAHGVQTTNAELERMAACGSSASHCPASNAALGSGIFPMRRHLARGVHFALGSDVGG
GTGFGMLKEGLQAYLAQRLALDGFPLAAEHLLYLATRAGAGALGLENEIGDFTPGKAADLVYVRAPEGSSLAAVTRNALS
AEHLLAAIFTLADEACIRQVWVQGSPVKCGLQEELASA

Sequences:

>Translated_438_residues
MSEILRATIFHAPRNPFRTPGSLVFHEDGALLLAEGRISACDDYSVVRKVNPQAPVRDLRGGVLLPGFIDTHVHYPQVRI
LGCLGHGLLEWLQRYTLPEEAKFEDVAYARTIAGEFLRSLVAHGTTTALVFGSHFSAATAELFAAAKVSGLRIASGMVLS
DRCLRTELHQSPENAYRESSRLIREFHGVGRLLYAVTPRFALSCSDAMLEVCGALLRENPGLRFQTHLNESQAEVKEVAH
LFPQDADYFAVYERHGLANRHAVFAHGVQTTNAELERMAACGSSASHCPASNAALGSGIFPMRRHLARGVHFALGSDVGG
GTGFGMLKEGLQAYLAQRLALDGFPLAAEHLLYLATRAGAGALGLENEIGDFTPGKAADLVYVRAPEGSSLAAVTRNALS
AEHLLAAIFTLADEACIRQVWVQGSPVKCGLQEELASA
>Mature_437_residues
SEILRATIFHAPRNPFRTPGSLVFHEDGALLLAEGRISACDDYSVVRKVNPQAPVRDLRGGVLLPGFIDTHVHYPQVRIL
GCLGHGLLEWLQRYTLPEEAKFEDVAYARTIAGEFLRSLVAHGTTTALVFGSHFSAATAELFAAAKVSGLRIASGMVLSD
RCLRTELHQSPENAYRESSRLIREFHGVGRLLYAVTPRFALSCSDAMLEVCGALLRENPGLRFQTHLNESQAEVKEVAHL
FPQDADYFAVYERHGLANRHAVFAHGVQTTNAELERMAACGSSASHCPASNAALGSGIFPMRRHLARGVHFALGSDVGGG
TGFGMLKEGLQAYLAQRLALDGFPLAAEHLLYLATRAGAGALGLENEIGDFTPGKAADLVYVRAPEGSSLAAVTRNALSA
EHLLAAIFTLADEACIRQVWVQGSPVKCGLQEELASA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=375, Percent_Identity=30.4, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1789249, Length=415, Percent_Identity=38.7951807228916, Blast_Score=301, Evalue=7e-83,
Organism=Saccharomyces cerevisiae, GI6319963, Length=396, Percent_Identity=30.3030303030303, Blast_Score=171, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24643849, Length=394, Percent_Identity=31.9796954314721, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 47178; Mature: 47047

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEILRATIFHAPRNPFRTPGSLVFHEDGALLLAEGRISACDDYSVVRKVNPQAPVRDLR
CCHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHC
GGVLLPGFIDTHVHYPQVRILGCLGHGLLEWLQRYTLPEEAKFEDVAYARTIAGEFLRSL
CCEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
VAHGTTTALVFGSHFSAATAELFAAAKVSGLRIASGMVLSDRCLRTELHQSPENAYRESS
HHCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHH
RLIREFHGVGRLLYAVTPRFALSCSDAMLEVCGALLRENPGLRFQTHLNESQAEVKEVAH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
LFPQDADYFAVYERHGLANRHAVFAHGVQTTNAELERMAACGSSASHCPASNAALGSGIF
HCCCCCCCEEHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
PMRRHLARGVHFALGSDVGGGTGFGMLKEGLQAYLAQRLALDGFPLAAEHLLYLATRAGA
HHHHHHHHCHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
GALGLENEIGDFTPGKAADLVYVRAPEGSSLAAVTRNALSAEHLLAAIFTLADEACIRQV
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVQGSPVKCGLQEELASA
HCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
SEILRATIFHAPRNPFRTPGSLVFHEDGALLLAEGRISACDDYSVVRKVNPQAPVRDLR
CHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCHHHHHHCCCCCCHHHHC
GGVLLPGFIDTHVHYPQVRILGCLGHGLLEWLQRYTLPEEAKFEDVAYARTIAGEFLRSL
CCEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
VAHGTTTALVFGSHFSAATAELFAAAKVSGLRIASGMVLSDRCLRTELHQSPENAYRESS
HHCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHH
RLIREFHGVGRLLYAVTPRFALSCSDAMLEVCGALLRENPGLRFQTHLNESQAEVKEVAH
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
LFPQDADYFAVYERHGLANRHAVFAHGVQTTNAELERMAACGSSASHCPASNAALGSGIF
HCCCCCCCEEHHHHCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCH
PMRRHLARGVHFALGSDVGGGTGFGMLKEGLQAYLAQRLALDGFPLAAEHLLYLATRAGA
HHHHHHHHCHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
GALGLENEIGDFTPGKAADLVYVRAPEGSSLAAVTRNALSAEHLLAAIFTLADEACIRQV
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVQGSPVKCGLQEELASA
HCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]