Click here to switch to the map view.

The map label for this gene is trmFO

Identifier: 116619487

GI number: 116619487

Start: 441332

End: 442669

Strand: Direct

Name: trmFO

Synonym: Acid_0346

Alternate gene names: 116619487

Gene position: 441332-442669 (Clockwise)

Preceding gene: 116619486

Following gene: 116619488

Centisome position: 4.43

GC content: 66.37

Gene sequence:

>1338_bases
GTGAGCAAACCCATCCACATTATCGGCGGAGGACTCGCCGGCACCGAAGCCGCCTGGCAGCTCGCCCGCCGCGGCCTCGC
CTGCACGCTTCATGAGATGCGCCCCGTGCGCCCTACCCCCGCGCACCAGACCGACCGGCTCGCCGAGCTCGTCTGCAGCA
ATTCCCTGAAGAGCGAGTCCGAATCCACCGCCCCCTGGCTACTCAAAGAGGAGCTCCGCCGCCTCGATTCCCTCCTCCTC
CAGTGCGCCCAAAAAGCCCGTGTCCCCGGCGGCCACGCCCTTACCGTGGATCGCGATATCTTCGCCCGCGAAGTCACCGC
CGCCATTACCGCCGAGCCCCTCATCACGCTCCTCCGCGACGAAGTCTCTTCCCTCTCTGCCGATGCCATCTGGATCGTAG
CCAGCGGACCCCTCACCAGCGGACCCCTCGCCGAGGAAATCGCCCGCCTTACCGGCTCCGGCCGCCTCTACTTCTACGAC
AGCATCAGCCCCATCGTCGATGCCGAGTCCGTGGACACGTCCATCGCCTGGTGGGCCTCCCGCTACGGCAAATCCACCGA
CGGCACCGACGACTATCTCAACTGCCCTCTCGACCGCGGCCAGTACGAACACTTCGTGGACGAGCTCCTCAAGGCCGATT
CCGTCTCCGCCCACATTCCCGAAGACAACACCTGCTACTTCGAGGCCTGCCTTCCCATCGAGGAAATCGCCCGCCGCGGC
CGCGACACGCTCCGCTTCGGCCCCATGAAGCCCATGGGCCTCGACGATCCGCGCACCGGCCGCCGCCCTTGGGCCGTTGT
CCAGCTTCGCCAGGAGAACCTCCGCGCCGAGAGTTTCAATCTCGTCGGTTTTCAGAACCACATGAAGTTCCCCGAGCAGA
AGCGCGTCCTCCGCCTCATCCCCGGCCTTCAGAACGCCGAGTTCCTGCGCTACGGCCAGATTCACCGCAACACCTACATC
AACGCCCCCGCCCTTCTCACGGCCGCTCTCAACCTCCGCGCACGGCCCGAAATTTTCTTCGCCGGCCAGATTTCCGGCGT
CGAAGGCTACGTCGAATCCATTGCCACCGGACTCATGGCCGGCATCCACGCCGCCGCGCTCGCCACGGGCGATACTCCGC
GCCCCCTGCCGCGCCAGACCGCCCTGGGCTCGCTCTGTCACTACGTCTCCGGCGCCGATCCTAAGGACTACCAGCCCGCC
AACATCACCTTCGACCTCCTCCCCCAACTCGAGGAGGCCGAACGCCAGCGCCTTCGCCGCGACAAGAAGGCGCGCCACGC
CCTCGTCTGCCAGCGTGCGCTCGAAGCGCTCGAAGAGTATCGCCATGCCATCGTCTGA

Upstream 100 bases:

>100_bases
TGCGCTTCGATGAGGTCAGCGCCGTCTACGCCCTCTTCGGCTCCTTCTACGTCGGCGTCCGCGTCCCGGCAGCCGACCTC
GCCGGCATCCTCTGACCCGC

Downstream 100 bases:

>100_bases
ACCGTCTGAACTGGAACGGCAAATCGTCCTTTACCTCGAAGACCTCGCCCGCGCCGGCAATTCCGAGCACTCCATCCGCG
CCTACGAATCCGACCTCCGC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSKPIHIIGGGLAGTEAAWQLARRGLACTLHEMRPVRPTPAHQTDRLAELVCSNSLKSESESTAPWLLKEELRRLDSLLL
QCAQKARVPGGHALTVDRDIFAREVTAAITAEPLITLLRDEVSSLSADAIWIVASGPLTSGPLAEEIARLTGSGRLYFYD
SISPIVDAESVDTSIAWWASRYGKSTDGTDDYLNCPLDRGQYEHFVDELLKADSVSAHIPEDNTCYFEACLPIEEIARRG
RDTLRFGPMKPMGLDDPRTGRRPWAVVQLRQENLRAESFNLVGFQNHMKFPEQKRVLRLIPGLQNAEFLRYGQIHRNTYI
NAPALLTAALNLRARPEIFFAGQISGVEGYVESIATGLMAGIHAAALATGDTPRPLPRQTALGSLCHYVSGADPKDYQPA
NITFDLLPQLEEAERQRLRRDKKARHALVCQRALEALEEYRHAIV

Sequences:

>Translated_445_residues
MSKPIHIIGGGLAGTEAAWQLARRGLACTLHEMRPVRPTPAHQTDRLAELVCSNSLKSESESTAPWLLKEELRRLDSLLL
QCAQKARVPGGHALTVDRDIFAREVTAAITAEPLITLLRDEVSSLSADAIWIVASGPLTSGPLAEEIARLTGSGRLYFYD
SISPIVDAESVDTSIAWWASRYGKSTDGTDDYLNCPLDRGQYEHFVDELLKADSVSAHIPEDNTCYFEACLPIEEIARRG
RDTLRFGPMKPMGLDDPRTGRRPWAVVQLRQENLRAESFNLVGFQNHMKFPEQKRVLRLIPGLQNAEFLRYGQIHRNTYI
NAPALLTAALNLRARPEIFFAGQISGVEGYVESIATGLMAGIHAAALATGDTPRPLPRQTALGSLCHYVSGADPKDYQPA
NITFDLLPQLEEAERQRLRRDKKARHALVCQRALEALEEYRHAIV
>Mature_444_residues
SKPIHIIGGGLAGTEAAWQLARRGLACTLHEMRPVRPTPAHQTDRLAELVCSNSLKSESESTAPWLLKEELRRLDSLLLQ
CAQKARVPGGHALTVDRDIFAREVTAAITAEPLITLLRDEVSSLSADAIWIVASGPLTSGPLAEEIARLTGSGRLYFYDS
ISPIVDAESVDTSIAWWASRYGKSTDGTDDYLNCPLDRGQYEHFVDELLKADSVSAHIPEDNTCYFEACLPIEEIARRGR
DTLRFGPMKPMGLDDPRTGRRPWAVVQLRQENLRAESFNLVGFQNHMKFPEQKRVLRLIPGLQNAEFLRYGQIHRNTYIN
APALLTAALNLRARPEIFFAGQISGVEGYVESIATGLMAGIHAAALATGDTPRPLPRQTALGSLCHYVSGADPKDYQPAN
ITFDLLPQLEEAERQRLRRDKKARHALVCQRALEALEEYRHAIV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_SOLUE (Q02C58)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_821643.1
- ProteinModelPortal:   Q02C58
- SMR:   Q02C58
- STRING:   Q02C58
- GeneID:   4431596
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_0346
- NMPDR:   fig|234267.9.peg.329
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49363; Mature: 49232

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIHIIGGGLAGTEAAWQLARRGLACTLHEMRPVRPTPAHQTDRLAELVCSNSLKSES
CCCCEEEEECCCCCHHHHHHHHHCCCCEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCC
ESTAPWLLKEELRRLDSLLLQCAQKARVPGGHALTVDRDIFAREVTAAITAEPLITLLRD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
EVSSLSADAIWIVASGPLTSGPLAEEIARLTGSGRLYFYDSISPIVDAESVDTSIAWWAS
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHHHHHHH
RYGKSTDGTDDYLNCPLDRGQYEHFVDELLKADSVSAHIPEDNTCYFEACLPIEEIARRG
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHCC
RDTLRFGPMKPMGLDDPRTGRRPWAVVQLRQENLRAESFNLVGFQNHMKFPEQKRVLRLI
CCCEEECCCCCCCCCCCCCCCCCHHEEEEHHHCCCHHHEEEEEEHHHCCCCHHHHHHHHC
PGLQNAEFLRYGQIHRNTYINAPALLTAALNLRARPEIFFAGQISGVEGYVESIATGLMA
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
GIHAAALATGDTPRPLPRQTALGSLCHYVSGADPKDYQPANITFDLLPQLEEAERQRLRR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHH
DKKARHALVCQRALEALEEYRHAIV
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKPIHIIGGGLAGTEAAWQLARRGLACTLHEMRPVRPTPAHQTDRLAELVCSNSLKSES
CCCEEEEECCCCCHHHHHHHHHCCCCEEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCC
ESTAPWLLKEELRRLDSLLLQCAQKARVPGGHALTVDRDIFAREVTAAITAEPLITLLRD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHH
EVSSLSADAIWIVASGPLTSGPLAEEIARLTGSGRLYFYDSISPIVDAESVDTSIAWWAS
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCHHHHHHHH
RYGKSTDGTDDYLNCPLDRGQYEHFVDELLKADSVSAHIPEDNTCYFEACLPIEEIARRG
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHCC
RDTLRFGPMKPMGLDDPRTGRRPWAVVQLRQENLRAESFNLVGFQNHMKFPEQKRVLRLI
CCCEEECCCCCCCCCCCCCCCCCHHEEEEHHHCCCHHHEEEEEEHHHCCCCHHHHHHHHC
PGLQNAEFLRYGQIHRNTYINAPALLTAALNLRARPEIFFAGQISGVEGYVESIATGLMA
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHH
GIHAAALATGDTPRPLPRQTALGSLCHYVSGADPKDYQPANITFDLLPQLEEAERQRLRR
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHH
DKKARHALVCQRALEALEEYRHAIV
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA