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The map label for this gene is hemK [C]

Identifier: 116619370

GI number: 116619370

Start: 308238

End: 309704

Strand: Direct

Name: hemK [C]

Synonym: Acid_0228

Alternate gene names: 116619370

Gene position: 308238-309704 (Clockwise)

Preceding gene: 116619368

Following gene: 116619371

Centisome position: 3.09

GC content: 64.83

Gene sequence:

>1467_bases
GTGCGCGCCATGTTGGTGGAGTGCGGCTTCACGCCCGAGGCGATTTGCGATCGTCTGGGGATCGCCGCGCTGGAGACTTA
CGAGCCCAACCGGTTCCGCGATGCCGGCCTCGGGATCGCGGAACAGGCGGTGGATGTCCTGATCCTGCTCCTGATGGATG
GCGAGTTTGTGCCGGCCGAGATCGTGGAACGGTTGTTGCCGGCGGATGCGGTCCGGAAGTTGGAGGCGCTCGGATTGATT
GCTAGCGATCCGGAATGCCCGGACATGTGGTTCGGCGGCTGCATTTTGTTTCCTGCGCGAGGCATGTGGCTGGCGAGCGA
CCGGATCGCTGTCCCGGACCGCGATGCTCTCCCGGCTTCCGGCGACCTGGTATTTCCGGCAGCCATCGAGAATACGGTGG
CGTTCCTGGCCACGCTCCCGGAAACTCCGTGCACGGCGCTCCTCGACTTGGGCACCGGCTCCGGCATTGCCGCGCTGGAT
GGCGCAAAGTACGCGAGCCACACCTGGGGAACCGACATCGCAGCGCGGTCGGTACATTTTGCGGAGTTTAACCGGCGGCT
GAATGGCATCCAAAATGCCACCATGCTGCAGGGCGACCTGTACGCCCCCGTGGAAGGGCTCACTTTCGACCGGATCGTGG
CTCATCCTCCATACGTGCCGGCGCGCCAGAATGCCATGATTTTTCGGGATGGCGGAGAAGATGGCGAACAGATCCTGCGC
GGCATCGTGGAGGGCCTGCCGCGATTTCTGCGCCCCGGCGGCCGGTTTTACGGCTTCGTCGTGGCGGCGGATTGTGAGGG
TCAGTGGTTCGAGGAGCGCCTCCGGCTGTGGTTGGGGGGGGCGGAGACGGAGTTCGACCTGGTGCTCGCCGCCCACACGT
TGACGGAACCACGGGATCTGACGGCAAATTCGTTCCTGGGCCAGAATACGGCCTTGGAGGACATCCGCTACCGTCACGAG
ATCTGGCGGCGGCGCAAGGTGGAGTTCCTGTTCAACGGGAGTGTCCTCATCCGGCGCCACAATTCTGCGCGCCCGGCGAT
TACGGCGCGCGTGTTGACGGGCGAAGGCTTCACTCCACGGCACGTTGAATGGCTGTTGGAGTGGAGCACCGAGGTTCACG
ACGCCGAGCGCCTGGAGCGACTCATGGAAGTTCGGCCCTTGCTTTCGCCCCACGCGGAAATGGTCGTGACCCACCGGGTA
CGGGATTGCCAACTGGCTCCGGAGGTGTTTTCCGTGCGGAGCAACCGGCCGTTCGAGGCGGACTGCACGCTGGAGCCTTG
GCTGGTGCAGATCGTCGCGCAGTGTGACGGCAAGACCACCTGGCGCGAGCATTTCGAGAGGGCAAAGAGCGCAGGCGCGA
TCCCCGGGGCCGCACCGGCCGGCGAGTTCCTGGCGATCCTGGAGCCCCTGGTCTCTAACGGTCTCCTGTGGATCGCGGAA
AAGTCCCTTCCCGGGTTGTCCGGCTGA

Upstream 100 bases:

>100_bases
GTCACGATGGTATTCTCGCAGAGTGCCCGGAATCAATCCTCAACCGCACGTTTCGCATCGCATCGCACCGCGACTAGGCA
GCGACGAGGAGTTTGCCGCG

Downstream 100 bases:

>100_bases
CGTCTGATGCGGCGATGCTATCCTCTCCCCATGACACCGACGGACTCACAGCCGCACACCTGGCACCGGATCGCACCGCG
ACTGGGCAGTGACGCGGAGT

Product: methyltransferase small

Products: NA

Alternate protein names: Methyltransferase; Transferase; rRNA Or TRNA Methyltransferase; Methyltransferase Family Protein; Methylase; RRNA/TRNA Methylase; Methyltransferas; Methylase Of Polypeptide Chain Release Factor; N5-Glutamine Methyltransferase; HemK Family Methylase; Methylase Of Polypeptide Chain Release Factors-Like Protein; Methylase HemK And PrmA ; Ribonucleotide Methyltransferase

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MRAMLVECGFTPEAICDRLGIAALETYEPNRFRDAGLGIAEQAVDVLILLLMDGEFVPAEIVERLLPADAVRKLEALGLI
ASDPECPDMWFGGCILFPARGMWLASDRIAVPDRDALPASGDLVFPAAIENTVAFLATLPETPCTALLDLGTGSGIAALD
GAKYASHTWGTDIAARSVHFAEFNRRLNGIQNATMLQGDLYAPVEGLTFDRIVAHPPYVPARQNAMIFRDGGEDGEQILR
GIVEGLPRFLRPGGRFYGFVVAADCEGQWFEERLRLWLGGAETEFDLVLAAHTLTEPRDLTANSFLGQNTALEDIRYRHE
IWRRRKVEFLFNGSVLIRRHNSARPAITARVLTGEGFTPRHVEWLLEWSTEVHDAERLERLMEVRPLLSPHAEMVVTHRV
RDCQLAPEVFSVRSNRPFEADCTLEPWLVQIVAQCDGKTTWREHFERAKSAGAIPGAAPAGEFLAILEPLVSNGLLWIAE
KSLPGLSG

Sequences:

>Translated_488_residues
MRAMLVECGFTPEAICDRLGIAALETYEPNRFRDAGLGIAEQAVDVLILLLMDGEFVPAEIVERLLPADAVRKLEALGLI
ASDPECPDMWFGGCILFPARGMWLASDRIAVPDRDALPASGDLVFPAAIENTVAFLATLPETPCTALLDLGTGSGIAALD
GAKYASHTWGTDIAARSVHFAEFNRRLNGIQNATMLQGDLYAPVEGLTFDRIVAHPPYVPARQNAMIFRDGGEDGEQILR
GIVEGLPRFLRPGGRFYGFVVAADCEGQWFEERLRLWLGGAETEFDLVLAAHTLTEPRDLTANSFLGQNTALEDIRYRHE
IWRRRKVEFLFNGSVLIRRHNSARPAITARVLTGEGFTPRHVEWLLEWSTEVHDAERLERLMEVRPLLSPHAEMVVTHRV
RDCQLAPEVFSVRSNRPFEADCTLEPWLVQIVAQCDGKTTWREHFERAKSAGAIPGAAPAGEFLAILEPLVSNGLLWIAE
KSLPGLSG
>Mature_488_residues
MRAMLVECGFTPEAICDRLGIAALETYEPNRFRDAGLGIAEQAVDVLILLLMDGEFVPAEIVERLLPADAVRKLEALGLI
ASDPECPDMWFGGCILFPARGMWLASDRIAVPDRDALPASGDLVFPAAIENTVAFLATLPETPCTALLDLGTGSGIAALD
GAKYASHTWGTDIAARSVHFAEFNRRLNGIQNATMLQGDLYAPVEGLTFDRIVAHPPYVPARQNAMIFRDGGEDGEQILR
GIVEGLPRFLRPGGRFYGFVVAADCEGQWFEERLRLWLGGAETEFDLVLAAHTLTEPRDLTANSFLGQNTALEDIRYRHE
IWRRRKVEFLFNGSVLIRRHNSARPAITARVLTGEGFTPRHVEWLLEWSTEVHDAERLERLMEVRPLLSPHAEMVVTHRV
RDCQLAPEVFSVRSNRPFEADCTLEPWLVQIVAQCDGKTTWREHFERAKSAGAIPGAAPAGEFLAILEPLVSNGLLWIAE
KSLPGLSG

Specific function: Methylates The Translation Termination Release Factor Rf1 On Gln-235 And Rf2 On Gln-252. [C]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.- [C]

Molecular weight: Translated: 53940; Mature: 53940

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAMLVECGFTPEAICDRLGIAALETYEPNRFRDAGLGIAEQAVDVLILLLMDGEFVPAE
CCEEEEECCCCHHHHHHHHCCCEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHH
IVERLLPADAVRKLEALGLIASDPECPDMWFGGCILFPARGMWLASDRIAVPDRDALPAS
HHHHHCCHHHHHHHHHHCCEECCCCCCCCHHCCEEEECCCCEEEECCCEECCCCCCCCCC
GDLVFPAAIENTVAFLATLPETPCTALLDLGTGSGIAALDGAKYASHTWGTDIAARSVHF
CCEEEHHHHHHHHHHHHCCCCCCCHHEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHH
AEFNRRLNGIQNATMLQGDLYAPVEGLTFDRIVAHPPYVPARQNAMIFRDGGEDGEQILR
HHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHH
GIVEGLPRFLRPGGRFYGFVVAADCEGQWFEERLRLWLGGAETEFDLVLAAHTLTEPRDL
HHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCCCC
TANSFLGQNTALEDIRYRHEIWRRRKVEFLFNGSVLIRRHNSARPAITARVLTGEGFTPR
CHHHHCCCCCHHHHHHHHHHHHHHHCEEEEECCEEEEEECCCCCCEEEEEEEECCCCCHH
HVEWLLEWSTEVHDAERLERLMEVRPLLSPHAEMVVTHRVRDCQLAPEVFSVRSNRPFEA
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
DCTLEPWLVQIVAQCDGKTTWREHFERAKSAGAIPGAAPAGEFLAILEPLVSNGLLWIAE
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEEE
KSLPGLSG
CCCCCCCC
>Mature Secondary Structure
MRAMLVECGFTPEAICDRLGIAALETYEPNRFRDAGLGIAEQAVDVLILLLMDGEFVPAE
CCEEEEECCCCHHHHHHHHCCCEEECCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCHHH
IVERLLPADAVRKLEALGLIASDPECPDMWFGGCILFPARGMWLASDRIAVPDRDALPAS
HHHHHCCHHHHHHHHHHCCEECCCCCCCCHHCCEEEECCCCEEEECCCEECCCCCCCCCC
GDLVFPAAIENTVAFLATLPETPCTALLDLGTGSGIAALDGAKYASHTWGTDIAARSVHF
CCEEEHHHHHHHHHHHHCCCCCCCHHEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHH
AEFNRRLNGIQNATMLQGDLYAPVEGLTFDRIVAHPPYVPARQNAMIFRDGGEDGEQILR
HHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCHHHHHH
GIVEGLPRFLRPGGRFYGFVVAADCEGQWFEERLRLWLGGAETEFDLVLAAHTLTEPRDL
HHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEEEEHHCCCCCCC
TANSFLGQNTALEDIRYRHEIWRRRKVEFLFNGSVLIRRHNSARPAITARVLTGEGFTPR
CHHHHCCCCCHHHHHHHHHHHHHHHCEEEEECCEEEEEECCCCCCEEEEEEEECCCCCHH
HVEWLLEWSTEVHDAERLERLMEVRPLLSPHAEMVVTHRVRDCQLAPEVFSVRSNRPFEA
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCC
DCTLEPWLVQIVAQCDGKTTWREHFERAKSAGAIPGAAPAGEFLAILEPLVSNGLLWIAE
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEEE
KSLPGLSG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA