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The map label for this gene is garL [H]

Identifier: 116619342

GI number: 116619342

Start: 258413

End: 259219

Strand: Direct

Name: garL [H]

Synonym: Acid_0199

Alternate gene names: 116619342

Gene position: 258413-259219 (Clockwise)

Preceding gene: 116619341

Following gene: 116619343

Centisome position: 2.59

GC content: 65.55

Gene sequence:

>807_bases
ATGAGCGATCAGCAGATTTGGCGTAACCCGGTACGCGAGCGGCTGGAGGCCGGACAACCTGCATTCGGCGTGACCATCAC
GACGGCTTCGGTGGAAGCCGCCGCCCACGCCGCCACACTCGGCTTTCACTTTTTGTGGATTGAGATGGAGCACTCTCCAG
TCACGTTGGAGACGCTTCGCCTTATGGTGCTGGCCACGAGCGGCCGGCCCGCCGCGGTTTTCGCGCGCGTGCCGGTGGTG
GAGTTGTGGACGGCGAAGCGCGTTCTCGATCAGGGGGTGAGCGGCGTAATCTTCCCGTTTGCCGGTACCGCCGAAAAGGC
TGCGCGCGCCGCAGCGGCGTGCCGTTACCCTCCCGCAGGCATGCGCGGCTCGGGAGCCGGCGCGGCGGTTCGCACCTGGC
CGCAGCCGGGAAACTACTACGATTCGGCCGATCGCGAGATCATGACCATCTGCGTGGTGGAAGAGGCGTCGGCAGTGGAC
GAAATCGATGCCATCGCCGCCACTCCGGGCGTGGACGTACTCTTCATCGGGACCAGCGACCTTTCCTTCTCGCTTGGACT
GCGCGGCAGGCAGAACGAGCCCGCCTTGCAAGATGCGATCGCGCGGATTGCGGACGCGGCCAAGCGTCACGGGAAATTCC
TGGGCCGGCCGGCGGGAACTCCCGAAGAGGTCCGGCGCCACATGGAGCAGGGCTTCCTGTTGTTTCAGATGCCCACCGAG
ATCGGCCTGATGGAACTCGGCGCGCGGCAACTTCTGGAGCCGCTCGGAATCTCCGGCATCCCCGCCGGTCAACGCGCGAT
ATATTAG

Upstream 100 bases:

>100_bases
GGGCCCGCCCGTCGTCTGAAGCGCCGTGGATTGCAGTGGCTATCCCCGACCACCAAATCTCGCGCCGGAAAGAAGCAACC
GGCCGGTAGAATCGAAGGAC

Downstream 100 bases:

>100_bases
CTGCGTGACCAGAGCATTCGGATTCCTCCTCCTTGCGATCTCGCCGGCCTTCGCTGCCGACCTGACGCAGGCCACCGTGG
TGGCACCGGCCAGCCTGTCC

Product: HpcH/HpaI aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSDQQIWRNPVRERLEAGQPAFGVTITTASVEAAAHAATLGFHFLWIEMEHSPVTLETLRLMVLATSGRPAAVFARVPVV
ELWTAKRVLDQGVSGVIFPFAGTAEKAARAAAACRYPPAGMRGSGAGAAVRTWPQPGNYYDSADREIMTICVVEEASAVD
EIDAIAATPGVDVLFIGTSDLSFSLGLRGRQNEPALQDAIARIADAAKRHGKFLGRPAGTPEEVRRHMEQGFLLFQMPTE
IGLMELGARQLLEPLGISGIPAGQRAIY

Sequences:

>Translated_268_residues
MSDQQIWRNPVRERLEAGQPAFGVTITTASVEAAAHAATLGFHFLWIEMEHSPVTLETLRLMVLATSGRPAAVFARVPVV
ELWTAKRVLDQGVSGVIFPFAGTAEKAARAAAACRYPPAGMRGSGAGAAVRTWPQPGNYYDSADREIMTICVVEEASAVD
EIDAIAATPGVDVLFIGTSDLSFSLGLRGRQNEPALQDAIARIADAAKRHGKFLGRPAGTPEEVRRHMEQGFLLFQMPTE
IGLMELGARQLLEPLGISGIPAGQRAIY
>Mature_267_residues
SDQQIWRNPVRERLEAGQPAFGVTITTASVEAAAHAATLGFHFLWIEMEHSPVTLETLRLMVLATSGRPAAVFARVPVVE
LWTAKRVLDQGVSGVIFPFAGTAEKAARAAAACRYPPAGMRGSGAGAAVRTWPQPGNYYDSADREIMTICVVEEASAVDE
IDAIAATPGVDVLFIGTSDLSFSLGLRGRQNEPALQDAIARIADAAKRHGKFLGRPAGTPEEVRRHMEQGFLLFQMPTEI
GLMELGARQLLEPLGISGIPAGQRAIY

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=251, Percent_Identity=30.6772908366534, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788578, Length=217, Percent_Identity=33.6405529953917, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28748; Mature: 28617

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQQIWRNPVRERLEAGQPAFGVTITTASVEAAAHAATLGFHFLWIEMEHSPVTLETLR
CCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHEEEEEEEEECCCCCHHHHEE
LMVLATSGRPAAVFARVPVVELWTAKRVLDQGVSGVIFPFAGTAEKAARAAAACRYPPAG
EEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCC
MRGSGAGAAVRTWPQPGNYYDSADREIMTICVVEEASAVDEIDAIAATPGVDVLFIGTSD
CCCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCC
LSFSLGLRGRQNEPALQDAIARIADAAKRHGKFLGRPAGTPEEVRRHMEQGFLLFQMPTE
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCH
IGLMELGARQLLEPLGISGIPAGQRAIY
HHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDQQIWRNPVRERLEAGQPAFGVTITTASVEAAAHAATLGFHFLWIEMEHSPVTLETLR
CCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHEEEEEEEEECCCCCHHHHEE
LMVLATSGRPAAVFARVPVVELWTAKRVLDQGVSGVIFPFAGTAEKAARAAAACRYPPAG
EEEEECCCCCEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCC
MRGSGAGAAVRTWPQPGNYYDSADREIMTICVVEEASAVDEIDAIAATPGVDVLFIGTSD
CCCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEEEEECCC
LSFSLGLRGRQNEPALQDAIARIADAAKRHGKFLGRPAGTPEEVRRHMEQGFLLFQMPTE
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCH
IGLMELGARQLLEPLGISGIPAGQRAIY
HHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA