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The map label for this gene is dapE [H]

Identifier: 116619274

GI number: 116619274

Start: 170922

End: 172298

Strand: Direct

Name: dapE [H]

Synonym: Acid_0130

Alternate gene names: 116619274

Gene position: 170922-172298 (Clockwise)

Preceding gene: 116619273

Following gene: 116619275

Centisome position: 1.72

GC content: 62.67

Gene sequence:

>1377_bases
ATGTCCGCCGCCACCGATTCATTCGTGAACCAGAACCAGGCCCGGCTTCTGGAAGAGCTTAAGCAGTTCCTTCGCATCCC
CAGTATCAGTACCTTGCCCGAGAATCGCCCCGACGTGGAACGCGCGGCGGAATTCGTCGCTGGCGCCCTGCGCACCGCCG
GCATGGAGAATGTCGAACTTATCCAGACGGCCGGTCATCCCCTGGTCTACGCCGACTGGCTGCACGCTCCCGGCAAACCG
ACTGTTCTCTGCTACGGCCACTACGACGTACAGCCCGCCGACCCGCTGGAACTATGGACCTCTCCGCCCTTCGAGCCCGC
CGAGCGCGATGGCAATCTCTATGGCCGCGGCACAGCCGACGATAAGGGCCAGATGTACTCCCACATCAAGGCAATCGAGG
CCCTCCGCGCCGCTTCGCCCACCAGCAAACTGCCCGTAAACATCCGCTTTCTCGTGGAAGGCGAAGAAGAGGTCGGCGGC
GCCGCGATCGCCAAATTCGTCGCCGAGAATCCCGAGAGGCTCGCCGCCGATGTCGCCCTCGTCTCCGACACTGCGATGTA
CGCCGAGGGCATGCCGACCCTCTGCATCGGTTTGCGCGGCTTGATCTATATGGAGGTCGAAGCTGCCGGCCCCATGCGCG
ACCTGCACTCCGGCCTGTATGGGGGCGCTGCCCCCAATGCCGTCTTTGGCTTGATCGAACTGCTGTCGAAGGCGAAGAAT
GCCGACGGCGTGCTGCAGATTCCCGGCATCTACAACGACGTCGATGAACCCGCCGCCGCCGAAATCGCCAGTTGGAAAAA
CCTGCCCTTTCAGGAAGGCGAATTCCTCAATAAGGAGGTGGGCTCCACTCACCTCACGGGCGAGCAGAATCGAATGGTTC
TGGAGCGCGTCTGGTCGCGTCCCACATTCGAAGTCCACGGCGTCGCCGGAGGCTTCACCGCCGCCGGCGCAAAAACCGTA
ATCCCCGCCAAGGCTGTCGCCAAGGTAAGCTTCCGGATCGTCCCCCGCCAGAAGCCCGATGAGGTGATCAGCGCTTTCCG
TGAATGGGTGCACCGCCACACACCGAAAGGAATCAAGACCGAGGTGCGCGTCCTTAGCTCCGCTCCCGGCCTGGTCGTCA
ATCCCGACCACCCCGCGATCCGGATCGCGGCCCGCGCCTTCGGCGAAGTCTTCGGCAAGGAAACGGTCTTCATCCGCTCC
GGCGGCTCCATCCCCATCGTCGGCGATTTCGCCACTCACCTCGGCATCCCCACCATCCTGATGGGCTTCGGTCTTCCCGA
CGACGGCCTTCACTCCCCTAACGAAAAATACAAACTCGAGAACTACTTCCTCGGCATTCGCACCGTCGCCCGCTTCTTAG
AACAATACGGCGGCTGA

Upstream 100 bases:

>100_bases
ACCCGAAGTGCGCCGGATCCTTTCCAACGCCTGCTCGCGGTCGATTCGCCAGCCGCGCTCGATGACCGCCGTATAATGGT
CTCTCCACCCCGGAGAGATC

Downstream 100 bases:

>100_bases
CGAGACCGTAAAGAGCGATCTACACTTATCCCTTATTCCCACTTACCTGCAGCAGCGCGAATACTACCGCCAGCGCTACT
CCCGTCACGGCCGTTCCCGC

Product: peptidase M20

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSAATDSFVNQNQARLLEELKQFLRIPSISTLPENRPDVERAAEFVAGALRTAGMENVELIQTAGHPLVYADWLHAPGKP
TVLCYGHYDVQPADPLELWTSPPFEPAERDGNLYGRGTADDKGQMYSHIKAIEALRAASPTSKLPVNIRFLVEGEEEVGG
AAIAKFVAENPERLAADVALVSDTAMYAEGMPTLCIGLRGLIYMEVEAAGPMRDLHSGLYGGAAPNAVFGLIELLSKAKN
ADGVLQIPGIYNDVDEPAAAEIASWKNLPFQEGEFLNKEVGSTHLTGEQNRMVLERVWSRPTFEVHGVAGGFTAAGAKTV
IPAKAVAKVSFRIVPRQKPDEVISAFREWVHRHTPKGIKTEVRVLSSAPGLVVNPDHPAIRIAARAFGEVFGKETVFIRS
GGSIPIVGDFATHLGIPTILMGFGLPDDGLHSPNEKYKLENYFLGIRTVARFLEQYGG

Sequences:

>Translated_458_residues
MSAATDSFVNQNQARLLEELKQFLRIPSISTLPENRPDVERAAEFVAGALRTAGMENVELIQTAGHPLVYADWLHAPGKP
TVLCYGHYDVQPADPLELWTSPPFEPAERDGNLYGRGTADDKGQMYSHIKAIEALRAASPTSKLPVNIRFLVEGEEEVGG
AAIAKFVAENPERLAADVALVSDTAMYAEGMPTLCIGLRGLIYMEVEAAGPMRDLHSGLYGGAAPNAVFGLIELLSKAKN
ADGVLQIPGIYNDVDEPAAAEIASWKNLPFQEGEFLNKEVGSTHLTGEQNRMVLERVWSRPTFEVHGVAGGFTAAGAKTV
IPAKAVAKVSFRIVPRQKPDEVISAFREWVHRHTPKGIKTEVRVLSSAPGLVVNPDHPAIRIAARAFGEVFGKETVFIRS
GGSIPIVGDFATHLGIPTILMGFGLPDDGLHSPNEKYKLENYFLGIRTVARFLEQYGG
>Mature_457_residues
SAATDSFVNQNQARLLEELKQFLRIPSISTLPENRPDVERAAEFVAGALRTAGMENVELIQTAGHPLVYADWLHAPGKPT
VLCYGHYDVQPADPLELWTSPPFEPAERDGNLYGRGTADDKGQMYSHIKAIEALRAASPTSKLPVNIRFLVEGEEEVGGA
AIAKFVAENPERLAADVALVSDTAMYAEGMPTLCIGLRGLIYMEVEAAGPMRDLHSGLYGGAAPNAVFGLIELLSKAKNA
DGVLQIPGIYNDVDEPAAAEIASWKNLPFQEGEFLNKEVGSTHLTGEQNRMVLERVWSRPTFEVHGVAGGFTAAGAKTVI
PAKAVAKVSFRIVPRQKPDEVISAFREWVHRHTPKGIKTEVRVLSSAPGLVVNPDHPAIRIAARAFGEVFGKETVFIRSG
GSIPIVGDFATHLGIPTILMGFGLPDDGLHSPNEKYKLENYFLGIRTVARFLEQYGG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=473, Percent_Identity=33.8266384778013, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI21071039, Length=468, Percent_Identity=31.8376068376068, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI271398277, Length=318, Percent_Identity=33.0188679245283, Blast_Score=168, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17563146, Length=465, Percent_Identity=33.5483870967742, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI193211059, Length=463, Percent_Identity=33.2613390928726, Blast_Score=241, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6321159, Length=469, Percent_Identity=30.4904051172708, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319758, Length=451, Percent_Identity=25.2771618625277, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24585880, Length=471, Percent_Identity=35.8811040339703, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 49627; Mature: 49496

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAATDSFVNQNQARLLEELKQFLRIPSISTLPENRPDVERAAEFVAGALRTAGMENVEL
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
IQTAGHPLVYADWLHAPGKPTVLCYGHYDVQPADPLELWTSPPFEPAERDGNLYGRGTAD
HHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCEEECCCCC
DKGQMYSHIKAIEALRAASPTSKLPVNIRFLVEGEEEVGGAAIAKFVAENPERLAADVAL
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHCHHHHHHHHHCCHHHHHHHHHH
VSDTAMYAEGMPTLCIGLRGLIYMEVEAAGPMRDLHSGLYGGAAPNAVFGLIELLSKAKN
HHHHHHHHCCCCHHHHHCCCEEEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
ADGVLQIPGIYNDVDEPAAAEIASWKNLPFQEGEFLNKEVGSTHLTGEQNRMVLERVWSR
CCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCC
PTFEVHGVAGGFTAAGAKTVIPAKAVAKVSFRIVPRQKPDEVISAFREWVHRHTPKGIKT
CCEEEECCCCCCHHCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHH
EVRVLSSAPGLVVNPDHPAIRIAARAFGEVFGKETVFIRSGGSIPIVGDFATHLGIPTIL
HHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEECHHHHHCHHHHH
MGFGLPDDGLHSPNEKYKLENYFLGIRTVARFLEQYGG
HHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAATDSFVNQNQARLLEELKQFLRIPSISTLPENRPDVERAAEFVAGALRTAGMENVEL
CCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
IQTAGHPLVYADWLHAPGKPTVLCYGHYDVQPADPLELWTSPPFEPAERDGNLYGRGTAD
HHHCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCCCCEEECCCCC
DKGQMYSHIKAIEALRAASPTSKLPVNIRFLVEGEEEVGGAAIAKFVAENPERLAADVAL
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHCHHHHHHHHHCCHHHHHHHHHH
VSDTAMYAEGMPTLCIGLRGLIYMEVEAAGPMRDLHSGLYGGAAPNAVFGLIELLSKAKN
HHHHHHHHCCCCHHHHHCCCEEEEEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
ADGVLQIPGIYNDVDEPAAAEIASWKNLPFQEGEFLNKEVGSTHLTGEQNRMVLERVWSR
CCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCC
PTFEVHGVAGGFTAAGAKTVIPAKAVAKVSFRIVPRQKPDEVISAFREWVHRHTPKGIKT
CCEEEECCCCCCHHCCCCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHH
EVRVLSSAPGLVVNPDHPAIRIAARAFGEVFGKETVFIRSGGSIPIVGDFATHLGIPTIL
HHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEECHHHHHCHHHHH
MGFGLPDDGLHSPNEKYKLENYFLGIRTVARFLEQYGG
HHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA