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The map label for this gene is btuB [H]

Identifier: 116619266

GI number: 116619266

Start: 163659

End: 165839

Strand: Direct

Name: btuB [H]

Synonym: Acid_0122

Alternate gene names: 116619266

Gene position: 163659-165839 (Clockwise)

Preceding gene: 116619264

Following gene: 116619267

Centisome position: 1.64

GC content: 63.23

Gene sequence:

>2181_bases
ATGCTTAGAAACGTCGCAGGCCTTTTGGCCTTTTGTTCGTTCTTTTCCGCCGGTCTTCGCGCTGCCGAGATCAAAGGCAA
AATCATCGACCCTTCCGGAGCGCCCGTCGCCGGCGCCCAGATAGCCGCCGTTAACCGTCTCGGCGTAATCATTCGCACCA
CTGCGTCGCTCACCGGCGGCTTCCAATTGGATGCCCCCGAAACGCCGGATCTGCGCATCGTCGTCACCGCGGCCGGATTC
AGTACTCGCACCCTGCCGCCTGCCGAGGCCGCCACCGTGCAGCTTGATATCGCCCCGCAGGTGGATTCCGTGGAAGTTGT
CGGTTCCACCATCGAGATCCCCACCACGCTCCAGGCGTCCAGCGTGGACATCATCACCAACACGCAGCTCCGCCAGCGCA
ATGAGCCCTTCGCGATGGACCTGCTCCGCTATGTGCCCGGCGTGGCCTTTAATCAATCCGGTGCGCCCGGCGGCGTGACC
AGCCTCTTCCTGCGCGGCGGCAATGCCAACATGAACCTGGTGCAGATCGATGGCGTCCCGGTGATCGGTTTCGGCGGCGG
CCTCGGCTTCGATTTCGCCCACATCCCAGCCGAAGCAGTGGACCACATCGAGCTTGTCCGCGGCGCTCAATCCGCGGTCT
ACGGGTCCTACGCCAACTCCGGCGTGGTGGATTTCGTAACCCGCCAGCCCCAGTCCGCTCCGCAGTTGGACCTGCTCGCC
GAGGGCGGCAGCCACAACGAGCGCCGCTTCGGTATCACCGCCACCGGCAGCCTCGCCGGCTTTGGCCTGCTCGTCTCCGC
CTCCCGCTTCGATACCGATGGCGTCGTCGCCAATAACGATTACCGCAATGAAGACGTACTTCTGAATGTCACCCGCCGCT
TCGGCCGCCAGAGCCTCGCCCTCCACGGATACTTCGATTCCAATGATGTTGGCGAGCCCGGCCCCTGGGGCTCCAACCCG
CTCCACATCTTCACGGGCATCGACCACGTCAGCCGCGGCAAGAACAATTTCTCCGAGTACGGCGCGCACTATGAGGCCGG
CCTCTCCGCGCGCGTTCGCACCGAGCTCACCGGCAGCTTCTTCCTGGCCAACAACGGCTACGTCAGCCAGTACGGCTTCA
GCTATAACCAGGATATTCGCGGCCTCGGCGAATCGCGCACCATCGTGAGCATCTCGCCCCATGACACTGCCTCCTTCGGC
GTCACCGCGGGCCGCGAGCAGGTGACCAATACTTACATCACCGACTCCTCGTTTTCCACCTTCCCCATCAAGCGCAACGA
AATCGCCATCTACGCCGAGAATCGCTACGAGATCGGCAACCGCCTCTTCCTGAGCGCCGGATTGCGCGGCGAATTCTTCC
GCACCGCCTCCGTCCCCTCCGACGGCTTTTCGCGACCCTTCTTCCCGCAGACCAACATCTCGCGCGCCAACCCCAAGTTC
TCCGCCGCGTATGCCGTGCCCAAAGGCACTCGTCTGCACGCGTCGTTCGGTATGGGCGTTCGCCCTCCGTCGGGCTTCGA
GCTGGCGTTCACCAACAATCCTGCCCTGCGCCCCGAGCGCACTCGCAGTTTCGATGCGGGTATCGAACAGAGGCTCGGCA
AGTTCCTTGTGCTGGACGGCACGTATTTCTATAACCGCTACTACGATCTCATCGTGACCCTTGGCGGCTCGCTCACCACC
CTAAGCCATTATAAGTCTGCCAACCTGGCCAACTCGCGGGCTCAAGGCGCCGAGTTCTCGGCGCGCCTCCGCCCCGCTCG
GTGGGTCTTCGTCACCGGCTCGTACACCCTGCTCGAAACGCGCATCTTGTCGCTGGACGGCTCCAGCAATCAGGCGCCGC
TCCCATTCACTGTGGGCCAGCAACTCACGCGGAGGCCCGAAAACTCGGGCAGCCTGGTCGCGACTTTCACTCACGGCCGC
CTCGCCGCCGATCTCACCGGCTACTTCCGCGGCAAGACGCTCTTCGAGGAACCCTCCTACGGCGCCAGCAGCGGTCTCTT
CTGGGACCCCGGCTTCGCGAACGTGGGCGTCAACGTGAATTATTCCCTCGCCCACGGTGTCGCCGCCTACGCCCACCTCC
GCAACGCTCTCAACAAAGACTACGAGGAGATCTATGGCTTCCCCTCCCCTAAACTAAACTTCGTAGCCGGCCTGAAATGG
ACGTTGGCTAAGGGACAGTAA

Upstream 100 bases:

>100_bases
CCCCCGGGGAACGGGAAGGCCGTACGATTTGACGATGCGCAAGTCAGGAGACCGGCCAGCGCGTAACCAAACAATTCGGC
TCTCGAGGGAGGAGCCAGAA

Downstream 100 bases:

>100_bases
GATGTTCTCGCAGAGACGCAGAGCCGCAGAGAAGCGCCAAGAAGAATTTCTTCCTTGGTTCTCTTTGCGTTACTTTGCGG
CTTTGCGGCTTTGCGAGAAA

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MLRNVAGLLAFCSFFSAGLRAAEIKGKIIDPSGAPVAGAQIAAVNRLGVIIRTTASLTGGFQLDAPETPDLRIVVTAAGF
STRTLPPAEAATVQLDIAPQVDSVEVVGSTIEIPTTLQASSVDIITNTQLRQRNEPFAMDLLRYVPGVAFNQSGAPGGVT
SLFLRGGNANMNLVQIDGVPVIGFGGGLGFDFAHIPAEAVDHIELVRGAQSAVYGSYANSGVVDFVTRQPQSAPQLDLLA
EGGSHNERRFGITATGSLAGFGLLVSASRFDTDGVVANNDYRNEDVLLNVTRRFGRQSLALHGYFDSNDVGEPGPWGSNP
LHIFTGIDHVSRGKNNFSEYGAHYEAGLSARVRTELTGSFFLANNGYVSQYGFSYNQDIRGLGESRTIVSISPHDTASFG
VTAGREQVTNTYITDSSFSTFPIKRNEIAIYAENRYEIGNRLFLSAGLRGEFFRTASVPSDGFSRPFFPQTNISRANPKF
SAAYAVPKGTRLHASFGMGVRPPSGFELAFTNNPALRPERTRSFDAGIEQRLGKFLVLDGTYFYNRYYDLIVTLGGSLTT
LSHYKSANLANSRAQGAEFSARLRPARWVFVTGSYTLLETRILSLDGSSNQAPLPFTVGQQLTRRPENSGSLVATFTHGR
LAADLTGYFRGKTLFEEPSYGASSGLFWDPGFANVGVNVNYSLAHGVAAYAHLRNALNKDYEEIYGFPSPKLNFVAGLKW
TLAKGQ

Sequences:

>Translated_726_residues
MLRNVAGLLAFCSFFSAGLRAAEIKGKIIDPSGAPVAGAQIAAVNRLGVIIRTTASLTGGFQLDAPETPDLRIVVTAAGF
STRTLPPAEAATVQLDIAPQVDSVEVVGSTIEIPTTLQASSVDIITNTQLRQRNEPFAMDLLRYVPGVAFNQSGAPGGVT
SLFLRGGNANMNLVQIDGVPVIGFGGGLGFDFAHIPAEAVDHIELVRGAQSAVYGSYANSGVVDFVTRQPQSAPQLDLLA
EGGSHNERRFGITATGSLAGFGLLVSASRFDTDGVVANNDYRNEDVLLNVTRRFGRQSLALHGYFDSNDVGEPGPWGSNP
LHIFTGIDHVSRGKNNFSEYGAHYEAGLSARVRTELTGSFFLANNGYVSQYGFSYNQDIRGLGESRTIVSISPHDTASFG
VTAGREQVTNTYITDSSFSTFPIKRNEIAIYAENRYEIGNRLFLSAGLRGEFFRTASVPSDGFSRPFFPQTNISRANPKF
SAAYAVPKGTRLHASFGMGVRPPSGFELAFTNNPALRPERTRSFDAGIEQRLGKFLVLDGTYFYNRYYDLIVTLGGSLTT
LSHYKSANLANSRAQGAEFSARLRPARWVFVTGSYTLLETRILSLDGSSNQAPLPFTVGQQLTRRPENSGSLVATFTHGR
LAADLTGYFRGKTLFEEPSYGASSGLFWDPGFANVGVNVNYSLAHGVAAYAHLRNALNKDYEEIYGFPSPKLNFVAGLKW
TLAKGQ
>Mature_726_residues
MLRNVAGLLAFCSFFSAGLRAAEIKGKIIDPSGAPVAGAQIAAVNRLGVIIRTTASLTGGFQLDAPETPDLRIVVTAAGF
STRTLPPAEAATVQLDIAPQVDSVEVVGSTIEIPTTLQASSVDIITNTQLRQRNEPFAMDLLRYVPGVAFNQSGAPGGVT
SLFLRGGNANMNLVQIDGVPVIGFGGGLGFDFAHIPAEAVDHIELVRGAQSAVYGSYANSGVVDFVTRQPQSAPQLDLLA
EGGSHNERRFGITATGSLAGFGLLVSASRFDTDGVVANNDYRNEDVLLNVTRRFGRQSLALHGYFDSNDVGEPGPWGSNP
LHIFTGIDHVSRGKNNFSEYGAHYEAGLSARVRTELTGSFFLANNGYVSQYGFSYNQDIRGLGESRTIVSISPHDTASFG
VTAGREQVTNTYITDSSFSTFPIKRNEIAIYAENRYEIGNRLFLSAGLRGEFFRTASVPSDGFSRPFFPQTNISRANPKF
SAAYAVPKGTRLHASFGMGVRPPSGFELAFTNNPALRPERTRSFDAGIEQRLGKFLVLDGTYFYNRYYDLIVTLGGSLTT
LSHYKSANLANSRAQGAEFSARLRPARWVFVTGSYTLLETRILSLDGSSNQAPLPFTVGQQLTRRPENSGSLVATFTHGR
LAADLTGYFRGKTLFEEPSYGASSGLFWDPGFANVGVNVNYSLAHGVAAYAHLRNALNKDYEEIYGFPSPKLNFVAGLKW
TLAKGQ

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=163, Percent_Identity=31.2883435582822, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788478, Length=250, Percent_Identity=26.4, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 78194; Mature: 78194

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNVAGLLAFCSFFSAGLRAAEIKGKIIDPSGAPVAGAQIAAVNRLGVIIRTTASLTGG
CCCHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCCCCEEEEEHHCEEEEEEECCCCCC
FQLDAPETPDLRIVVTAAGFSTRTLPPAEAATVQLDIAPQVDSVEVVGSTIEIPTTLQAS
EEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEECCEEECCCEECCC
SVDIITNTQLRQRNEPFAMDLLRYVPGVAFNQSGAPGGVTSLFLRGGNANMNLVQIDGVP
CEEEEECCHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCEEEEEECCEE
VIGFGGGLGFDFAHIPAEAVDHIELVRGAQSAVYGSYANSGVVDFVTRQPQSAPQLDLLA
EEEECCCCCCCHHHCCHHHHHHHHHHHCCHHHEECCCCCCCEEEEEECCCCCCCCEEEEE
EGGSHNERRFGITATGSLAGFGLLVSASRFDTDGVVANNDYRNEDVLLNVTRRFGRQSLA
CCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEEECCCCCCCCEEEEEHHHCCCCEEE
LHGYFDSNDVGEPGPWGSNPLHIFTGIDHVSRGKNNFSEYGAHYEAGLSARVRTELTGSF
EEEEECCCCCCCCCCCCCCCEEEEECCHHHHCCCCCHHHHCCCHHCCCCEEEEEEECCEE
FLANNGYVSQYGFSYNQDIRGLGESRTIVSISPHDTASFGVTAGREQVTNTYITDSSFST
EEECCCEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCEEEEECCCCEE
FPIKRNEIAIYAENRYEIGNRLFLSAGLRGEFFRTASVPSDGFSRPFFPQTNISRANPKF
CCEECCEEEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
SAAYAVPKGTRLHASFGMGVRPPSGFELAFTNNPALRPERTRSFDAGIEQRLGKFLVLDG
EEEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCHHCCHHHHCCCEEEEEC
TYFYNRYYDLIVTLGGSLTTLSHYKSANLANSRAQGAEFSARLRPARWVFVTGSYTLLET
CHHHHHEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEE
RILSLDGSSNQAPLPFTVGQQLTRRPENSGSLVATFTHGRLAADLTGYFRGKTLFEEPSY
EEEEECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCEEEEHHHHHHCCCEEECCCCC
GASSGLFWDPGFANVGVNVNYSLAHGVAAYAHLRNALNKDYEEIYGFPSPKLNFVAGLKW
CCCCCCEECCCCCEECEEEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECEEE
TLAKGQ
EEECCC
>Mature Secondary Structure
MLRNVAGLLAFCSFFSAGLRAAEIKGKIIDPSGAPVAGAQIAAVNRLGVIIRTTASLTGG
CCCHHHHHHHHHHHHHHCCEEEECCCEEECCCCCCCCCCEEEEEHHCEEEEEEECCCCCC
FQLDAPETPDLRIVVTAAGFSTRTLPPAEAATVQLDIAPQVDSVEVVGSTIEIPTTLQAS
EEECCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEEECCEEECCCEECCC
SVDIITNTQLRQRNEPFAMDLLRYVPGVAFNQSGAPGGVTSLFLRGGNANMNLVQIDGVP
CEEEEECCHHHHCCCCHHHHHHHHCCCEEECCCCCCCCCEEEEEECCCCCEEEEEECCEE
VIGFGGGLGFDFAHIPAEAVDHIELVRGAQSAVYGSYANSGVVDFVTRQPQSAPQLDLLA
EEEECCCCCCCHHHCCHHHHHHHHHHHCCHHHEECCCCCCCEEEEEECCCCCCCCEEEEE
EGGSHNERRFGITATGSLAGFGLLVSASRFDTDGVVANNDYRNEDVLLNVTRRFGRQSLA
CCCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCEEECCCCCCCCEEEEEHHHCCCCEEE
LHGYFDSNDVGEPGPWGSNPLHIFTGIDHVSRGKNNFSEYGAHYEAGLSARVRTELTGSF
EEEEECCCCCCCCCCCCCCCEEEEECCHHHHCCCCCHHHHCCCHHCCCCEEEEEEECCEE
FLANNGYVSQYGFSYNQDIRGLGESRTIVSISPHDTASFGVTAGREQVTNTYITDSSFST
EEECCCEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHCEEEEECCCCEE
FPIKRNEIAIYAENRYEIGNRLFLSAGLRGEFFRTASVPSDGFSRPFFPQTNISRANPKF
CCEECCEEEEEECCCCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
SAAYAVPKGTRLHASFGMGVRPPSGFELAFTNNPALRPERTRSFDAGIEQRLGKFLVLDG
EEEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCHHCCHHHHCCCEEEEEC
TYFYNRYYDLIVTLGGSLTTLSHYKSANLANSRAQGAEFSARLRPARWVFVTGSYTLLET
CHHHHHEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCCCCEECCCEEEEEEECCEEEEEE
RILSLDGSSNQAPLPFTVGQQLTRRPENSGSLVATFTHGRLAADLTGYFRGKTLFEEPSY
EEEEECCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCEEEEHHHHHHCCCEEECCCCC
GASSGLFWDPGFANVGVNVNYSLAHGVAAYAHLRNALNKDYEEIYGFPSPKLNFVAGLKW
CCCCCCEECCCCCEECEEEEEHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEECEEE
TLAKGQ
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA