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The map label for this gene is gdhA [H]

Identifier: 116619184

GI number: 116619184

Start: 61055

End: 62317

Strand: Direct

Name: gdhA [H]

Synonym: Acid_0039

Alternate gene names: 116619184

Gene position: 61055-62317 (Clockwise)

Preceding gene: 116619183

Following gene: 116619185

Centisome position: 0.61

GC content: 61.84

Gene sequence:

>1263_bases
ATGTTAGCAACCGCAACCACTCCAATGAACGCGTACGACGTCGCGTTGGAGAATTTTGACCTGGCGGCAAACGCGCTGGG
ACTAGACGACAGCATCCGGGCCATGATCAAGTACCCGGAGCGCATTCTCAGCGTGAGCGTGCCAGTCCGGATGGACACCG
GGAAGATTGTCCGCTTCGAAGGCTATCGCGTGCAGCACAGCACGATGCGTGGTCCGGCGAAGGGCGGAATCCGCTTCCAT
CCGAATGTCACGATGGACGAGGTGAAAGCGCTGGCCACGTGGATGACGTGGAAGTGCGCCGTGGTCAACATCCCTTACGG
CGGCGGCAAGGGCGGCGTGACCTGTAACCCGAAGGAACTGTCGATGGGCGAACTCGAACGGATGACCCGCCGGTACGCGA
GCTCGATCCTGCCGATCATCGGGCCGGAGAAGGACATTCCGGCGCCGGACGTCTACACGACCCCGCAGATCATGGCGTGG
ATCATGGACACGTACAGCATGAATAAAGGATATCCGGTGCACGGCGTGGTGACGGGCAAGCCGCTCTCCATAGGTGGATC
GCTCGGACGCAACGAAGCGACGGCGCGCGGCGTGTTTTACACCACGATGAGCAGCTGTGAGCACCTGGGGATCCAACTGG
CCGGATCGCGGGTGGTGGTGCAGGGCTTCGGGAACGCCGGGGCGATCGCCGCCGACTTGTTCCACGGCGCTGGCGCTAAG
GTGCTGGCGGTGAGCGACACCTCGGGCTGCATCTTCAACAAAAACGGCCTGCATATTCCGGCGGTGGTGGCTTACAAGGC
GCGGACGGGGCGTCTGGAAGGCTTCCCGGAAGCAACGCGGATTACGCCGGCGGAGTTGTTGGCGCTGGAATGCGAAATCC
TCGTGCCGGCGGCGCTGGAGAACGCGATCACGGAAGAGAATGCGCATACCATCCACGCGAAGATCATCAGCGAAGCGGCC
AACGGGCCGGTGACTCCGGAAGCGGACCGCATTCTCGGATCGAAGGGCATCTTCCTGATTCCGGACATCCTTTGCAATGC
GGGCGGCGTGACGGTCAGTTACTTCGAATGGGTGCAGGACGAGAACCACCTGTTCTGGGACGAGCAGGACGTGAACGCGA
AACTGGAGAAGATCATGAAGCGCTCCTTCGCGGACGTGCTCAAGATCCACCTGGATAAGAAGGTGGATATGCGCCTGGCG
GCAAATATGCTCGGCGTGTCGCGCGTTGCGGAAGCCTGCAAGGTCCGGGGGCTGTACCCGTAG

Upstream 100 bases:

>100_bases
ATGCGCCCGTAAAGCACCCCCTTGACGTGGTGCCTGTTTGGGAATACTATCCGACTGGAAACGACTTTACCGAGTCTGAA
TATCGGAAACGAGGTATGCC

Downstream 100 bases:

>100_bases
GCCCCGGGGCCATAAAGCAGCGTGTAACACCACGGGCCGGGCATGCAGGGCGCATGTCCGGCCCGTGCCGTGTTCAAATA
GGGAGGAAAGAAACCAAAAT

Product: glutamate dehydrogenase (NADP)

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLATATTPMNAYDVALENFDLAANALGLDDSIRAMIKYPERILSVSVPVRMDTGKIVRFEGYRVQHSTMRGPAKGGIRFH
PNVTMDEVKALATWMTWKCAVVNIPYGGGKGGVTCNPKELSMGELERMTRRYASSILPIIGPEKDIPAPDVYTTPQIMAW
IMDTYSMNKGYPVHGVVTGKPLSIGGSLGRNEATARGVFYTTMSSCEHLGIQLAGSRVVVQGFGNAGAIAADLFHGAGAK
VLAVSDTSGCIFNKNGLHIPAVVAYKARTGRLEGFPEATRITPAELLALECEILVPAALENAITEENAHTIHAKIISEAA
NGPVTPEADRILGSKGIFLIPDILCNAGGVTVSYFEWVQDENHLFWDEQDVNAKLEKIMKRSFADVLKIHLDKKVDMRLA
ANMLGVSRVAEACKVRGLYP

Sequences:

>Translated_420_residues
MLATATTPMNAYDVALENFDLAANALGLDDSIRAMIKYPERILSVSVPVRMDTGKIVRFEGYRVQHSTMRGPAKGGIRFH
PNVTMDEVKALATWMTWKCAVVNIPYGGGKGGVTCNPKELSMGELERMTRRYASSILPIIGPEKDIPAPDVYTTPQIMAW
IMDTYSMNKGYPVHGVVTGKPLSIGGSLGRNEATARGVFYTTMSSCEHLGIQLAGSRVVVQGFGNAGAIAADLFHGAGAK
VLAVSDTSGCIFNKNGLHIPAVVAYKARTGRLEGFPEATRITPAELLALECEILVPAALENAITEENAHTIHAKIISEAA
NGPVTPEADRILGSKGIFLIPDILCNAGGVTVSYFEWVQDENHLFWDEQDVNAKLEKIMKRSFADVLKIHLDKKVDMRLA
ANMLGVSRVAEACKVRGLYP
>Mature_420_residues
MLATATTPMNAYDVALENFDLAANALGLDDSIRAMIKYPERILSVSVPVRMDTGKIVRFEGYRVQHSTMRGPAKGGIRFH
PNVTMDEVKALATWMTWKCAVVNIPYGGGKGGVTCNPKELSMGELERMTRRYASSILPIIGPEKDIPAPDVYTTPQIMAW
IMDTYSMNKGYPVHGVVTGKPLSIGGSLGRNEATARGVFYTTMSSCEHLGIQLAGSRVVVQGFGNAGAIAADLFHGAGAK
VLAVSDTSGCIFNKNGLHIPAVVAYKARTGRLEGFPEATRITPAELLALECEILVPAALENAITEENAHTIHAKIISEAA
NGPVTPEADRILGSKGIFLIPDILCNAGGVTVSYFEWVQDENHLFWDEQDVNAKLEKIMKRSFADVLKIHLDKKVDMRLA
ANMLGVSRVAEACKVRGLYP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=375, Percent_Identity=44, Blast_Score=298, Evalue=8e-81,
Organism=Homo sapiens, GI4885281, Length=375, Percent_Identity=44, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1788059, Length=401, Percent_Identity=32.4189526184539, Blast_Score=180, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17544676, Length=368, Percent_Identity=41.8478260869565, Blast_Score=283, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6319256, Length=383, Percent_Identity=29.7650130548303, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324951, Length=410, Percent_Identity=28.0487804878049, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45553475, Length=370, Percent_Identity=41.6216216216216, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI45549226, Length=367, Percent_Identity=41.41689373297, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24649283, Length=351, Percent_Identity=41.8803418803419, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649460, Length=312, Percent_Identity=40.3846153846154, Blast_Score=225, Evalue=5e-59,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45433; Mature: 45433

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATATTPMNAYDVALENFDLAANALGLDDSIRAMIKYPERILSVSVPVRMDTGKIVRFE
CCCCCCCCCCHHHHEECCCCHHHHCCCCCHHHHHHHHCCHHHEEEECEEEECCCCEEEEE
GYRVQHSTMRGPAKGGIRFHPNVTMDEVKALATWMTWKCAVVNIPYGGGKGGVTCNPKEL
CEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCEEECHHCC
SMGELERMTRRYASSILPIIGPEKDIPAPDVYTTPQIMAWIMDTYSMNKGYPVHGVVTGK
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECC
PLSIGGSLGRNEATARGVFYTTMSSCEHLGIQLAGSRVVVQGFGNAGAIAADLFHGAGAK
CEECCCCCCCCCCHHCEEEEEEHHHHHHCCEEEECCEEEEEECCCCCCHHHHHHCCCCCE
VLAVSDTSGCIFNKNGLHIPAVVAYKARTGRLEGFPEATRITPAELLALECEILVPAALE
EEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEEEEEEEEHHHHH
NAITEENAHTIHAKIISEAANGPVTPEADRILGSKGIFLIPDILCNAGGVTVSYFEWVQD
HHHCCCCCCEEHHHHHHHHCCCCCCCCHHHHHCCCCCEEEHHHHHCCCCCEEEEEEHHCC
ENHLFWDEQDVNAKLEKIMKRSFADVLKIHLDKKVDMRLAANMLGVSRVAEACKVRGLYP
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLATATTPMNAYDVALENFDLAANALGLDDSIRAMIKYPERILSVSVPVRMDTGKIVRFE
CCCCCCCCCCHHHHEECCCCHHHHCCCCCHHHHHHHHCCHHHEEEECEEEECCCCEEEEE
GYRVQHSTMRGPAKGGIRFHPNVTMDEVKALATWMTWKCAVVNIPYGGGKGGVTCNPKEL
CEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHEEEEEEEECCCCCCCCCEEECHHCC
SMGELERMTRRYASSILPIIGPEKDIPAPDVYTTPQIMAWIMDTYSMNKGYPVHGVVTGK
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECC
PLSIGGSLGRNEATARGVFYTTMSSCEHLGIQLAGSRVVVQGFGNAGAIAADLFHGAGAK
CEECCCCCCCCCCHHCEEEEEEHHHHHHCCEEEECCEEEEEECCCCCCHHHHHHCCCCCE
VLAVSDTSGCIFNKNGLHIPAVVAYKARTGRLEGFPEATRITPAELLALECEILVPAALE
EEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEEEEEEEEHHHHH
NAITEENAHTIHAKIISEAANGPVTPEADRILGSKGIFLIPDILCNAGGVTVSYFEWVQD
HHHCCCCCCEEHHHHHHHHCCCCCCCCHHHHHCCCCCEEEHHHHHCCCCCEEEEEEHHCC
ENHLFWDEQDVNAKLEKIMKRSFADVLKIHLDKKVDMRLAANMLGVSRVAEACKVRGLYP
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]