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The map label for this gene is pdxT [H]

Identifier: 116516892

GI number: 116516892

Start: 1315317

End: 1315898

Strand: Reverse

Name: pdxT [H]

Synonym: SPD_1296

Alternate gene names: 116516892

Gene position: 1315898-1315317 (Counterclockwise)

Preceding gene: 116515752

Following gene: 116515345

Centisome position: 64.31

GC content: 42.27

Gene sequence:

>582_bases
ATGAAAATCGGAATATTGGCCTTGCAAGGGGCCTTTGCAGAACATGCAAAAGTGCTAGATCAATTAGGTGTCGAGAGTGT
AGAACTCAGAAATCTAGATGATTTTCAGCAAGATCAGAGTGACTTGTCGGGTTTGATTTTGCCTGGTGGTGAGTCTACAA
CCATGGGCAAGCTCTTACGTGACCAGAACATGCTACTTCCCATACGAGAAGCCATTCTATCTGGCTTACCAGTGTTTGGG
ACCTGTGCGGGCTTAATTTTGCTGGCTAAGGAAATCACTTCTCAGAAAGAGAGTCATCTAGGAACTATGGATATGGTGGT
CGAGCGTAATGCTTATGGGCGCCAATTAGGAAGTTTCTACACGGAAGCAGAATGTAAGGGAGTTGGCAAGATTCCAATGA
CCTTTATCCGTGGTCCGATTATCAGTAGTGTTGGTGAGGGTGTAGAAATTTTAGCAATAGTGAACAATCAAATTGTTGCA
GCCCAAGAAAAAAATATGTTGGTAAGTTCTTTTCATCCAGAATTGACTGATGATGTGCGCTTGCACCAGTACTTTATCAA
TATGTGTAAAGAAAAAAGTTGA

Upstream 100 bases:

>100_bases
TCCTCAAATCCTAGCTCAAATCTCTGAAGATTTAGGAGAAGCCATGGTTGGTATTAATGAAAATGAAATCCAAATTCTCA
TGGCTGAACGAGGAAAATAG

Downstream 100 bases:

>100_bases
GATTGAATTTCTCAACTTTTTTACATGTAATAAACAATAGCGATGTATTGGAGTGCGGACGCAGCTAGGATAAAGAGATG
CCAAATCATGTGGAAATAAG

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFG
TCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILAIVNNQIVA
AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS

Sequences:

>Translated_193_residues
MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFG
TCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILAIVNNQIVA
AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS
>Mature_193_residues
MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFG
TCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILAIVNNQIVA
AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS [H]

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=205, Percent_Identity=36.0975609756098, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321048, Length=210, Percent_Identity=34.2857142857143, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=33.8095238095238, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002161
- InterPro:   IPR021196 [H]

Pfam domain/function: PF01174 SNO [H]

EC number: NA

Molecular weight: Translated: 21107; Mature: 21107

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01236 PDXT_SNO_1 ; PS51130 PDXT_SNO_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLR
CCEEEEEHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCEEEECCCCCHHHHHHHH
DQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFY
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHH
TEAECKGVGKIPMTFIRGPIISSVGEGVEILAIVNNQIVAAQEKNMLVSSFHPELTDDVR
HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEEEHHCCHHHHHCCCCCCHHHH
LHQYFINMCKEKS
HHHHHHHHHHCCC
>Mature Secondary Structure
MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLR
CCEEEEEHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHCCEEEECCCCCHHHHHHHH
DQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFY
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHH
TEAECKGVGKIPMTFIRGPIISSVGEGVEILAIVNNQIVAAQEKNMLVSSFHPELTDDVR
HHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCEEEEEHHCCHHHHHCCCCCCHHHH
LHQYFINMCKEKS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA