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The map label for this gene is dus1 [H]

Identifier: 116516462

GI number: 116516462

Start: 1993858

End: 1994814

Strand: Direct

Name: dus1 [H]

Synonym: SPD_2016

Alternate gene names: 116516462

Gene position: 1993858-1994814 (Clockwise)

Preceding gene: 116515969

Following gene: 116515439

Centisome position: 97.45

GC content: 48.9

Gene sequence:

>957_bases
ATGATTGGCAATATTGAGATTCCCAATCGTACCGTTTTAGCGCCTATGGCTGGCGTGACCAACTCAGCCTTTCGTACCAT
CGCAAAAGAGCTCGGAGCTGGACTCGTTGTAATGGAAATGGTCTCTGACAAGGGAATCCAATACAACAACGAAAAAACCC
TGCATATGCTTCATATCGATGAGGGCGAAAACCCTGTCTCTATCCAACTTTTTGGTAGCGATGAAGACAGCCTAGCACGC
GCAGCAGAATTCATCCAAGAAAACACCAAGACCGATATCGTCGATATCAACATGGGCTGCCCTGTCAACAAAATCGTGAA
GAACGAAGCTGGAGCTATGTGGCTCAAGGATCCTGACAAGATCTACTCTATCATCAACAAGGTCCAGTCTGTCCTTGATA
TCCCACTTACTGTCAAAATGCGTACCGGCTGGGCGGACCCATCTCTGGCAGTAGAAAATGCCCTCGCTGCTGAGGCTGCA
GGTGTTTCTGCCCTCGCCATGCATGGCCGTACCCGTGAACAAATGTATACTGGCCACGCAGACCTTGAGACCCTTTACAA
GGTTGCCCAAGCTCTAACCAAGATTCCATTCATCGCCAACGGTGATATCCGTACTGTCCAAGAAGCCAAGCAACGCATCG
AAGAAGTTGGTGCTGACGCAGTCATGATTGGCCGAGCTGCCATGGGAAATCCTTACCTCTTCAACCAAATCAACCATTAC
TTTGAAACAGGAGAAATCCTACCTGATTTGACCTTTGAAGACAAGATGAAGATCGCCTACGAACACTTGAAACGATTGAT
TAACCTCAAAGGAGAAAACGTCGCAGTTCGTGAATTCCGCGGCCTCGCTCCTCACTATCTCCGTGGAACATCTGGCGCTG
CCAAACTCCGTGGAGCCATTTCGCAAGCTAGCACCCTAGCAGAGATTGAAGCCCTCTTGCAATTGGAGAAGGCTTAA

Upstream 100 bases:

>100_bases
CACGGGGCAGAAATCACTTGTCAATTCTGCCAAACTACTTACAACTTTGATGAAAAGGACCTGGAGGAACTCATTCGTGA
CAAATCTTAATACACCTTTT

Downstream 100 bases:

>100_bases
TAGTTTAAAACCCGTAACTCTCTTAAAGAGTCTCTTGAATGCCGCCATTCTTTCCTCATCAAAAAAACTCCGTAGTCTCT
AGCGCTATAAATCCGGCTAG

Product: TIM-barrel protein, nifR3 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLAR
AAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAA
GVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHY
FETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA

Sequences:

>Translated_318_residues
MIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLAR
AAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAA
GVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHY
FETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA
>Mature_318_residues
MIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLAR
AAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAA
GVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHY
FETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=256, Percent_Identity=32.03125, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI239788462, Length=237, Percent_Identity=32.0675105485232, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI31742496, Length=296, Percent_Identity=26.3513513513513, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=32.1285140562249, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40807366, Length=289, Percent_Identity=26.9896193771626, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1789660, Length=316, Percent_Identity=39.5569620253165, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=34.5679012345679, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI145693211, Length=255, Percent_Identity=24.3137254901961, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=235, Percent_Identity=30.2127659574468, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=28.0334728033473, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=250, Percent_Identity=26.8, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=26.3157894736842, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=244, Percent_Identity=30.7377049180328, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=261, Percent_Identity=26.0536398467433, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=24.0174672489083, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=31.7796610169492, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=29.8701298701299, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=29.8701298701299, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45549423, Length=246, Percent_Identity=31.7073170731707, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.3842794759825, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34863; Mature: 34863

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00634 RIBOSOMAL_L30 ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHID
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCEECCCCEEEEEEEC
EGENPVSIQLFGSDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDK
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCEEEECCHHH
IYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAAGVSALAMHGRTREQMYTGHA
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCC
DLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHY
CHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHH
FETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAI
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHCCCCCHHHHHHHH
SQASTLAEIEALLQLEKA
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLVVMEMVSDKGIQYNNEKTLHMLHID
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEEEEHHHCCCCEECCCCEEEEEEEC
EGENPVSIQLFGSDEDSLARAAEFIQENTKTDIVDINMGCPVNKIVKNEAGAMWLKDPDK
CCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHCCCCEEEECCHHH
IYSIINKVQSVLDIPLTVKMRTGWADPSLAVENALAAEAAGVSALAMHGRTREQMYTGHA
HHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCC
DLETLYKVAQALTKIPFIANGDIRTVQEAKQRIEEVGADAVMIGRAAMGNPYLFNQINHY
CHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHH
FETGEILPDLTFEDKMKIAYEHLKRLINLKGENVAVREFRGLAPHYLRGTSGAAKLRGAI
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCHHHCCCCCHHHHHHHH
SQASTLAEIEALLQLEKA
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]