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The map label for this gene is rsmF [H]

Identifier: 116516379

GI number: 116516379

Start: 1264611

End: 1265915

Strand: Reverse

Name: rsmF [H]

Synonym: SPD_1233

Alternate gene names: 116516379

Gene position: 1265915-1264611 (Counterclockwise)

Preceding gene: 116515379

Following gene: 116517085

Centisome position: 61.87

GC content: 45.52

Gene sequence:

>1305_bases
ATGCAATTTCCAGAAGGATTTGTTGAAAAATATAAAGAGATACTAGGAGATGAGGCAAGAGATTTTCTTGCCTCTTTTGA
GGAGGAAGCGGTTTCGGCCTTTCGGGTCAATCCCTTAAAAGAAGAGCAACTTTCCTTTTCTGATGCCATTACTCAAACCC
CTTGGGGCCACTATGGGAAGGTTTCAGGGAAATCGCCTGAGCATGCTACAGGTTTAGTTTATTCGCAAGAACCTGCTGCT
CAAATGGTGGCTCAAGTAGCCCAACCCAGTCCTGGTATGAAGGTCTTGGACTTGGCTGCTGCACCGGGGGGCAAATCAAC
TCAACTGGCAGCCTATCTAGCAGGTGAGGGGCTACTTGTTTCCAATGAAATTTCAAGCAAACGGGCTAAGATTTTGGTTG
AAAACATGGAGCGTTTTGGAGCGACAAATGTCGTGGTGACCAATGAATCTGCCGACCGCTTGGTCAAGGTCTTTAAAGGC
TATTTTGACTTAATCGTTCTTGATGCCCCTTGCTCTGGTGAAGGAATGTTTCGTAAGCAACCTGATGCTATGGACTATTG
GAGCTTAGATTATCCGAGTCAATGTGCTAGCTTGCAAAGAGAAATTCTGGAGGATGCAGTGACCATGTTAGCTGAAGGTG
GTCGTCTGGTTTATTCGACCTGTACCTGGGCACCCGAGGAAAACGAAGAGATTGTCAATTGGTTGCTGGAAGAGTATGAT
TTTGACTTGTTGCCAGTAGAGCATATCAATGGAATGGTAGCTGGTATTGACCTGCCAGAAACGGCTCGGATGTACCCTCA
TCAGTTTAAGGGAGAGGGTCAGTTTGTTGCCCATCTACAGTTTAAGGGTAACAATCCAGCTCCTAAATTTAAGGCAAGTA
AGAGTAACCTCAGCCGTGAACAAGTTGCCTTGTGGCAGGAATTTGCCCAAAACCATTTGAAGGTCAATCTACCGGGTATC
TTGCAGACTTTTGGAGACCAGCTTTATCTCTTGCCAGAACTTTTGCCAGATTTAGGGAAGCTCAAGATTGCTCGCAATGG
ACTGCATCTGGGCACTTTTAAGAAGAAACGCTTTGAGCCCAGTTTCGCTCTTGGTTTAGCCTTGAAACCGAGTCAGGTCG
AACAGTCGGTTGAAATTGGCCAAGAAGCGTTTGTCAAGTATGCGGCTGGAGAAACCGTTCAGCTGGCAGAAAGTCTGCCA
AATGGTTGGTACCAAGTTTTGGTTAAAGGCAATGGCTTGGGCTTTGCAAAGGTTACTGGAAATGTTTTGAAAAATTATTT
TCCAAAAGGCCTCAGATTCAAATGA

Upstream 100 bases:

>100_bases
TGAAGCTCCTGATTTTCAAACCAGACAGCCGGTCATGATGGTGCCTCTTGAGATGCAGGAGGAAATTCAGTCCTATATTT
ACGAAAGGAAAAGAACTTAA

Downstream 100 bases:

>100_bases
AAATGCTAGTCAAATATCTTATTCTATGTTATAGTGAAAGTATGGATTTTTTTAGTAGGATGATTCTTGATATCTTGATT
GCCATCACATGTAGTTTTAC

Product: NOL1/NOP2/sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQLSFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAA
QMVAQVAQPSPGMKVLDLAAAPGGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVYSTCTWAPEENEEIVNWLLEEYD
FDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFVAHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGI
LQTFGDQLYLLPELLPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGETVQLAESLP
NGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK

Sequences:

>Translated_434_residues
MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQLSFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAA
QMVAQVAQPSPGMKVLDLAAAPGGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVYSTCTWAPEENEEIVNWLLEEYD
FDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFVAHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGI
LQTFGDQLYLLPELLPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGETVQLAESLP
NGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK
>Mature_434_residues
MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQLSFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAA
QMVAQVAQPSPGMKVLDLAAAPGGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVYSTCTWAPEENEEIVNWLLEEYD
FDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFVAHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGI
LQTFGDQLYLLPELLPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGETVQLAESLP
NGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=307, Percent_Identity=28.3387622149837, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI76150623, Length=307, Percent_Identity=28.3387622149837, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI301336155, Length=262, Percent_Identity=29.3893129770992, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI39995082, Length=264, Percent_Identity=30.3030303030303, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI32698918, Length=179, Percent_Identity=32.9608938547486, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI11545785, Length=183, Percent_Identity=32.2404371584699, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI270288818, Length=215, Percent_Identity=29.3023255813954, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI8922322, Length=215, Percent_Identity=29.3023255813954, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI23199998, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI270288816, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI87081985, Length=461, Percent_Identity=30.8026030368764, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI2367212, Length=177, Percent_Identity=35.5932203389831, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=297, Percent_Identity=29.6296296296296, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25143471, Length=183, Percent_Identity=31.1475409836066, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=297, Percent_Identity=29.6296296296296, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319447, Length=181, Percent_Identity=32.5966850828729, Blast_Score=101, Evalue=2e-22,
Organism=Drosophila melanogaster, GI22024126, Length=280, Percent_Identity=29.2857142857143, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI21355201, Length=191, Percent_Identity=32.4607329842932, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356513, Length=173, Percent_Identity=30.635838150289, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24668781, Length=172, Percent_Identity=30.8139534883721, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 48050; Mature: 48050

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQLSFSDAITQTPWGHYGK
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
VSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAPGGKSTQLAAYLAGEGLLV
CCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCCEEE
SNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKGYFDLIVLDAPCSGEGMFRKQ
ECCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCC
PDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVYSTCTWAPEENEEIVNWLLEEYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCC
FDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFVAHLQFKGNNPAPKFKASKSNLSRE
CCCEEHHHHCCEEEECCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHCCCHH
QVALWQEFAQNHLKVNLPGILQTFGDQLYLLPELLPDLGKLKIARNGLHLGTFKKKRFEP
HHHHHHHHHHCCEEEECCHHHHHHCCCEEEHHHHCCCHHHEEECCCCCEECCCHHHHCCC
SFALGLALKPSQVEQSVEIGQEAFVKYAAGETVQLAESLPNGWYQVLVKGNGLGFAKVTG
CCEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCEEHHHHH
NVLKNYFPKGLRFK
HHHHHHCCCCCCCC
>Mature Secondary Structure
MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQLSFSDAITQTPWGHYGK
CCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
VSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAPGGKSTQLAAYLAGEGLLV
CCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCCEEE
SNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKGYFDLIVLDAPCSGEGMFRKQ
ECCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCC
PDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVYSTCTWAPEENEEIVNWLLEEYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCC
FDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFVAHLQFKGNNPAPKFKASKSNLSRE
CCCEEHHHHCCEEEECCCCHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHCCCHH
QVALWQEFAQNHLKVNLPGILQTFGDQLYLLPELLPDLGKLKIARNGLHLGTFKKKRFEP
HHHHHHHHHHCCEEEECCHHHHHHCCCEEEHHHHCCCHHHEEECCCCCEECCCHHHHCCC
SFALGLALKPSQVEQSVEIGQEAFVKYAAGETVQLAESLPNGWYQVLVKGNGLGFAKVTG
CCEEEEEECHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECCCCEEHHHHH
NVLKNYFPKGLRFK
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]