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The map label for this gene is murE

Identifier: 116516282

GI number: 116516282

Start: 1374930

End: 1376375

Strand: Direct

Name: murE

Synonym: SPD_1359

Alternate gene names: 116516282

Gene position: 1374930-1376375 (Clockwise)

Preceding gene: 116515433

Following gene: 116517197

Centisome position: 67.2

GC content: 42.67

Gene sequence:

>1446_bases
ATGATTAAGATTGAAACCGTATTAGATATTTTAAAGAAAGATGGCCTTTTTCGCGAAATTATTGACCAAGGTCATTACCA
CTACAACTACAGCAAAGTTATTTTTGATAGCATCAGCTACGACAGCCGAAAAGTAACAGAAGACACTCTTTTTTTTGCAA
AAGGCGCTGCCTTTAAAAAAGAATACCTTCTTTCTGCTATAACACAAGGTTTAGCTTGGTATGTAGCTGAAAAGGACTAC
GAAGTCGATATCCCTGTCATCATTGTGAACGATATAAAGAAAGCCATGAGTTTGATTGCCATGGAGTTCTATGGTAATCC
ACAAGAGAAACTCAAACTCCTTGCCTTTACTGGTACTAAGGGTAAGACAACAGCAACCTATTTCGCCTATAACATCTTAT
CTCAAGGGCATAGACCTGCTATGTTGTCGACCATGAACACAACTCTTGATGGCGAGACTTTCTTTAAGTCAGCGTTGACA
ACCCCTGAGAGTATTGACCTCTTTGACATGATGAATCAGGCTGTGCAAAATGACCGTACCCACCTCATCATGGAAGTCTC
CAGTCAAGCCTATCTAGTCCATCGAGTCTATGGACTGACCTTTGATGTAGGAGTCTTTCTTAACATCACTCCTGACCATA
TCGGCCCGATTGAACACCCTAGCTTTGAAGACTATTTCTACCACAAGCGTCTCTTGATGGAAAATAGCCGAGCAGTCATC
ATTAACAGTGACATGGACCACTTCTCAGTCTTGAAAGAACAGGTTGAAGATCAAGACCATGATTTCTATGGTAGCCAATT
TGATAACCAAATCGAGAATTCCAAAGCCTTTAGCTTTTCAGCTACGGGTAAACTCGCTGGAGATTATGATATCCAACTCA
TTGGCAACTTCAACCAAGAAAATGCAGTTGCTGCTGGACTTGCTTGTCTCCGTCTCGGAGCAAGTCTTGAGGACATCAAA
AAAGGCATCGCTGCAACCCGCGTTCCTGGTCGTATGGAAGTCCTCACTCAGAAAAATGGAGCCAAGGTCTTCATCGACTA
TGCCCACAATGGGGATAGTCTGAAAAAACTCATCAATGTGGTTGAAACTCATCAAACCGGAAAGATTGCTCTGGTTCTGG
GATCAACAGGAAACAAGGGAGAAAGTCGTCGTAAGGACTTTGGCCTCCTCCTCAATCAACACCCTGAGATTCAAGTCTTT
CTGACTGCTGATGACCCTAACTATGAAGACCCAATGGCCATTGCAGATGAAATTAGTAGCTACATCAATCATCCTGTTGA
AAAGATTGCGGATCGCCAAGAAGCCATCAAGGCGGCAATGGCTATCACAAATCACGAATTAGATGCAGTTATTATTGCGG
GTAAGGGAGCCGATTGTTACCAAATCATCCAGGGCAAGAAAGAATCCTACCCAGGAGATACAGCCGTCGCAGAAAATTAT
TTATAA

Upstream 100 bases:

>100_bases
CCTCATTCAATTTTGATAACTAGGAAAAACTCCTTATCTTCCATTATAAACTTTTCCTTGTTACTTGTAAATTTACGACT
TTCGGTTTACAATAGAAAGT

Downstream 100 bases:

>100_bases
GAATAGAAAAAATCAAGGGAAAATGAAACCCTTGATTTTTTCCTATTATTTAGTAAAAACGTTTTTCAAACCTTCAACGG
CACCTTCTACAGCACCTTTT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--L-lysine ligase

Products: NA

Alternate protein names: L-lysine-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:L-Lys ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRKVTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDY
EVDIPVIIVNDIKKAMSLIAMEFYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIEHPSFEDYFYHKRLLMENSRAVI
INSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKAFSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIK
KGIAATRVPGRMEVLTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLNQHPEIQVF
LTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAVIIAGKGADCYQIIQGKKESYPGDTAVAENY
L

Sequences:

>Translated_481_residues
MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRKVTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDY
EVDIPVIIVNDIKKAMSLIAMEFYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIEHPSFEDYFYHKRLLMENSRAVI
INSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKAFSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIK
KGIAATRVPGRMEVLTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLNQHPEIQVF
LTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAVIIAGKGADCYQIIQGKKESYPGDTAVAENY
L
>Mature_481_residues
MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRKVTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDY
EVDIPVIIVNDIKKAMSLIAMEFYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIEHPSFEDYFYHKRLLMENSRAVI
INSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKAFSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIK
KGIAATRVPGRMEVLTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLNQHPEIQVF
LTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAVIIAGKGADCYQIIQGKKESYPGDTAVAENY
L

Specific function: Catalyzes the addition of L-lysine to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=477, Percent_Identity=29.3501048218029, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_STRP2 (Q04JL8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816820.1
- ProteinModelPortal:   Q04JL8
- SMR:   Q04JL8
- STRING:   Q04JL8
- EnsemblBacteria:   EBSTRT00000019625
- GeneID:   4442610
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1359
- eggNOG:   COG0769
- GeneTree:   EBGT00050000027196
- HOGENOM:   HBG351099
- OMA:   IGTIANY
- ProtClustDB:   PRK14022
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.7

Molecular weight: Translated: 53829; Mature: 53829

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: BINDING 42-42 BINDING 187-187 BINDING 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRKVTEDTLFFAKGAAFKK
CEEHHHHHHHHHCCCHHHHHHHCCCEEEEHHHEEEECCCCCCCCCCHHHHEEECCCHHHH
EYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAMEFYGNPQEKLKLLAFTGTK
HHHHHHHHHHHEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCC
GKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALTTPESIDLFDMMNQAVQNDRT
CCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
HLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIEHPSFEDYFYHKRLLMENSRAVI
EEEEEECCCEEEEHHHHCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
INSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKAFSFSATGKLAGDYDIQLIGNFNQE
EECCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCC
NAVAAGLACLRLGASLEDIKKGIAATRVPGRMEVLTQKNGAKVFIDYAHNGDSLKKLINV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHH
VETHQTGKIALVLGSTGNKGESRRKDFGLLLNQHPEIQVFLTADDPNYEDPMAIADEISS
HHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
YINHPVEKIADRQEAIKAAMAITNHELDAVIIAGKGADCYQIIQGKKESYPGDTAVAENY
HHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCHHHCCC
L
C
>Mature Secondary Structure
MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRKVTEDTLFFAKGAAFKK
CEEHHHHHHHHHCCCHHHHHHHCCCEEEEHHHEEEECCCCCCCCCCHHHHEEECCCHHHH
EYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAMEFYGNPQEKLKLLAFTGTK
HHHHHHHHHHHEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHEEEEEEECCC
GKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALTTPESIDLFDMMNQAVQNDRT
CCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
HLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIEHPSFEDYFYHKRLLMENSRAVI
EEEEEECCCEEEEHHHHCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
INSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKAFSFSATGKLAGDYDIQLIGNFNQE
EECCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCEEEEEEECCCCC
NAVAAGLACLRLGASLEDIKKGIAATRVPGRMEVLTQKNGAKVFIDYAHNGDSLKKLINV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHH
VETHQTGKIALVLGSTGNKGESRRKDFGLLLNQHPEIQVFLTADDPNYEDPMAIADEISS
HHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHH
YINHPVEKIADRQEAIKAAMAITNHELDAVIIAGKGADCYQIIQGKKESYPGDTAVAENY
HHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCHHHCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA