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The map label for this gene is glyS

Identifier: 116516232

GI number: 116516232

Start: 1321865

End: 1323901

Strand: Reverse

Name: glyS

Synonym: SPD_1304

Alternate gene names: 116516232

Gene position: 1323901-1321865 (Counterclockwise)

Preceding gene: 116516637

Following gene: 116517108

Centisome position: 64.7

GC content: 41.92

Gene sequence:

>2037_bases
ATGACAAAAAACTTATTAGTAGAACTCGGTCTTGAAGAATTACCAGCCTATGTTGTCACACCAAGTGAAAAACAACTAGG
CGAAAAAATGGCAGCCTTCCTCAAGGAAAACCGCCTGTCTTTTGAAGCCATTCAAACCTTCTCAACACCACGTCGTTTGG
CTGTTCGTGTGACTGGTTTATCAGACAAACAGTCTGATTTGACAGAAGATTTCAAGGGACCAGCAAAGAAAATTGCCTTG
GATAGTGATGGAAACTTCACCAAAGCAGCTCAAGGATTTGTCCGTGGAAAAGGCTTGACTGTTGAAGATATCGAATTCCG
TGAAATCAAGGGTGAAGAATATGTCTATGTCACTAAGGAAGAAGTTGGTCAAGCAGTTGAAGCCATTGTTCCTGGTGTTG
TAGATGTCTTGAAGTCATTGACTTTCCCTGTCAGCATGCACTGGGCTGGAAATAGCTTTGAATACATCCGCCCTGTTCAC
ACTTTAACTGTTCTCTTGGATGAGCAAGAGTTTGACTTGGATTTCCTTGATATCAAGGGAGGTCGTGTGAGCCGTGGCCA
TCGTTTCTTGGGGAAAGAAACCAAAATTCAGTCAGCATTGAGCTATGAAGAAGACCTTCGTAAGCAGTTTGTAATCGCAG
ATCCATGTGAACGTGAGCAAATGATTGTTGACCAAATCAAGAAAATTGAGGCAAAACATGGTGTACGTATCGAAATTGAT
GCGGATTTGCTGAATGAAGTCTTGAATTTGGTTGAATACCCAACTGCCTTCATGGGAAGTTTTGATGCTAAATACCTTGA
AGTTCCAGAAGAAGTCTTGGTGACTTCTATGAAGGAACACCAGCGTTACTTTGTTGTTCGTGATCAAGATGGAAAACTCT
TGCCAAACTTCATTTCTGTTCGTAACGGAAACGCAGAGCGTTTGAAAAATGTCATCAAAGGAAATGAAAAAGTCTTGGTA
GCCCGCTTGGAAGACGGAGAATTCTTCTGGCGTGAAGACCAAAAATTGGTGATTTCAGATCTTGTTGAAAAATTAAACAA
TGTCACCTTCCATGAGAAGATTGGTTCCCTTCGTGAACACATGATTCGTACGGGTCAAATCACTGTACTTTTGGCAGAAA
AAGCTGGTTTGTCAGTGGATGAAACAGTTGACCTTGCTCGTGCAGCAGCCATTTACAAGTTTGACTTGTTGACAGGTATG
GTTGGTGAATTTGACGAACTCCAAGGAATTATGGGTGAAAAATACACCCTTCTTGCTGGTGAAACTCCAGCGGTGGCAGC
TGCTATTCGTGAACACTACATGCCTACATCAGCTGAAGGAGAACTTCCAGAGAGCAAGGTCGGTGCAGTTCTAGCCATTG
CAGACAAATTGGATACGATTTTGAGTTTCTTCTCAGTAGGATTGATTCCATCAGGTTCTAATGACCCTTATGCCCTTCGT
CGTGCAACTCAAGGTGTGGTTCGTATCTTGGATGCCTTTGGTTGGCACATTGCTATGGATGAGCTGATTGATAGCCTTTA
TGCATTGAAATTTGACAGTTTGACTTATGAAAATAAAGCAGAGGTTATGGACTTTATCAAGGCTCGTGTTGATAAGATGA
TGGGCTCTACTCCAAAAGATATCAAGGAAGCAGTTCTTGCAGGTTCAAACTTTGTTGTGGCAGATATGTTGGAAGCAGCA
AGTGCTCTCGTAGAAGTAAGCAAGGAAGAAGATTTTAAACCATCTGTTGAATCACTTTCTCGTGCCTTTAACCTGGCTGA
GAAGGCAGAAGGGGTTGCCACAGTTGATTCAGCACTATTTGAGAATGACCAAGAAAAAGCTTTGGCAGAAGCAGTAGAAA
CACTCGTTTTATCAGGACCTGCAAGTCAGCAATTGAAACAACTCTTTGCGCTTAGCCCAGTCATTGATGCTTTCTTTGAA
AATACTATGGTAATGGCTGAAGATCAGGCTGTCCGTCAAAATCGTTTGGCAATCTTGTCACAACTAACCAAGAAAGCAGC
TAAGTTTGCTTGTTTTAACCAAATTAACACTAAATAA

Upstream 100 bases:

>100_bases
AGCATTGGATTTCCATACAGACTCACAGAGTTAGTTAGCGACGTCCCCAGTACCTAAGGTGCATATCAAAAAAGATTGAA
GAAAATGTAAAGGAAAAAAC

Downstream 100 bases:

>100_bases
AATTTGATAAACGGACTTTATCTTATTACAGAGGAGAAGAAATGGATCCGAAAAAAATTGCTCGTATCAATGAGCTTGCT
AAAAAGAAAAAAACAGAAGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAIQTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIAL
DSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
TLTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKKIEAKHGVRIEID
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLV
ARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIYKFDLLTGM
VGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAA
SALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFE
NTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK

Sequences:

>Translated_678_residues
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAIQTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIAL
DSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
TLTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKKIEAKHGVRIEID
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLV
ARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIYKFDLLTGM
VGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAA
SALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFE
NTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK
>Mature_677_residues
TKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAIQTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALD
SDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVHT
LTVLLDEQEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKKIEAKHGVRIEIDA
DLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVA
RLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIYKFDLLTGMV
GEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALRR
ATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAAS
ALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFEN
TMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=676, Percent_Identity=35.5029585798817, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_STRP2 (Q04JM8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816770.1
- ProteinModelPortal:   Q04JM8
- STRING:   Q04JM8
- EnsemblBacteria:   EBSTRT00000020669
- GeneID:   4441512
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1304
- eggNOG:   COG0751
- GeneTree:   EBGT00050000028086
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- InterPro:   IPR003607
- PRINTS:   PR01045
- SMART:   SM00471
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75411; Mature: 75280

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAIQTFSTPRRLAVRVTGL
CCCCCEEECCHHHCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECC
SDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKE
CCCHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEHH
EVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVHTLTVLLDEQEFDLDFLDIKG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEEEECCC
GRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKKIEAKHGVRIEID
CCCCCCCHHHCCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEC
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISV
HHHHHHHHHHHHCCHHHHCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHC
RNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
MIRTGQITVLLAEKAGLSVDETVDLARAAAIYKFDLLTGMVGEFDELQGIMGEKYTLLAG
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
ETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IKEAVLAGSNFVVADMLEAASALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
ENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
QLTKKAAKFACFNQINTK
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAIQTFSTPRRLAVRVTGL
CCCCEEECCHHHCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEECC
SDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVEDIEFREIKGEEYVYVTKE
CCCHHHHHHHHCCCHHEEEECCCCCHHHHHHHHHCCCCCEEECCHHHCCCCCCEEEEEHH
EVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVHTLTVLLDEQEFDLDFLDIKG
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEEEECCC
GRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCEREQMIVDQIKKIEAKHGVRIEID
CCCCCCCHHHCCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHCCCEEEEC
ADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTSMKEHQRYFVVRDQDGKLLPNFISV
HHHHHHHHHHHHCCHHHHCCCCCHHHHCCHHHHHHHHHHCCEEEEEECCCCCCCCHHHHC
RNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQKLVISDLVEKLNNVTFHEKIGSLREH
CCCCHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
MIRTGQITVLLAEKAGLSVDETVDLARAAAIYKFDLLTGMVGEFDELQGIMGEKYTLLAG
HHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEC
ETPAVAAAIREHYMPTSAEGELPESKVGAVLAIADKLDTILSFFSVGLIPSGSNDPYALR
CCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RATQGVVRILDAFGWHIAMDELIDSLYALKFDSLTYENKAEVMDFIKARVDKMMGSTPKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHH
IKEAVLAGSNFVVADMLEAASALVEVSKEEDFKPSVESLSRAFNLAEKAEGVATVDSALF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
ENDQEKALAEAVETLVLSGPASQQLKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILS
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHH
QLTKKAAKFACFNQINTK
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA