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The map label for this gene is rnjA [H]

Identifier: 116516160

GI number: 116516160

Start: 134636

End: 136315

Strand: Reverse

Name: rnjA [H]

Synonym: SPD_0130

Alternate gene names: 116516160

Gene position: 136315-134636 (Counterclockwise)

Preceding gene: 116515572

Following gene: 116515314

Centisome position: 6.66

GC content: 46.49

Gene sequence:

>1680_bases
ATGGCCTACACTCTTAAACCTGAAGAAGTCGGCGTTTTTGCCATCGGTGGTCTAGGAGAAATCGGGAAAAACACTTACGG
AATTGAATACCAAGACGAGATTATCATCGTCGATGCTGGGATTAAATTCCCAGAAGATGACTTGCTTGGTATCGACTATG
TCATTCCTGACTACTCTTACATCGTAGACAATATCGACCGCGTCAAGGCTGTTTTAATCACACACGGACACGAGGACCAC
ATTGGTGGGATTCCGTTCCTACTCAAGCAAGCAAATGTCCCTATTTATGCTGGACCGCTTGCCTTGGCTTTGATCCGTGG
GAAACTCGAAGAACACGGCCTCTTGCGCAACGCCAAACTTTACGAAATCAACCACAACACCGAGTTGACCTTTAAAAATC
TCAAGGCAACTTTCTTTAGAACGACTCACTCTATTCCAGAGCCTTTGGGGATTGTCATTCATACTCCTCAAGGGAAAATC
GTCTGTACGGGTGACTTTAAGTTCGACTTTACTCCAGTTGGAGAACCTGCGGACTTGCATCGTATGGCTGCGCTTGGTGA
AGAAGGCGTGCTCTGTCTCCTGTCTGACTCGACAAATGCGGAAGTACCAACCTTTACCAACTCTGAAAAAGTCGTTGGTC
AGTCCATTATGAAGATTATCCAAGGTATTGAAGGACGTATCATCTTTGCATCCTTTGCCTCAAATATCTTCCGTCTCCAG
CAGGCAACAGAAGCTGCTGTTAAGACTGGACGCAAGATTGCGGTCTTTGGTCGTTCTATGGAAAAGGCCATTGTCAACGG
AATCGATCTTGGCTACATCAAAGCTCCTAAGGGAACCTTTATCGAGCCAAATGAAATCAAAGATTATCCTGCAGGAGAAG
TTCTTATCCTCTGTACAGGTAGTCAGGGTGAGCCTATGGCAGCCCTCTCTCGTATCGCCAACGGAACCCACCGTCAAGTA
CAATTACAACCAGGTGATACCGTTATCTTCTCTTCTAGTCCCATCCCTGGAAACACTACTAGCGTCAACAAGCTGATTAA
CATCATTTCTGAAGCTGGTGTCGAAGTTATCCACGGTAAAGTGAACAATATCCATACATCTGGACACGGTGGCCAGCAAG
AGCAAAAACTCATGCTCCGCTTGATTAAGCCAAAATACTTCATGCCTGTCCACGGTGAATACCGCATGCAAAAAGTCCAC
GCTGGACTAGCAGTGGATACTGGTGTTGAGAAGGACAATATCTTTATCATGAGCAATGGCGATGTGCTTGCCCTTACTGC
TGACTCAGCTCGTATCGCAGGTCATTTCAACGCCCAAGATATCTATGTCGATGGAAATCGTATCGGTGAAATTGGCGCAG
CTGTCCTCAAAGATCGTCGCGATCTATCTGAAGACGGTGTCGTTCTAGCAGTCGCAACTGTTGACTTCAAATCGCAGATG
ATTCTGTCTGGCCCAGATATCCTCAGCCGAGGCTTTGTCTACATGAGAGAGTCTGGAGACTTGATTCGCCAAAGCCAGCG
TATCCTCTTCAATGCCATTCGTATCGCACTGAAAAATAAGGATGCTAGCGTGCAATCTGTCAATGGTGCCATTGTCAACG
CTATTCGCCCCTTCCTCTATGAAAATACCGAACGTGAACCGATCATCATCCCGATGATCCTCACACCAGATGAAGAATAA

Upstream 100 bases:

>100_bases
AATCGCCCAGAGTACAATATCGAGTATATCGAACTCTTGTCTGACAAATTGCTCGATTACGAAAAAGAAACTGGCGCCTT
CGAAATTACGGAGTTCTAAT

Downstream 100 bases:

>100_bases
TACTCTTCAAACCACGTCAGCTTCGCCTTGCCGTATATATGGTTACTGACTTCGTCAGTTCTATCCACAACCTCAAAACT
GTGTTTTGAGCTGACTTCGT

Product: metallo-beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDH
IGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQV
QLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMQKVH
AGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE

Sequences:

>Translated_559_residues
MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDH
IGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQV
QLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMQKVH
AGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE
>Mature_558_residues
AYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHI
GGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKIV
CTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQQ
ATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQ
LQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMQKVHA
GLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQMI
LSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61063; Mature: 60932

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSY
CCEECCCCCCCEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCHHH
IVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKL
HHCCHHHEEEEEEECCCCCCCCCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCEE
YEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKIVCTGDFKFDFTPVGEPADLH
EEECCCCEEEEECCEEEEEEECCCCCCCCEEEEECCCCCEEEEECCEEEECCCCCCHHHH
RMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTG
HHHHHHHHCCCEEEEECCCHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEC
SQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGK
CCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECC
VNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMQKVHAGLAVDTGVEKDNIFIMSNG
CCEEEECCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHCCEEEECCCCCCCEEEEECC
DVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
CEEEEEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEE
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLY
EECCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCEECCCCHHHHHHHHHHH
ENTEREPIIIPMILTPDEE
CCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
AYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFPEDDLLGIDYVIPDYSY
CEECCCCCCCEEEECCCCCCCCCCCCCEECCCEEEEECCCCCCCCCCEEEEEECCCHHH
IVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLALALIRGKLEEHGLLRNAKL
HHCCHHHEEEEEEECCCCCCCCCCCCEEECCCCCEEECHHHHHHHHHHHHHCCCCCCCEE
YEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKIVCTGDFKFDFTPVGEPADLH
EEECCCCEEEEECCEEEEEEECCCCCCCCEEEEECCCCCEEEEECCEEEECCCCCCHHHH
RMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMKIIQGIEGRIIFASFASNIFRLQ
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHH
QATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAPKGTFIEPNEIKDYPAGEVLILCTG
HHHHHHHHCCCEEEEECCCHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEEC
SQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSPIPGNTTSVNKLINIISEAGVEVIHGK
CCCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHCCEEEEECC
VNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHGEYRMQKVHAGLAVDTGVEKDNIFIMSNG
CCEEEECCCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHCCEEEECCCCCCCEEEEECC
DVLALTADSARIAGHFNAQDIYVDGNRIGEIGAAVLKDRRDLSEDGVVLAVATVDFKSQM
CEEEEEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEECCCCEE
ILSGPDILSRGFVYMRESGDLIRQSQRILFNAIRIALKNKDASVQSVNGAIVNAIRPFLY
EECCCCHHHCCEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCEECCCCHHHHHHHHHHH
ENTEREPIIIPMILTPDEE
CCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]