Click here to switch to the map view.

The map label for this gene is ribB [H]

Identifier: 116516104

GI number: 116516104

Start: 170814

End: 172019

Strand: Reverse

Name: ribB [H]

Synonym: SPD_0167

Alternate gene names: 116516104

Gene position: 172019-170814 (Counterclockwise)

Preceding gene: 116516797

Following gene: 116517138

Centisome position: 8.41

GC content: 35.24

Gene sequence:

>1206_bases
ATGGAATATCGAAAAATACAAGAAGCATTAGAAGCATTGCAGAAGGGACGACTTGTTCTTGTTATAGACGACAAGGATAG
AGAAAATGAAGGAGACTTAATTTGTTCTGCACAAGCAGCTACAACAGAAAATGTTAATTTTATGGCTACTTATGCCAAAG
GATTAATTTGTATGCCTATGAGCGAAAGTTTAGCTAATCAATTAATGCTTTCACCTATGGTTGAAAACAATACAGATAAT
CATAAGACTGCTTTTACAGTTTCAATTGATTATAAAGAAACGACCACAGGTATTTCTGCCGAGGAAAGAGGACTGACCGC
ACGTATGTGTGTAGCTGAAGATATAACACCCTCTGATTTTCGCAGGCCAGGACACATGTTTCCTTTAATTGCAAAAAAAG
GTGGTGTCCTAGAAAGAAATGGACACACAGAAGCAACTGTTGATTTATTAAAATTAGCTGGACTAAAAGAGTGTGGCCTA
TGTTGTGAAATAATGAATCATGATGGCAAAATGATGAGAACAGATGATTTAATTCAGTTCTCGAAGAAACACAACATTCC
ATTAATTACCATCAAAGAATTACAAGAATATAGAAAAGTATATGATCAGCTGGTAGAACGAGTTTCAACTGTCAATATGC
CTACTAGATACGGTAATTTCAAAGCAATTAGCTATATAGATAAACTAAATGGGGAACATCATCTTGCTCTTATTATGGGA
AATATAGAGGATGAAGCCAATGTATTATGTCGGGTCCACTCCGAATGTTTAACAGGAGATGTTTTAGGCTCTTTACGTTG
CGATTGTGGACAGCAATTCGATAAAGCTATGAAAATGATTGTTGAGAATGGTTCGGGTGTCTTACTTTACTTGCGACAGG
AGGGACGAGGAATTGGACTTATCAATAAATTAAAAGCCTATCATTTACAAGATCAAGGCATGGATACGCTTGATGCCAAT
CTTGCATTAGGCTTTGAAGGTGATTTAAGAAAATATCATATTGGAGCACAAATGCTTAAAGATCTGGGACTTCAGTCACT
TCATTTACTGACAAATAATCCTGACAAGGTTGAACAGTTAGAAAAATATGGAATTACCATTTCCAGTAGAATATCAATCG
AAATAGAAGCCAATCCTTACGATAGTTTTTATTTAGAAACAAAGAAAAATCGAATGGGTCACATTTTAAATATGGAGGAA
AAATAA

Upstream 100 bases:

>100_bases
GGTAAATATGTGCAAAAACTGATGGATAACTCTCCAAAATCAGAAATATCTAAGGAACTATTATATCAAAATGGATTTTA
GCAGAAAGGATAATTAGTCA

Downstream 100 bases:

>100_bases
ATGAACACTTATGAAGGTAATTTAGTAGCAAACAATATTAAAATAGGTATTGTTGTAGCGAGATTTAATGAATTTATAAC
TTCAAAATTATTATCTGGAG

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTENVNFMATYAKGLICMPMSESLANQLMLSPMVENNTDN
HKTAFTVSIDYKETTTGISAEERGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYGNFKAISYIDKLNGEHHLALIMG
NIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKAMKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDAN
LALGFEGDLRKYHIGAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRMGHILNMEE
K

Sequences:

>Translated_401_residues
MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTENVNFMATYAKGLICMPMSESLANQLMLSPMVENNTDN
HKTAFTVSIDYKETTTGISAEERGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYGNFKAISYIDKLNGEHHLALIMG
NIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKAMKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDAN
LALGFEGDLRKYHIGAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRMGHILNMEE
K
>Mature_401_residues
MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTENVNFMATYAKGLICMPMSESLANQLMLSPMVENNTDN
HKTAFTVSIDYKETTTGISAEERGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYGNFKAISYIDKLNGEHHLALIMG
NIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKAMKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDAN
LALGFEGDLRKYHIGAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRMGHILNMEE
K

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=202, Percent_Identity=47.029702970297, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=50.259067357513, Blast_Score=201, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=44.4444444444444, Blast_Score=182, Evalue=7e-47,
Organism=Saccharomyces cerevisiae, GI6319438, Length=177, Percent_Identity=44.6327683615819, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 45117; Mature: 45117

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTENVNFMATYAKGLICMPM
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECC
SESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEERGLTARMCVAEDITPSDF
HHHHHHHHHHCCHHCCCCCCCEEEEEEEEECCCCCCCCCHHHCCCEEEEEEECCCCHHHH
RRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGLCCEIMNHDGKMMRTDDLIQF
CCCCCCEEEEECCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHH
SKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYGNFKAISYIDKLNGEHHLALIMG
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEEE
NIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKAMKMIVENGSGVLLYLRQEGRGIGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCH
INKLKAYHLQDQGMDTLDANLALGFEGDLRKYHIGAQMLKDLGLQSLHLLTNNPDKVEQL
HHHHHHHEECCCCCCEECCCEEECCCCCCCEEHHHHHHHHHCCCCEEEEECCCCHHHHHH
EKYGITISSRISIEIEANPYDSFYLETKKNRMGHILNMEEK
HHHCCEEEEEEEEEEECCCCCCEEEEECCCCCCCEECCCCC
>Mature Secondary Structure
MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTENVNFMATYAKGLICMPM
CCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEECC
SESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEERGLTARMCVAEDITPSDF
HHHHHHHHHHCCHHCCCCCCCEEEEEEEEECCCCCCCCCHHHCCCEEEEEEECCCCHHHH
RRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGLCCEIMNHDGKMMRTDDLIQF
CCCCCCEEEEECCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHH
SKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYGNFKAISYIDKLNGEHHLALIMG
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEEEEE
NIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKAMKMIVENGSGVLLYLRQEGRGIGL
CCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCH
INKLKAYHLQDQGMDTLDANLALGFEGDLRKYHIGAQMLKDLGLQSLHLLTNNPDKVEQL
HHHHHHHEECCCCCCEECCCEEECCCCCCCEEHHHHHHHHHCCCCEEEEECCCCHHHHHH
EKYGITISSRISIEIEANPYDSFYLETKKNRMGHILNMEEK
HHHCCEEEEEEEEEEECCCCCCEEEEECCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA