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The map label for this gene is xth

Identifier: 116516049

GI number: 116516049

Start: 1640752

End: 1641579

Strand: Direct

Name: xth

Synonym: SPD_1626

Alternate gene names: 116516049

Gene position: 1640752-1641579 (Clockwise)

Preceding gene: 116517214

Following gene: 116515812

Centisome position: 80.19

GC content: 45.29

Gene sequence:

>828_bases
ATGAAACTTATCTCATGGAATATTGATTCCCTAAACGCTGCCCTAACTAGTGACTCAGCTCGTGCCAAATTGTCCCAAGA
AGTCCTACAAACCTTGGTCGCTGAAAATGCTGATATCATTGCTATCCAAGAAACCAAGCTTTCTGCCAAAGGACCTACAA
AGAAACACGTGGAAATTTTAGAAGAACTCTTCCCAGGCTACGAAAACACGTGGCGTTCTTCCCAAGAGCCTGCCCGTAAA
GGCTATGCTGGAACCATGTTCCTTTATAAGAAAGAACTTACACCTACTATCAGCTTCCCAGAAATCGGTGCCCCTTCTAC
CATGGACTTGGAAGGTCGTATCATCACTCTAGAATTTGATGCATTTTTCGTAACCCAAGTTTACACTCCAAACGCTGGTG
ACGGTCTCAAACGCTTGGAAGAACGCCAAGTCTGGGATGCCAAATATGCTGAGTATTTGGCTGAACTAGACAAAGAAAAA
CCAGTCCTTGCGACCGGTGACTACAACGTAGCCCACAATGAAATCGACCTTGCAAATCCTGCTAGCAACCGCCGTTCACC
TGGATTTACTGACGAGGAACGTGCTGGATTTACCAACCTATTGGCAACTGGATTTACTGATACCTTCCGCCATGTTCATG
GCGATGTTCCTGAACGCTACACTTGGTGGGCACAACGCAGCAAAACTTCTAAAATCAACAATACAGGCTGGAGAATCGAC
TACTGGCTCACAAGTAACCGTATCGCTGACAAGGTCACTAAATCTGATATGATTGACTCAGGTGCTCGCCAAGACCATAC
ACCGATTGTCTTGGAAATTGATTTGTAA

Upstream 100 bases:

>100_bases
ATTATAAACGAAAGAGAGTAATTTTTCAAACCATACGTTGGGGAAATAGATCTTTTTTTGGTATAATAAAATCTATAATC
TGAATGAAAAAGGTAACTTT

Downstream 100 bases:

>100_bases
GGATTTTCTATATGGACTACAATGCGGTCATTCCCGAGTTGCTTGTATCGAATATCGAACAGTCCCGGTCCTTCTACTGT
GGTTTACTGGGTTTTAGGAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARK
GYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL

Sequences:

>Translated_275_residues
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARK
GYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL
>Mature_275_residues
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARK
GYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL

Specific function: In addition to 3'- to 5'-exonuclease and 3'-phosphatase activities, ExoA was shown to make single-strand breaks at apurinic sites in DNA

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family

Homologues:

Organism=Homo sapiens, GI18375505, Length=276, Percent_Identity=37.3188405797101, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI18375503, Length=276, Percent_Identity=37.3188405797101, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI18375501, Length=276, Percent_Identity=37.3188405797101, Blast_Score=172, Evalue=2e-43,
Organism=Escherichia coli, GI1788046, Length=298, Percent_Identity=27.8523489932886, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=39.2727272727273, Blast_Score=189, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI71989539, Length=162, Percent_Identity=32.0987654320988, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI221330655, Length=273, Percent_Identity=36.6300366300366, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17136678, Length=273, Percent_Identity=36.6300366300366, Blast_Score=167, Evalue=8e-42,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): EXOA_STRPN (P0A2X3)

Other databases:

- EMBL:   AE005672
- PIR:   E95215
- RefSeq:   NP_346278.1
- ProteinModelPortal:   P0A2X3
- SMR:   P0A2X3
- EnsemblBacteria:   EBSTRT00000026693
- GeneID:   930987
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_1845
- TIGR:   SP_1845
- GeneTree:   EBGT00050000027380
- HOGENOM:   HBG704164
- OMA:   TGPTKKH
- ProtClustDB:   CLSK2752120
- BioCyc:   SPNE170187-1:SP_1845-MONOMER
- BRENDA:   3.1.11.2
- GO:   GO:0005737
- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808
- PANTHER:   PTHR22748
- TIGRFAMs:   TIGR00195
- TIGRFAMs:   TIGR00633

Pfam domain/function: PF03372 Exo_endo_phos; SSF56219 Exo_endo_phos

EC number: =3.1.11.2

Molecular weight: Translated: 31064; Mature: 31064

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00726 AP_NUCLEASE_F1_1; PS00727 AP_NUCLEASE_F1_2; PS00728 AP_NUCLEASE_F1_3; PS51435 AP_NUCLEASE_F1_4

Important sites: ACT_SITE 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEIL
CEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
EELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFD
HHHCCCCCHHCCCCCCHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCEEEEEEEC
AFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANP
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCC
ASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
CCCCCCCCCCCHHHCCHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEE
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL
EEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEIL
CEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHH
EELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFD
HHHCCCCCHHCCCCCCHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCEEEEEEEC
AFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANP
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECCCC
ASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRID
CCCCCCCCCCCHHHCCHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEE
YWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL
EEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916