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The map label for this gene is murB

Identifier: 116515736

GI number: 116515736

Start: 1254158

End: 1255108

Strand: Reverse

Name: murB

Synonym: SPD_1222

Alternate gene names: 116515736

Gene position: 1255108-1254158 (Counterclockwise)

Preceding gene: 116516196

Following gene: 116516399

Centisome position: 61.34

GC content: 45.11

Gene sequence:

>951_bases
ATGTCTGTAAGAGAAAAAATGCTTGAAATCTTAGAAGGAATTGATATCCGTTTTAAGGAACCCTTGCATAGCTATAGTTA
TACAAAAGTAGGTGGAGAGGCTGATTATTTGGTCTTTCCACGAAATCGTTTTGAGTTGGCTCGCGTTGTGAAATTTGCCA
ACCAAGAAAATATCCCTTGGATGGTTCTTGGCAATGCAAGCAATATCATCGTTCGTGATGGTGGGATTCGTGGATTTGTC
ATCTTGTGTGACAAGCTCAATAACGTTTCTGTTGATGGCTATACCATTGAAGCAGAAGCTGGGGCTAACTTGATTGAAAC
AACTCGCATTGCCCTCCGTCATAGTTTAACTGGCTTTGAGTTTGCTTGTGGTATTCCAGGAAGCGTTGGCGGTGCTGTCT
TTATGAATGCGGGTGCCTATGGTGGCGAGATTGCTCACATCTTGCAGTCTTGTAAGGTCTTGACCAAGGATGGAGAAATC
GAAACCCTGTCTGCTAAAGACTTGGCTTTTGGTTACCGCCATTCAGCTATTCAGGAGTCTGGTGCAGTTGTCTTGTCAGT
TAAATTTGCCCTAGCTCCAGGAACCCATCAGGTTATCAAGCAGGAAATGGACCGCTTGACGCACCTACGTGAACTCAAGC
AACCTTTGGAATACCCATCTTGTGGCTCGGTCTTTAAGCGTCCAGTCGGGCATTTTGCAGGTCAGTTAATTTCAGAAGCT
GGCTTGAAAGGCTATCGTATCGGTGGCGTAGAAGTGTCAGAAAAGCATGCAGGATTTATGATCAATGTCGCAGATGGAAC
GGCCAAAGACTACGAGGACTTGATCCAATCGGTTATCGAAAAAGTCAAGGAACACTCAGGTATTACGCTTGAAAGAGAAG
TCCGGATCTTGGGTGAAAGCCTATCGGTAGCGAAGATGTATGCAGGTGGTTTTACTCCCTGCAAGAGGTAG

Upstream 100 bases:

>100_bases
CCTCTTTTTCTTATGCAAAATCCAAACAAGGTATTTTTATGGTCGTCAAATGTCTCTAAAAATGGTATAATGGAATGAAT
TTTGTAAAAGGAAGAGTGAC

Downstream 100 bases:

>100_bases
TGGGGACCTGACAGAGCCCCGATCGGTTAATCTATGAAAAAGAAGGAATTTATGCCAATTGAAAAAACCAATTATCGAAT
TCAAAAACGTCTCTAAAGTT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRFELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFV
ILCDKLNNVSVDGYTIEAEAGANLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEYPSCGSVFKRPVGHFAGQLISEA
GLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQSVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR

Sequences:

>Translated_316_residues
MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRFELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFV
ILCDKLNNVSVDGYTIEAEAGANLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEYPSCGSVFKRPVGHFAGQLISEA
GLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQSVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR
>Mature_315_residues
SVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRFELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVI
LCDKLNNVSVDGYTIEAEAGANLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEIE
TLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEYPSCGSVFKRPVGHFAGQLISEAG
LKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQSVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=338, Percent_Identity=25.7396449704142, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_STRP2 (Q04JV9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816689.1
- ProteinModelPortal:   Q04JV9
- SMR:   Q04JV9
- STRING:   Q04JV9
- EnsemblBacteria:   EBSTRT00000020350
- GeneID:   4441024
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1222
- eggNOG:   COG0812
- GeneTree:   EBGT00050000027116
- HOGENOM:   HBG686573
- OMA:   SKKHAGF
- ProtClustDB:   PRK13905
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 34539; Mature: 34408

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 173-173 ACT_SITE 223-223 ACT_SITE 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRFELARVVKFANQENIPW
CCHHHHHHHHHCCCCCEECCCHHCCCCEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCE
MVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGANLIETTRIALRHSLTGFE
EEECCCCEEEEECCCCEEEEEEEECCCCEEECCEEEECCCCCCHHHHHHHHHHHCCCCCE
FACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEIETLSAKDLAFGYRHSAIQES
EECCCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHHHCC
GAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEYPSCGSVFKRPVGHFAGQLISEA
CCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQSVIEKVKEHSGITLEREVRILGES
CCCCEEECCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCC
LSVAKMYAGGFTPCKR
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRFELARVVKFANQENIPW
CHHHHHHHHHCCCCCEECCCHHCCCCEECCCCCCEEEECCCHHHHHHHHHHCCCCCCCE
MVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGANLIETTRIALRHSLTGFE
EEECCCCEEEEECCCCEEEEEEEECCCCEEECCEEEECCCCCCHHHHHHHHHHHCCCCCE
FACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEIETLSAKDLAFGYRHSAIQES
EECCCCCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHHHCC
GAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEYPSCGSVFKRPVGHFAGQLISEA
CCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
GLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQSVIEKVKEHSGITLEREVRILGES
CCCCEEECCEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHCCC
LSVAKMYAGGFTPCKR
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA