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The map label for this gene is birA [H]

Identifier: 116515717

GI number: 116515717

Start: 1696527

End: 1697462

Strand: Direct

Name: birA [H]

Synonym: SPD_1680

Alternate gene names: 116515717

Gene position: 1696527-1697462 (Clockwise)

Preceding gene: 116516533

Following gene: 116516281

Centisome position: 82.91

GC content: 36.11

Gene sequence:

>936_bases
ATGAAATCCTACCAAGCTGTCTACCAAATCCTATCTAAAGAAACCGACTATATCAGCGGAGAAAAAATCGCAGAAAAACT
ATCCCTAAGCCGAACATCAATTTGGAAAGCTATCAAGCGACTAGAACAAGAAGGCATTGAAATTAATAGTATCAAAAATA
GAGGATATAAACTAATGAATGGTGACCTTATTCTTCCAGAGATTCTAGAAGAAAATCTTCCAATTAAAGTCAGCTTTAAA
CCCGAAACAAAATCAACACAACTAGATGCAAAAGAAGCAATTGATTTAGGCCATGAAGCAAATACTCTCTATCTAGCTTC
CTATCAAACAGCAGGCCGAGGCCGTTTTCAACGTTCCTTCTACTCACCACAAGGTGGAATTTATATGACACTCCATCTTA
AACCAAATCTCCCCTATGACAAATTACCATCCTACACACTACTTGTAGCTGGAGCTGTCTACAAAGCCATTAAGAACCTA
ACTTTAATAGATGTCGATATAAAATGGGTCAATGATATCTATCTAAACAATCATAAAATTGGAGGAATCCTTACTGAAGC
AATGACCTCTGTAGAAACTGGCTTAGTCACAGATATCATTATTGGAGTAGGTATCAATTTCACTATTAAAGACTTCCCTC
AGGAATTAAAAGAAAAAGCTGCCAGCTTATTTAAAGCTACAGCTCCTATAACAAGGAATGAATTGATCATAGAAATCTGG
CGTGCTTTCTTCGAAACACCAGCAGAAGAGCTATTATACCTATACAAAAAACAGTCATTCATTCTAGGAAAAGAAGTCAC
TTTCACACTAGAGCAAAAAGACTACAAGGGACTTGCTAAAGACATCTCAGAAAATGGAAAACTTTTAGTTCAATGTGATA
ACGGAAAAGAAATCTGGCTAAATAGTGGCGAAATTTCTCTCAATAGTTGGAAGTAA

Upstream 100 bases:

>100_bases
CACTCCATTTTTCGAAAGCTTATAAACAATACTTTAATCAGTCTCCAAGTCATACAAGAAAAGAATACTCTCAATACCAA
CTAGTGAGAAAGGCAACATT

Downstream 100 bases:

>100_bases
AATAACACAATTATAATATAAACGATATAAAAATAACTTCAGATTAGTAATTCAATTAAGTTTTACGGATCTGAAGTTTT
ATTGTCTCTATAATATTTGT

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFK
PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAASLFKATAPITRNELIIEIW
RAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK

Sequences:

>Translated_311_residues
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFK
PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAASLFKATAPITRNELIIEIW
RAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK
>Mature_311_residues
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFK
PETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAASLFKATAPITRNELIIEIW
RAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=312, Percent_Identity=28.525641025641, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35243; Mature: 35243

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMN
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEC
GDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSF
CCEECHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHH
YSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKI
CCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCC
GGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAASLFKATAPITRNELIIEIW
CHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
RAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWL
HHHHHCCHHHHHHHHHHCCEEECCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCEEEE
NSGEISLNSWK
ECCEEEECCCC
>Mature Secondary Structure
MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMN
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEC
GDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSF
CCEECHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHH
YSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKI
CCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCCCC
GGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAASLFKATAPITRNELIIEIW
CHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
RAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWL
HHHHHCCHHHHHHHHHHCCEEECCEEEEEECCCHHHHHHHHHCCCCCEEEEECCCCEEEE
NSGEISLNSWK
ECCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]