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The map label for this gene is gltX

Identifier: 116514985

GI number: 116514985

Start: 46858

End: 48282

Strand: Direct

Name: gltX

Synonym: BCc_042

Alternate gene names: 116514985

Gene position: 46858-48282 (Clockwise)

Preceding gene: 116514984

Following gene: 116514986

Centisome position: 11.25

GC content: 18.81

Gene sequence:

>1425_bases
ATGAAAATTAAAACAAGATTTTCTCCAAGTCCAACTGGTTTACTTCATATGGGAGGAGTTCGTACTGCATTGTATTCATG
GCTTTTTGCCCGTAAAAATAATGGTTCTTTTATTTTACGAATTGAAGATACTGATAAAAATAGAGTTAAAAATAATTCAG
TACAAGATATTTTATATGGATTAAGATATTTACAATTAAATTGGGATGAAGGGCCATTTTTTCAAAGTAATAGAATTAAT
ATATATAAAAATATAATATTATTTATGTTAAAAAAAGGTATTGCATATAAATGTTATTGTTCTAAAAATCGTCTAGATAA
ATTACGAAAAAATCAAATTTTAAATAAAAAAAAACCTAAATATGATAATAAATGTCGAAATAAAAATTTTTTTTTAAAAA
AATCTAATATTCCTTATGTAATTCGTTTTAAGAATCCTACAAAAGGATTAGTAGAATTTAGAGATATGATTAGAGGAAAA
ATTAGTATTTCAAATAAAGAATTAGATGATTTAGTAATTCAAAGATCTAATGGAATGCCAACATATAATTTTTGTGTTGT
AGTAGATGATTGGCAAATGAATATTACACATATTATCAGAGGTGAAGATCATATTCATAATACACCAAGACAAATTAATT
TATTATCTTCTTTAAACGCATATATTCCTCAATATGCACATACATCAATGATTTTAGATAAAAAAAGAAAAAAACTATCT
AAACGTTGTTCTTCATATAGTATTATTAATTATATTAATAATGGTTTTATACCTGAGGCTATTTTAAATTATGCTCTTCA
ATTAGGTTGGTCTTATAAAAATCAAGAAATATTTTCAATTAACGAAATGAAAAATATATTTAATATTAAGTATATTAATA
AATCACCAAGCATTATAGATAAAAAAAAATTTTTATGGTTTAATCATTATTATTTAAACAATATATCTTTTAATTTAAAA
TATAAATATTTTTTTTCTTATTGTAAAAAAAATAATATTTTTTTTGACAAGGATGTAAATATATCTGGAGTTATAAAAGA
TTTCTTAGGTCGTCATAGTACTTTTAAAGATTTTATTCAAACATATGATTATTTTTATAAAGAAATTAATATTTCTAATA
TAAAAAATATATATTTATATAATAAATTAATTAATATTACTATATTGAAATTTTTATATAAGAAATTCAATTTGTTAAAT
AATTGGGATTTAAAAAATATTTTATTAATAATTAAAGAATCAATTTTATATTTTAAAATTTCTTTTAAGGAAATAGCTAT
TTTAATTAGAATTGTTATTACAGGAAAAAAACAAACACCTAGTATTTCTTCTATAATTTTTTATATAGGAAAAAAAAAAT
TTTTATTAAGAATTAAAAATTTTTTGAAATATCTGCAATTAAATAATTCAAATTTTAGTAAATAA

Upstream 100 bases:

>100_bases
TACTTATATTTGTTTATTTGGTTAAAAAATATACAAATATTAATGAAAATTATATATTTAATTTTATTTTTAATAATTTC
ATTATTTAAATTGAGTATTT

Downstream 100 bases:

>100_bases
TTTTAAAATTATTCAAAACTTTATATTTTGATTCAATATATTTTTAATTATTATTAACATATTATATGTTAAAATATAAA
AAATTTTTATATTAAATATT

Product: GltX

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKIKTRFSPSPTGLLHMGGVRTALYSWLFARKNNGSFILRIEDTDKNRVKNNSVQDILYGLRYLQLNWDEGPFFQSNRIN
IYKNIILFMLKKGIAYKCYCSKNRLDKLRKNQILNKKKPKYDNKCRNKNFFLKKSNIPYVIRFKNPTKGLVEFRDMIRGK
ISISNKELDDLVIQRSNGMPTYNFCVVVDDWQMNITHIIRGEDHIHNTPRQINLLSSLNAYIPQYAHTSMILDKKRKKLS
KRCSSYSIINYINNGFIPEAILNYALQLGWSYKNQEIFSINEMKNIFNIKYINKSPSIIDKKKFLWFNHYYLNNISFNLK
YKYFFSYCKKNNIFFDKDVNISGVIKDFLGRHSTFKDFIQTYDYFYKEINISNIKNIYLYNKLINITILKFLYKKFNLLN
NWDLKNILLIIKESILYFKISFKEIAILIRIVITGKKQTPSISSIIFYIGKKKFLLRIKNFLKYLQLNNSNFSK

Sequences:

>Translated_474_residues
MKIKTRFSPSPTGLLHMGGVRTALYSWLFARKNNGSFILRIEDTDKNRVKNNSVQDILYGLRYLQLNWDEGPFFQSNRIN
IYKNIILFMLKKGIAYKCYCSKNRLDKLRKNQILNKKKPKYDNKCRNKNFFLKKSNIPYVIRFKNPTKGLVEFRDMIRGK
ISISNKELDDLVIQRSNGMPTYNFCVVVDDWQMNITHIIRGEDHIHNTPRQINLLSSLNAYIPQYAHTSMILDKKRKKLS
KRCSSYSIINYINNGFIPEAILNYALQLGWSYKNQEIFSINEMKNIFNIKYINKSPSIIDKKKFLWFNHYYLNNISFNLK
YKYFFSYCKKNNIFFDKDVNISGVIKDFLGRHSTFKDFIQTYDYFYKEINISNIKNIYLYNKLINITILKFLYKKFNLLN
NWDLKNILLIIKESILYFKISFKEIAILIRIVITGKKQTPSISSIIFYIGKKKFLLRIKNFLKYLQLNNSNFSK
>Mature_474_residues
MKIKTRFSPSPTGLLHMGGVRTALYSWLFARKNNGSFILRIEDTDKNRVKNNSVQDILYGLRYLQLNWDEGPFFQSNRIN
IYKNIILFMLKKGIAYKCYCSKNRLDKLRKNQILNKKKPKYDNKCRNKNFFLKKSNIPYVIRFKNPTKGLVEFRDMIRGK
ISISNKELDDLVIQRSNGMPTYNFCVVVDDWQMNITHIIRGEDHIHNTPRQINLLSSLNAYIPQYAHTSMILDKKRKKLS
KRCSSYSIINYINNGFIPEAILNYALQLGWSYKNQEIFSINEMKNIFNIKYINKSPSIIDKKKFLWFNHYYLNNISFNLK
YKYFFSYCKKNNIFFDKDVNISGVIKDFLGRHSTFKDFIQTYDYFYKEINISNIKNIYLYNKLINITILKFLYKKFNLLN
NWDLKNILLIIKESILYFKISFKEIAILIRIVITGKKQTPSISSIIFYIGKKKFLLRIKNFLKYLQLNNSNFSK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=28.5132382892057, Blast_Score=230, Evalue=3e-60,
Organism=Escherichia coli, GI1788743, Length=465, Percent_Identity=42.5806451612903, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI226510925, Length=238, Percent_Identity=34.0336134453782, Blast_Score=144, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=29.8755186721992, Blast_Score=209, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=494, Percent_Identity=31.3765182186235, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=214, Percent_Identity=28.5046728971963, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=28.8888888888889, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_BUCCC (Q058C8)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802614.1
- ProteinModelPortal:   Q058C8
- SMR:   Q058C8
- STRING:   Q058C8
- EnsemblBacteria:   EBBUCT00000001429
- GeneID:   4440970
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_042
- eggNOG:   COG0008
- GeneTree:   EBGT00050000008076
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- BioCyc:   BAPH372461:BCC_042-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56541; Mature: 56541

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 241-241

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKTRFSPSPTGLLHMGGVRTALYSWLFARKNNGSFILRIEDTDKNRVKNNSVQDILYG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCCHHHHHHC
LRYLQLNWDEGPFFQSNRINIYKNIILFMLKKGIAYKCYCSKNRLDKLRKNQILNKKKPK
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCC
YDNKCRNKNFFLKKSNIPYVIRFKNPTKGLVEFRDMIRGKISISNKELDDLVIQRSNGMP
CCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCHHHHHEECCCCCC
TYNFCVVVDDWQMNITHIIRGEDHIHNTPRQINLLSSLNAYIPQYAHTSMILDKKRKKLS
EEEEEEEEECCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KRCSSYSIINYINNGFIPEAILNYALQLGWSYKNQEIFSINEMKNIFNIKYINKSPSIID
HHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHCEEEEEEECCCCCCCC
KKKFLWFNHYYLNNISFNLKYKYFFSYCKKNNIFFDKDVNISGVIKDFLGRHSTFKDFIQ
CCCEEEEEEEEEECEEEEEEEHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCHHHHHHH
TYDYFYKEINISNIKNIYLYNKLINITILKFLYKKFNLLNNWDLKNILLIIKESILYFKI
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEE
SFKEIAILIRIVITGKKQTPSISSIIFYIGKKKFLLRIKNFLKYLQLNNSNFSK
EHHHHHEEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MKIKTRFSPSPTGLLHMGGVRTALYSWLFARKNNGSFILRIEDTDKNRVKNNSVQDILYG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECCCCCCCCCCCHHHHHHC
LRYLQLNWDEGPFFQSNRINIYKNIILFMLKKGIAYKCYCSKNRLDKLRKNQILNKKKPK
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCCC
YDNKCRNKNFFLKKSNIPYVIRFKNPTKGLVEFRDMIRGKISISNKELDDLVIQRSNGMP
CCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHCCEEEECCCCHHHHHEECCCCCC
TYNFCVVVDDWQMNITHIIRGEDHIHNTPRQINLLSSLNAYIPQYAHTSMILDKKRKKLS
EEEEEEEEECCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KRCSSYSIINYINNGFIPEAILNYALQLGWSYKNQEIFSINEMKNIFNIKYINKSPSIID
HHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHCEEEEEEECCCCCCCC
KKKFLWFNHYYLNNISFNLKYKYFFSYCKKNNIFFDKDVNISGVIKDFLGRHSTFKDFIQ
CCCEEEEEEEEEECEEEEEEEHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCHHHHHHH
TYDYFYKEINISNIKNIYLYNKLINITILKFLYKKFNLLNNWDLKNILLIIKESILYFKI
HHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEE
SFKEIAILIRIVITGKKQTPSISSIIFYIGKKKFLLRIKNFLKYLQLNNSNFSK
EHHHHHEEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA