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The map label for this gene is metF [H]

Identifier: 116514975

GI number: 116514975

Start: 32539

End: 33420

Strand: Direct

Name: metF [H]

Synonym: BCc_030

Alternate gene names: 116514975

Gene position: 32539-33420 (Clockwise)

Preceding gene: 116514966

Following gene: 116514976

Centisome position: 7.81

GC content: 19.84

Gene sequence:

>882_bases
ATGGTTACAGAAATAAAAAATATTAAAAAATTATATACTGATAAAATATATAATTTAAAAAATAAAATTAAAATATCTTT
CGAAGTATTTCCTCCTGGAGATATATTTTTACAGAAGAAATTATTTAAATCTATTAAAATATTAAATCAATTTAATCCAG
AATTTTTTTCAGTTACTAATAGTACAAAAATTAATAGTTTTGATCAGACATTTTTTATTGTTAAAAAAATACGTGAATTA
ATTCATTGTACTGTTTTTGCTCATTTTACTACTATAGGATGTAATGAATTAAAAATAGAAAAGATAGCACATCGATATTG
GAATCATGGAATTAAAAATATTATTGCTTTAAGAGGTGATTTACCTGTATTATATTCTAGAAAAATAATATATGCAGTAG
ATCTAATTAAATTATTAAATTCAATAAATAAATTTAAAATATTTGTTGCTGCTTATCCTGAAGGACATCCAGAGTCAAAA
AATATTCAAGAAGATTTAAATAATTTAAGAAATAAATTAGATTTAGGAGTGAAACAAGCTATTACTCAATTTTTTTTTAA
TATTGATAAATTTTTAAAATTTCGAGATACATGTTTTTCTTTAGGATTAAATGTAAATTTAATACCAGGTATTTTGCCTA
TTTTAAATTTTGAACAATTAAAAAAATTTTCTAATATGACAAATGTTGAAGTTCCAAAATGGATTTTAAATACTTTTCAT
AGACATAAAAATGATGAAAAAAAATGTATTTCTTGTAGTGTATATATTATTATTGATTTAATTATAAAATTATATAATGA
AGGAATTACACATTTTCATTTATATACATTAAATCAGCCCCATCTAATTTCTTTAATTTGCCAACGATTAGGTTTAGTAT
AA

Upstream 100 bases:

>100_bases
TTAAAAAAATATAGATTTTTCATTTAAATGAAATATATTTTTTTATATTTTTTTCTTATATAATATTATTTATATTAATT
AATATAATTTGGATATATAT

Downstream 100 bases:

>100_bases
TTTTTAAAAATATATAATATATATTTTTAAAAATTAATAAAAATTAAAATAATATTTTAAAAAATTTCTTATTTTAATTG
TTTTTTAATTTAATAATATT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MVTEIKNIKKLYTDKIYNLKNKIKISFEVFPPGDIFLQKKLFKSIKILNQFNPEFFSVTNSTKINSFDQTFFIVKKIREL
IHCTVFAHFTTIGCNELKIEKIAHRYWNHGIKNIIALRGDLPVLYSRKIIYAVDLIKLLNSINKFKIFVAAYPEGHPESK
NIQEDLNNLRNKLDLGVKQAITQFFFNIDKFLKFRDTCFSLGLNVNLIPGILPILNFEQLKKFSNMTNVEVPKWILNTFH
RHKNDEKKCISCSVYIIIDLIIKLYNEGITHFHLYTLNQPHLISLICQRLGLV

Sequences:

>Translated_293_residues
MVTEIKNIKKLYTDKIYNLKNKIKISFEVFPPGDIFLQKKLFKSIKILNQFNPEFFSVTNSTKINSFDQTFFIVKKIREL
IHCTVFAHFTTIGCNELKIEKIAHRYWNHGIKNIIALRGDLPVLYSRKIIYAVDLIKLLNSINKFKIFVAAYPEGHPESK
NIQEDLNNLRNKLDLGVKQAITQFFFNIDKFLKFRDTCFSLGLNVNLIPGILPILNFEQLKKFSNMTNVEVPKWILNTFH
RHKNDEKKCISCSVYIIIDLIIKLYNEGITHFHLYTLNQPHLISLICQRLGLV
>Mature_293_residues
MVTEIKNIKKLYTDKIYNLKNKIKISFEVFPPGDIFLQKKLFKSIKILNQFNPEFFSVTNSTKINSFDQTFFIVKKIREL
IHCTVFAHFTTIGCNELKIEKIAHRYWNHGIKNIIALRGDLPVLYSRKIIYAVDLIKLLNSINKFKIFVAAYPEGHPESK
NIQEDLNNLRNKLDLGVKQAITQFFFNIDKFLKFRDTCFSLGLNVNLIPGILPILNFEQLKKFSNMTNVEVPKWILNTFH
RHKNDEKKCISCSVYIIIDLIIKLYNEGITHFHLYTLNQPHLISLICQRLGLV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=29.0780141843972, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1790377, Length=278, Percent_Identity=41.3669064748201, Blast_Score=247, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=28.0575539568345, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=28.0575539568345, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325234, Length=296, Percent_Identity=29.3918918918919, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321313, Length=287, Percent_Identity=25.7839721254355, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34310; Mature: 34310

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTEIKNIKKLYTDKIYNLKNKIKISFEVFPPGDIFLQKKLFKSIKILNQFNPEFFSVTN
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECC
STKINSFDQTFFIVKKIRELIHCTVFAHFTTIGCNELKIEKIAHRYWNHGIKNIIALRGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
LPVLYSRKIIYAVDLIKLLNSINKFKIFVAAYPEGHPESKNIQEDLNNLRNKLDLGVKQA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ITQFFFNIDKFLKFRDTCFSLGLNVNLIPGILPILNFEQLKKFSNMTNVEVPKWILNTFH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
RHKNDEKKCISCSVYIIIDLIIKLYNEGITHFHLYTLNQPHLISLICQRLGLV
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVTEIKNIKKLYTDKIYNLKNKIKISFEVFPPGDIFLQKKLFKSIKILNQFNPEFFSVTN
CCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECC
STKINSFDQTFFIVKKIRELIHCTVFAHFTTIGCNELKIEKIAHRYWNHGIKNIIALRGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
LPVLYSRKIIYAVDLIKLLNSINKFKIFVAAYPEGHPESKNIQEDLNNLRNKLDLGVKQA
CCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ITQFFFNIDKFLKFRDTCFSLGLNVNLIPGILPILNFEQLKKFSNMTNVEVPKWILNTFH
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
RHKNDEKKCISCSVYIIIDLIIKLYNEGITHFHLYTLNQPHLISLICQRLGLV
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA