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The map label for this gene is yqiG [H]

Identifier: 116495855

GI number: 116495855

Start: 2396682

End: 2397845

Strand: Direct

Name: yqiG [H]

Synonym: LSEI_2409

Alternate gene names: 116495855

Gene position: 2396682-2397845 (Clockwise)

Preceding gene: 116495854

Following gene: 116495860

Centisome position: 82.78

GC content: 51.55

Gene sequence:

>1164_bases
ATGTCGGGTTATCATTTTTTAAAACCGTTCACCTTCAAGCACCAAACCATCACGTTGAAAAATCGCATTGTCATTCCGCC
GATGACAACGCGCCTGTCGTTTGAAGACGGCACTGTGACGCGGGACGAAATCAGGTATTACCAGCAACGGGCCGGTGGCG
TCGGCATGTTTATTACCGGTACCGCCAATGTCAATGCGCTTGGCAAAGGATTCGAAGGTGAGCTAAGTGTCGCTGATGAT
CGATTCATCCCCGGTTTGAGCAAACTCGCCGCTGCGATGAAAACAGGCGGCACCAAGGCTATTTTGCAAATCTTCAGCGC
TGGCCGCATGAGCAATAGCAAGATTCTGCGCGGTGAGCAGCCAGTCAGTGCCAGTGCGGTTGCTGCGCCACGGGCGGGTT
ACGAAACACCGCGCGCATTGACATCGGCAGAAATCGAGGCCACTATTCACGATTTTGGTCAGGCTGTGCGCCGCGCCATC
CTTGCTGGTTTTGATGGCATCGAACTGCATGGTGCCAACACCTATCTGATACAGCAATTCTATTCGCCAAACTCTAATCG
CCGAACCGATGAATGGGGCGGTGACCGCGATAAGCGGATGCGTTTTCCGTTGGCAGTCGTTCATGAAGCAGAAAAAGTGA
TTGCCACAATTGCTGACCGGCCATTTTTGTTAGGCTACCGCATTTCGCCGGAAGAGTTGGAACAGCCTGGCATTACCTTG
GATGATACCTTGGCCTTAATCGACACTTTGAAACAAACCAAGATTGATTACTTGCACGTCTCCCAAAGTGACGTTTGGCG
AACCTCATTGCGCAACCCCGAGGACACAGCCATCATGAATGAACAAATTCGTGACCATGTCGCCGGTGCTTTTCCAGTGA
TTGTGGTCGGTGGCATTAAAACCCCCGCCGATGCTGAGAAAGCGGCAGAATCCTTTGATTTGGTCGCGATTGGTCACGAA
ATGATTCGCGAGCCGCACTGGGTTCAGAAGGTCTTAGACCATGACGAAAAGGCGATCCGTTACCAGATCGCCCCTGCTGA
TCTTGAAGAACTTGGCATTGCGCCGACCTTCCTCGATTTTATCGAAAGTATTTCCGGTGGGGCAAAAGGTGTCCCGTTAA
CAACTGCGCAGTCTGTGACATCAAGCAACGTGACTCAAGATTAA

Upstream 100 bases:

>100_bases
TTGTATGCCATTGATCAACCGGCCAATGTTGCCATCAATGAAGTGCTCTTACGGCCAGTCACGCAAGAACGTTAATTTTC
GTCTAAAGGAGTGTGATTGC

Downstream 100 bases:

>100_bases
TCTAGCATTTCAAATCATCGTGCGAGTCGAGCTGTAAAAAGCTCGGCTTTTTTCTTGCCGTCTTCAACTTACGCGATGGT
GCCTGAAGCTACCAGTGATT

Product: NADH:flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSGYHFLKPFTFKHQTITLKNRIVIPPMTTRLSFEDGTVTRDEIRYYQQRAGGVGMFITGTANVNALGKGFEGELSVADD
RFIPGLSKLAAAMKTGGTKAILQIFSAGRMSNSKILRGEQPVSASAVAAPRAGYETPRALTSAEIEATIHDFGQAVRRAI
LAGFDGIELHGANTYLIQQFYSPNSNRRTDEWGGDRDKRMRFPLAVVHEAEKVIATIADRPFLLGYRISPEELEQPGITL
DDTLALIDTLKQTKIDYLHVSQSDVWRTSLRNPEDTAIMNEQIRDHVAGAFPVIVVGGIKTPADAEKAAESFDLVAIGHE
MIREPHWVQKVLDHDEKAIRYQIAPADLEELGIAPTFLDFIESISGGAKGVPLTTAQSVTSSNVTQD

Sequences:

>Translated_387_residues
MSGYHFLKPFTFKHQTITLKNRIVIPPMTTRLSFEDGTVTRDEIRYYQQRAGGVGMFITGTANVNALGKGFEGELSVADD
RFIPGLSKLAAAMKTGGTKAILQIFSAGRMSNSKILRGEQPVSASAVAAPRAGYETPRALTSAEIEATIHDFGQAVRRAI
LAGFDGIELHGANTYLIQQFYSPNSNRRTDEWGGDRDKRMRFPLAVVHEAEKVIATIADRPFLLGYRISPEELEQPGITL
DDTLALIDTLKQTKIDYLHVSQSDVWRTSLRNPEDTAIMNEQIRDHVAGAFPVIVVGGIKTPADAEKAAESFDLVAIGHE
MIREPHWVQKVLDHDEKAIRYQIAPADLEELGIAPTFLDFIESISGGAKGVPLTTAQSVTSSNVTQD
>Mature_386_residues
SGYHFLKPFTFKHQTITLKNRIVIPPMTTRLSFEDGTVTRDEIRYYQQRAGGVGMFITGTANVNALGKGFEGELSVADDR
FIPGLSKLAAAMKTGGTKAILQIFSAGRMSNSKILRGEQPVSASAVAAPRAGYETPRALTSAEIEATIHDFGQAVRRAIL
AGFDGIELHGANTYLIQQFYSPNSNRRTDEWGGDRDKRMRFPLAVVHEAEKVIATIADRPFLLGYRISPEELEQPGITLD
DTLALIDTLKQTKIDYLHVSQSDVWRTSLRNPEDTAIMNEQIRDHVAGAFPVIVVGGIKTPADAEKAAESFDLVAIGHEM
IREPHWVQKVLDHDEKAIRYQIAPADLEELGIAPTFLDFIESISGGAKGVPLTTAQSVTSSNVTQD

Specific function: Unknown

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=351, Percent_Identity=32.1937321937322, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789463, Length=235, Percent_Identity=32.3404255319149, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540738, Length=363, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=339, Percent_Identity=26.5486725663717, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=259, Percent_Identity=30.8880308880309, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17559804, Length=275, Percent_Identity=29.8181818181818, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=262, Percent_Identity=31.6793893129771, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=264, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=269, Percent_Identity=25.2788104089219, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=225, Percent_Identity=30.6666666666667, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=231, Percent_Identity=30.3030303030303, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 42452; Mature: 42320

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGYHFLKPFTFKHQTITLKNRIVIPPMTTRLSFEDGTVTRDEIRYYQQRAGGVGMFITG
CCCCCCCCCEEECCEEEEECCEEEECCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEEE
TANVNALGKGFEGELSVADDRFIPGLSKLAAAMKTGGTKAILQIFSAGRMSNSKILRGEQ
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCC
PVSASAVAAPRAGYETPRALTSAEIEATIHDFGQAVRRAILAGFDGIELHGANTYLIQQF
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHH
YSPNSNRRTDEWGGDRDKRMRFPLAVVHEAEKVIATIADRPFLLGYRISPEELEQPGITL
HCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCH
DDTLALIDTLKQTKIDYLHVSQSDVWRTSLRNPEDTAIMNEQIRDHVAGAFPVIVVGGIK
HHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
TPADAEKAAESFDLVAIGHEMIREPHWVQKVLDHDEKAIRYQIAPADLEELGIAPTFLDF
CCCHHHHHHHHCCEEEECHHHHHCCHHHHHHHCCCCCEEEEEECCCCHHHHCCCHHHHHH
IESISGGAKGVPLTTAQSVTSSNVTQD
HHHHCCCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SGYHFLKPFTFKHQTITLKNRIVIPPMTTRLSFEDGTVTRDEIRYYQQRAGGVGMFITG
CCCCCCCCEEECCEEEEECCEEEECCCCEEEECCCCCCCHHHHHHHHHHCCCEEEEEEE
TANVNALGKGFEGELSVADDRFIPGLSKLAAAMKTGGTKAILQIFSAGRMSNSKILRGEQ
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCC
PVSASAVAAPRAGYETPRALTSAEIEATIHDFGQAVRRAILAGFDGIELHGANTYLIQQF
CCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHH
YSPNSNRRTDEWGGDRDKRMRFPLAVVHEAEKVIATIADRPFLLGYRISPEELEQPGITL
HCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHCCCCCH
DDTLALIDTLKQTKIDYLHVSQSDVWRTSLRNPEDTAIMNEQIRDHVAGAFPVIVVGGIK
HHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEECCCC
TPADAEKAAESFDLVAIGHEMIREPHWVQKVLDHDEKAIRYQIAPADLEELGIAPTFLDF
CCCHHHHHHHHCCEEEECHHHHHCCHHHHHHHCCCCCEEEEEECCCCHHHHCCCHHHHHH
IESISGGAKGVPLTTAQSVTSSNVTQD
HHHHCCCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]