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The map label for this gene is mdtG [H]

Identifier: 116495726

GI number: 116495726

Start: 2272438

End: 2273670

Strand: Direct

Name: mdtG [H]

Synonym: LSEI_2274

Alternate gene names: 116495726

Gene position: 2272438-2273670 (Clockwise)

Preceding gene: 116495723

Following gene: 116495729

Centisome position: 78.49

GC content: 48.82

Gene sequence:

>1233_bases
TTGCCAAGTAAACTTAAAAGCCTCGAGCCATGGCAGCAAAATCTGGCTGTGTTATGGCTCGGAACCTTCATTGCCGGCAT
GGGCTTCAGTGAAGTTTCGCCATTTATTTCATTATATGTTGATCAACTTGGCGATTTTACCAAGGGGCAACTGAGTATTT
ACTCAGGGATCACGTTTGGGGTCACCTTCATGGTCGTTGCGATCGTGAGTCCGCTCTGGGGCCGACTCGCTGATCGCCGC
GGTCGAAAATTGATGTTGCTGCGTTCCAGTTTCGGAATGGCCATCATCATCGGAGCGTGTGGCTTTGTCCACAATGTCTA
CGCCTTAATTGCCTTACGTTTTCTGCAAGGGCTGGCAGCAGGTTACATTCCAAACGCCAGCGCCTTGATTGCAACCGAAA
CGCCAAAGGCAAAAAGCGGCACTGCGATTGGGATTCTCACAACCGGCTACGTTTCCGGTAACTTGATCGGTCCAATTCTC
GGCGGCTTTCTTGCCCAGACCTTTTCAATCCGGCTGACTTTCATCATCACCGGCGTTTTGCTGCTGATTGTGTTTGTCTT
GAGCCTAACGATGGTCCATGAGCATTACACACCGGATCCACGCTTGCTCAACAAGGAAAAACGCCCGAGTTTATTGTCTC
AAGTCAATAATCCAAAAGCTATCATGGTGCTATTAGTCTCCACCGCGGTGATTCAGCTAGGTAATATGTCCATTTATCCA
ATTATCAGCCTTTATGTCAAAGAGTTGATGCACAATGTTGGTCCAATTACCGTTGTTGCAGGCGTCATTGCAGCTTTGCC
AGGGATTTCCAACCTCATCGCGGCACCGCGCCTAGGTCAGCTTGGAGATCGCATCGGCTCAGGCAAGATTCTGGTCAGTG
GCTTGATTTTCGCGGTCATTATGTACATTCCTCAAGGCATTGTCGGCAGCATTTGGGCGCTCGGTTTCTTGCGTCTCATG
ATCGGCGTTTCGGATGGCGCGCTGTTTCCAACAATTCAAACGCTTCTGTCTAAGTTGACACCGGTGAGCTATACCGGCAC
CGTCTTCAGTTGGAATCAAAGTGCCCAAGCCTTTGGTTCCATGGCTGGCTCGCTAGCTGGCGGCTTCATCTCAAATATTT
TTAATTATAATGGTGTTTTTATTTTCACAGCCCTCTGTTTAGCGCTTAACCTAGTGATGGTGCTCGTCTTTATTCCACAC
ATCTGGTCAAGCCGACCAAAACAACAAGCATAA

Upstream 100 bases:

>100_bases
TCCATTTCTGACTAATAGGCTCCTTGCTATCGAATTGTAAGCCCCCTCTGATTGAGGTATATTAATTAGGTAACTATTTT
CTGAAAGGAAGTTGGCGCTC

Downstream 100 bases:

>100_bases
CGCATCAAAAAAACCGACCTGCGTGGGCAGATCGGTTTTTTATTGTCTAAATTAAATCACGGCATCCGCGTGGCATCAAG
CAATAGCTGCCAACTGATCA

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MPSKLKSLEPWQQNLAVLWLGTFIAGMGFSEVSPFISLYVDQLGDFTKGQLSIYSGITFGVTFMVVAIVSPLWGRLADRR
GRKLMLLRSSFGMAIIIGACGFVHNVYALIALRFLQGLAAGYIPNASALIATETPKAKSGTAIGILTTGYVSGNLIGPIL
GGFLAQTFSIRLTFIITGVLLLIVFVLSLTMVHEHYTPDPRLLNKEKRPSLLSQVNNPKAIMVLLVSTAVIQLGNMSIYP
IISLYVKELMHNVGPITVVAGVIAALPGISNLIAAPRLGQLGDRIGSGKILVSGLIFAVIMYIPQGIVGSIWALGFLRLM
IGVSDGALFPTIQTLLSKLTPVSYTGTVFSWNQSAQAFGSMAGSLAGGFISNIFNYNGVFIFTALCLALNLVMVLVFIPH
IWSSRPKQQA

Sequences:

>Translated_410_residues
MPSKLKSLEPWQQNLAVLWLGTFIAGMGFSEVSPFISLYVDQLGDFTKGQLSIYSGITFGVTFMVVAIVSPLWGRLADRR
GRKLMLLRSSFGMAIIIGACGFVHNVYALIALRFLQGLAAGYIPNASALIATETPKAKSGTAIGILTTGYVSGNLIGPIL
GGFLAQTFSIRLTFIITGVLLLIVFVLSLTMVHEHYTPDPRLLNKEKRPSLLSQVNNPKAIMVLLVSTAVIQLGNMSIYP
IISLYVKELMHNVGPITVVAGVIAALPGISNLIAAPRLGQLGDRIGSGKILVSGLIFAVIMYIPQGIVGSIWALGFLRLM
IGVSDGALFPTIQTLLSKLTPVSYTGTVFSWNQSAQAFGSMAGSLAGGFISNIFNYNGVFIFTALCLALNLVMVLVFIPH
IWSSRPKQQA
>Mature_409_residues
PSKLKSLEPWQQNLAVLWLGTFIAGMGFSEVSPFISLYVDQLGDFTKGQLSIYSGITFGVTFMVVAIVSPLWGRLADRRG
RKLMLLRSSFGMAIIIGACGFVHNVYALIALRFLQGLAAGYIPNASALIATETPKAKSGTAIGILTTGYVSGNLIGPILG
GFLAQTFSIRLTFIITGVLLLIVFVLSLTMVHEHYTPDPRLLNKEKRPSLLSQVNNPKAIMVLLVSTAVIQLGNMSIYPI
ISLYVKELMHNVGPITVVAGVIAALPGISNLIAAPRLGQLGDRIGSGKILVSGLIFAVIMYIPQGIVGSIWALGFLRLMI
GVSDGALFPTIQTLLSKLTPVSYTGTVFSWNQSAQAFGSMAGSLAGGFISNIFNYNGVFIFTALCLALNLVMVLVFIPHI
WSSRPKQQA

Specific function: Confers resistance to fosfomycin and deoxycholate [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtG (TC 2.A.1.2.20) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=399, Percent_Identity=43.609022556391, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI87082404, Length=298, Percent_Identity=26.1744966442953, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43939; Mature: 43808

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKLKSLEPWQQNLAVLWLGTFIAGMGFSEVSPFISLYVDQLGDFTKGQLSIYSGITFG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHH
VTFMVVAIVSPLWGRLADRRGRKLMLLRSSFGMAIIIGACGFVHNVYALIALRFLQGLAA
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYIPNASALIATETPKAKSGTAIGILTTGYVSGNLIGPILGGFLAQTFSIRLTFIITGVL
CCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIVFVLSLTMVHEHYTPDPRLLNKEKRPSLLSQVNNPKAIMVLLVSTAVIQLGNMSIYP
HHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHH
IISLYVKELMHNVGPITVVAGVIAALPGISNLIAAPRLGQLGDRIGSGKILVSGLIFAVI
HHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
MYIPQGIVGSIWALGFLRLMIGVSDGALFPTIQTLLSKLTPVSYTGTVFSWNQSAQAFGS
HHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHH
MAGSLAGGFISNIFNYNGVFIFTALCLALNLVMVLVFIPHIWSSRPKQQA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PSKLKSLEPWQQNLAVLWLGTFIAGMGFSEVSPFISLYVDQLGDFTKGQLSIYSGITFG
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHH
VTFMVVAIVSPLWGRLADRRGRKLMLLRSSFGMAIIIGACGFVHNVYALIALRFLQGLAA
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYIPNASALIATETPKAKSGTAIGILTTGYVSGNLIGPILGGFLAQTFSIRLTFIITGVL
CCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLIVFVLSLTMVHEHYTPDPRLLNKEKRPSLLSQVNNPKAIMVLLVSTAVIQLGNMSIYP
HHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHH
IISLYVKELMHNVGPITVVAGVIAALPGISNLIAAPRLGQLGDRIGSGKILVSGLIFAVI
HHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
MYIPQGIVGSIWALGFLRLMIGVSDGALFPTIQTLLSKLTPVSYTGTVFSWNQSAQAFGS
HHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHH
MAGSLAGGFISNIFNYNGVFIFTALCLALNLVMVLVFIPHIWSSRPKQQA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA