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The map label for this gene is ilvE [H]

Identifier: 116495504

GI number: 116495504

Start: 2023934

End: 2024965

Strand: Direct

Name: ilvE [H]

Synonym: LSEI_2041

Alternate gene names: 116495504

Gene position: 2023934-2024965 (Clockwise)

Preceding gene: 116495497

Following gene: 116495507

Centisome position: 69.9

GC content: 49.22

Gene sequence:

>1032_bases
ATGAGTGTCAATATTGATTGGAACAATCTAGGCTTCGATTATATGCAACTGCCATACCGTTATGTTGCCCACTGGAAAGA
CGGGGCATGGGATGAAGGTAAGCTGTCCACCGATCCCAACCTGACGATGAATGAAGGCTCCCCGATTTTGCATTATGGTC
AAGGCGCTTTTGAAGGTATGAAGGCATACCGGACCAAATCAGGAAAAATCCAACTTTTCCGGCCTGACCAAAATGCCCAT
CGACTGCACAACTCTGCTGACAAGCTACTGATGCCGCCATTTCCTGAAGATCGCTTTATTGATGCGGTCAAGCAAGTCGT
TGCCGCTAATCATGAATACGTACCGCCATATGGCACCGGTGCTACCTTATACTTACGCCCGATTTTGATTGGTGTCGGTC
CCAATATCGGGGTTGCTCCGGCTAAGGAATACATTTTCGATGTCTTCGCCATGCCAGTTGGCCCCTACTTCAAAGGCGGC
ATGGTGCCAACCAAGTTCATCGTTGCCGATCAATTCGACCGGGCGGCACATTACGGCACTGGCCAATCGAAAGTCGGCGG
GAACTACGCCGCATCCCTGCAAGCCGGCAAATTCGCCCACGAGCACGGTTATGGCGATGCGATCTATCTGGACCCAATTG
AACATAAATATATTGAAGAAGTTGGGTCAGCTAACTTCTTCGGCATTAGCAAAGATGGTAAAACTTTGAAAACACCGAAG
TCACCATCCATCCTGCCAAGTATCACCAAGTATTCAATTTTGGCACTGGCGCATGATCGGTTTGGCATGACCACTGAAGA
AACTAAGATTGCGATTACCGATTTGGATCAATTCGGTGAAGCCGGGGCCTGTGGCACTGCGGCGGTCATCACCCCGATTG
CCAGCATCACCTACGAAGACCACGAACATGTCTTTTATTCGGAAACCAAAGTCGGTCCATATACACAAAAACTGTATGAT
GAACTGACGGGTATCCAGTTTGGCGATGTACCGGCACCTGAAGGCTGGGTCGTTGACGTCCCGTTTAATTAA

Upstream 100 bases:

>100_bases
TATTATTTAATGAAACCGCAATGTTCACGCAACGTTACTCTGAAATCAACCTCAGCCGGCTGTTTTCAGTCAGACGCACA
CAAATACTGGAGGTACTGCC

Downstream 100 bases:

>100_bases
TTTAAAAATCATTTATCACTCGGAAACTGTGAGAACTTGCACGCGCTATAACGCATCTGCAATCGCGTACGCCCGCTCAC
GCTTCCACAAAAAAAGCAGC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSVNIDWNNLGFDYMQLPYRYVAHWKDGAWDEGKLSTDPNLTMNEGSPILHYGQGAFEGMKAYRTKSGKIQLFRPDQNAH
RLHNSADKLLMPPFPEDRFIDAVKQVVAANHEYVPPYGTGATLYLRPILIGVGPNIGVAPAKEYIFDVFAMPVGPYFKGG
MVPTKFIVADQFDRAAHYGTGQSKVGGNYAASLQAGKFAHEHGYGDAIYLDPIEHKYIEEVGSANFFGISKDGKTLKTPK
SPSILPSITKYSILALAHDRFGMTTEETKIAITDLDQFGEAGACGTAAVITPIASITYEDHEHVFYSETKVGPYTQKLYD
ELTGIQFGDVPAPEGWVVDVPFN

Sequences:

>Translated_343_residues
MSVNIDWNNLGFDYMQLPYRYVAHWKDGAWDEGKLSTDPNLTMNEGSPILHYGQGAFEGMKAYRTKSGKIQLFRPDQNAH
RLHNSADKLLMPPFPEDRFIDAVKQVVAANHEYVPPYGTGATLYLRPILIGVGPNIGVAPAKEYIFDVFAMPVGPYFKGG
MVPTKFIVADQFDRAAHYGTGQSKVGGNYAASLQAGKFAHEHGYGDAIYLDPIEHKYIEEVGSANFFGISKDGKTLKTPK
SPSILPSITKYSILALAHDRFGMTTEETKIAITDLDQFGEAGACGTAAVITPIASITYEDHEHVFYSETKVGPYTQKLYD
ELTGIQFGDVPAPEGWVVDVPFN
>Mature_342_residues
SVNIDWNNLGFDYMQLPYRYVAHWKDGAWDEGKLSTDPNLTMNEGSPILHYGQGAFEGMKAYRTKSGKIQLFRPDQNAHR
LHNSADKLLMPPFPEDRFIDAVKQVVAANHEYVPPYGTGATLYLRPILIGVGPNIGVAPAKEYIFDVFAMPVGPYFKGGM
VPTKFIVADQFDRAAHYGTGQSKVGGNYAASLQAGKFAHEHGYGDAIYLDPIEHKYIEEVGSANFFGISKDGKTLKTPKS
PSILPSITKYSILALAHDRFGMTTEETKIAITDLDQFGEAGACGTAAVITPIASITYEDHEHVFYSETKVGPYTQKLYDE
LTGIQFGDVPAPEGWVVDVPFN

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=336, Percent_Identity=33.9285714285714, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI38176287, Length=336, Percent_Identity=33.9285714285714, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI296010904, Length=336, Percent_Identity=33.9285714285714, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI50658084, Length=320, Percent_Identity=31.5625, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI258614015, Length=285, Percent_Identity=31.9298245614035, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI296010902, Length=270, Percent_Identity=33.7037037037037, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI296010900, Length=270, Percent_Identity=33.7037037037037, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI48994963, Length=320, Percent_Identity=29.6875, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17568601, Length=355, Percent_Identity=30.4225352112676, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17565728, Length=342, Percent_Identity=30.1169590643275, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322608, Length=332, Percent_Identity=31.9277108433735, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322002, Length=307, Percent_Identity=31.5960912052117, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24641779, Length=327, Percent_Identity=32.7217125382263, Blast_Score=165, Evalue=4e-41,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37800; Mature: 37669

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNIDWNNLGFDYMQLPYRYVAHWKDGAWDEGKLSTDPNLTMNEGSPILHYGQGAFEGM
CEEEEECCCCCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHH
KAYRTKSGKIQLFRPDQNAHRLHNSADKLLMPPFPEDRFIDAVKQVVAANHEYVPPYGTG
HHHHCCCCCEEEECCCCHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPILIGVGPNIGVAPAKEYIFDVFAMPVGPYFKGGMVPTKFIVADQFDRAAHYGT
CEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHCCC
GQSKVGGNYAASLQAGKFAHEHGYGDAIYLDPIEHKYIEEVGSANFFGISKDGKTLKTPK
CCCCCCCCEEEECCCCCCHHHCCCCCEEEECCHHHHHHHHHCCCCEEEECCCCCEECCCC
SPSILPSITKYSILALAHDRFGMTTEETKIAITDLDQFGEAGACGTAAVITPIASITYED
CCCCCCCHHHHEEEEEEHHHCCCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHEEECC
HEHVFYSETKVGPYTQKLYDELTGIQFGDVPAPEGWVVDVPFN
CCEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEEECCCC
>Mature Secondary Structure 
SVNIDWNNLGFDYMQLPYRYVAHWKDGAWDEGKLSTDPNLTMNEGSPILHYGQGAFEGM
EEEEECCCCCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHH
KAYRTKSGKIQLFRPDQNAHRLHNSADKLLMPPFPEDRFIDAVKQVVAANHEYVPPYGTG
HHHHCCCCCEEEECCCCHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
ATLYLRPILIGVGPNIGVAPAKEYIFDVFAMPVGPYFKGGMVPTKFIVADQFDRAAHYGT
CEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHCCC
GQSKVGGNYAASLQAGKFAHEHGYGDAIYLDPIEHKYIEEVGSANFFGISKDGKTLKTPK
CCCCCCCCEEEECCCCCCHHHCCCCCEEEECCHHHHHHHHHCCCCEEEECCCCCEECCCC
SPSILPSITKYSILALAHDRFGMTTEETKIAITDLDQFGEAGACGTAAVITPIASITYED
CCCCCCCHHHHEEEEEEHHHCCCCCCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHEEECC
HEHVFYSETKVGPYTQKLYDELTGIQFGDVPAPEGWVVDVPFN
CCEEEEECCCCCHHHHHHHHHHHCCEECCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]