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The map label for this gene is apbE [H]

Identifier: 116495497

GI number: 116495497

Start: 2011686

End: 2012615

Strand: Direct

Name: apbE [H]

Synonym: LSEI_2034

Alternate gene names: 116495497

Gene position: 2011686-2012615 (Clockwise)

Preceding gene: 116495493

Following gene: 116495504

Centisome position: 69.48

GC content: 51.4

Gene sequence:

>930_bases
ATGACCACGACGACCACTTTTCATGGTTTGGGTGCGGATAACACGCTTACCGTTTATGCAGATGGTGCTAATGAACTTTT
GAATCAAAGCATCAGTATGATCAATGCTTATGCCCATTTATTATCGCTGTATGATGAAGCATCCCTGCTGACTTTGATCA
ACAAGCAGGCCGGCAAAGAACCGGTTCACATTCCGGTTCGCCCTGTGTTCAATCTGATTGCTCAAGCGATCGCCTGGAGC
AAACGCGGATTAGGTTACAACGCCTTAATCGGCCCGATCGTCAAACTTTGGCGCATCGGTTTCGATGATGCCCATGTTCC
GACCGCCGAAGAAGTCACTGCCGCGCTTGCTTTGACTGATCCCGATCTTGTCGAGCTTGATTCAGAAAATCAAACTGTTT
TTCTGACCAAACCCGGCATGGCCCTTGATCTTGGCGGTATCGCCAAAGGTTTCATTGTCGATCAAGTCTATGATCTTTGG
GACAAAACCGGCGTTGAAGGTGGGCTGATTGACTTAGGTGGTAACCAGCGGCTCGTCGGTAATGCAGCCAGCGGCGTACC
GTTATGGCAGTGGCCAATCACTGACCCGCGGACACCGGGAAAACGCAGTATCGCCACCTTGACAACCACCCCTAAAGCCG
TCGTGACGACTGGTATTTATGAGCGGCACTCAGAGATCAACGGCAAAGAATATCACCATTTGCTTGATCCTAACACAGGC
TATCCCGTCGAATCAGACATGGCCAGCATTACCGTCGTGGCCGATAGTGGCCTGATCGGAGACGTGTTATCTTCCATCGG
TTTTTATGCCGGCATCCCTGCCGGTTACGATGCCGTTGAAGCCGAGGGTGAGGAGGCCATCTTCGTTACTAAAGATACCC
GCGTTTACCAAACCAGCGGCCTGCGCGAAACCTTGGCCGTCACCATGTAA

Upstream 100 bases:

>100_bases
CACTCATAACGAATATTACCTGAAATCGTAACGATTGCAACCAATATGCAAACCGTTCATACTGAATGCATGTCAATCGA
TTATTTTTGGAGGTGCCAAT

Downstream 100 bases:

>100_bases
ACCTTAGCCACCGTTGTCTTCAGACATAATAGTTTGGGACATCACTCATAATCATCAAAAAGGCAAACAAATTCCACTGT
AAAGCGGAAACTGTTTGCCT

Product: thiamine biosynthesis membrane-associated lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTTTTTFHGLGADNTLTVYADGANELLNQSISMINAYAHLLSLYDEASLLTLINKQAGKEPVHIPVRPVFNLIAQAIAWS
KRGLGYNALIGPIVKLWRIGFDDAHVPTAEEVTAALALTDPDLVELDSENQTVFLTKPGMALDLGGIAKGFIVDQVYDLW
DKTGVEGGLIDLGGNQRLVGNAASGVPLWQWPITDPRTPGKRSIATLTTTPKAVVTTGIYERHSEINGKEYHHLLDPNTG
YPVESDMASITVVADSGLIGDVLSSIGFYAGIPAGYDAVEAEGEEAIFVTKDTRVYQTSGLRETLAVTM

Sequences:

>Translated_309_residues
MTTTTTFHGLGADNTLTVYADGANELLNQSISMINAYAHLLSLYDEASLLTLINKQAGKEPVHIPVRPVFNLIAQAIAWS
KRGLGYNALIGPIVKLWRIGFDDAHVPTAEEVTAALALTDPDLVELDSENQTVFLTKPGMALDLGGIAKGFIVDQVYDLW
DKTGVEGGLIDLGGNQRLVGNAASGVPLWQWPITDPRTPGKRSIATLTTTPKAVVTTGIYERHSEINGKEYHHLLDPNTG
YPVESDMASITVVADSGLIGDVLSSIGFYAGIPAGYDAVEAEGEEAIFVTKDTRVYQTSGLRETLAVTM
>Mature_308_residues
TTTTTFHGLGADNTLTVYADGANELLNQSISMINAYAHLLSLYDEASLLTLINKQAGKEPVHIPVRPVFNLIAQAIAWSK
RGLGYNALIGPIVKLWRIGFDDAHVPTAEEVTAALALTDPDLVELDSENQTVFLTKPGMALDLGGIAKGFIVDQVYDLWD
KTGVEGGLIDLGGNQRLVGNAASGVPLWQWPITDPRTPGKRSIATLTTTPKAVVTTGIYERHSEINGKEYHHLLDPNTGY
PVESDMASITVVADSGLIGDVLSSIGFYAGIPAGYDAVEAEGEEAIFVTKDTRVYQTSGLRETLAVTM

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=288, Percent_Identity=25.3472222222222, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 33126; Mature: 32995

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTTFHGLGADNTLTVYADGANELLNQSISMINAYAHLLSLYDEASLLTLINKQAGKE
CCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
PVHIPVRPVFNLIAQAIAWSKRGLGYNALIGPIVKLWRIGFDDAHVPTAEEVTAALALTD
CEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEECC
PDLVELDSENQTVFLTKPGMALDLGGIAKGFIVDQVYDLWDKTGVEGGLIDLGGNQRLVG
CCEEEECCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEC
NAASGVPLWQWPITDPRTPGKRSIATLTTTPKAVVTTGIYERHSEINGKEYHHLLDPNTG
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHEEECCCCCC
YPVESDMASITVVADSGLIGDVLSSIGFYAGIPAGYDAVEAEGEEAIFVTKDTRVYQTSG
CCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCEEEECCC
LRETLAVTM
CHHHHEECC
>Mature Secondary Structure 
TTTTTFHGLGADNTLTVYADGANELLNQSISMINAYAHLLSLYDEASLLTLINKQAGKE
CCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
PVHIPVRPVFNLIAQAIAWSKRGLGYNALIGPIVKLWRIGFDDAHVPTAEEVTAALALTD
CEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEEECC
PDLVELDSENQTVFLTKPGMALDLGGIAKGFIVDQVYDLWDKTGVEGGLIDLGGNQRLVG
CCEEEECCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEC
NAASGVPLWQWPITDPRTPGKRSIATLTTTPKAVVTTGIYERHSEINGKEYHHLLDPNTG
CCCCCCEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHEEECCCCCC
YPVESDMASITVVADSGLIGDVLSSIGFYAGIPAGYDAVEAEGEEAIFVTKDTRVYQTSG
CCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCEEEECCC
LRETLAVTM
CHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]