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The map label for this gene is lplA [H]

Identifier: 116494959

GI number: 116494959

Start: 1467379

End: 1468392

Strand: Direct

Name: lplA [H]

Synonym: LSEI_1473

Alternate gene names: 116494959

Gene position: 1467379-1468392 (Clockwise)

Preceding gene: 116494956

Following gene: 116494965

Centisome position: 50.68

GC content: 43.29

Gene sequence:

>1014_bases
ATGTATTATGTCATTATGAAGTCTCATGATATTCGTGAGAATCTGGCAACAGAACAATATCTCATGAACTCGAAACAATT
TGATGAACCGTTACTGCTGTTCTACTATGAAAAGCCGAGTGTGATTGTGGGCCGAAACCAGAATACGCTTGAAGAGTTGA
ATCAAAAATACGTGGAGGATCATAACATTGTTGTGACGCGGCGGTTATCCGGTGGCGGCGCAGTTTATCATGATCTCAAT
AACTTGTGCTTCTCGTTTGTGGTTGACAGTGACTCTGAAGAATTTGGTGACTTCAAGTCCTTCACACAGCCGATTGTTGA
TGCAATTCATACTTTAGGGGCGACTAGCGCTGAAGTTTCTGGCCGTAATGACATGCTGGTCGACGGTAAAAAGTTCTCTG
GCAGTGCTATGTATACACGTAACGGCAAGACGTTCTCCCATGGTACTTTGATGCTGGATGTTGACATGAGTGTGATTCCT
AACGTCTTGAACGTCCCAGAAGACAAGATCAAGAGTAAGGGCATTAAATCTGTTAAGAGCCGTGTGACGAACTTGCGGCC
TTATCTAGATAAAAAGTATCAAAACCTCACTGTTCCTGAATTCCGCGATATTCTTTTGACTCGGTTGTTCGGCGTTGATG
ACATCGCTGAAATCAAAGACAAAGAGTATCACGTCACCCCAGAAGACGAAGTCGAAATTAAAAAGATTTACGATGATGTT
TACAACAACTGGGATTGGGTATATGGCCATAGCCCGGAATTCACCACCAAGAAGCGGAAGCATTTTGACTATGGGACGAT
TGATGCTCGTTTCGATGTCAAGGAAGGCAAAATTGCTAACGTCAAATTCTATGGTGATTTCTTTGGTCCTAAGGATGTTG
CGGATGTTGCCGGTGCGTTAAAAGGCAAGCCTTACACGTCAGCAGCCATTAAAGACACTTTAGATGCCATCAACACGAAT
GAGTACTTCACCAATATTCCTAAGGACGATGTGGTTAACCTGCTCGTACCATAA

Upstream 100 bases:

>100_bases
CGAAATCGCTTTCTTTTTAATGATTAACCCGCTAAAATGGAGCATGAAGTCAAATCGATGGCTAGTCGTCAATGGCAGTC
ATCATTCAGGAGGATTGAAA

Downstream 100 bases:

>100_bases
TCGCATCAGTTTTTAGAACTTAAGGCAGCACGGCCAAATGCTGTGCCGCCTTTTTTCATGCAAAAAGGACAACCTTTAAC
AGGCTGTCCTTCGATCGATC

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MYYVIMKSHDIRENLATEQYLMNSKQFDEPLLLFYYEKPSVIVGRNQNTLEELNQKYVEDHNIVVTRRLSGGGAVYHDLN
NLCFSFVVDSDSEEFGDFKSFTQPIVDAIHTLGATSAEVSGRNDMLVDGKKFSGSAMYTRNGKTFSHGTLMLDVDMSVIP
NVLNVPEDKIKSKGIKSVKSRVTNLRPYLDKKYQNLTVPEFRDILLTRLFGVDDIAEIKDKEYHVTPEDEVEIKKIYDDV
YNNWDWVYGHSPEFTTKKRKHFDYGTIDARFDVKEGKIANVKFYGDFFGPKDVADVAGALKGKPYTSAAIKDTLDAINTN
EYFTNIPKDDVVNLLVP

Sequences:

>Translated_337_residues
MYYVIMKSHDIRENLATEQYLMNSKQFDEPLLLFYYEKPSVIVGRNQNTLEELNQKYVEDHNIVVTRRLSGGGAVYHDLN
NLCFSFVVDSDSEEFGDFKSFTQPIVDAIHTLGATSAEVSGRNDMLVDGKKFSGSAMYTRNGKTFSHGTLMLDVDMSVIP
NVLNVPEDKIKSKGIKSVKSRVTNLRPYLDKKYQNLTVPEFRDILLTRLFGVDDIAEIKDKEYHVTPEDEVEIKKIYDDV
YNNWDWVYGHSPEFTTKKRKHFDYGTIDARFDVKEGKIANVKFYGDFFGPKDVADVAGALKGKPYTSAAIKDTLDAINTN
EYFTNIPKDDVVNLLVP
>Mature_337_residues
MYYVIMKSHDIRENLATEQYLMNSKQFDEPLLLFYYEKPSVIVGRNQNTLEELNQKYVEDHNIVVTRRLSGGGAVYHDLN
NLCFSFVVDSDSEEFGDFKSFTQPIVDAIHTLGATSAEVSGRNDMLVDGKKFSGSAMYTRNGKTFSHGTLMLDVDMSVIP
NVLNVPEDKIKSKGIKSVKSRVTNLRPYLDKKYQNLTVPEFRDILLTRLFGVDDIAEIKDKEYHVTPEDEVEIKKIYDDV
YNNWDWVYGHSPEFTTKKRKHFDYGTIDARFDVKEGKIANVKFYGDFFGPKDVADVAGALKGKPYTSAAIKDTLDAINTN
EYFTNIPKDDVVNLLVP

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes [H]

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=338, Percent_Identity=27.2189349112426, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21729884, Length=338, Percent_Identity=27.2189349112426, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21729882, Length=338, Percent_Identity=27.2189349112426, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21729880, Length=338, Percent_Identity=27.2189349112426, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI21729878, Length=338, Percent_Identity=27.2189349112426, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1790846, Length=323, Percent_Identity=31.2693498452012, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI71981909, Length=252, Percent_Identity=30.952380952381, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322415, Length=181, Percent_Identity=32.0441988950276, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28573464, Length=325, Percent_Identity=28.6153846153846, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 38444; Mature: 38444

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYVIMKSHDIRENLATEQYLMNSKQFDEPLLLFYYEKPSVIVGRNQNTLEELNQKYVED
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHCC
HNIVVTRRLSGGGAVYHDLNNLCFSFVVDSDSEEFGDFKSFTQPIVDAIHTLGATSAEVS
CCEEEEEEECCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GRNDMLVDGKKFSGSAMYTRNGKTFSHGTLMLDVDMSVIPNVLNVPEDKIKSKGIKSVKS
CCCCEEECCCCCCCCEEEEECCCEEECCEEEEEECHHHCCHHHCCCHHHHHHHHHHHHHH
RVTNLRPYLDKKYQNLTVPEFRDILLTRLFGVDDIAEIKDKEYHVTPEDEVEIKKIYDDV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCCEECCCCCCHHHHHHHHHH
YNNWDWVYGHSPEFTTKKRKHFDYGTIDARFDVKEGKIANVKFYGDFFGPKDVADVAGAL
HCCCCEEECCCCCCCCHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHH
KGKPYTSAAIKDTLDAINTNEYFTNIPKDDVVNLLVP
CCCCCCHHHHHHHHHHCCCCHHHCCCCHHHHHEECCC
>Mature Secondary Structure
MYYVIMKSHDIRENLATEQYLMNSKQFDEPLLLFYYEKPSVIVGRNQNTLEELNQKYVED
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHCC
HNIVVTRRLSGGGAVYHDLNNLCFSFVVDSDSEEFGDFKSFTQPIVDAIHTLGATSAEVS
CCEEEEEEECCCCHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GRNDMLVDGKKFSGSAMYTRNGKTFSHGTLMLDVDMSVIPNVLNVPEDKIKSKGIKSVKS
CCCCEEECCCCCCCCEEEEECCCEEECCEEEEEECHHHCCHHHCCCHHHHHHHHHHHHHH
RVTNLRPYLDKKYQNLTVPEFRDILLTRLFGVDDIAEIKDKEYHVTPEDEVEIKKIYDDV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCCEECCCCCCHHHHHHHHHH
YNNWDWVYGHSPEFTTKKRKHFDYGTIDARFDVKEGKIANVKFYGDFFGPKDVADVAGAL
HCCCCEEECCCCCCCCHHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHH
KGKPYTSAAIKDTLDAINTNEYFTNIPKDDVVNLLVP
CCCCCCHHHHHHHHHHCCCCHHHCCCCHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]