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The map label for this gene is ywnE [H]

Identifier: 116494754

GI number: 116494754

Start: 1241818

End: 1243251

Strand: Direct

Name: ywnE [H]

Synonym: LSEI_1263

Alternate gene names: 116494754

Gene position: 1241818-1243251 (Clockwise)

Preceding gene: 116494753

Following gene: 116494756

Centisome position: 42.89

GC content: 47.35

Gene sequence:

>1434_bases
ATGTGGATCTGGTTGGTCCTTGGGTTTCTTTACATCATCAACGCACTGACAGCGATCATCACGGTCTTTCGTTCGCATCG
TGATATTTCTGCAGTTTGGGCATGGCTATTGGTGCTTTTACTGTTGCCGGTGGTCGGCTTCTTGATCTATTTTGTTTTTG
GCCGCAAGTTACGGGCTAATCGCATGCAGGATCTGATGACGCAGCGTTTGCTGGGCATCGACGATTTAGTTGATCAGCAG
CAGCAAGCCCTGGCAGCTGGCCAACCGTTGATTGGAACGCCTGGCAGTGGCGGTGTTTCCGAACTAGTCCGAACCTTATT
GCGTGCTGATGACGCGCTAGTAACCACCCACAATGATGTGCAAGTCTTAGCATTGCGTGAAGCCTTTTTTAAGCAATTGT
TCGAAGATATTGCTGTGGCAACGGAACATATTCACATCGAAGCTTATACTATTCGGCCTGATGCAATTGGCCACCAGTTG
CGCGACTTGTTAATTGAAAAAGCCCACGCCGGTGTCACCGTACGCGTGCTTTACGATACATTTGGCTCCCACGACCTACC
AAACAATTTTTGGAAAAAATTAACGGCAGCTGGCGGTCAGGTTGAGCGGTTTGTCGCTACGAAACTGGGGCGTTGGAATC
CGCGAATCAACTTTCGTAATCATCGTAAGCTGATCATTATTGACGAACAGCTTGCCTATCTGGGCGGTTTTAACCTCGGC
AAGGAACACCACGGATTGCCAATGTTGCGTGATACTCAGTTACGAATTCAGGGCAAGGCGGTCGCCGTGGCCCAGGCGCG
CTTTTTGATGGACTGGAACGGAACGAGCAAGCAACAAAAAGTAAAAAATGTTGATGTCCTGTTTAGAGAACCGGTTGTCA
CTGGGAACACGACGATGCAAATTGTCTCTGGCGGACCAGAGCAGCAGGTTGAAGCAATCAAACTAGGCTATCTTGGCCTC
ATTAATCAGGCAAAAAGAAGTATTTGGATCGAATCGCCATATTTCGTCCCAGATGACAGTTTGCTGGATGCGCTAACCAT
TGCGGCGAATGCCGGCGTGGATGTTCGAGTGATGATTCCCAAAAAAGCCAATCAGATCTTGATGGCCAAGGCTTCGCTAT
TTTATCTTAATCAGGTTGTGGCAAATGGTGGACAGGCATTTTTATATGAAGAAGGTTTTTTACATGCTAAAACGATTGTG
GTTGACGGCAAATACGTTTCAACTGGCACTGCCAACTTCGATATCCGTTCGTTTAAACTGAACTTTGAAATTGCGGCATT
TATTTACGACCATCGGATTGGGAAGACTTTCCAAAAGCTGTTCTTAGCGGACATGGAACGGGCAATGCCTTACACGCAAA
AAGTGATCAAAGCAAAAACGCGGCCTAAGCAACTCAGTGAAGAACTCTCCCGCTTACTAGCACCAATTCTGTAA

Upstream 100 bases:

>100_bases
GGCATAGCTGGGCTAATTAGGTTATGATACTTAAGTAACCTTCGCCAATGAAACCACTTGTAGGCTCAAAATTTGCTGGC
AGTTACGGGGGGAAAATGTT

Downstream 100 bases:

>100_bases
TCGCCGCGCCATGAAGCTGAGTTGAAAAAGGGCAGATAAAAAGCCGCTGGCACACAGCGGCTTTAAGTATAAGGTGAGAT
CGGTTTAGATCAATTGTAAA

Product: phosphatidylserine/phosphatidylglycerophosphate/ cardiolipin synthase-like protein

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MWIWLVLGFLYIINALTAIITVFRSHRDISAVWAWLLVLLLLPVVGFLIYFVFGRKLRANRMQDLMTQRLLGIDDLVDQQ
QQALAAGQPLIGTPGSGGVSELVRTLLRADDALVTTHNDVQVLALREAFFKQLFEDIAVATEHIHIEAYTIRPDAIGHQL
RDLLIEKAHAGVTVRVLYDTFGSHDLPNNFWKKLTAAGGQVERFVATKLGRWNPRINFRNHRKLIIIDEQLAYLGGFNLG
KEHHGLPMLRDTQLRIQGKAVAVAQARFLMDWNGTSKQQKVKNVDVLFREPVVTGNTTMQIVSGGPEQQVEAIKLGYLGL
INQAKRSIWIESPYFVPDDSLLDALTIAANAGVDVRVMIPKKANQILMAKASLFYLNQVVANGGQAFLYEEGFLHAKTIV
VDGKYVSTGTANFDIRSFKLNFEIAAFIYDHRIGKTFQKLFLADMERAMPYTQKVIKAKTRPKQLSEELSRLLAPIL

Sequences:

>Translated_477_residues
MWIWLVLGFLYIINALTAIITVFRSHRDISAVWAWLLVLLLLPVVGFLIYFVFGRKLRANRMQDLMTQRLLGIDDLVDQQ
QQALAAGQPLIGTPGSGGVSELVRTLLRADDALVTTHNDVQVLALREAFFKQLFEDIAVATEHIHIEAYTIRPDAIGHQL
RDLLIEKAHAGVTVRVLYDTFGSHDLPNNFWKKLTAAGGQVERFVATKLGRWNPRINFRNHRKLIIIDEQLAYLGGFNLG
KEHHGLPMLRDTQLRIQGKAVAVAQARFLMDWNGTSKQQKVKNVDVLFREPVVTGNTTMQIVSGGPEQQVEAIKLGYLGL
INQAKRSIWIESPYFVPDDSLLDALTIAANAGVDVRVMIPKKANQILMAKASLFYLNQVVANGGQAFLYEEGFLHAKTIV
VDGKYVSTGTANFDIRSFKLNFEIAAFIYDHRIGKTFQKLFLADMERAMPYTQKVIKAKTRPKQLSEELSRLLAPIL
>Mature_477_residues
MWIWLVLGFLYIINALTAIITVFRSHRDISAVWAWLLVLLLLPVVGFLIYFVFGRKLRANRMQDLMTQRLLGIDDLVDQQ
QQALAAGQPLIGTPGSGGVSELVRTLLRADDALVTTHNDVQVLALREAFFKQLFEDIAVATEHIHIEAYTIRPDAIGHQL
RDLLIEKAHAGVTVRVLYDTFGSHDLPNNFWKKLTAAGGQVERFVATKLGRWNPRINFRNHRKLIIIDEQLAYLGGFNLG
KEHHGLPMLRDTQLRIQGKAVAVAQARFLMDWNGTSKQQKVKNVDVLFREPVVTGNTTMQIVSGGPEQQVEAIKLGYLGL
INQAKRSIWIESPYFVPDDSLLDALTIAANAGVDVRVMIPKKANQILMAKASLFYLNQVVANGGQAFLYEEGFLHAKTIV
VDGKYVSTGTANFDIRSFKLNFEIAAFIYDHRIGKTFQKLFLADMERAMPYTQKVIKAKTRPKQLSEELSRLLAPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=487, Percent_Identity=26.4887063655031, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1787007, Length=326, Percent_Identity=29.7546012269939, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53656; Mature: 53656

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIWLVLGFLYIINALTAIITVFRSHRDISAVWAWLLVLLLLPVVGFLIYFVFGRKLRAN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RMQDLMTQRLLGIDDLVDQQQQALAAGQPLIGTPGSGGVSELVRTLLRADDALVTTHNDV
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCE
QVLALREAFFKQLFEDIAVATEHIHIEAYTIRPDAIGHQLRDLLIEKAHAGVTVRVLYDT
EEHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEC
FGSHDLPNNFWKKLTAAGGQVERFVATKLGRWNPRINFRNHRKLIIIDEQLAYLGGFNLG
CCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCC
KEHHGLPMLRDTQLRIQGKAVAVAQARFLMDWNGTSKQQKVKNVDVLFREPVVTGNTTMQ
CCCCCCCCEECCEEEEECCEEEEEHEEEEEECCCCCHHHHHHCHHEEEECCEECCCCEEE
IVSGGPEQQVEAIKLGYLGLINQAKRSIWIESPYFVPDDSLLDALTIAANAGVDVRVMIP
EECCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
KKANQILMAKASLFYLNQVVANGGQAFLYEEGFLHAKTIVVDGKYVSTGTANFDIRSFKL
CCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECEEEECCCCCCEEEEEEE
NFEIAAFIYDHRIGKTFQKLFLADMERAMPYTQKVIKAKTRPKQLSEELSRLLAPIL
EEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWIWLVLGFLYIINALTAIITVFRSHRDISAVWAWLLVLLLLPVVGFLIYFVFGRKLRAN
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RMQDLMTQRLLGIDDLVDQQQQALAAGQPLIGTPGSGGVSELVRTLLRADDALVTTHNDV
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCE
QVLALREAFFKQLFEDIAVATEHIHIEAYTIRPDAIGHQLRDLLIEKAHAGVTVRVLYDT
EEHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEC
FGSHDLPNNFWKKLTAAGGQVERFVATKLGRWNPRINFRNHRKLIIIDEQLAYLGGFNLG
CCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCC
KEHHGLPMLRDTQLRIQGKAVAVAQARFLMDWNGTSKQQKVKNVDVLFREPVVTGNTTMQ
CCCCCCCCEECCEEEEECCEEEEEHEEEEEECCCCCHHHHHHCHHEEEECCEECCCCEEE
IVSGGPEQQVEAIKLGYLGLINQAKRSIWIESPYFVPDDSLLDALTIAANAGVDVRVMIP
EECCCCHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEC
KKANQILMAKASLFYLNQVVANGGQAFLYEEGFLHAKTIVVDGKYVSTGTANFDIRSFKL
CCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECEEEECCCCCCEEEEEEE
NFEIAAFIYDHRIGKTFQKLFLADMERAMPYTQKVIKAKTRPKQLSEELSRLLAPIL
EEEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]