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The map label for this gene is thiI

Identifier: 116494740

GI number: 116494740

Start: 1226381

End: 1227598

Strand: Direct

Name: thiI

Synonym: LSEI_1248

Alternate gene names: 116494740

Gene position: 1226381-1227598 (Clockwise)

Preceding gene: 116494739

Following gene: 116494741

Centisome position: 42.36

GC content: 47.7

Gene sequence:

>1218_bases
ATGCAATATTCAGAAATTATGGTTCGTTATGGCGAACTTTCGACAAAAGGCAAGAACCGTCAAGCCTTTATCGGCCGGTT
AAACGGCAATGTCACCCGTGCCTTGCACGAATTCCCCAATCTTACGATTCGTCCTAAGCGTGATCGGATGCATATCGAAT
TGAATGGGGAGCCGAGTGATCAGGTGATGGCTCGGTTATCACAAGTTTTTGGCATTCAAAATTTTTCACCAAGTATTGCG
GTTGAAAAAGATATGGATAAGGTACATGCAGTTGCCTTGCAATTAATGAACGAAACCGCTCCAAAGGGCATTAGCTACAA
GGTCAACACGCGCCGCAGTGACCACGATTTTGCCTTAGATACAAACGCCATGAATTTGGATCTGGGTGACTATCTGACAG
ATAAGCGGCCTGATTTGGTTGTTAAAATGCATCAGCCCGACATGATTCTGCGGGTTGAAGTCCGGCGCGAGGCGATTTAT
TTGTCCACGAAGACCATTCAAGGGGCAGGTGGGTTGCCAGTCGGGACTGCCGGCAAAGCAGCTTTGATGCTAAGTGGCGG
CATTGATTCGCCAGTAGCTGGCTATTACGCGCTAAAGCGGGGTGTGGACATTGAGATGGTTCACTTTTTCAGCCCGCCGT
ACACCAGCCAGCAGGCACTCAACAAAGCTAAACAGCTGACCGCAAAGTTGACGCCATATGTCGGCCGGATTTACTTTATC
GAAGTGCCATTTACGGAAATTCAAGAGGAAATCAAGGCGAAAGTGCCAGAAGGTTATCTCATGACCGTTCAACGGCGATT
GATGTTGCGGCTAACGGAAGCCATTGCTCAGCAGCGTGGCGATCTCGCGATTTTCAATGGCGAATCGGTCGGTCAAGTTG
CCTCGCAAACGTTGGAGTCGATGGCGGCAATCAATGATGTGACAACGATGCCGATTATTCGACCAGTGGCAACGATGGAT
AAGAATGAAATCATTGCCGAAGCCGAGAAAATTGACACTTATGATTTGTCGATCATGCCATTTGAAGATTGCTGCACGAT
TTTTGCGCCGCCTTCACCGAAAACTAAGCCAAAGACGGATCGCGCGCGTTACTATGAAAGCAAAATTGATGTCGCCGGCT
TGATGGATCGCGCACTGGCTGGCGTGAAGATTCAGGAGATCAAGTCATCGGATCAATTTATGAATCAAGATCAAGATGTT
ATTGCTGAATTACTTTAA

Upstream 100 bases:

>100_bases
ACTTTGGCCGAGGCAGATGCTTTTAACAAGGCATTTGATACGATTTATACGAAGTTTGCTAAACTTGATAAGGCGACCGT
TTAACTGAAAGGATTATAGT

Downstream 100 bases:

>100_bases
AAAGAATGCCTGTTGTTTGCCTATTGATGATAGTGGTCATGGCAAACAACGGGCGTTTTTATTTAATAAAGGAAACATTT
CACAAAAGCTGAGACTTTTG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MQYSEIMVRYGELSTKGKNRQAFIGRLNGNVTRALHEFPNLTIRPKRDRMHIELNGEPSDQVMARLSQVFGIQNFSPSIA
VEKDMDKVHAVALQLMNETAPKGISYKVNTRRSDHDFALDTNAMNLDLGDYLTDKRPDLVVKMHQPDMILRVEVRREAIY
LSTKTIQGAGGLPVGTAGKAALMLSGGIDSPVAGYYALKRGVDIEMVHFFSPPYTSQQALNKAKQLTAKLTPYVGRIYFI
EVPFTEIQEEIKAKVPEGYLMTVQRRLMLRLTEAIAQQRGDLAIFNGESVGQVASQTLESMAAINDVTTMPIIRPVATMD
KNEIIAEAEKIDTYDLSIMPFEDCCTIFAPPSPKTKPKTDRARYYESKIDVAGLMDRALAGVKIQEIKSSDQFMNQDQDV
IAELL

Sequences:

>Translated_405_residues
MQYSEIMVRYGELSTKGKNRQAFIGRLNGNVTRALHEFPNLTIRPKRDRMHIELNGEPSDQVMARLSQVFGIQNFSPSIA
VEKDMDKVHAVALQLMNETAPKGISYKVNTRRSDHDFALDTNAMNLDLGDYLTDKRPDLVVKMHQPDMILRVEVRREAIY
LSTKTIQGAGGLPVGTAGKAALMLSGGIDSPVAGYYALKRGVDIEMVHFFSPPYTSQQALNKAKQLTAKLTPYVGRIYFI
EVPFTEIQEEIKAKVPEGYLMTVQRRLMLRLTEAIAQQRGDLAIFNGESVGQVASQTLESMAAINDVTTMPIIRPVATMD
KNEIIAEAEKIDTYDLSIMPFEDCCTIFAPPSPKTKPKTDRARYYESKIDVAGLMDRALAGVKIQEIKSSDQFMNQDQDV
IAELL
>Mature_405_residues
MQYSEIMVRYGELSTKGKNRQAFIGRLNGNVTRALHEFPNLTIRPKRDRMHIELNGEPSDQVMARLSQVFGIQNFSPSIA
VEKDMDKVHAVALQLMNETAPKGISYKVNTRRSDHDFALDTNAMNLDLGDYLTDKRPDLVVKMHQPDMILRVEVRREAIY
LSTKTIQGAGGLPVGTAGKAALMLSGGIDSPVAGYYALKRGVDIEMVHFFSPPYTSQQALNKAKQLTAKLTPYVGRIYFI
EVPFTEIQEEIKAKVPEGYLMTVQRRLMLRLTEAIAQQRGDLAIFNGESVGQVASQTLESMAAINDVTTMPIIRPVATMD
KNEIIAEAEKIDTYDLSIMPFEDCCTIFAPPSPKTKPKTDRARYYESKIDVAGLMDRALAGVKIQEIKSSDQFMNQDQDV
IAELL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=399, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_LACC3 (Q039U0)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806474.1
- ProteinModelPortal:   Q039U0
- SMR:   Q039U0
- STRING:   Q039U0
- GeneID:   4419544
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1248
- eggNOG:   COG0301
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   LCAS321967:LSEI_1248-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 45271; Mature: 45271

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS51165 THUMP

Important sites: BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYSEIMVRYGELSTKGKNRQAFIGRLNGNVTRALHEFPNLTIRPKRDRMHIELNGEPSD
CCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCCEEEEEECCCCHH
QVMARLSQVFGIQNFSPSIAVEKDMDKVHAVALQLMNETAPKGISYKVNTRRSDHDFALD
HHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEE
TNAMNLDLGDYLTDKRPDLVVKMHQPDMILRVEVRREAIYLSTKTIQGAGGLPVGTAGKA
CCCEEECHHHHHCCCCCCEEEEECCCCEEEEEEEEEEEEEEEEEEECCCCCCCCCCCCCE
ALMLSGGIDSPVAGYYALKRGVDIEMVHFFSPPYTSQQALNKAKQLTAKLTPYVGRIYFI
EEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
EVPFTEIQEEIKAKVPEGYLMTVQRRLMLRLTEAIAQQRGDLAIFNGESVGQVASQTLES
ECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH
MAAINDVTTMPIIRPVATMDKNEIIAEAEKIDTYDLSIMPFEDCCTIFAPPSPKTKPKTD
HHHHHCCHHCHHHHHHHHCCCHHHHHHHHHCCEEEEEEEEHHHHHEEECCCCCCCCCCCH
RARYYESKIDVAGLMDRALAGVKIQEIKSSDQFMNQDQDVIAELL
HHHHHHHHCCHHHHHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHC
>Mature Secondary Structure
MQYSEIMVRYGELSTKGKNRQAFIGRLNGNVTRALHEFPNLTIRPKRDRMHIELNGEPSD
CCHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCCEEEEEECCCCHH
QVMARLSQVFGIQNFSPSIAVEKDMDKVHAVALQLMNETAPKGISYKVNTRRSDHDFALD
HHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEE
TNAMNLDLGDYLTDKRPDLVVKMHQPDMILRVEVRREAIYLSTKTIQGAGGLPVGTAGKA
CCCEEECHHHHHCCCCCCEEEEECCCCEEEEEEEEEEEEEEEEEEECCCCCCCCCCCCCE
ALMLSGGIDSPVAGYYALKRGVDIEMVHFFSPPYTSQQALNKAKQLTAKLTPYVGRIYFI
EEEEECCCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
EVPFTEIQEEIKAKVPEGYLMTVQRRLMLRLTEAIAQQRGDLAIFNGESVGQVASQTLES
ECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHH
MAAINDVTTMPIIRPVATMDKNEIIAEAEKIDTYDLSIMPFEDCCTIFAPPSPKTKPKTD
HHHHHCCHHCHHHHHHHHCCCHHHHHHHHHCCEEEEEEEEHHHHHEEECCCCCCCCCCCH
RARYYESKIDVAGLMDRALAGVKIQEIKSSDQFMNQDQDVIAELL
HHHHHHHHCCHHHHHHHHHCCCCHHHHCCHHHHHCCCHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA