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The map label for this gene is nisX1 [H]

Identifier: 116494735

GI number: 116494735

Start: 1219947

End: 1220762

Strand: Direct

Name: nisX1 [H]

Synonym: LSEI_1242

Alternate gene names: 116494735

Gene position: 1219947-1220762 (Clockwise)

Preceding gene: 116494734

Following gene: 116494739

Centisome position: 42.14

GC content: 44.12

Gene sequence:

>816_bases
TTGTTAGACGTATCAACGAGTGGTTATTATGCTTGGCGGAGAGCTTTGCCAAAGCGAAACGCCAAAGAAAGGCAATATCG
TCAACGAATCAAACACTTTTTTGATCAAGGACAGGGCACTTACGGGCCCAAACGCATCTGCGGTTTATTGCGTAAAAATG
GTAATAAAGCTTCCTATCACCGAGTTGCCCGTCTGATGGCTGATGCCGGTCTGTATTCAACGCTACGTTGTCGTCATCCG
AAGAGTTTGACCGATAGCCGCCTTGCTCGTCAAGGTGATTATCCCAATCTAGTGAAACACCAATCCTTTAAGCCGTTTGA
AGCCTTATCAAGTGACATTACACAGACGACAACATCAGAAGGAAAAGCTTACATTTGCCAAATAAGGGATTTGACCAGCA
ACCTAGTGTTAGCTCATCAAATATCCAATCATATGGACATGGATTTAGTCTTAGCGACACTTAAACAAGCCCACCAAAGG
TGGGCTTTGCCAGAAACGTGTATTTTTCATAGTGACCGTGGTAGCCAATACACGGCCAAAGCAGTGACAAAGTTGGTCAA
CCAATATCATTGGCAACGTAGCTTCTCAGCATTGGGAAAACCGGGTGATAACGCTTGGAGCGAAAGCTTCTTTGCCATCA
TGAAGAAAGAGCTCATTTATCAGATACAATTTAAAGATATCAATGATCTGAGAGCGCAAGTCTTCGCTTACATCGAGACC
TTTTACAACCGCGTAAGGGTACAAGCACGCTTAGACTATCTTGCACCAGTAGCATGGCTTTCCTTGTATGACCAAGCTTC
ACAAAAAGTCGCTTAG

Upstream 100 bases:

>100_bases
AAGCCGCCGCCTACTTTGCCAGTCTCCAGAAGTAACCCCCAGCGATAAGTATGCTTTTATGTTGAGGGAAACCTCTGTTT
CTGTGGTAAGGTGGGCGCGT

Downstream 100 bases:

>100_bases
CAAACTGTCCGAAAAAGTGTTGACTTCCCAAGCTTAAAATTTTGCAAACTTGGAGATCAAAAAAACTAGGCGGACGTTAT
ACGCAGTCAAACTGGTTTTT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYHRVARLMADAGLYSTLRCRHP
KSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLTSNLVLAHQISNHMDMDLVLATLKQAHQR
WALPETCIFHSDRGSQYTAKAVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINDLRAQVFAYIET
FYNRVRVQARLDYLAPVAWLSLYDQASQKVA

Sequences:

>Translated_271_residues
MLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYHRVARLMADAGLYSTLRCRHP
KSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLTSNLVLAHQISNHMDMDLVLATLKQAHQR
WALPETCIFHSDRGSQYTAKAVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINDLRAQVFAYIET
FYNRVRVQARLDYLAPVAWLSLYDQASQKVA
>Mature_271_residues
MLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYHRVARLMADAGLYSTLRCRHP
KSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSEGKAYICQIRDLTSNLVLAHQISNHMDMDLVLATLKQAHQR
WALPETCIFHSDRGSQYTAKAVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINDLRAQVFAYIET
FYNRVRVQARLDYLAPVAWLSLYDQASQKVA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=204, Percent_Identity=26.9607843137255, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789981, Length=259, Percent_Identity=27.4131274131274, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1788405, Length=266, Percent_Identity=30.4511278195489, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787263, Length=266, Percent_Identity=30.4511278195489, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786753, Length=266, Percent_Identity=30.4511278195489, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786570, Length=266, Percent_Identity=30.4511278195489, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786490, Length=266, Percent_Identity=30.4511278195489, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31352; Mature: 31352

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYH
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
RVARLMADAGLYSTLRCRHPKSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSE
HHHHHHHHHCHHHHHHCCCCCCCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCC
GKAYICQIRDLTSNLVLAHQISNHMDMDLVLATLKQAHQRWALPETCIFHSDRGSQYTAK
CCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCHHHH
AVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINDLRAQVFAYIET
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHH
FYNRVRVQARLDYLAPVAWLSLYDQASQKVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDVSTSGYYAWRRALPKRNAKERQYRQRIKHFFDQGQGTYGPKRICGLLRKNGNKASYH
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHH
RVARLMADAGLYSTLRCRHPKSLTDSRLARQGDYPNLVKHQSFKPFEALSSDITQTTTSE
HHHHHHHHHCHHHHHHCCCCCCCHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHCCC
GKAYICQIRDLTSNLVLAHQISNHMDMDLVLATLKQAHQRWALPETCIFHSDRGSQYTAK
CCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCHHHH
AVTKLVNQYHWQRSFSALGKPGDNAWSESFFAIMKKELIYQIQFKDINDLRAQVFAYIET
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHH
FYNRVRVQARLDYLAPVAWLSLYDQASQKVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1482189; 11337471; 1482192 [H]