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The map label for this gene is potB [H]

Identifier: 116494511

GI number: 116494511

Start: 992223

End: 993047

Strand: Direct

Name: potB [H]

Synonym: LSEI_1007

Alternate gene names: 116494511

Gene position: 992223-993047 (Clockwise)

Preceding gene: 116494510

Following gene: 116494512

Centisome position: 34.27

GC content: 44.61

Gene sequence:

>825_bases
GTGAAAAAATCCACCACAAACGCCGCATTCTACACACCTTATGTGATGTGGCTGGCATTATTTGTGATCGCACCGATGGT
TTTAATCGTTTATCAAAGTTTCTTTAACATCAGCGGTCACTTTACCCTTGCCAATTATCAGACCTATTTTCAATCAGGCA
CTTATATTATGATGACGATCAATTCCGTCTGGTATGCGTTCCTGATTACGTTAGCAACCTTATTAATCAGCTATCCAACA
GCATATTTGCTGCATTATGCCAAGCATAAACAGTTATGGTTGTTGCTGATTATTCTACCGACTTGGATTAACTTGCTGCT
GAAAGCATACGCCTTCATCGGGATCTTCAGTCAGGATGGCGGGGTTAATAGTTTCTTGGGAATGTTTGGCATCGCGCCCC
AGCAATTCCTGTTCACGGATTTTAGTTTCATCTTTGTTGCTGCTTATATTGAAATCCCATTCATGATCTTGCCGATTTTC
AATGCAATCGAAGAATTACCGGAAAACCTTGTCAATGCTGCCCAAGATTTAGGGGCCAAAAGCTGGCAGACCTTTACCAA
GGTGATCTGGCCGTTAACAATTTCCGGCGTGAAATCCGGGGTTCAAGCTGTTTTCATTCCTAGTTTAAGTTTATTCATGT
TGACGCGTCTGATCGGCGGGAACCGGGTCATTACTTTGGGGACAGCCATTGAAGAGCATTTTCTGACAACGATGAACTGG
GGCATGGGTTCGACGATCGGGGTTGTCCTGATCGTCGCCATGTTCATCATCATGTTCCTGACCGGTGAACGTAAGAAGAA
GGGGGCGCGGCGTCATGAAGCGTAA

Upstream 100 bases:

>100_bases
ACGTCAGTAAGGATCAGTTGGGGCAGTGTCATCGAAGCGTTTTGACGATTAGATAGACACTGGTCGGCGCTGATCGATTT
TCGAACTGAGGAGGGTCATC

Downstream 100 bases:

>100_bases
ATTCAAATGGTCCAACCTTTATCTGATCTTCGTCTTCATCTGTTTGTATGTGCCGATCTTTTATTTGGTTATCTACTCGT
TTTCAACGGGAGATCGTATG

Product: spermidine/putrescine ABC transporter permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKKSTTNAAFYTPYVMWLALFVIAPMVLIVYQSFFNISGHFTLANYQTYFQSGTYIMMTINSVWYAFLITLATLLISYPT
AYLLHYAKHKQLWLLLIILPTWINLLLKAYAFIGIFSQDGGVNSFLGMFGIAPQQFLFTDFSFIFVAAYIEIPFMILPIF
NAIEELPENLVNAAQDLGAKSWQTFTKVIWPLTISGVKSGVQAVFIPSLSLFMLTRLIGGNRVITLGTAIEEHFLTTMNW
GMGSTIGVVLIVAMFIIMFLTGERKKKGARRHEA

Sequences:

>Translated_274_residues
MKKSTTNAAFYTPYVMWLALFVIAPMVLIVYQSFFNISGHFTLANYQTYFQSGTYIMMTINSVWYAFLITLATLLISYPT
AYLLHYAKHKQLWLLLIILPTWINLLLKAYAFIGIFSQDGGVNSFLGMFGIAPQQFLFTDFSFIFVAAYIEIPFMILPIF
NAIEELPENLVNAAQDLGAKSWQTFTKVIWPLTISGVKSGVQAVFIPSLSLFMLTRLIGGNRVITLGTAIEEHFLTTMNW
GMGSTIGVVLIVAMFIIMFLTGERKKKGARRHEA
>Mature_274_residues
MKKSTTNAAFYTPYVMWLALFVIAPMVLIVYQSFFNISGHFTLANYQTYFQSGTYIMMTINSVWYAFLITLATLLISYPT
AYLLHYAKHKQLWLLLIILPTWINLLLKAYAFIGIFSQDGGVNSFLGMFGIAPQQFLFTDFSFIFVAAYIEIPFMILPIF
NAIEELPENLVNAAQDLGAKSWQTFTKVIWPLTISGVKSGVQAVFIPSLSLFMLTRLIGGNRVITLGTAIEEHFLTTMNW
GMGSTIGVVLIVAMFIIMFLTGERKKKGARRHEA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=248, Percent_Identity=35.0806451612903, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1787080, Length=236, Percent_Identity=30.9322033898305, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787713, Length=244, Percent_Identity=29.0983606557377, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30943; Mature: 30943

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00589 PTS_HPR_SER ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]