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The map label for this gene is ppnK

Identifier: 116494410

GI number: 116494410

Start: 878421

End: 879218

Strand: Direct

Name: ppnK

Synonym: LSEI_0902

Alternate gene names: 116494410

Gene position: 878421-879218 (Clockwise)

Preceding gene: 116494409

Following gene: 116494411

Centisome position: 30.34

GC content: 47.49

Gene sequence:

>798_bases
ATGCGTGTGACAGTTTTCCATAATAGCATACCTGCCTCAATCACGGCTGCGCAAAAGCTGACGAAGCTTTTGAAGAGCGG
TCACTTTGAGCTTGATGAGCGGCATCCCGAGGTGGTCGTTACCATCGGCGGTGATGGTACGCTCCTTTCGGCCTTTCATC
GCTATGCGGATCAGTTAAACTCAATTCGCTTCATCGGTGTGCATACAGGCCATTTGGGTTTTTACACAGACTGGCGGGAT
TTTGAAATTGAAGATTTAGTTATCGCCTTAAAAGAAGACTCTGGACAAAGCGTTAGCTACCCACTTTTGGATGTTCAGGC
GACTTATGCTGACGCGACCAGTGCTCATTATCTAGCATTGAATGAGTCAACCTTGAAGCGTTTAAACGGTACTATGCGGA
CGGAAGTCTATATCAAAGGCGATTTCTTTGAGAGCTTCCGCGGGGATGGTTTGTGTGTGTCGACGCCAACTGGATCTACA
GCTTACAGTAAGTCTAACGGCGGTGCTGTGATTCATCCGCGATTGGATGCGTTGCAAATGACCGAAATTGCGTCCATTAA
TAATCGGGTTTTTCGTACCCTCAGTTCGCCGATTATCACGGCACCAGACGAGTGGGTGACATTGGAGCCGACAGGTCGCG
ATGATTACGTCATGACTGTGGATCAGTTCGTCATCAATCCACCAACAATCAAACAAATTCGGTATAAAATTGCGAAAGAA
CGGATTCATTTTGCACGCTACCGGCATATGCATTTCTGGGATCGTGTTGAAGACGCCTTTATCGGAGCCAAACACTGA

Upstream 100 bases:

>100_bases
GAAGAAATTCAAGAAGCCCAGCATCTGTTTGTTTATGGTAAAGGGCAGCAGCCACATTCGAAAACAAAGGATCACCATGA
CGATAAAAAGGATGAGTAAG

Downstream 100 bases:

>100_bases
TGCGATTTGAATGGCAGTATGAGGGACCGCCGCTGAAATTGGCGGCTTTTTTAAAACAACAGGGTTTTTCCCGTGCACAG
CTTAAGAAACTTCGTTATCA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRVTVFHNSIPASITAAQKLTKLLKSGHFELDERHPEVVVTIGGDGTLLSAFHRYADQLNSIRFIGVHTGHLGFYTDWRD
FEIEDLVIALKEDSGQSVSYPLLDVQATYADATSAHYLALNESTLKRLNGTMRTEVYIKGDFFESFRGDGLCVSTPTGST
AYSKSNGGAVIHPRLDALQMTEIASINNRVFRTLSSPIITAPDEWVTLEPTGRDDYVMTVDQFVINPPTIKQIRYKIAKE
RIHFARYRHMHFWDRVEDAFIGAKH

Sequences:

>Translated_265_residues
MRVTVFHNSIPASITAAQKLTKLLKSGHFELDERHPEVVVTIGGDGTLLSAFHRYADQLNSIRFIGVHTGHLGFYTDWRD
FEIEDLVIALKEDSGQSVSYPLLDVQATYADATSAHYLALNESTLKRLNGTMRTEVYIKGDFFESFRGDGLCVSTPTGST
AYSKSNGGAVIHPRLDALQMTEIASINNRVFRTLSSPIITAPDEWVTLEPTGRDDYVMTVDQFVINPPTIKQIRYKIAKE
RIHFARYRHMHFWDRVEDAFIGAKH
>Mature_265_residues
MRVTVFHNSIPASITAAQKLTKLLKSGHFELDERHPEVVVTIGGDGTLLSAFHRYADQLNSIRFIGVHTGHLGFYTDWRD
FEIEDLVIALKEDSGQSVSYPLLDVQATYADATSAHYLALNESTLKRLNGTMRTEVYIKGDFFESFRGDGLCVSTPTGST
AYSKSNGGAVIHPRLDALQMTEIASINNRVFRTLSSPIITAPDEWVTLEPTGRDDYVMTVDQFVINPPTIKQIRYKIAKE
RIHFARYRHMHFWDRVEDAFIGAKH

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=164, Percent_Identity=34.7560975609756, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320794, Length=188, Percent_Identity=31.9148936170213, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=152, Percent_Identity=29.6052631578947, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_LACC3 (Q03AS0)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806144.1
- ProteinModelPortal:   Q03AS0
- SMR:   Q03AS0
- STRING:   Q03AS0
- GeneID:   4421717
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0902
- eggNOG:   COG0061
- HOGENOM:   HBG314020
- OMA:   PSIRAIQ
- ProtClustDB:   PRK04885
- BioCyc:   LCAS321967:LSEI_0902-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 29893; Mature: 29893

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTVFHNSIPASITAAQKLTKLLKSGHFELDERHPEVVVTIGGDGTLLSAFHRYADQLN
CEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHC
SIRFIGVHTGHLGFYTDWRDFEIEDLVIALKEDSGQSVSYPLLDVQATYADATSAHYLAL
CEEEEEEEECCEEEEECCCCCCCEEEEEEEECCCCCEEECCEEEEEEECCCCCCEEEEEE
NESTLKRLNGTMRTEVYIKGDFFESFRGDGLCVSTPTGSTAYSKSNGGAVIHPRLDALQM
CHHHHHHHCCCEEEEEEEEECHHHHHCCCCEEEECCCCCCCEECCCCCEEEECCCCHHHH
TEIASINNRVFRTLSSPIITAPDEWVTLEPTGRDDYVMTVDQFVINPPTIKQIRYKIAKE
HHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCEEEEEEHEEECCCHHHHHHHHHHHH
RIHFARYRHMHFWDRVEDAFIGAKH
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRVTVFHNSIPASITAAQKLTKLLKSGHFELDERHPEVVVTIGGDGTLLSAFHRYADQLN
CEEEEEECCCCCHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHHC
SIRFIGVHTGHLGFYTDWRDFEIEDLVIALKEDSGQSVSYPLLDVQATYADATSAHYLAL
CEEEEEEEECCEEEEECCCCCCCEEEEEEEECCCCCEEECCEEEEEEECCCCCCEEEEEE
NESTLKRLNGTMRTEVYIKGDFFESFRGDGLCVSTPTGSTAYSKSNGGAVIHPRLDALQM
CHHHHHHHCCCEEEEEEEEECHHHHHCCCCEEEECCCCCCCEECCCCCEEEECCCCHHHH
TEIASINNRVFRTLSSPIITAPDEWVTLEPTGRDDYVMTVDQFVINPPTIKQIRYKIAKE
HHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCCCEEEEEEHEEECCCHHHHHHHHHHHH
RIHFARYRHMHFWDRVEDAFIGAKH
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA