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The map label for this gene is patB [H]

Identifier: 116494402

GI number: 116494402

Start: 870385

End: 871590

Strand: Direct

Name: patB [H]

Synonym: LSEI_0894

Alternate gene names: 116494402

Gene position: 870385-871590 (Clockwise)

Preceding gene: 116494401

Following gene: 116494404

Centisome position: 30.06

GC content: 48.92

Gene sequence:

>1206_bases
ATGGAAGAATTGCCAGCTGATGTAGCGGCTTTTGTTGAAACACATTTGGTTGATCGGCGCAATAGTAACGCCGTGAAGTG
GGATGGTTTGGCAGGTGAATTTGGCCGCACGGATCTCCTGCCGATGTGGATTGCCGATACCGAATTTAAAGCACCTCAGG
CGGTGATTGACGCCTTAACCGCTCGCATTCAAGAAGGAACTTTTGGCTATTCCATTCGGCCACAATCCTACTATGATGCC
TTCATTGATTGGGAAAAGACTCGCCATGGCGTGACGGTGCAGCCGGAATGGATGCGTTTTGGTGTCGGTGTGGTTAAATC
GCTGTATGCCATGGTTAATTGGCTGACCGAACCCGGCGATCCAGTTTTAATCATGCAGCCTGTCTATTATCCCTTCATGA
ATGCGATTAAGGATCTTGGTCGCAAAGTGGTCTCGGTTGATCTTCAATTAACTGCTGATGGCTGGCGGATAGATTTTGAT
CAATTGGAGAAAACGCTGGCGACGCCAAACATTAAAGCCATGATTTTTTGCTCGCCACATAATCCGGTCGGTCGAGTTTG
GACCCGTGAAGAACTGGAACGGCTTTTTGCGATCACGAGTCACCATGATGTCACGGTGGTATCTGATGAGATTCACGGCG
ATTTGGAAGTTAGTGGGCCAAAATTCACCTCTGCTTTGCAAGTAGCTGAGGGTCCGGCACGCAGTCGGCTTGTCGTCCTG
AATGCACCGTCGAAGACCTTTAATCTTGCGGCTTTACTGAATTCGCATATTATTATTCCGGATGAAAAGTTGCGCACAGC
TTATGATGATTTCATCAAACAGTTGCATCCAGTAGACACCAGCCTTTTGGGACAGGTCGCCGGTGAGGCAGCTTATCGTC
ACGGTGGCGCGTGGTTAGACAAGGTGCTTCAAGTTGTCCGATATAACTACCAACAGTTGCTAACCGGTCTGAAGCAGAGT
GCTCCGAAGGCTACAGTTGCGGACTTGCAGGGCACCTATCTTGCCTATGTGGATATTGGTGCCTATGTTGCGCCAGCCAA
AATTAAATCATTTGTTGAAGATGTTTGCGGACTGGCAGTTGATTACGGCGCATGGTTCTCCCCAAAAACAGCTACGTATA
TTCGCTTGAACTTAGCCACCGACCCCAAACTTGTTGCCGAGGCGATTCAGCGTTTAACTGAGCACTTGGCTCAGCCGCAA
CTTTAA

Upstream 100 bases:

>100_bases
CCACTTATGCTCCGGTTTCTAACCGGGCCTGCTCACGCTCTGGACACTCATGCATGTGATGCGTACAATGACATCAAAAG
TAAGAAAGCAGGGATGATTG

Downstream 100 bases:

>100_bases
TTGAAACAGAAAAAAACACCCTGTTAACGCAAGGCATGTTGACTCATTACCAAACATACAAGGTTTGGTGCTGAGACAGC
GACGCTTGCTGATGGCAGGG

Product: bifunctional beta-cystathionase/maltose regulon repressor

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MEELPADVAAFVETHLVDRRNSNAVKWDGLAGEFGRTDLLPMWIADTEFKAPQAVIDALTARIQEGTFGYSIRPQSYYDA
FIDWEKTRHGVTVQPEWMRFGVGVVKSLYAMVNWLTEPGDPVLIMQPVYYPFMNAIKDLGRKVVSVDLQLTADGWRIDFD
QLEKTLATPNIKAMIFCSPHNPVGRVWTREELERLFAITSHHDVTVVSDEIHGDLEVSGPKFTSALQVAEGPARSRLVVL
NAPSKTFNLAALLNSHIIIPDEKLRTAYDDFIKQLHPVDTSLLGQVAGEAAYRHGGAWLDKVLQVVRYNYQQLLTGLKQS
APKATVADLQGTYLAYVDIGAYVAPAKIKSFVEDVCGLAVDYGAWFSPKTATYIRLNLATDPKLVAEAIQRLTEHLAQPQ
L

Sequences:

>Translated_401_residues
MEELPADVAAFVETHLVDRRNSNAVKWDGLAGEFGRTDLLPMWIADTEFKAPQAVIDALTARIQEGTFGYSIRPQSYYDA
FIDWEKTRHGVTVQPEWMRFGVGVVKSLYAMVNWLTEPGDPVLIMQPVYYPFMNAIKDLGRKVVSVDLQLTADGWRIDFD
QLEKTLATPNIKAMIFCSPHNPVGRVWTREELERLFAITSHHDVTVVSDEIHGDLEVSGPKFTSALQVAEGPARSRLVVL
NAPSKTFNLAALLNSHIIIPDEKLRTAYDDFIKQLHPVDTSLLGQVAGEAAYRHGGAWLDKVLQVVRYNYQQLLTGLKQS
APKATVADLQGTYLAYVDIGAYVAPAKIKSFVEDVCGLAVDYGAWFSPKTATYIRLNLATDPKLVAEAIQRLTEHLAQPQ
L
>Mature_401_residues
MEELPADVAAFVETHLVDRRNSNAVKWDGLAGEFGRTDLLPMWIADTEFKAPQAVIDALTARIQEGTFGYSIRPQSYYDA
FIDWEKTRHGVTVQPEWMRFGVGVVKSLYAMVNWLTEPGDPVLIMQPVYYPFMNAIKDLGRKVVSVDLQLTADGWRIDFD
QLEKTLATPNIKAMIFCSPHNPVGRVWTREELERLFAITSHHDVTVVSDEIHGDLEVSGPKFTSALQVAEGPARSRLVVL
NAPSKTFNLAALLNSHIIIPDEKLRTAYDDFIKQLHPVDTSLLGQVAGEAAYRHGGAWLDKVLQVVRYNYQQLLTGLKQS
APKATVADLQGTYLAYVDIGAYVAPAKIKSFVEDVCGLAVDYGAWFSPKTATYIRLNLATDPKLVAEAIQRLTEHLAQPQ
L

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713254, Length=159, Percent_Identity=28.3018867924528, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI56713256, Length=159, Percent_Identity=28.3018867924528, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1787909, Length=331, Percent_Identity=33.5347432024169, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1786816, Length=218, Percent_Identity=24.3119266055046, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787710, Length=128, Percent_Identity=27.34375, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17567369, Length=126, Percent_Identity=32.5396825396825, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994472, Length=117, Percent_Identity=34.1880341880342, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71994476, Length=117, Percent_Identity=34.1880341880342, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322401, Length=122, Percent_Identity=35.2459016393443, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 44699; Mature: 44699

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEELPADVAAFVETHLVDRRNSNAVKWDGLAGEFGRTDLLPMWIADTEFKAPQAVIDALT
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
ARIQEGTFGYSIRPQSYYDAFIDWEKTRHGVTVQPEWMRFGVGVVKSLYAMVNWLTEPGD
HHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PVLIMQPVYYPFMNAIKDLGRKVVSVDLQLTADGWRIDFDQLEKTLATPNIKAMIFCSPH
CEEEECCHHHHHHHHHHHHCHHEEEEEEEEECCCCEECHHHHHHHHCCCCCEEEEEECCC
NPVGRVWTREELERLFAITSHHDVTVVSDEIHGDLEVSGPKFTSALQVAEGPARSRLVVL
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCHHHHHHHHCCCCCCEEEEE
NAPSKTFNLAALLNSHIIIPDEKLRTAYDDFIKQLHPVDTSLLGQVAGEAAYRHGGAWLD
ECCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
KVLQVVRYNYQQLLTGLKQSAPKATVADLQGTYLAYVDIGAYVAPAKIKSFVEDVCGLAV
HHHHHHHHCHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCEECHHHHHHHHHHHHHHEE
DYGAWFSPKTATYIRLNLATDPKLVAEAIQRLTEHLAQPQL
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEELPADVAAFVETHLVDRRNSNAVKWDGLAGEFGRTDLLPMWIADTEFKAPQAVIDALT
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
ARIQEGTFGYSIRPQSYYDAFIDWEKTRHGVTVQPEWMRFGVGVVKSLYAMVNWLTEPGD
HHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PVLIMQPVYYPFMNAIKDLGRKVVSVDLQLTADGWRIDFDQLEKTLATPNIKAMIFCSPH
CEEEECCHHHHHHHHHHHHCHHEEEEEEEEECCCCEECHHHHHHHHCCCCCEEEEEECCC
NPVGRVWTREELERLFAITSHHDVTVVSDEIHGDLEVSGPKFTSALQVAEGPARSRLVVL
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCHHHHHHHHCCCCCCEEEEE
NAPSKTFNLAALLNSHIIIPDEKLRTAYDDFIKQLHPVDTSLLGQVAGEAAYRHGGAWLD
ECCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHH
KVLQVVRYNYQQLLTGLKQSAPKATVADLQGTYLAYVDIGAYVAPAKIKSFVEDVCGLAV
HHHHHHHHCHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCCEECHHHHHHHHHHHHHHEE
DYGAWFSPKTATYIRLNLATDPKLVAEAIQRLTEHLAQPQL
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]