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The map label for this gene is ycnB [H]

Identifier: 116494389

GI number: 116494389

Start: 855638

End: 857116

Strand: Direct

Name: ycnB [H]

Synonym: LSEI_0880

Alternate gene names: 116494389

Gene position: 855638-857116 (Clockwise)

Preceding gene: 116494387

Following gene: 116494390

Centisome position: 29.55

GC content: 46.45

Gene sequence:

>1479_bases
ATGGACGTTAAAGATATCAACGGGAAACACGTCAATGTACCAATGATGGTCACGACCTTGATCGTTGGGACGTTTATTAC
GATTTTGAATCAGACCATTCTATCTACTGCGTTTCCAACGCTGATGAAGGCCTTCAACATTTCAACTGCGACAGTACAGT
GGTTGTCGACAGGCTTCATGCTCGTAAACGGGATTATGATTCCAGTATCCGCATATCTGAGTGCGAAGGTGCCATCAAAA
TGGTTATACATGAGTGCAATGAGTACTTTTTTTGTCGGCACATTGGTTGCCTTCATTGCAAATAGTTTTGGCGTTTTGCT
AATCGGTCGATTGATTCAGGCGCTTGGCGTTGGGATCACGATGCCATTGCTGCAGAACATCATGATGTCGATTTTTCCCC
CGAACCGGCGCGGCGCTGCTATGGGGATGGCTGGAATTGCGATTGGGGTGGCACCTGCCATCGGGCCAACGCTTTCCGGT
TATGTCATCGACAATTTCGGCTGGCGTTTCTTATTCGGTATGATTCTGCCAATTGCCGGTCTCGTTATTTTGGCAACATT
GTTTTTCTTCAAAAATGTCTTACCGTTGTCTGATCCTAAGTTGGATATTCCTTCCTTGATTGAATCGTCAATCGGGTTCG
GCTCCTTGTTGTATGGCTTTTCTGAAGTCGGTAATGATGGCTGGGGCGATCCAATCGTCTTAGGAACCATCGCGCTTGGT
GTCGCGTTTATCTTCATCTTTGGTTATCGTCAATTACACTTGAAGGAACCGTTTGTCGAGTTGCGGGTGTTTAAGAGCAA
GATTTTTGCTTTATCCACAGTTTTAGGAACGATTGCGAACATGGCGATGGTCGGGGTGGAAATGATTTTGCCAATGTATT
TGCAAATCATTCACGGCAAGAGTGCTTTGGAGTCAGGTTTGACATTGTTGCCTGGGGCGATTTTGATTGCTTTAATGAGC
CCGGTCACTGGTCAAGTGTTTGATCGCATCGGCGCCAAGCGGCTTGCACAGTTAGGCTTGTTCTTGCTGACGATTGCAAC
ATTGCCATACTTTTTCCTGACAGAAAGCACGCCTTCCATTTTTATTACGGTGATTTATGCTGTGCGGATGTTTGGGATTT
CAATGGTCATGATGCCTTTGACGACGAATGGGATGAACGCCTTATCACCTGATATGATTCGACATGGGACAGCGGTTAAC
AACACTGTCCGTCAGGTGGCAACTTCCATGACAACTGCTGTCATGATTTCAGTTCTGTCAAACGTCACCAATCTGGCCAA
ACCGGCGGCCAGTTTGTTGAAAACCAATCCGCTGCAGTATAAGCAAGACTTTTTTGGGGCAACACTTAGTGGCTACCACG
CAGCCTTTCTGCTGGCAGCAGGGTTCAGTTTAATTGGCTGGATCTTGGCTTTCTTCTTGAATACCCACGCGATGTCGCAA
GAAGTGACGATCAAAAGTGAAAAGAAGGTGAAGGCATGA

Upstream 100 bases:

>100_bases
CCTTGACATTTATTAAGTTTAAATGTAATACTTTCTATTGTCAATATTACATTGTGTGATATAGAAAACATGAAATCTTA
TAAAGAAACTAGGCGAAGTT

Downstream 100 bases:

>100_bases
TTCTATGGTTTTTGGGCCTGACAGTGATCGGCCTTTATCTCAGCTTTATTATGATGAAACGCTCCAGTTTGCGTAGCACG
TTAATTGCGGTCTTCGGGAT

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MDVKDINGKHVNVPMMVTTLIVGTFITILNQTILSTAFPTLMKAFNISTATVQWLSTGFMLVNGIMIPVSAYLSAKVPSK
WLYMSAMSTFFVGTLVAFIANSFGVLLIGRLIQALGVGITMPLLQNIMMSIFPPNRRGAAMGMAGIAIGVAPAIGPTLSG
YVIDNFGWRFLFGMILPIAGLVILATLFFFKNVLPLSDPKLDIPSLIESSIGFGSLLYGFSEVGNDGWGDPIVLGTIALG
VAFIFIFGYRQLHLKEPFVELRVFKSKIFALSTVLGTIANMAMVGVEMILPMYLQIIHGKSALESGLTLLPGAILIALMS
PVTGQVFDRIGAKRLAQLGLFLLTIATLPYFFLTESTPSIFITVIYAVRMFGISMVMMPLTTNGMNALSPDMIRHGTAVN
NTVRQVATSMTTAVMISVLSNVTNLAKPAASLLKTNPLQYKQDFFGATLSGYHAAFLLAAGFSLIGWILAFFLNTHAMSQ
EVTIKSEKKVKA

Sequences:

>Translated_492_residues
MDVKDINGKHVNVPMMVTTLIVGTFITILNQTILSTAFPTLMKAFNISTATVQWLSTGFMLVNGIMIPVSAYLSAKVPSK
WLYMSAMSTFFVGTLVAFIANSFGVLLIGRLIQALGVGITMPLLQNIMMSIFPPNRRGAAMGMAGIAIGVAPAIGPTLSG
YVIDNFGWRFLFGMILPIAGLVILATLFFFKNVLPLSDPKLDIPSLIESSIGFGSLLYGFSEVGNDGWGDPIVLGTIALG
VAFIFIFGYRQLHLKEPFVELRVFKSKIFALSTVLGTIANMAMVGVEMILPMYLQIIHGKSALESGLTLLPGAILIALMS
PVTGQVFDRIGAKRLAQLGLFLLTIATLPYFFLTESTPSIFITVIYAVRMFGISMVMMPLTTNGMNALSPDMIRHGTAVN
NTVRQVATSMTTAVMISVLSNVTNLAKPAASLLKTNPLQYKQDFFGATLSGYHAAFLLAAGFSLIGWILAFFLNTHAMSQ
EVTIKSEKKVKA
>Mature_492_residues
MDVKDINGKHVNVPMMVTTLIVGTFITILNQTILSTAFPTLMKAFNISTATVQWLSTGFMLVNGIMIPVSAYLSAKVPSK
WLYMSAMSTFFVGTLVAFIANSFGVLLIGRLIQALGVGITMPLLQNIMMSIFPPNRRGAAMGMAGIAIGVAPAIGPTLSG
YVIDNFGWRFLFGMILPIAGLVILATLFFFKNVLPLSDPKLDIPSLIESSIGFGSLLYGFSEVGNDGWGDPIVLGTIALG
VAFIFIFGYRQLHLKEPFVELRVFKSKIFALSTVLGTIANMAMVGVEMILPMYLQIIHGKSALESGLTLLPGAILIALMS
PVTGQVFDRIGAKRLAQLGLFLLTIATLPYFFLTESTPSIFITVIYAVRMFGISMVMMPLTTNGMNALSPDMIRHGTAVN
NTVRQVATSMTTAVMISVLSNVTNLAKPAASLLKTNPLQYKQDFFGATLSGYHAAFLLAAGFSLIGWILAFFLNTHAMSQ
EVTIKSEKKVKA

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=442, Percent_Identity=26.0180995475113, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1788710, Length=445, Percent_Identity=25.1685393258427, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081983, Length=350, Percent_Identity=25.1428571428571, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=25.4854368932039, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788392, Length=327, Percent_Identity=24.4648318042813, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=323, Percent_Identity=27.2445820433437, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=238, Percent_Identity=23.9495798319328, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53059; Mature: 53059

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CEEECCCCCCCC
>Mature Secondary Structure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CEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]