Click here to switch to the map view.

The map label for this gene is cysK [H]

Identifier: 116494039

GI number: 116494039

Start: 497885

End: 498814

Strand: Direct

Name: cysK [H]

Synonym: LSEI_0480

Alternate gene names: 116494039

Gene position: 497885-498814 (Clockwise)

Preceding gene: 116494038

Following gene: 116494041

Centisome position: 17.2

GC content: 51.61

Gene sequence:

>930_bases
ATGGTGACAGCAGCAGATAATATTACCGGTTTGATTGGCAACACCCCTTTGCTTAAGCTAAATCGTGTCGTCCCAGAAGA
TGCAGCAGATGTTTATGTGAAGCTTGAATTCTTTAATCCCGGCGGTTCTGTCAAAGACCGAATTGCCTTGGCCATGATTG
AAGATGCCGAATACAAGGGCGTTTTGAAGCCAGGCGGCACCATTGTTGAACCAACATCTGGCAACACTGGCATCGGCTTG
GCCTTGGTCGCCGCGGCTAAAGGCTACCACCTGATCATCACGATGCCAGAAACGATGAGTGTTGAACGTCGTGCGTTAAT
GCGGGGCTATGGCGCCGAATTGATTCTGACACCAGGGGCGGATGGCATGCCGGGTGCGATTAAAAAAGCTCAGGCACTCA
GCAAGGAAAATGGTTACTTCTTGCCGATGCAGTTCCAAAATCCGGCTAATCCGGATGTGCACGAGCGCACCACCGGTCAA
GAAATCATCCGTTCTTTTGATGGCGGCACCCCAGACGCCTTCGTTGCTGGCGTGGGCACAGGCGGCACGTTGACCGGGGT
TGGCCGGGCGTTGCGGAAAATCAACCCGCAAGTGCAAATTTATGCACTTGAAGCAGCTGAGTCACCAATGCTCAAAGAAG
GGCACGGCGGCAAGCATAAGATCCAAGGTATCTCCGCTGGCTTCATCCCAGACGTTTTGGATACCAACCTATACCAAGAC
ATTATTGAAGTCACCAGCGACCAAGCCATCGACATGGCTCGTCACGTTAGCCACGAAGAAGGCTTCCTGCCAGGCATCTC
TGCCGGCGCCAATATTTTCGGTGCCATTGAAATTGCTAAGAAACTTGGCAAAGGCAAGAGCGTGGTAACAGTCGCGCCGG
ATAATGGTGAACGATACTTGTCAACGGACTTGTTTAAGTTCGAAGATTAA

Upstream 100 bases:

>100_bases
AACGCAACGAACGATTTTTGACAATTGGATTCAACATGTCGCGGATCATCGCAATGACAATCATTAGGAAAATCAATCAA
CTAATATGGAGGCTTTTAAT

Downstream 100 bases:

>100_bases
AGGTACAATTTTGCAGCTGGCATTGATGAAATAGAACATGAAATGAAAGTGGCTCGGTATCTGACGAAGGTCAGCTATCG
GGCCACTTTTATATGGATAA

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MVTAADNITGLIGNTPLLKLNRVVPEDAADVYVKLEFFNPGGSVKDRIALAMIEDAEYKGVLKPGGTIVEPTSGNTGIGL
ALVAAAKGYHLIITMPETMSVERRALMRGYGAELILTPGADGMPGAIKKAQALSKENGYFLPMQFQNPANPDVHERTTGQ
EIIRSFDGGTPDAFVAGVGTGGTLTGVGRALRKINPQVQIYALEAAESPMLKEGHGGKHKIQGISAGFIPDVLDTNLYQD
IIEVTSDQAIDMARHVSHEEGFLPGISAGANIFGAIEIAKKLGKGKSVVTVAPDNGERYLSTDLFKFED

Sequences:

>Translated_309_residues
MVTAADNITGLIGNTPLLKLNRVVPEDAADVYVKLEFFNPGGSVKDRIALAMIEDAEYKGVLKPGGTIVEPTSGNTGIGL
ALVAAAKGYHLIITMPETMSVERRALMRGYGAELILTPGADGMPGAIKKAQALSKENGYFLPMQFQNPANPDVHERTTGQ
EIIRSFDGGTPDAFVAGVGTGGTLTGVGRALRKINPQVQIYALEAAESPMLKEGHGGKHKIQGISAGFIPDVLDTNLYQD
IIEVTSDQAIDMARHVSHEEGFLPGISAGANIFGAIEIAKKLGKGKSVVTVAPDNGERYLSTDLFKFED
>Mature_309_residues
MVTAADNITGLIGNTPLLKLNRVVPEDAADVYVKLEFFNPGGSVKDRIALAMIEDAEYKGVLKPGGTIVEPTSGNTGIGL
ALVAAAKGYHLIITMPETMSVERRALMRGYGAELILTPGADGMPGAIKKAQALSKENGYFLPMQFQNPANPDVHERTTGQ
EIIRSFDGGTPDAFVAGVGTGGTLTGVGRALRKINPQVQIYALEAAESPMLKEGHGGKHKIQGISAGFIPDVLDTNLYQD
IIEVTSDQAIDMARHVSHEEGFLPGISAGANIFGAIEIAKKLGKGKSVVTVAPDNGERYLSTDLFKFED

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=307, Percent_Identity=40.7166123778502, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI295821200, Length=307, Percent_Identity=40.7166123778502, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI4557415, Length=307, Percent_Identity=40.7166123778502, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1788754, Length=305, Percent_Identity=50.1639344262295, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI2367138, Length=294, Percent_Identity=41.8367346938776, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17535051, Length=299, Percent_Identity=49.4983277591973, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=52.9605263157895, Blast_Score=268, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17562970, Length=298, Percent_Identity=48.6577181208054, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=49.1467576791809, Blast_Score=261, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI17561720, Length=297, Percent_Identity=45.7912457912458, Blast_Score=220, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=49.0654205607477, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=37.5796178343949, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25147552, Length=309, Percent_Identity=36.5695792880259, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=39.8305084745763, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321594, Length=319, Percent_Identity=37.9310344827586, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=36.4197530864198, Blast_Score=146, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24643623, Length=306, Percent_Identity=39.8692810457516, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI20129101, Length=306, Percent_Identity=39.8692810457516, Blast_Score=200, Evalue=9e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32719; Mature: 32719

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTAADNITGLIGNTPLLKLNRVVPEDAADVYVKLEFFNPGGSVKDRIALAMIEDAEYKG
CCCCCCCCEEECCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCE
VLKPGGTIVEPTSGNTGIGLALVAAAKGYHLIITMPETMSVERRALMRGYGAELILTPGA
EECCCCEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCC
DGMPGAIKKAQALSKENGYFLPMQFQNPANPDVHERTTGQEIIRSFDGGTPDAFVAGVGT
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHCCCCCCCEEEECCC
GGTLTGVGRALRKINPQVQIYALEAAESPMLKEGHGGKHKIQGISAGFIPDVLDTNLYQD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
IIEVTSDQAIDMARHVSHEEGFLPGISAGANIFGAIEIAKKLGKGKSVVTVAPDNGERYL
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEE
STDLFKFED
ECCCEEECC
>Mature Secondary Structure
MVTAADNITGLIGNTPLLKLNRVVPEDAADVYVKLEFFNPGGSVKDRIALAMIEDAEYKG
CCCCCCCCEEECCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCEEEEEEEECCCCCE
VLKPGGTIVEPTSGNTGIGLALVAAAKGYHLIITMPETMSVERRALMRGYGAELILTPGA
EECCCCEEEECCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCEEEECCCC
DGMPGAIKKAQALSKENGYFLPMQFQNPANPDVHERTTGQEIIRSFDGGTPDAFVAGVGT
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHCCCCCCCEEEECCC
GGTLTGVGRALRKINPQVQIYALEAAESPMLKEGHGGKHKIQGISAGFIPDVLDTNLYQD
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHH
IIEVTSDQAIDMARHVSHEEGFLPGISAGANIFGAIEIAKKLGKGKSVVTVAPDNGERYL
HHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEE
STDLFKFED
ECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA