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The map label for this gene is metA [H]

Identifier: 116494038

GI number: 116494038

Start: 497036

End: 497851

Strand: Direct

Name: metA [H]

Synonym: LSEI_0479

Alternate gene names: 116494038

Gene position: 497036-497851 (Clockwise)

Preceding gene: 116494037

Following gene: 116494039

Centisome position: 17.17

GC content: 46.69

Gene sequence:

>816_bases
TTGGAAAAATCGCCACTCAAGATCGGTATTTTGAACGTCATGCATGATAAGGCTGACACCAAAACTCGGCTACAGCATGT
TTTGACGCACACTGCCATACCGGTCGATTTGCATTTTTACTACCCCATGACCCATTACGCCGGACGGACGGTGCCGGAAG
CGGTCAGTTCAATCCTCGATCCGCTGGACATTCATGAGGTGGCGACGATGGATGGGTTCATCATCACCGGTTCGCCGATT
GAAACCTTGGAATTTGATCAAGTGCATTATATCGCCGAAGTTCGAACCTTGTTGAAAACGTTAAGTCAGCATGTCCCTAA
TCAGCTCTATCTTTGCTGGGGTGGGATGGTTGCATTGAATTACTTTTTTGGCATCAGCAAGCTAATCTTGCCGCATAAAC
TTTTTGGCGTTTATCCGCAAACCATTCTTGAGCCGCACCCACTTTTGAAGGGACTCAAAAATGACTTCAAGTCGCCGCAT
GCACGCTATGCCGAAATGGATGTCCGCGGTATTCATGCCGATCCGCGGCTCACGATTAACGCGACAACGACGAAGGGCAA
ACTCTTCATGGCGACCGAACCGACTGATACGCAAACTTTCGTCTTTTCACACATCGAATACGATCGGTGGGGATTGGATT
CAGAATACAAGCGTGAAGTCGCTGCTCATCCAGAGATCGACTATGTTCGCGCTAAACATTATTATCATCACAAAAATGAT
TACGATCATCCCAAGTTCAATTGGAAGAAAACGCAACGAACGATTTTTGACAATTGGATTCAACATGTCGCGGATCATCG
CAATGACAATCATTAG

Upstream 100 bases:

>100_bases
TGTCCGGGCAAATGACCACATGTGGGGTTTCTTACCCACGGTCACACGCAAAGTTTCTTTTAAGGTTAATTAGTCATTAT
GAGGGAATAGGAGGTGAACC

Downstream 100 bases:

>100_bases
GAAAATCAATCAACTAATATGGAGGCTTTTAATATGGTGACAGCAGCAGATAATATTACCGGTTTGATTGGCAACACCCC
TTTGCTTAAGCTAAATCGTG

Product: homoserine trans-succinylase

Products: NA

Alternate protein names: Homoserine O-transsuccinylase; HTS [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MEKSPLKIGILNVMHDKADTKTRLQHVLTHTAIPVDLHFYYPMTHYAGRTVPEAVSSILDPLDIHEVATMDGFIITGSPI
ETLEFDQVHYIAEVRTLLKTLSQHVPNQLYLCWGGMVALNYFFGISKLILPHKLFGVYPQTILEPHPLLKGLKNDFKSPH
ARYAEMDVRGIHADPRLTINATTTKGKLFMATEPTDTQTFVFSHIEYDRWGLDSEYKREVAAHPEIDYVRAKHYYHHKND
YDHPKFNWKKTQRTIFDNWIQHVADHRNDNH

Sequences:

>Translated_271_residues
MEKSPLKIGILNVMHDKADTKTRLQHVLTHTAIPVDLHFYYPMTHYAGRTVPEAVSSILDPLDIHEVATMDGFIITGSPI
ETLEFDQVHYIAEVRTLLKTLSQHVPNQLYLCWGGMVALNYFFGISKLILPHKLFGVYPQTILEPHPLLKGLKNDFKSPH
ARYAEMDVRGIHADPRLTINATTTKGKLFMATEPTDTQTFVFSHIEYDRWGLDSEYKREVAAHPEIDYVRAKHYYHHKND
YDHPKFNWKKTQRTIFDNWIQHVADHRNDNH
>Mature_271_residues
MEKSPLKIGILNVMHDKADTKTRLQHVLTHTAIPVDLHFYYPMTHYAGRTVPEAVSSILDPLDIHEVATMDGFIITGSPI
ETLEFDQVHYIAEVRTLLKTLSQHVPNQLYLCWGGMVALNYFFGISKLILPHKLFGVYPQTILEPHPLLKGLKNDFKSPH
ARYAEMDVRGIHADPRLTINATTTKGKLFMATEPTDTQTFVFSHIEYDRWGLDSEYKREVAAHPEIDYVRAKHYYHHKND
YDHPKFNWKKTQRTIFDNWIQHVADHRNDNH

Specific function: Methionine biosynthesis; HTS variant; first step. [C]

COG id: COG1897

COG function: function code E; Homoserine trans-succinylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HTS family [H]

Homologues:

Organism=Escherichia coli, GI1790443, Length=264, Percent_Identity=30.6818181818182, Blast_Score=137, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005697
- ProDom:   PD037892 [H]

Pfam domain/function: PF04204 HTS [H]

EC number: =2.3.1.46 [H]

Molecular weight: Translated: 31494; Mature: 31494

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSPLKIGILNVMHDKADTKTRLQHVLTHTAIPVDLHFYYPMTHYAGRTVPEAVSSILD
CCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEECCHHHHCCCCHHHHHHHHHC
PLDIHEVATMDGFIITGSPIETLEFDQVHYIAEVRTLLKTLSQHVPNQLYLCWGGMVALN
CHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
YFFGISKLILPHKLFGVYPQTILEPHPLLKGLKNDFKSPHARYAEMDVRGIHADPRLTIN
HHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEE
ATTTKGKLFMATEPTDTQTFVFSHIEYDRWGLDSEYKREVAAHPEIDYVRAKHYYHHKND
EECCCCEEEEEECCCCCCEEEEECCCHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCC
YDHPKFNWKKTQRTIFDNWIQHVADHRNDNH
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKSPLKIGILNVMHDKADTKTRLQHVLTHTAIPVDLHFYYPMTHYAGRTVPEAVSSILD
CCCCCEEEEHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEECCHHHHCCCCHHHHHHHHHC
PLDIHEVATMDGFIITGSPIETLEFDQVHYIAEVRTLLKTLSQHVPNQLYLCWGGMVALN
CHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
YFFGISKLILPHKLFGVYPQTILEPHPLLKGLKNDFKSPHARYAEMDVRGIHADPRLTIN
HHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHCCCCHHHHHCCEEEECCCCEEEEE
ATTTKGKLFMATEPTDTQTFVFSHIEYDRWGLDSEYKREVAAHPEIDYVRAKHYYHHKND
EECCCCEEEEEECCCCCCEEEEECCCHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCC
YDHPKFNWKKTQRTIFDNWIQHVADHRNDNH
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA