BacMap

Definition	Lactobacillus casei ATCC 334, complete genome.
Accession	NC_008526
Length	2,895,264

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The map label for this gene is yqhE [C]

Identifier: 116494006

GI number: 116494006

Start: 447819

End: 448685

Strand: Direct

Name: yqhE [C]

Synonym: LSEI_0443

Alternate gene names: 116494006

Gene position: 447819-448685 (Clockwise)

Preceding gene: 116494004

Following gene: 116494008

Centisome position: 15.47

GC content: 45.67

Gene sequence:

>867_bases
ATGTTAGAAGCAACTTATACATTAGCAAATGGCGTACAGATTCCTAAAATCGGGTTTGGCACTTGGATGATTGATGCCGA
TGCTGATGCTGCGAAAGCTGTTCATGAAGCCATTGACGCCGGTTATCGCCATATTGATACCGCGGAGGCATATGGTAATG
AAGTCGGTGTTGGTGAAGGTGTGCGTGCGTCTGGGATCGATCGCAAAGACATTTTTGTGAATACAAAACTGGATGCCGAA
ATCAAAAACCATGATGAAGCGGTAAAAGCGATTGATGACTCATTGCAGAAGCTGAACTTGGACTACATTGACATGATGAT
TATTCATGCACCGAAACCCTGGGCTAAGTACGATGAACCCAATCGTTATTTTGAGGGCAATTTAGAAGCGTGGCGCGCCT
TGGAGGAGGCATACAAAGCAGGCAAACTCCGCGCAATCGGCGTTTCTAACTTTGACCCGACTGATTTGGAAAATATCATC
AAGCATGCCAAAATCGCGCCAATGGTGGATCAAGTTTTGGCCCACATCACGCAGACGCCATTCAAAACGATTGATTATGC
TAAGGCGCACAACATTTTGGTTGAAGCTTACTCACCATTTGGTCATGGCGAAATGTTCAAAAACACCGCAATTCAAGCCA
TGGCTGCTAAGCACCACGTTTCGGTTGCTCAGCTTGGTATGCGGTATCTGTTGCAACTGGGCCTGCTACCATTGCCAAAA
ACGGTCAACCCAGAACATATGAAGGCGAATGCTGATGTCGATTTTTCAATTGACGATGCTGACATGACCACACTCAAGCA
GATGAAGCCTTTGACAGATTATGGTCAGTTTCAGAAGTTCCCGGTTTACAGTGATCGTTTGAGTTAG

Upstream 100 bases:

>100_bases
CTTGATCAAGCGTAAGGAAAATCCACTCGGTCACGAACGTTTGGTAGTGATGACTGGGATGAAAGATCAAGGCAGCATTA
TTTGTCGAGGAGGGACTATT

Downstream 100 bases:

>100_bases
TGCCGACACACTGCTGGTAATATCAGAAAATTGATACGACAAAATGAGGTGCTGATAAAAAGCGCCTCATTTTTGCTATC
CTTGTGGGGATTTATCAAAA

Product: aldo/keto reductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLEATYTLANGVQIPKIGFGTWMIDADADAAKAVHEAIDAGYRHIDTAEAYGNEVGVGEGVRASGIDRKDIFVNTKLDAE
IKNHDEAVKAIDDSLQKLNLDYIDMMIIHAPKPWAKYDEPNRYFEGNLEAWRALEEAYKAGKLRAIGVSNFDPTDLENII
KHAKIAPMVDQVLAHITQTPFKTIDYAKAHNILVEAYSPFGHGEMFKNTAIQAMAAKHHVSVAQLGMRYLLQLGLLPLPK
TVNPEHMKANADVDFSIDDADMTTLKQMKPLTDYGQFQKFPVYSDRLS

Sequences:

>Translated_288_residues
MLEATYTLANGVQIPKIGFGTWMIDADADAAKAVHEAIDAGYRHIDTAEAYGNEVGVGEGVRASGIDRKDIFVNTKLDAE
IKNHDEAVKAIDDSLQKLNLDYIDMMIIHAPKPWAKYDEPNRYFEGNLEAWRALEEAYKAGKLRAIGVSNFDPTDLENII
KHAKIAPMVDQVLAHITQTPFKTIDYAKAHNILVEAYSPFGHGEMFKNTAIQAMAAKHHVSVAQLGMRYLLQLGLLPLPK
TVNPEHMKANADVDFSIDDADMTTLKQMKPLTDYGQFQKFPVYSDRLS
>Mature_288_residues
MLEATYTLANGVQIPKIGFGTWMIDADADAAKAVHEAIDAGYRHIDTAEAYGNEVGVGEGVRASGIDRKDIFVNTKLDAE
IKNHDEAVKAIDDSLQKLNLDYIDMMIIHAPKPWAKYDEPNRYFEGNLEAWRALEEAYKAGKLRAIGVSNFDPTDLENII
KHAKIAPMVDQVLAHITQTPFKTIDYAKAHNILVEAYSPFGHGEMFKNTAIQAMAAKHHVSVAQLGMRYLLQLGLLPLPK
TVNPEHMKANADVDFSIDDADMTTLKQMKPLTDYGQFQKFPVYSDRLS

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=32.8767123287671, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4502049, Length=284, Percent_Identity=34.8591549295775, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=33.7883959044369, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI24497577, Length=294, Percent_Identity=30.2721088435374, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI5174391, Length=294, Percent_Identity=30.2721088435374, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI93277124, Length=288, Percent_Identity=30.5555555555556, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI300116273, Length=280, Percent_Identity=34.6428571428571, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI24497583, Length=287, Percent_Identity=30.3135888501742, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI5453543, Length=288, Percent_Identity=31.9444444444444, Blast_Score=131, Evalue=6e-31,
Organism=Homo sapiens, GI310109920, Length=288, Percent_Identity=31.9444444444444, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI45446745, Length=287, Percent_Identity=31.7073170731707, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4503285, Length=287, Percent_Identity=31.7073170731707, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI24497585, Length=287, Percent_Identity=31.0104529616725, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=32.319391634981, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI310109922, Length=279, Percent_Identity=31.1827956989247, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI300116271, Length=284, Percent_Identity=29.5774647887324, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI310109926, Length=111, Percent_Identity=37.8378378378378, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI207028673, Length=110, Percent_Identity=37.2727272727273, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI87082198, Length=264, Percent_Identity=31.8181818181818, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786400, Length=241, Percent_Identity=31.5352697095436, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=28.2608695652174, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994888, Length=269, Percent_Identity=23.0483271375465, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17564128, Length=294, Percent_Identity=32.9931972789116, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=33.7979094076655, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17561298, Length=279, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17552492, Length=262, Percent_Identity=31.2977099236641, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71998625, Length=266, Percent_Identity=32.7067669172932, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17537077, Length=286, Percent_Identity=32.1678321678322, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17537075, Length=286, Percent_Identity=32.1678321678322, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17550248, Length=291, Percent_Identity=30.2405498281787, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.1904761904762, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538386, Length=280, Percent_Identity=28.9285714285714, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17561300, Length=251, Percent_Identity=32.2709163346614, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17562292, Length=289, Percent_Identity=26.2975778546713, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6320576, Length=311, Percent_Identity=34.0836012861736, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324694, Length=286, Percent_Identity=34.965034965035, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319625, Length=308, Percent_Identity=32.7922077922078, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6322556, Length=275, Percent_Identity=32.3636363636364, Blast_Score=141, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321896, Length=301, Percent_Identity=29.5681063122924, Blast_Score=139, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320079, Length=277, Percent_Identity=32.4909747292419, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24657054, Length=288, Percent_Identity=36.1111111111111, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI20129731, Length=288, Percent_Identity=32.9861111111111, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21356425, Length=302, Percent_Identity=32.4503311258278, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24644950, Length=306, Percent_Identity=31.0457516339869, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI221378297, Length=327, Percent_Identity=29.3577981651376, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24663317, Length=289, Percent_Identity=32.1799307958478, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24662789, Length=291, Percent_Identity=30.9278350515464, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45553081, Length=290, Percent_Identity=30.6896551724138, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24662781, Length=280, Percent_Identity=31.7857142857143, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24662785, Length=280, Percent_Identity=31.4285714285714, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281366140, Length=251, Percent_Identity=30.6772908366534, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 32091; Mature: 32091

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEATYTLANGVQIPKIGFGTWMIDADADAAKAVHEAIDAGYRHIDTAEAYGNEVGVGEG
CCCCEEEHHCCEECCEECCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
VRASGIDRKDIFVNTKLDAEIKNHDEAVKAIDDSLQKLNLDYIDMMIIHAPKPWAKYDEP
CCCCCCCCCCEEEECEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
NRYFEGNLEAWRALEEAYKAGKLRAIGVSNFDPTDLENIIKHAKIAPMVDQVLAHITQTP
CCEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FKTIDYAKAHNILVEAYSPFGHGEMFKNTAIQAMAAKHHVSVAQLGMRYLLQLGLLPLPK
HHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TVNPEHMKANADVDFSIDDADMTTLKQMKPLTDYGQFQKFPVYSDRLS
CCCHHHEECCCCCEEEECCCHHHHHHHHCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLEATYTLANGVQIPKIGFGTWMIDADADAAKAVHEAIDAGYRHIDTAEAYGNEVGVGEG
CCCCEEEHHCCEECCEECCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCC
VRASGIDRKDIFVNTKLDAEIKNHDEAVKAIDDSLQKLNLDYIDMMIIHAPKPWAKYDEP
CCCCCCCCCCEEEECEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCC
NRYFEGNLEAWRALEEAYKAGKLRAIGVSNFDPTDLENIIKHAKIAPMVDQVLAHITQTP
CCEECCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FKTIDYAKAHNILVEAYSPFGHGEMFKNTAIQAMAAKHHVSVAQLGMRYLLQLGLLPLPK
HHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TVNPEHMKANADVDFSIDDADMTTLKQMKPLTDYGQFQKFPVYSDRLS
CCCHHHEECCCCCEEEECCCHHHHHHHHCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA