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The map label for this gene is ydaP [H]

Identifier: 116493997

GI number: 116493997

Start: 437030

End: 438763

Strand: Direct

Name: ydaP [H]

Synonym: LSEI_0433

Alternate gene names: 116493997

Gene position: 437030-438763 (Clockwise)

Preceding gene: 116493995

Following gene: 116494000

Centisome position: 15.09

GC content: 47.35

Gene sequence:

>1734_bases
ATGGCAACAAAAGCAGCAGATCAGTTAGTCAGCGTTTTGATGGATTGGCAAGTTAAACACGTTTTTGGATTGCCTGGTGA
TTCAATCGATACCACAGTTGATGCCTTACGTCGACATCAGGATAAGATCAAGTTTGTGCAGGTGCGGCATGAGGAAGTCG
CCGCTTTGTCCGCAGCGGCAACTGCTAAGTTAACAGGCGGTTTGGGTGTTTGTCTCTCCATTGGCGGCCCTGGCGCCATT
CATCTATTAAATGGTTTGTATGATGCCAAAATGGATCATGTCCCGGTTCTGGCCCTTCTCGGTCAGGTGACGACGAGCAA
CATGAATGAAGGCTTTTTCCAAGAAGTGAATACACCGAAGCTTTTTGACGATGTGGCGGTTTATAACAAAACTGTCATGG
CATCGGATAACTTAGGACAAATTGTCGATACGGCGATTCGCACAGCCTTTACAGAAAAAGGGGTGGCAGTTTTAACCATT
CCAGATGATTTGCCAGCTCAAAAAGAAACTGCTAGTTATCAGGACAGCGCTGCTGCTTTTGCCTTGAATGTGCCGAAAGT
TGATCCTAAACAGTTAGAGGACGTTGCTTCGATGCTGCAACAGGCACAAAAACCACTTGCGTTGATCGGTCGCGGTGCTG
AGCATGCAGGTGCGTTGGTTCAAAAGTTTGTCGAGGCCAATCACATACCGTTTATTCAAACGATGCCAGCAAAAGGCACT
ATCGCCGATGATCATCCCAATAGTTTGGGCAATGTCGGTAAACTAGGTACGAAGCCGGCATATGAGGCGATGAAAGCGAC
TGATCTCCTCTTCATGATCGGGACGAATTATCCATATACGCCTTATTTGCCAGCCGAAGGTCAAGCTAAGTGCGTTCAAA
TCGACACGCGCCCAGAAAACTTGGGCAAACGTTATTCAGTTGATGTTGCTGTTGACGGCGACGTTGGCACCTTTTTCACC
GAACTGAATGCGAAGGGCTCCTTGCGTGATGATGATCGTTTTCTGAAAGCTTGCCAGAAGAATATGGAGAGCTGGAATAA
GTGGATGAGTGAAAAGCGCTTGCTGGCTACTAATCCGGCATCGCCTGAAGCAGTCTTCGCGACGATTGATCAAACCGCGC
CAAAAGATGCGATTTACTCGATTGATGTCGGTACATCCACATCATGGGGTGCCCGTTTCTTAAACGTTCAGCCAACGCAA
AAGTATACAATTTCCGCATGGTTGGGAACCATGGGCTGTGGCTTGCCAGGCGCGATTGCGGGTGCTGAGGCTTTTCCAAA
GCGGCAAAATATCAGTGTCGCAGGGGATGGTGCCTTCGCGATGGTCATGCAAGACTTTGTCACAGCAGTCAAGTACAAGT
TGCCGATCATCATGGTTGTCATGAATAATCAGAAGTTGGCATTTATTGAATATGAGCAGCAAAGTGCCGGTCAGCTGAAC
TATGAAATTGATTTGGCTGATATGGACTATGCCAAAATTGCTGAAGCAGCAGGCGGCATTGGCTACACCGCTCGCAGCAA
TGACGAATTCAAAAATGCATTAGCCAAGGCATACAAAGAGACCGATAAACCGGTTCTGATCAATACCTACGTCCAAGACG
ATGCACCGCTTCCAGGCAAAATCGTTGGTGATGAAGCAAAGGGTTACATGAAGTACGGTTCCGAATATCTTGAGAATTAT
TGGAAGATTCCGTCCATGCCGCCATTGAAGGATATTATGCGGCAATTCTTCTAA

Upstream 100 bases:

>100_bases
GCAGTTGACCATGCCGTTGAAAGAACTAAGGTGGAGTTGTCAATAAATTGATAACAATTGAGTTGTTTGCATGACGAAAT
GCGATAAAGGAGATTTTGAT

Downstream 100 bases:

>100_bases
TTTTCGATTAGCTTGTATCAAAAATAAATGGCTTCAATAAAAAATCCCCACTCCGAGTCACGTCCGAAGTGAGGATTTTT
TTTACTTAGAGGGTCTCCCG

Product: pyruvate oxidase

Products: acetyl phosphate; CO2; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MATKAADQLVSVLMDWQVKHVFGLPGDSIDTTVDALRRHQDKIKFVQVRHEEVAALSAAATAKLTGGLGVCLSIGGPGAI
HLLNGLYDAKMDHVPVLALLGQVTTSNMNEGFFQEVNTPKLFDDVAVYNKTVMASDNLGQIVDTAIRTAFTEKGVAVLTI
PDDLPAQKETASYQDSAAAFALNVPKVDPKQLEDVASMLQQAQKPLALIGRGAEHAGALVQKFVEANHIPFIQTMPAKGT
IADDHPNSLGNVGKLGTKPAYEAMKATDLLFMIGTNYPYTPYLPAEGQAKCVQIDTRPENLGKRYSVDVAVDGDVGTFFT
ELNAKGSLRDDDRFLKACQKNMESWNKWMSEKRLLATNPASPEAVFATIDQTAPKDAIYSIDVGTSTSWGARFLNVQPTQ
KYTISAWLGTMGCGLPGAIAGAEAFPKRQNISVAGDGAFAMVMQDFVTAVKYKLPIIMVVMNNQKLAFIEYEQQSAGQLN
YEIDLADMDYAKIAEAAGGIGYTARSNDEFKNALAKAYKETDKPVLINTYVQDDAPLPGKIVGDEAKGYMKYGSEYLENY
WKIPSMPPLKDIMRQFF

Sequences:

>Translated_577_residues
MATKAADQLVSVLMDWQVKHVFGLPGDSIDTTVDALRRHQDKIKFVQVRHEEVAALSAAATAKLTGGLGVCLSIGGPGAI
HLLNGLYDAKMDHVPVLALLGQVTTSNMNEGFFQEVNTPKLFDDVAVYNKTVMASDNLGQIVDTAIRTAFTEKGVAVLTI
PDDLPAQKETASYQDSAAAFALNVPKVDPKQLEDVASMLQQAQKPLALIGRGAEHAGALVQKFVEANHIPFIQTMPAKGT
IADDHPNSLGNVGKLGTKPAYEAMKATDLLFMIGTNYPYTPYLPAEGQAKCVQIDTRPENLGKRYSVDVAVDGDVGTFFT
ELNAKGSLRDDDRFLKACQKNMESWNKWMSEKRLLATNPASPEAVFATIDQTAPKDAIYSIDVGTSTSWGARFLNVQPTQ
KYTISAWLGTMGCGLPGAIAGAEAFPKRQNISVAGDGAFAMVMQDFVTAVKYKLPIIMVVMNNQKLAFIEYEQQSAGQLN
YEIDLADMDYAKIAEAAGGIGYTARSNDEFKNALAKAYKETDKPVLINTYVQDDAPLPGKIVGDEAKGYMKYGSEYLENY
WKIPSMPPLKDIMRQFF
>Mature_576_residues
ATKAADQLVSVLMDWQVKHVFGLPGDSIDTTVDALRRHQDKIKFVQVRHEEVAALSAAATAKLTGGLGVCLSIGGPGAIH
LLNGLYDAKMDHVPVLALLGQVTTSNMNEGFFQEVNTPKLFDDVAVYNKTVMASDNLGQIVDTAIRTAFTEKGVAVLTIP
DDLPAQKETASYQDSAAAFALNVPKVDPKQLEDVASMLQQAQKPLALIGRGAEHAGALVQKFVEANHIPFIQTMPAKGTI
ADDHPNSLGNVGKLGTKPAYEAMKATDLLFMIGTNYPYTPYLPAEGQAKCVQIDTRPENLGKRYSVDVAVDGDVGTFFTE
LNAKGSLRDDDRFLKACQKNMESWNKWMSEKRLLATNPASPEAVFATIDQTAPKDAIYSIDVGTSTSWGARFLNVQPTQK
YTISAWLGTMGCGLPGAIAGAEAFPKRQNISVAGDGAFAMVMQDFVTAVKYKLPIIMVVMNNQKLAFIEYEQQSAGQLNY
EIDLADMDYAKIAEAAGGIGYTARSNDEFKNALAKAYKETDKPVLINTYVQDDAPLPGKIVGDEAKGYMKYGSEYLENYW
KIPSMPPLKDIMRQFF

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=552, Percent_Identity=26.0869565217391, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI21361361, Length=576, Percent_Identity=22.9166666666667, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=31.1711711711712, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87081685, Length=540, Percent_Identity=28.3333333333333, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1790104, Length=490, Percent_Identity=28.9795918367347, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786717, Length=531, Percent_Identity=26.3653483992467, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788716, Length=548, Percent_Identity=24.6350364963504, Blast_Score=126, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=547, Percent_Identity=26.3254113345521, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=547, Percent_Identity=26.3254113345521, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=552, Percent_Identity=25.1811594202899, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=25.8232235701906, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=485, Percent_Identity=24.3298969072165, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321524, Length=486, Percent_Identity=22.8395061728395, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=26.3066202090592, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.3.3

Molecular weight: Translated: 62754; Mature: 62623

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKAADQLVSVLMDWQVKHVFGLPGDSIDTTVDALRRHQDKIKFVQVRHEEVAALSAAA
CCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHH
TAKLTGGLGVCLSIGGPGAIHLLNGLYDAKMDHVPVLALLGQVTTSNMNEGFFQEVNTPK
HHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCH
LFDDVAVYNKTVMASDNLGQIVDTAIRTAFTEKGVAVLTIPDDLPAQKETASYQDSAAAF
HHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCCCCEEE
ALNVPKVDPKQLEDVASMLQQAQKPLALIGRGAEHAGALVQKFVEANHIPFIQTMPAKGT
EEECCCCCHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCC
IADDHPNSLGNVGKLGTKPAYEAMKATDLLFMIGTNYPYTPYLPAEGQAKCVQIDTRPEN
CCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCEEEEEECCHHH
LGKRYSVDVAVDGDVGTFFTELNAKGSLRDDDRFLKACQKNMESWNKWMSEKRLLATNPA
CCCEEEEEEEECCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
SPEAVFATIDQTAPKDAIYSIDVGTSTSWGARFLNVQPTQKYTISAWLGTMGCGLPGAIA
CCCEEEEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCCCCHHC
GAEAFPKRQNISVAGDGAFAMVMQDFVTAVKYKLPIIMVVMNNQKLAFIEYEQQSAGQLN
CHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCEE
YEIDLADMDYAKIAEAAGGIGYTARSNDEFKNALAKAYKETDKPVLINTYVQDDAPLPGK
EEEEECCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
IVGDEAKGYMKYGSEYLENYWKIPSMPPLKDIMRQFF
EECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
ATKAADQLVSVLMDWQVKHVFGLPGDSIDTTVDALRRHQDKIKFVQVRHEEVAALSAAA
CCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHH
TAKLTGGLGVCLSIGGPGAIHLLNGLYDAKMDHVPVLALLGQVTTSNMNEGFFQEVNTPK
HHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHCCCCH
LFDDVAVYNKTVMASDNLGQIVDTAIRTAFTEKGVAVLTIPDDLPAQKETASYQDSAAAF
HHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHCCCCCCCEEE
ALNVPKVDPKQLEDVASMLQQAQKPLALIGRGAEHAGALVQKFVEANHIPFIQTMPAKGT
EEECCCCCHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCC
IADDHPNSLGNVGKLGTKPAYEAMKATDLLFMIGTNYPYTPYLPAEGQAKCVQIDTRPEN
CCCCCCCCCCCCCCCCCCHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCEEEEEECCHHH
LGKRYSVDVAVDGDVGTFFTELNAKGSLRDDDRFLKACQKNMESWNKWMSEKRLLATNPA
CCCEEEEEEEECCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
SPEAVFATIDQTAPKDAIYSIDVGTSTSWGARFLNVQPTQKYTISAWLGTMGCGLPGAIA
CCCEEEEEECCCCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCCCCHHC
GAEAFPKRQNISVAGDGAFAMVMQDFVTAVKYKLPIIMVVMNNQKLAFIEYEQQSAGQLN
CHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCCEE
YEIDLADMDYAKIAEAAGGIGYTARSNDEFKNALAKAYKETDKPVLINTYVQDDAPLPGK
EEEEECCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCC
IVGDEAKGYMKYGSEYLENYWKIPSMPPLKDIMRQFF
EECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; phosphate; O2; H2O

Specific reaction: pyruvate + phosphate + O2 + H2O = acetyl phosphate + CO2 + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]