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The map label for this gene is tag [H]

Identifier: 116493995

GI number: 116493995

Start: 434915

End: 435490

Strand: Direct

Name: tag [H]

Synonym: LSEI_0431

Alternate gene names: 116493995

Gene position: 434915-435490 (Clockwise)

Preceding gene: 116493994

Following gene: 116493997

Centisome position: 15.02

GC content: 47.4

Gene sequence:

>576_bases
ATGCTGGCTGAAAAACACCGTTGCGCATGGGGACAAACCAATAATGACTTGATGCGCGAGTATCATGATGAAGAATGGGG
GCGGCCCAGCCATGACAGCCGCCACTTATTTGAGCTTTTGTCGCTTGAAATCATGCAAGCAGGTTTGAGCTGGCAGACCG
TTTTGAATAAACGCGCTGCTTTCAAACAGGCTTTTGCTAACTTTGACTATCGGCAGGTGCAGCAGATGGCACCGAAGATT
CCGGTGCTCTTAGAAAATACGCAAATTATTCGGAATCGGCTGAAAGTGATGGCGATCATTAACAATGCGCGCGTGATCGC
ACAGTTAGCTGCCAAAGGCGAGGACTTTGATCATTACGTGTGGGCCTTTGTCAACAATCAGCCGATTCGGCATCATATTA
CCAGTCATGATCAGGTGCCTAACACAACCGACTTGGCCAAGCACATGAGCAAGCAAATGAAGGCAGATGGCTTTGCATTT
ACTGGTCCCGTCGTTATCTACTCATTTATGCAGGCTGCAGGGTTGGTCAATGATCATGAAGCCGACTGTTTTGTTAATCA
AACACTTGATGAGTAA

Upstream 100 bases:

>100_bases
GTCATGGCTAAACTTGTCGCTGAGCATCAACTTGCTTTGACGCAGAATCGACGCGTGCAAGTGACCCAGCCGATTAAGAT
ACAACCGCTGGAGGTACGAT

Downstream 100 bases:

>100_bases
AGAAATATGTATGAAAAAGCCCACTCGCTGAAATTTGCGAGTGGGCTTTTGAATGGCACTTAATGAAAAAATTAAAGTGC
TTCTGGAACCATCTTAACTG

Product: 3-methyladenine DNA glycosylase

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosidase; TAG [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MLAEKHRCAWGQTNNDLMREYHDEEWGRPSHDSRHLFELLSLEIMQAGLSWQTVLNKRAAFKQAFANFDYRQVQQMAPKI
PVLLENTQIIRNRLKVMAIINNARVIAQLAAKGEDFDHYVWAFVNNQPIRHHITSHDQVPNTTDLAKHMSKQMKADGFAF
TGPVVIYSFMQAAGLVNDHEADCFVNQTLDE

Sequences:

>Translated_191_residues
MLAEKHRCAWGQTNNDLMREYHDEEWGRPSHDSRHLFELLSLEIMQAGLSWQTVLNKRAAFKQAFANFDYRQVQQMAPKI
PVLLENTQIIRNRLKVMAIINNARVIAQLAAKGEDFDHYVWAFVNNQPIRHHITSHDQVPNTTDLAKHMSKQMKADGFAF
TGPVVIYSFMQAAGLVNDHEADCFVNQTLDE
>Mature_191_residues
MLAEKHRCAWGQTNNDLMREYHDEEWGRPSHDSRHLFELLSLEIMQAGLSWQTVLNKRAAFKQAFANFDYRQVQQMAPKI
PVLLENTQIIRNRLKVMAIINNARVIAQLAAKGEDFDHYVWAFVNNQPIRHHITSHDQVPNTTDLAKHMSKQMKADGFAF
TGPVVIYSFMQAAGLVNDHEADCFVNQTLDE

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=178, Percent_Identity=44.9438202247191, Blast_Score=161, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 22020; Mature: 22020

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEKHRCAWGQTNNDLMREYHDEEWGRPSHDSRHLFELLSLEIMQAGLSWQTVLNKRAA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FKQAFANFDYRQVQQMAPKIPVLLENTQIIRNRLKVMAIINNARVIAQLAAKGEDFDHYV
HHHHHHCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
WAFVNNQPIRHHITSHDQVPNTTDLAKHMSKQMKADGFAFTGPVVIYSFMQAAGLVNDHE
EEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
ADCFVNQTLDE
CHHHHHCCCCC
>Mature Secondary Structure
MLAEKHRCAWGQTNNDLMREYHDEEWGRPSHDSRHLFELLSLEIMQAGLSWQTVLNKRAA
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
FKQAFANFDYRQVQQMAPKIPVLLENTQIIRNRLKVMAIINNARVIAQLAAKGEDFDHYV
HHHHHHCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
WAFVNNQPIRHHITSHDQVPNTTDLAKHMSKQMKADGFAFTGPVVIYSFMQAAGLVNDHE
EEEECCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
ADCFVNQTLDE
CHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]