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The map label for this gene is exp5 [H]

Identifier: 116493940

GI number: 116493940

Start: 375184

End: 375672

Strand: Direct

Name: exp5 [H]

Synonym: LSEI_0374

Alternate gene names: 116493940

Gene position: 375184-375672 (Clockwise)

Preceding gene: 116493939

Following gene: 116493941

Centisome position: 12.96

GC content: 41.51

Gene sequence:

>489_bases
ATGTTTGGACTATTTAAAGCTAAGAAAAAAACAGGACAGCCGATTATTGCCCCTGTCACGGGCATCCTTATGCCACTCGA
TGATGTGACGGATGACGTTTTTTCACAAAAAATGATGGGAGACGGCTTTGCGATTCAACCAGAAGAATCGCAAATTGTTT
CTCCGGTTTCTGGTACTGTGTCAACGGTTTTCCCTACGAAACATGCGATTGGTATCACGACTCCAGAAGGTTTAGACGTG
CTCGTTCACATGGGCTTAGATACGGTCGAATTAGACGGGAAACCTTTTAAAGTTGATGTTGCTCTAAATGATGCTGTGAC
GGCGGGTCAACCACTTGCTACCATTGATCGTGAACAGATCAAAAAAAGTGGTTATGACGATACGATTGTCGTCATCTATA
CAAATATGGAAAAATTAAAGAACTTTCCAACAGTTGTTTCAAGTCAAATTACGCAGGGCCAACAAATTGGTGAACTGATA
TACGTTTAA

Upstream 100 bases:

>100_bases
AAATAACAAACGATAATGGCGGCTAACAATTGTGTAAAAATAAACGTTGAAACTTGCCCCGACATGTCGAATGAATCTTA
TTGGTAAGGAGTGAGTATTT

Downstream 100 bases:

>100_bases
AGAATCTGAATCATAACTTTTACCGACGCGATGATAGTCACATGGCCAATATCTGGGGGATTCTAATGACAGGGTTAATC
TTTGATTTTAATGGGACACT

Product: phosphotransferase system IIA component

Products: NA

Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MFGLFKAKKKTGQPIIAPVTGILMPLDDVTDDVFSQKMMGDGFAIQPEESQIVSPVSGTVSTVFPTKHAIGITTPEGLDV
LVHMGLDTVELDGKPFKVDVALNDAVTAGQPLATIDREQIKKSGYDDTIVVIYTNMEKLKNFPTVVSSQITQGQQIGELI
YV

Sequences:

>Translated_162_residues
MFGLFKAKKKTGQPIIAPVTGILMPLDDVTDDVFSQKMMGDGFAIQPEESQIVSPVSGTVSTVFPTKHAIGITTPEGLDV
LVHMGLDTVELDGKPFKVDVALNDAVTAGQPLATIDREQIKKSGYDDTIVVIYTNMEKLKNFPTVVSSQITQGQQIGELI
YV
>Mature_162_residues
MFGLFKAKKKTGQPIIAPVTGILMPLDDVTDDVFSQKMMGDGFAIQPEESQIVSPVSGTVSTVFPTKHAIGITTPEGLDV
LVHMGLDTVELDGKPFKVDVALNDAVTAGQPLATIDREQIKKSGYDDTIVVIYTNMEKLKNFPTVVSSQITQGQQIGELI
YV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790159, Length=146, Percent_Identity=36.3013698630137, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1786894, Length=140, Percent_Identity=35, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788757, Length=148, Percent_Identity=35.8108108108108, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011300 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17482; Mature: 17482

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLFKAKKKTGQPIIAPVTGILMPLDDVTDDVFSQKMMGDGFAIQPEESQIVSPVSGTV
CCCCCCCHHHCCCEEEECHHHHEECCHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCE
STVFPTKHAIGITTPEGLDVLVHMGLDTVELDGKPFKVDVALNDAVTAGQPLATIDREQI
EEECCCCCEEECCCCCCCEEEEECCCCEEEECCCEEEEEEEECCCCCCCCCCHHCCHHHH
KKSGYDDTIVVIYTNMEKLKNFPTVVSSQITQGQQIGELIYV
HHCCCCCEEEEEEECHHHHHCCCHHHHHHHHCCHHHHHEECC
>Mature Secondary Structure
MFGLFKAKKKTGQPIIAPVTGILMPLDDVTDDVFSQKMMGDGFAIQPEESQIVSPVSGTV
CCCCCCCHHHCCCEEEECHHHHEECCHHHHHHHHHHHHCCCCCEECCCCCCEECCCCCCE
STVFPTKHAIGITTPEGLDVLVHMGLDTVELDGKPFKVDVALNDAVTAGQPLATIDREQI
EEECCCCCEEECCCCCCCEEEEECCCCEEEECCCEEEEEEEECCCCCCCCCCHHCCHHHH
KKSGYDDTIVVIYTNMEKLKNFPTVVSSQITQGQQIGELIYV
HHCCCCCEEEEEEECHHHHHCCCHHHHHHHHCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11463916; 7934910 [H]