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The map label for this gene is rfbX [H]

Identifier: 116493809

GI number: 116493809

Start: 237943

End: 239382

Strand: Direct

Name: rfbX [H]

Synonym: LSEI_0238

Alternate gene names: 116493809

Gene position: 237943-239382 (Clockwise)

Preceding gene: 116493807

Following gene: 116493810

Centisome position: 8.22

GC content: 42.85

Gene sequence:

>1440_bases
ATGAAAGTAATCAAGAACTTTTTTTGGAATGCGGGTTATCAGGTCTTCGTCCTGATTGTGCCGCTGGTAACTGTACCTTA
TATTAATCGAGTACTAGGACCGACAGGCGTTGGGATAAATGCGTTCACTAACTCAATTGTCCAATACTTTATTCTCTTTG
GTAGTCTGGGTATTAACCTTTATGGGAATCGTGGCACAGCTTATCGAAGAGATGATCGAAAAGCGCTGACCACCTATTTT
TGGGAAGTCACCATACTTCGATTTGTGACGATTGGTATAGCTGTGCTTGCGTATCTCATGTTTATATTTGCCGTTGATGA
GTATCGTGTTTTTTATCTTGCTCAAGGCGTTATGCTTTTGGGAACAGCGTTTGATATTTCGTGGTTTTTCCAAGGATTAG
AAAACTTTCGGGTAACGGTGGTTCGTAATGTGTTGGTGCGGATCGCTTCCCTTATTCTGATTTTCCTGTTAGTGCATAAA
GCAGATGATACTGCATTGTATATTCTGATCATGTCTGGCTCACAGATGTTGGGGAATCTAACATTCTGGCCCTCATTACG
CGCAAATCTGACGCATTTTCCGAAACTGTCAAGCCTGAACATCTGGCAGCATATTAAACCGGCGTTCCTTCTATTGATTC
CGCAACTGGCGATTCAGATTTATGTTCAACTGAATAAAACGATGCTTGGAATTTTACAGGGTGTTACGGCATCTGGTTTT
TACGAAAGTTCGGACAAAATCATTAAAATGTTATTGGCTCTTGTGACAGCAACCGGCACCGTCTTATTGCCACATGTGGC
CCATTATTTTGCCCAAGGTGATCACGATGCCGTCAAACGCTCATTAGAAACATCGATGCACGTGATTTTGGTTATTGCTT
TTCCTTTAGCCTTTGGGATTGCGGCGGTTTCCACAACGTTTACTTATTATTTTTTTAGCACAAAGTTTATGCCCGTGGCA
CCCTTGATGGCAGCTGAAGCGATTGTCGTCATTCCGATTTCGATTGCGAGTGCCATTGGTGTGCAATATTTGCTGCCAAC
TAACCAAGTTAAGTCATATACTGTCTCAGTTATTTTGGGATCCATCGTTAATATTGTAGTGAATGTGCCTCTTATTCTGT
GGTTAGGAACAATGGGCGCTGTGATTGGCACTATCCTTTCCGAATCAGTCGTGACGATTTATCAGGTCTATGCTATTAAA
AATCAGCTTGATCTCAGAGGGTTGTTTAGTGAATCATGGAAGTATTGCCTCAGCGCGGTCGTGATGTTTGGTGTTGTAAA
AGGTCTTGAGATTGCCTGGTCCACCAGCTTGATCGGCCTAGTTGTTGAAGTTTTGATTGGTATGGTGGTGTACTTTGTGG
TGCTGTTGGGGTTACGACCGCACATTATCATTGGGTATGTTCGCCCATATGTCGATCAGATGCGTCGGCGTCTTCGTTAA

Upstream 100 bases:

>100_bases
TGATTCGATATCTGGTAATATTAGTAGGTGTTGATTAGTTAACAACCGAGCGCTATCGTTCCATCTCGACAGCGCGCAAA
TGCAAGTTTTGGAGATTTTT

Downstream 100 bases:

>100_bases
ACTGAATTAGATTCATTAAGGGGAGTTATATGATGAAGATTGGGTTGATCCTTAACTATCGAAATTATCGACAGACCATC
GCATGTTGCCGCAACTTAAT

Product: PST family polysaccharide transporter

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKVIKNFFWNAGYQVFVLIVPLVTVPYINRVLGPTGVGINAFTNSIVQYFILFGSLGINLYGNRGTAYRRDDRKALTTYF
WEVTILRFVTIGIAVLAYLMFIFAVDEYRVFYLAQGVMLLGTAFDISWFFQGLENFRVTVVRNVLVRIASLILIFLLVHK
ADDTALYILIMSGSQMLGNLTFWPSLRANLTHFPKLSSLNIWQHIKPAFLLLIPQLAIQIYVQLNKTMLGILQGVTASGF
YESSDKIIKMLLALVTATGTVLLPHVAHYFAQGDHDAVKRSLETSMHVILVIAFPLAFGIAAVSTTFTYYFFSTKFMPVA
PLMAAEAIVVIPISIASAIGVQYLLPTNQVKSYTVSVILGSIVNIVVNVPLILWLGTMGAVIGTILSESVVTIYQVYAIK
NQLDLRGLFSESWKYCLSAVVMFGVVKGLEIAWSTSLIGLVVEVLIGMVVYFVVLLGLRPHIIIGYVRPYVDQMRRRLR

Sequences:

>Translated_479_residues
MKVIKNFFWNAGYQVFVLIVPLVTVPYINRVLGPTGVGINAFTNSIVQYFILFGSLGINLYGNRGTAYRRDDRKALTTYF
WEVTILRFVTIGIAVLAYLMFIFAVDEYRVFYLAQGVMLLGTAFDISWFFQGLENFRVTVVRNVLVRIASLILIFLLVHK
ADDTALYILIMSGSQMLGNLTFWPSLRANLTHFPKLSSLNIWQHIKPAFLLLIPQLAIQIYVQLNKTMLGILQGVTASGF
YESSDKIIKMLLALVTATGTVLLPHVAHYFAQGDHDAVKRSLETSMHVILVIAFPLAFGIAAVSTTFTYYFFSTKFMPVA
PLMAAEAIVVIPISIASAIGVQYLLPTNQVKSYTVSVILGSIVNIVVNVPLILWLGTMGAVIGTILSESVVTIYQVYAIK
NQLDLRGLFSESWKYCLSAVVMFGVVKGLEIAWSTSLIGLVVEVLIGMVVYFVVLLGLRPHIIIGYVRPYVDQMRRRLR
>Mature_479_residues
MKVIKNFFWNAGYQVFVLIVPLVTVPYINRVLGPTGVGINAFTNSIVQYFILFGSLGINLYGNRGTAYRRDDRKALTTYF
WEVTILRFVTIGIAVLAYLMFIFAVDEYRVFYLAQGVMLLGTAFDISWFFQGLENFRVTVVRNVLVRIASLILIFLLVHK
ADDTALYILIMSGSQMLGNLTFWPSLRANLTHFPKLSSLNIWQHIKPAFLLLIPQLAIQIYVQLNKTMLGILQGVTASGF
YESSDKIIKMLLALVTATGTVLLPHVAHYFAQGDHDAVKRSLETSMHVILVIAFPLAFGIAAVSTTFTYYFFSTKFMPVA
PLMAAEAIVVIPISIASAIGVQYLLPTNQVKSYTVSVILGSIVNIVVNVPLILWLGTMGAVIGTILSESVVTIYQVYAIK
NQLDLRGLFSESWKYCLSAVVMFGVVKGLEIAWSTSLIGLVVEVLIGMVVYFVVLLGLRPHIIIGYVRPYVDQMRRRLR

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=396, Percent_Identity=23.989898989899, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 53473; Mature: 53473

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKNFFWNAGYQVFVLIVPLVTVPYINRVLGPTGVGINAFTNSIVQYFILFGSLGINL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
YGNRGTAYRRDDRKALTTYFWEVTILRFVTIGIAVLAYLMFIFAVDEYRVFYLAQGVMLL
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GTAFDISWFFQGLENFRVTVVRNVLVRIASLILIFLLVHKADDTALYILIMSGSQMLGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHCCE
TFWPSLRANLTHFPKLSSLNIWQHIKPAFLLLIPQLAIQIYVQLNKTMLGILQGVTASGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YESSDKIIKMLLALVTATGTVLLPHVAHYFAQGDHDAVKRSLETSMHVILVIAFPLAFGI
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVSTTFTYYFFSTKFMPVAPLMAAEAIVVIPISIASAIGVQYLLPTNQVKSYTVSVILG
HHHHHHHHHHEEECCCCCCHHHHHCCEEEEEEHHHHHHHCEEEECCCCHHHHHHHHHHHH
SIVNIVVNVPLILWLGTMGAVIGTILSESVVTIYQVYAIKNQLDLRGLFSESWKYCLSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VMFGVVKGLEIAWSTSLIGLVVEVLIGMVVYFVVLLGLRPHIIIGYVRPYVDQMRRRLR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVIKNFFWNAGYQVFVLIVPLVTVPYINRVLGPTGVGINAFTNSIVQYFILFGSLGINL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
YGNRGTAYRRDDRKALTTYFWEVTILRFVTIGIAVLAYLMFIFAVDEYRVFYLAQGVMLL
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
GTAFDISWFFQGLENFRVTVVRNVLVRIASLILIFLLVHKADDTALYILIMSGSQMLGNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHCCE
TFWPSLRANLTHFPKLSSLNIWQHIKPAFLLLIPQLAIQIYVQLNKTMLGILQGVTASGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YESSDKIIKMLLALVTATGTVLLPHVAHYFAQGDHDAVKRSLETSMHVILVIAFPLAFGI
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVSTTFTYYFFSTKFMPVAPLMAAEAIVVIPISIASAIGVQYLLPTNQVKSYTVSVILG
HHHHHHHHHHEEECCCCCCHHHHHCCEEEEEEHHHHHHHCEEEECCCCHHHHHHHHHHHH
SIVNIVVNVPLILWLGTMGAVIGTILSESVVTIYQVYAIKNQLDLRGLFSESWKYCLSAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VMFGVVKGLEIAWSTSLIGLVVEVLIGMVVYFVVLLGLRPHIIIGYVRPYVDQMRRRLR
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]