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The map label for this gene is ackA

Identifier: 116493738

GI number: 116493738

Start: 164876

End: 166066

Strand: Direct

Name: ackA

Synonym: LSEI_0166

Alternate gene names: 116493738

Gene position: 164876-166066 (Clockwise)

Preceding gene: 116493737

Following gene: 116493740

Centisome position: 5.69

GC content: 49.45

Gene sequence:

>1191_bases
ATGGCAAAAATTCTCGCAGTCAACGCAGGCAGTTCAACCCTGAAGTGGAAGCTTTTTGATATGCCAGCTGAGGTTCAGCT
GGCAGAAGGTTTGGTTGATCGGTTGGGACAGCCGCAATCAAAAGTTAAAATTAAGTATGGTGATGGGCAGAAATATGAAA
GCGATACGCCAATTGCTAACTATCAAGAAGCCGTCGCCAGTTTGATGGGTAACATCAAGGCGTTAGGGTTGGTTGAACAT
CTGCATGAGATTACTGGTGTGGGCCATCGTGTGGTAGCCGGCGGGGAGACTTTTGCCGAATCCGTTGTAGTTGATGATGC
TACCTTGTTGCAGATTCAAAACCTGCGTGACTACGCGCCTTTGCATAATCCGGTTGAAGCAGATTACATTGATGTTTTCA
AAAAGATGATGCCATGGGCCACAGAAGTCGCAGTGTTTGATACGGCGTTCCATCAAACCATGCAACCTGAGAACTTCCTC
TACAGTATTCCCTATGACTATTACAAGAAATATGGTGCTCGCAAATATGGTGCCCACGGGACAAGTGTGCGTTACGTCAG
CGCGCGGGCAGCTGAGATGCTTAACAAGCCGTTAGAAGACTTACGGATGATTGTGATGCATTTAGGCTCAGGCTCCAGTG
TTACCGCAGTCCAAGGCGGGCAGTCTATTGATACTTCCATGGGCTTTACGCCGTTAGCCGGGGTGACGATGGGGACACGA
TCCGGGGATATTGACCCATCACTTGTGGCTTATCTCATGAAGAAACTTGATGTCCCTGATGTGGGGCAAATGATTCATAT
CCTGAACAACGATTCCGGCCTGCTCGGTATTTCCGGCATCAGCAACGACATGCGGGATCTGGAAGCTGACGAAGATACCA
AGCCACGCGCCAAACTGGCATTGGATATTTTCGTTAACCGCGTTGTGAAGTACGTGGGTTCGTACGCAGCCTTGATGGAC
GGCGTGGATGTGCTTGTCTTCACAGCCGGGATCGGCGAAAACGGTGACGAGATTCGCGACAAGATCATGCGTTCACTCGA
TTACCTCGGCGCTAAGATCGACAATGACATCAACTACAAGTCACACGGCGTCGAAGCCGACCTCAGCACACCAGATTCAA
CTGTGAAAACATTGCTCGTGCCGACCAATGAAGAATTAATGATCGTCCGTGACGTCATGGCATTGAGCTAA

Upstream 100 bases:

>100_bases
ACACCCTGACCCCGATTCGCCGAAAACCAAGAAAAAGTCATGATGCTGTGCGATTACGTGTGCGGCAATCCGACTACCCG
TTTAGAGGAGGAAAAAAACA

Downstream 100 bases:

>100_bases
GCGTTAATGACTTTTCAATTAAAAAACCATCTCTCGACTGAAGGAGAGATGGTTTTTGTGTCTGCATAAGCTTCTGAGGA
ATGGACGAGCAAACAGACAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKILAVNAGSSTLKWKLFDMPAEVQLAEGLVDRLGQPQSKVKIKYGDGQKYESDTPIANYQEAVASLMGNIKALGLVEH
LHEITGVGHRVVAGGETFAESVVVDDATLLQIQNLRDYAPLHNPVEADYIDVFKKMMPWATEVAVFDTAFHQTMQPENFL
YSIPYDYYKKYGARKYGAHGTSVRYVSARAAEMLNKPLEDLRMIVMHLGSGSSVTAVQGGQSIDTSMGFTPLAGVTMGTR
SGDIDPSLVAYLMKKLDVPDVGQMIHILNNDSGLLGISGISNDMRDLEADEDTKPRAKLALDIFVNRVVKYVGSYAALMD
GVDVLVFTAGIGENGDEIRDKIMRSLDYLGAKIDNDINYKSHGVEADLSTPDSTVKTLLVPTNEELMIVRDVMALS

Sequences:

>Translated_396_residues
MAKILAVNAGSSTLKWKLFDMPAEVQLAEGLVDRLGQPQSKVKIKYGDGQKYESDTPIANYQEAVASLMGNIKALGLVEH
LHEITGVGHRVVAGGETFAESVVVDDATLLQIQNLRDYAPLHNPVEADYIDVFKKMMPWATEVAVFDTAFHQTMQPENFL
YSIPYDYYKKYGARKYGAHGTSVRYVSARAAEMLNKPLEDLRMIVMHLGSGSSVTAVQGGQSIDTSMGFTPLAGVTMGTR
SGDIDPSLVAYLMKKLDVPDVGQMIHILNNDSGLLGISGISNDMRDLEADEDTKPRAKLALDIFVNRVVKYVGSYAALMD
GVDVLVFTAGIGENGDEIRDKIMRSLDYLGAKIDNDINYKSHGVEADLSTPDSTVKTLLVPTNEELMIVRDVMALS
>Mature_395_residues
AKILAVNAGSSTLKWKLFDMPAEVQLAEGLVDRLGQPQSKVKIKYGDGQKYESDTPIANYQEAVASLMGNIKALGLVEHL
HEITGVGHRVVAGGETFAESVVVDDATLLQIQNLRDYAPLHNPVEADYIDVFKKMMPWATEVAVFDTAFHQTMQPENFLY
SIPYDYYKKYGARKYGAHGTSVRYVSARAAEMLNKPLEDLRMIVMHLGSGSSVTAVQGGQSIDTSMGFTPLAGVTMGTRS
GDIDPSLVAYLMKKLDVPDVGQMIHILNNDSGLLGISGISNDMRDLEADEDTKPRAKLALDIFVNRVVKYVGSYAALMDG
VDVLVFTAGIGENGDEIRDKIMRSLDYLGAKIDNDINYKSHGVEADLSTPDSTVKTLLVPTNEELMIVRDVMALS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=41.5617128463476, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=38.3838383838384, Blast_Score=289, Evalue=2e-79,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_LACC3 (Q03CP2)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805472.1
- ProteinModelPortal:   Q03CP2
- SMR:   Q03CP2
- STRING:   Q03CP2
- GeneID:   4419118
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0166
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   WATEVAV
- PhylomeDB:   Q03CP2
- ProtClustDB:   PRK00180
- BioCyc:   LCAS321967:LSEI_0166-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43262; Mature: 43131

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILAVNAGSSTLKWKLFDMPAEVQLAEGLVDRLGQPQSKVKIKYGDGQKYESDTPIAN
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHH
YQEAVASLMGNIKALGLVEHLHEITGVGHRVVAGGETFAESVVVDDATLLQIQNLRDYAP
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHEECCHHHHHHHHHHHHCC
LHNPVEADYIDVFKKMMPWATEVAVFDTAFHQTMQPENFLYSIPYDYYKKYGARKYGAHG
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCC
TSVRYVSARAAEMLNKPLEDLRMIVMHLGSGSSVTAVQGGQSIDTSMGFTPLAGVTMGTR
CEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCC
SGDIDPSLVAYLMKKLDVPDVGQMIHILNNDSGLLGISGISNDMRDLEADEDTKPRAKLA
CCCCCHHHHHHHHHHCCCCCHHHEEEEEECCCCEEEECCCCCHHHHCCCCCCCCCHHHHH
LDIFVNRVVKYVGSYAALMDGVDVLVFTAGIGENGDEIRDKIMRSLDYLGAKIDNDINYK
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SHGVEADLSTPDSTVKTLLVPTNEELMIVRDVMALS
CCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHCC
>Mature Secondary Structure 
AKILAVNAGSSTLKWKLFDMPAEVQLAEGLVDRLGQPQSKVKIKYGDGQKYESDTPIAN
CEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHH
YQEAVASLMGNIKALGLVEHLHEITGVGHRVVAGGETFAESVVVDDATLLQIQNLRDYAP
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHEECCHHHHHHHHHHHHCC
LHNPVEADYIDVFKKMMPWATEVAVFDTAFHQTMQPENFLYSIPYDYYKKYGARKYGAHG
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCCC
TSVRYVSARAAEMLNKPLEDLRMIVMHLGSGSSVTAVQGGQSIDTSMGFTPLAGVTMGTR
CEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCC
SGDIDPSLVAYLMKKLDVPDVGQMIHILNNDSGLLGISGISNDMRDLEADEDTKPRAKLA
CCCCCHHHHHHHHHHCCCCCHHHEEEEEECCCCEEEECCCCCHHHHCCCCCCCCCHHHHH
LDIFVNRVVKYVGSYAALMDGVDVLVFTAGIGENGDEIRDKIMRSLDYLGAKIDNDINYK
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SHGVEADLSTPDSTVKTLLVPTNEELMIVRDVMALS
CCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA