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The map label for this gene is purA

Identifier: 116493694

GI number: 116493694

Start: 118095

End: 119390

Strand: Direct

Name: purA

Synonym: LSEI_0122

Alternate gene names: 116493694

Gene position: 118095-119390 (Clockwise)

Preceding gene: 116493690

Following gene: 116493695

Centisome position: 4.08

GC content: 49.23

Gene sequence:

>1296_bases
ATGGGAACTGTAGTTATTGTCGGTACACAATGGGGCGACGAAGGTAAAGGGAAGATTACGGATTTCCTGAGCCAAGGCGC
CAAGGTGGTCAGCCGTTATCAAGGCGGTGACAATGCAGGCCATACGATCCATGCAAATGGCGAGGTTTATAAGCTGCGCC
TCGTACCATCAGGGGTACTCTACCCGCATCAGTTATCGGTGATCGGTAATGGTGTTGTTGTGAATCCTAAGAGCTTGGTC
GGTGAATTGGCCCGGCTCGCAGAACAAGGCGTGACTGGCGAAAATCTGCGGATTTCAGACCGTGCTCACGTGATTTTGCC
TTATCATATTAAATTAGATAAATTGCAGGAAGCAGCCAAGGGCGCCGACAAGATTGGGACGACCAATCGCGGCATCGGCC
CGGCCTATATGGACAAGGCTGCTCGAGTTGGCATTCGGATGGCTGACTTGCTCGACAAAGAAATCTTTGAAGAACGCCTC
AAAGCTAATTTGAAAGCTAAGAATGAAGAATTCGTCAAAGTTTATGATAGTACGCCGATGAATTTTGATGATATCTTTGA
AGAATATTATCAATATGGCCAACAATTGAAACAGTATGTTTGCGACACCTCAATTGTGTTGAACGATGCGATTGACAAAG
GTGAACACGTTTTGTTTGAAGGCGCGCAAGGGATTATGCTGGATATTGACCAAGGGACCTATCCGTTCGTAACGTCATCG
AACCCTGCTGGCGGTGTGACGGTTGGTGCCGGCGTTGGTGCCTCCAAGATTGATCGGGTTGTGGGGGTTGCGAAAGCTTA
TACCTCCCGCGTTGGCGACGGTCCGTTCCCAACTGAATTGCTCGATAAAACCGGAGATTTCATTCGCAATGCTGGCCACG
AATTCGGTACTGTGACCGGCCGACCTCGGCGGATTGGCTGGTTCGATGCTGTCGTTGTTCGTCATTCTCGCCGTGTTGCC
GGGATTACGGATCTGTGCCTCAACTCAATTGATGTGCTAACCGGTTTGGACAAAGTCAAAATCTGTGTTGCCTACGAACG
CGATGGCGAACGGGTTGAAAACTACCCAGCTTCGCTCAAGTTCCTCAGTGAATGCAAGCCAGTGTATGAAGAACTGCCAG
GTTGGCAAGAAGATATTACCAAAGCCAAAACTTTGGATGATCTTCCGGAAAATGCGCGGCGTTACGTTGAACGCATCACA
GAATTACTTGGTGTTGACCTGCTGACATTCTCAGTTGGCCCTGACCGTGACCAAACCAATGTCCTTGAGAATGTTTGGGA
TAAGGTATCGCGCTAG

Upstream 100 bases:

>100_bases
TTTAAAGTTCGGATTTTGCTTGTTTTCATACCGCTATCCTGTTAGACTACCAGATGGCGGGTAAAAATGCCCGCACTAAG
CCGTGAGGAGCGATCAGTTA

Downstream 100 bases:

>100_bases
ACGCACCGCGATTTGATAATTCAATAGCGATTTTTTAAGAGCTGCTAGGTGAAAATTCACTGGTGGCTCTTTTTTTGACA
AATTAGGACATCATTCATTG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGTVVIVGTQWGDEGKGKITDFLSQGAKVVSRYQGGDNAGHTIHANGEVYKLRLVPSGVLYPHQLSVIGNGVVVNPKSLV
GELARLAEQGVTGENLRISDRAHVILPYHIKLDKLQEAAKGADKIGTTNRGIGPAYMDKAARVGIRMADLLDKEIFEERL
KANLKAKNEEFVKVYDSTPMNFDDIFEEYYQYGQQLKQYVCDTSIVLNDAIDKGEHVLFEGAQGIMLDIDQGTYPFVTSS
NPAGGVTVGAGVGASKIDRVVGVAKAYTSRVGDGPFPTELLDKTGDFIRNAGHEFGTVTGRPRRIGWFDAVVVRHSRRVA
GITDLCLNSIDVLTGLDKVKICVAYERDGERVENYPASLKFLSECKPVYEELPGWQEDITKAKTLDDLPENARRYVERIT
ELLGVDLLTFSVGPDRDQTNVLENVWDKVSR

Sequences:

>Translated_431_residues
MGTVVIVGTQWGDEGKGKITDFLSQGAKVVSRYQGGDNAGHTIHANGEVYKLRLVPSGVLYPHQLSVIGNGVVVNPKSLV
GELARLAEQGVTGENLRISDRAHVILPYHIKLDKLQEAAKGADKIGTTNRGIGPAYMDKAARVGIRMADLLDKEIFEERL
KANLKAKNEEFVKVYDSTPMNFDDIFEEYYQYGQQLKQYVCDTSIVLNDAIDKGEHVLFEGAQGIMLDIDQGTYPFVTSS
NPAGGVTVGAGVGASKIDRVVGVAKAYTSRVGDGPFPTELLDKTGDFIRNAGHEFGTVTGRPRRIGWFDAVVVRHSRRVA
GITDLCLNSIDVLTGLDKVKICVAYERDGERVENYPASLKFLSECKPVYEELPGWQEDITKAKTLDDLPENARRYVERIT
ELLGVDLLTFSVGPDRDQTNVLENVWDKVSR
>Mature_430_residues
GTVVIVGTQWGDEGKGKITDFLSQGAKVVSRYQGGDNAGHTIHANGEVYKLRLVPSGVLYPHQLSVIGNGVVVNPKSLVG
ELARLAEQGVTGENLRISDRAHVILPYHIKLDKLQEAAKGADKIGTTNRGIGPAYMDKAARVGIRMADLLDKEIFEERLK
ANLKAKNEEFVKVYDSTPMNFDDIFEEYYQYGQQLKQYVCDTSIVLNDAIDKGEHVLFEGAQGIMLDIDQGTYPFVTSSN
PAGGVTVGAGVGASKIDRVVGVAKAYTSRVGDGPFPTELLDKTGDFIRNAGHEFGTVTGRPRRIGWFDAVVVRHSRRVAG
ITDLCLNSIDVLTGLDKVKICVAYERDGERVENYPASLKFLSECKPVYEELPGWQEDITKAKTLDDLPENARRYVERITE
LLGVDLLTFSVGPDRDQTNVLENVWDKVSR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=421, Percent_Identity=40.1425178147268, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI34577063, Length=423, Percent_Identity=39.7163120567376, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI40316944, Length=392, Percent_Identity=38.7755102040816, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=47.7751756440281, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25146553, Length=422, Percent_Identity=40.9952606635071, Blast_Score=314, Evalue=7e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=422, Percent_Identity=40.9952606635071, Blast_Score=313, Evalue=8e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=427, Percent_Identity=40.0468384074941, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI21356033, Length=419, Percent_Identity=42.7207637231504, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_LACC3 (Q03CT6)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805428.1
- ProteinModelPortal:   Q03CT6
- SMR:   Q03CT6
- STRING:   Q03CT6
- GeneID:   4419011
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0122
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q03CT6
- ProtClustDB:   PRK01117
- BioCyc:   LCAS321967:LSEI_0122-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47392; Mature: 47261

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 301-301 BINDING 303-303

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTVVIVGTQWGDEGKGKITDFLSQGAKVVSRYQGGDNAGHTIHANGEVYKLRLVPSGVL
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCCCC
YPHQLSVIGNGVVVNPKSLVGELARLAEQGVTGENLRISDRAHVILPYHIKLDKLQEAAK
CCCEEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEHHHHHHHHC
GADKIGTTNRGIGPAYMDKAARVGIRMADLLDKEIFEERLKANLKAKNEEFVKVYDSTPM
CHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
NFDDIFEEYYQYGQQLKQYVCDTSIVLNDAIDKGEHVLFEGAQGIMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCCEEEEECCCEEEEEECCCCCCEEECC
NPAGGVTVGAGVGASKIDRVVGVAKAYTSRVGDGPFPTELLDKTGDFIRNAGHEFGTVTG
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
RPRRIGWFDAVVVRHSRRVAGITDLCLNSIDVLTGLDKVKICVAYERDGERVENYPASLK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCHHHH
FLSECKPVYEELPGWQEDITKAKTLDDLPENARRYVERITELLGVDLLTFSVGPDRDQTN
HHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHH
VLENVWDKVSR
HHHHHHHHHCC
>Mature Secondary Structure 
GTVVIVGTQWGDEGKGKITDFLSQGAKVVSRYQGGDNAGHTIHANGEVYKLRLVPSGVL
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCEEEEEECCCCCC
YPHQLSVIGNGVVVNPKSLVGELARLAEQGVTGENLRISDRAHVILPYHIKLDKLQEAAK
CCCEEEEECCCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEHHHHHHHHC
GADKIGTTNRGIGPAYMDKAARVGIRMADLLDKEIFEERLKANLKAKNEEFVKVYDSTPM
CHHHCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
NFDDIFEEYYQYGQQLKQYVCDTSIVLNDAIDKGEHVLFEGAQGIMLDIDQGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCCCEEEEECCCEEEEEECCCCCCEEECC
NPAGGVTVGAGVGASKIDRVVGVAKAYTSRVGDGPFPTELLDKTGDFIRNAGHEFGTVTG
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
RPRRIGWFDAVVVRHSRRVAGITDLCLNSIDVLTGLDKVKICVAYERDGERVENYPASLK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHCCCHHHH
FLSECKPVYEELPGWQEDITKAKTLDDLPENARRYVERITELLGVDLLTFSVGPDRDQTN
HHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHH
VLENVWDKVSR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA