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The map label for this gene is ygcA [C]

Identifier: 116493460

GI number: 116493460

Start: 1708637

End: 1710112

Strand: Direct

Name: ygcA [C]

Synonym: PEPE_1732

Alternate gene names: 116493460

Gene position: 1708637-1710112 (Clockwise)

Preceding gene: 116493457

Following gene: 116493465

Centisome position: 93.25

GC content: 39.09

Gene sequence:

>1476_bases
ATGAATAACAAAGAAAAAACAAACTATCAAGGCAAAAAAAAATTCGGTAAAAAACCTAGTGACCGTCAAAAACAAGACCG
TCGGAAGAATTTTTACAATAATCCCCGTAAATCTTCTGACATCAAGATTGGTGACGAAATTCCACTTAAAATTAAACGTT
TGGGTATAAACGGTGAAGGAATTGGTTACTACCAGCGTACTATCTGTTTCATCAAAGGTGCGCTTCCTGGTGAAAAGGTC
GTAGCATCCATTACCGATGTTCATCCCCGTTTTATCAATGGTGAAGTAAGTAAAATCAAAAAAATCAGTCCCGATCGGGT
AAAACCCATTGACGCATATGCTGGAGAAGTCGGTGGATTCGAACTTGAACATTTAGCCTATCCTGCTCAACTTGAATTTA
AGCGTGATGTTATTCGACAATCACTAACTAAATTCAGACCCGAAGGCTACAAACGTTTCGAACTTCTACCAACCATCGGA
ATGGAAAAACCCCTTGAATATCGTAATAAGGCTCAATTCCAAGTCCGTCGTTTAGATGGTAAGGTAGCGGCTGGACTCTA
TAAGGAAGGTACTCATGATCTAGTAGATTTACCAACTTGTAGCGTTCAACACCCTTTAACGATGGAAATCATTCGAATTG
CGGTTGAATTATTCGAAAAATACAACGTAAGCATCTATGATGAACAAACTCATACGGGAAGCCTAAAAACGATCGTAGTA
CGTGTTGCTGACGCAACTAAAGAAACGCAAGTAGTCTTTATTACTAATACGCCAGAATTAGAACATGTAGACGAAATTAT
TGATGAATTAGCAGCTCAATTCCCTGAAATTGTTTCCTTCATGCAAAATCTTAACCCTAGTCGCGAATCATTAATCTGGG
GTGATAAGACTTTAAACCTGCGTGGTAAGGATACCATTCGAGAAGAATTGGATGGTCTATCATTCAACCTATCCGCACGC
GCCTTCTTCCAACTTAATCCTAAGCAAACTTCTGTACTTTATGCTGAAACTCGTAAAGCCCTCGAGTTGTCTCCTGATGA
CAATCTTGTTGATGCTTACTCTGGAGTTGGAACCATTGGACTTTCACTTGCTGCTCAAGCTAAGGAAGTCCGTGGAATGG
ATAACATTGAATCAGCGATTGAAGATGCTAATGAAAATGCAAAAATTAATGGCATCGAAAACGCTCACTATGAATTTGGT
ACAGCTGAGAAAGTTCTTCCAAAATGGCTACATGCCGGTTTTAAACCGAACTCAATTGTGGTCGATCCACCTCGCGTTGG
TTTAGATAATAAATTAATCACCGCAATTTTGGAATCTGCACCTGAACATTTCACATATGTTTCATGTAATCCCTCCACTT
TAGCTCGTGATTTAGTTCGTATCGTTGAAAAATACGATGTCGAATACATTCAATCTATCGATATGTTCCCTCAAACAGCT
CGTGTGGAAGCAGTAGTTAAGTTAAAACGTAAATAA

Upstream 100 bases:

>100_bases
CAAATTCAAGTGTAACGAAAATTAGTTCAAAATCACACATCAAATTTTGGTATCCTTAATATGTTAGAATTGTTAGTTGA
CACATAAAATCGAGGTAATC

Downstream 100 bases:

>100_bases
AAAGTTGTGACAAAAGGCTGAGTTTAAGCGTATAGCTATGAGAACTCAAACTACCTTTTGTCGCAACTTTTTTAATTTAT
CCATTTATTTTTCTTAGCAA

Product: tRNA (uracil-5-)-methyltransferase related enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MNNKEKTNYQGKKKFGKKPSDRQKQDRRKNFYNNPRKSSDIKIGDEIPLKIKRLGINGEGIGYYQRTICFIKGALPGEKV
VASITDVHPRFINGEVSKIKKISPDRVKPIDAYAGEVGGFELEHLAYPAQLEFKRDVIRQSLTKFRPEGYKRFELLPTIG
MEKPLEYRNKAQFQVRRLDGKVAAGLYKEGTHDLVDLPTCSVQHPLTMEIIRIAVELFEKYNVSIYDEQTHTGSLKTIVV
RVADATKETQVVFITNTPELEHVDEIIDELAAQFPEIVSFMQNLNPSRESLIWGDKTLNLRGKDTIREELDGLSFNLSAR
AFFQLNPKQTSVLYAETRKALELSPDDNLVDAYSGVGTIGLSLAAQAKEVRGMDNIESAIEDANENAKINGIENAHYEFG
TAEKVLPKWLHAGFKPNSIVVDPPRVGLDNKLITAILESAPEHFTYVSCNPSTLARDLVRIVEKYDVEYIQSIDMFPQTA
RVEAVVKLKRK

Sequences:

>Translated_491_residues
MNNKEKTNYQGKKKFGKKPSDRQKQDRRKNFYNNPRKSSDIKIGDEIPLKIKRLGINGEGIGYYQRTICFIKGALPGEKV
VASITDVHPRFINGEVSKIKKISPDRVKPIDAYAGEVGGFELEHLAYPAQLEFKRDVIRQSLTKFRPEGYKRFELLPTIG
MEKPLEYRNKAQFQVRRLDGKVAAGLYKEGTHDLVDLPTCSVQHPLTMEIIRIAVELFEKYNVSIYDEQTHTGSLKTIVV
RVADATKETQVVFITNTPELEHVDEIIDELAAQFPEIVSFMQNLNPSRESLIWGDKTLNLRGKDTIREELDGLSFNLSAR
AFFQLNPKQTSVLYAETRKALELSPDDNLVDAYSGVGTIGLSLAAQAKEVRGMDNIESAIEDANENAKINGIENAHYEFG
TAEKVLPKWLHAGFKPNSIVVDPPRVGLDNKLITAILESAPEHFTYVSCNPSTLARDLVRIVEKYDVEYIQSIDMFPQTA
RVEAVVKLKRK
>Mature_491_residues
MNNKEKTNYQGKKKFGKKPSDRQKQDRRKNFYNNPRKSSDIKIGDEIPLKIKRLGINGEGIGYYQRTICFIKGALPGEKV
VASITDVHPRFINGEVSKIKKISPDRVKPIDAYAGEVGGFELEHLAYPAQLEFKRDVIRQSLTKFRPEGYKRFELLPTIG
MEKPLEYRNKAQFQVRRLDGKVAAGLYKEGTHDLVDLPTCSVQHPLTMEIIRIAVELFEKYNVSIYDEQTHTGSLKTIVV
RVADATKETQVVFITNTPELEHVDEIIDELAAQFPEIVSFMQNLNPSRESLIWGDKTLNLRGKDTIREELDGLSFNLSAR
AFFQLNPKQTSVLYAETRKALELSPDDNLVDAYSGVGTIGLSLAAQAKEVRGMDNIESAIEDANENAKINGIENAHYEFG
TAEKVLPKWLHAGFKPNSIVVDPPRVGLDNKLITAILESAPEHFTYVSCNPSTLARDLVRIVEKYDVEYIQSIDMFPQTA
RVEAVVKLKRK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=363, Percent_Identity=29.4765840220386, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI51173878, Length=363, Percent_Identity=29.4765840220386, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI269784680, Length=208, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI269784676, Length=208, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI20149688, Length=208, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI269784678, Length=206, Percent_Identity=33.9805825242718, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1789148, Length=473, Percent_Identity=25.1585623678647, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787083, Length=374, Percent_Identity=25.4010695187166, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790403, Length=156, Percent_Identity=29.4871794871795, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71997438, Length=169, Percent_Identity=32.5443786982249, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=446, Percent_Identity=24.4394618834081, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24666579, Length=424, Percent_Identity=27.8301886792453, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 55485; Mature: 55485

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKEKTNYQGKKKFGKKPSDRQKQDRRKNFYNNPRKSSDIKIGDEIPLKIKRLGINGEG
CCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCC
IGYYQRTICFIKGALPGEKVVASITDVHPRFINGEVSKIKKISPDRVKPIDAYAGEVGGF
CCHHHHEEEEEECCCCHHHHHHHHHHCCCHHCCCHHHHHHCCCCCCCCCHHHHCCCCCCE
ELEHLAYPAQLEFKRDVIRQSLTKFRPEGYKRFELLPTIGMEKPLEYRNKAQFQVRRLDG
EHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCHHEEEEECCC
KVAAGLYKEGTHDLVDLPTCSVQHPLTMEIIRIAVELFEKYNVSIYDEQTHTGSLKTIVV
HHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
RVADATKETQVVFITNTPELEHVDEIIDELAAQFPEIVSFMQNLNPSRESLIWGDKTLNL
EEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCEEECC
RGKDTIREELDGLSFNLSARAFFQLNPKQTSVLYAETRKALELSPDDNLVDAYSGVGTIG
CCCHHHHHHHCCCEEEECEEEEEEECCCHHEEEEECCHHHEECCCCCCHHHHHCCCHHHH
LSLAAQAKEVRGMDNIESAIEDANENAKINGIENAHYEFGTAEKVLPKWLHAGFKPNSIV
HHHHHHHHHHCCCHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
VDPPRVGLDNKLITAILESAPEHFTYVSCNPSTLARDLVRIVEKYDVEYIQSIDMFPQTA
ECCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHH
RVEAVVKLKRK
HHHHHHHHCCC
>Mature Secondary Structure
MNNKEKTNYQGKKKFGKKPSDRQKQDRRKNFYNNPRKSSDIKIGDEIPLKIKRLGINGEG
CCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCC
IGYYQRTICFIKGALPGEKVVASITDVHPRFINGEVSKIKKISPDRVKPIDAYAGEVGGF
CCHHHHEEEEEECCCCHHHHHHHHHHCCCHHCCCHHHHHHCCCCCCCCCHHHHCCCCCCE
ELEHLAYPAQLEFKRDVIRQSLTKFRPEGYKRFELLPTIGMEKPLEYRNKAQFQVRRLDG
EHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHCCHHEEEEECCC
KVAAGLYKEGTHDLVDLPTCSVQHPLTMEIIRIAVELFEKYNVSIYDEQTHTGSLKTIVV
HHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEE
RVADATKETQVVFITNTPELEHVDEIIDELAAQFPEIVSFMQNLNPSRESLIWGDKTLNL
EEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCEEECC
RGKDTIREELDGLSFNLSARAFFQLNPKQTSVLYAETRKALELSPDDNLVDAYSGVGTIG
CCCHHHHHHHCCCEEEECEEEEEEECCCHHEEEEECCHHHEECCCCCCHHHHHCCCHHHH
LSLAAQAKEVRGMDNIESAIEDANENAKINGIENAHYEFGTAEKVLPKWLHAGFKPNSIV
HHHHHHHHHHCCCHHHHHHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
VDPPRVGLDNKLITAILESAPEHFTYVSCNPSTLARDLVRIVEKYDVEYIQSIDMFPQTA
ECCCCCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHHHHCCCCHH
RVEAVVKLKRK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]