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The map label for this gene is manX [H]

Identifier: 116493251

GI number: 116493251

Start: 1479748

End: 1480248

Strand: Reverse

Name: manX [H]

Synonym: PEPE_1513

Alternate gene names: 116493251

Gene position: 1480248-1479748 (Counterclockwise)

Preceding gene: 116493252

Following gene: 116493249

Centisome position: 80.78

GC content: 38.92

Gene sequence:

>501_bases
ATGACGATGGACATTCTACTGGCTCGTGTCGATAGTCGGCTCTTGCATGGGCAAGTAGCGACTGCTTGGGCTAAAAAGGT
TAAACCTAATCGGATTTTAGTCGTTTCAGATAGCGTTGCAGAAGATAATTTACGTAAAACGTTGATTACTCAAGCTGCGC
CTCCTGGAATATTAGTAAACGTGATTACGGTTGAAAAAATGATTCGAATTTATCAAGATTCGCAATTTGATTCTTTTAAA
GTGTTACTTTTAACGGAAACCCTACAAGATATGTTGAAGCTGGTTGATGGCGGTATCGATGTCTCTGATTTAGGGATTGA
TGTGGGCAGTTTGGCATACGCCGTAGATATGACGATGCTAACAGATGCGATTGCGGTTGGTAAAGAAGAGATTGCTACAA
TCCGAACCCTACACGAACGTGGATTAACAGTGTATGCTCAGAAAATTCCTAATGATAGCCGTAAAGATTTGATAAAAATG
TTAGATAAAAATAATTTATAA

Upstream 100 bases:

>100_bases
TTTTATGTTTATAAATTGTTGGAAACATGGCAAGAAAAAAATGATACAATCCTAAGTGAGGTAGATTAAAACTAATCTTA
ATTTTGGAGGACACGGTGAT

Downstream 100 bases:

>100_bases
TAAAAAATAACCCACAACTTTAATAGTTATGGGTTATTTTTATGGTCCAAATAAATGTTAAAGTTCCTCAAGTACCTGAT
TTTCTTTAATCTTTGGTTCG

Product: phosphotransferase system, mannose/fructose-specific component IIB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTMDILLARVDSRLLHGQVATAWAKKVKPNRILVVSDSVAEDNLRKTLITQAAPPGILVNVITVEKMIRIYQDSQFDSFK
VLLLTETLQDMLKLVDGGIDVSDLGIDVGSLAYAVDMTMLTDAIAVGKEEIATIRTLHERGLTVYAQKIPNDSRKDLIKM
LDKNNL

Sequences:

>Translated_166_residues
MTMDILLARVDSRLLHGQVATAWAKKVKPNRILVVSDSVAEDNLRKTLITQAAPPGILVNVITVEKMIRIYQDSQFDSFK
VLLLTETLQDMLKLVDGGIDVSDLGIDVGSLAYAVDMTMLTDAIAVGKEEIATIRTLHERGLTVYAQKIPNDSRKDLIKM
LDKNNL
>Mature_165_residues
TMDILLARVDSRLLHGQVATAWAKKVKPNRILVVSDSVAEDNLRKTLITQAAPPGILVNVITVEKMIRIYQDSQFDSFKV
LLLTETLQDMLKLVDGGIDVSDLGIDVGSLAYAVDMTMLTDAIAVGKEEIATIRTLHERGLTVYAQKIPNDSRKDLIKML
DKNNL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=164, Percent_Identity=40.8536585365854, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI87082217, Length=159, Percent_Identity=34.5911949685535, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789527, Length=155, Percent_Identity=30.9677419354839, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18377; Mature: 18246

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDILLARVDSRLLHGQVATAWAKKVKPNRILVVSDSVAEDNLRKTLITQAAPPGILVN
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEH
VITVEKMIRIYQDSQFDSFKVLLLTETLQDMLKLVDGGIDVSDLGIDVGSLAYAVDMTML
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHH
TDAIAVGKEEIATIRTLHERGLTVYAQKIPNDSRKDLIKMLDKNNL
HHHHHHCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TMDILLARVDSRLLHGQVATAWAKKVKPNRILVVSDSVAEDNLRKTLITQAAPPGILVN
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEH
VITVEKMIRIYQDSQFDSFKVLLLTETLQDMLKLVDGGIDVSDLGIDVGSLAYAVDMTML
HHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHH
TDAIAVGKEEIATIRTLHERGLTVYAQKIPNDSRKDLIKMLDKNNL
HHHHHHCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA