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The map label for this gene is gltX

Identifier: 116493239

GI number: 116493239

Start: 1468024

End: 1469511

Strand: Reverse

Name: gltX

Synonym: PEPE_1501

Alternate gene names: 116493239

Gene position: 1469511-1468024 (Counterclockwise)

Preceding gene: 116493240

Following gene: 116493238

Centisome position: 80.2

GC content: 37.5

Gene sequence:

>1488_bases
TTGGCAAAGCAAAAGATTCGAGTACGTTATGCTCCAAGTCCAACGGGACATCTGCATATCGGAAATGCACGAACAGCCTT
ATTTAATTATTTGTTTGCTCGTCATTACAAAGGTAAATTTATTCTTAGAATTGAAGATACTGACCAAAGTCGGAATATCG
CTGACGGAGAAAAAAGTCAGATCGATAACCTAAAATGGTTGGGAATGGATTGGGATGAAGGTCCTGATAAACCGGGAGAA
TACGGTCCATATCGTCAATCTGAACGTAAAGGTATATATCAACCACTAATTGATCAGTTAGTTGCTGAAGGTAAAGCATA
TGAATCATATATGACTGAAGAAGAACTAGCTGCTCAACGTGAAGAGCAAAAGGCAAATGGTGAAATGCCTCATTATGTTG
ATGAATATGCTGGATTATCACCTGAACAAAAAGAACAAAAAATTGCTGATGCTAAGGCAGCAGGAATTGAACCTGTAATT
CGTTTTAAGGTGCCTACCAACACTACTTACGAATGGGATGATTTGGTTAAGGGTCATATTTCATTTGAATCTGAAACAAT
TGGTGGCGATTTTGTCATTCAAAAGCGTGATGGAATGCCAACTTACAACTTTGCGGTAGTTGTTGATGATCACATGATGG
AAATTAGCCATGTTTTCCGTGGGGATGACCACGTTGCAAATACTCCTAAACAATTGATGATTTATGAAGCTTTAGGATGG
GATGCTCCCCAATTTGGTCACATGAGCTTAATTATCAATACTGAAACAGGTAAGAAGTTAAGTAAACGTGATGAATCTAT
TCTTCAATTCATCGAACAATATCGTAGTTTAGGATATCTACCAGAAGCAATGTTGAACTTTATTATCTTGCTCGGCTGGT
CACCAGTGGGAGAAAGTGAAATCTTTACTCTACGTGAATTCGTTAAGATGTATGATGAAAAGCGTTTGAGTAAGTCACCT
GCTGCTTTTGATGCTAAGAAATTGGAATGGATTAACAACCAATATGTTAAAGCTGCTGATGAAGATCGTATCATGCACTC
AGCACTCCTTCAATTGATTAATGCCGGTAAGATTGAAAAGAACCCAGCACCAATGAAGGTAGAATGGGCACGTAAGTTAA
TCAACTTATTTAAGCGTCAAATGAGTTATACAGCGCAAATTGTTGAATTTACAGAATTATTCTTTAATGGTCCTGAAAAT
ATCGATGAAGAGGCTAAAGAAGAATTGGCCACGGATGATGCTCTTCCAGTATTAAAAGCATTGGAAAAACAATTCAGTGA
TTTGCCAGTCTTTGATACAGTTAACATTTTGGCAGCTATTAAGGCGGTTCAAAAGGAAACTGGAATTAAGGGTCGCAAAC
TTTGGATGCCAATTCGAATTGCTATTACCCATGAAATGCATGGACCAGAACTTCCAGAAAGTTTGGAATTATTAGGTTAT
GAATTATCAATGCAACATTTACGGGCAATGATTGAAGAATTAAGCTAA

Upstream 100 bases:

>100_bases
AATTACCCATAGCAAAGATAAGACAGAATAGACTTTATTTATTATTGATATCAATGTAGAATGAAAACATTGTGACATAA
TTTTTGGAGAGGTGTTAATT

Downstream 100 bases:

>100_bases
ATATGAAAGAAGAACTTCAATAAAGTTAGGTGGGAGACATGGACTCCGCTACCAAACGTTGATGCTTGGCTCAGCGACTA
AAACTTTTTTGAAGCTCTTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MAKQKIRVRYAPSPTGHLHIGNARTALFNYLFARHYKGKFILRIEDTDQSRNIADGEKSQIDNLKWLGMDWDEGPDKPGE
YGPYRQSERKGIYQPLIDQLVAEGKAYESYMTEEELAAQREEQKANGEMPHYVDEYAGLSPEQKEQKIADAKAAGIEPVI
RFKVPTNTTYEWDDLVKGHISFESETIGGDFVIQKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEALGW
DAPQFGHMSLIINTETGKKLSKRDESILQFIEQYRSLGYLPEAMLNFIILLGWSPVGESEIFTLREFVKMYDEKRLSKSP
AAFDAKKLEWINNQYVKAADEDRIMHSALLQLINAGKIEKNPAPMKVEWARKLINLFKRQMSYTAQIVEFTELFFNGPEN
IDEEAKEELATDDALPVLKALEKQFSDLPVFDTVNILAAIKAVQKETGIKGRKLWMPIRIAITHEMHGPELPESLELLGY
ELSMQHLRAMIEELS

Sequences:

>Translated_495_residues
MAKQKIRVRYAPSPTGHLHIGNARTALFNYLFARHYKGKFILRIEDTDQSRNIADGEKSQIDNLKWLGMDWDEGPDKPGE
YGPYRQSERKGIYQPLIDQLVAEGKAYESYMTEEELAAQREEQKANGEMPHYVDEYAGLSPEQKEQKIADAKAAGIEPVI
RFKVPTNTTYEWDDLVKGHISFESETIGGDFVIQKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEALGW
DAPQFGHMSLIINTETGKKLSKRDESILQFIEQYRSLGYLPEAMLNFIILLGWSPVGESEIFTLREFVKMYDEKRLSKSP
AAFDAKKLEWINNQYVKAADEDRIMHSALLQLINAGKIEKNPAPMKVEWARKLINLFKRQMSYTAQIVEFTELFFNGPEN
IDEEAKEELATDDALPVLKALEKQFSDLPVFDTVNILAAIKAVQKETGIKGRKLWMPIRIAITHEMHGPELPESLELLGY
ELSMQHLRAMIEELS
>Mature_494_residues
AKQKIRVRYAPSPTGHLHIGNARTALFNYLFARHYKGKFILRIEDTDQSRNIADGEKSQIDNLKWLGMDWDEGPDKPGEY
GPYRQSERKGIYQPLIDQLVAEGKAYESYMTEEELAAQREEQKANGEMPHYVDEYAGLSPEQKEQKIADAKAAGIEPVIR
FKVPTNTTYEWDDLVKGHISFESETIGGDFVIQKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEALGWD
APQFGHMSLIINTETGKKLSKRDESILQFIEQYRSLGYLPEAMLNFIILLGWSPVGESEIFTLREFVKMYDEKRLSKSPA
AFDAKKLEWINNQYVKAADEDRIMHSALLQLINAGKIEKNPAPMKVEWARKLINLFKRQMSYTAQIVEFTELFFNGPENI
DEEAKEELATDDALPVLKALEKQFSDLPVFDTVNILAAIKAVQKETGIKGRKLWMPIRIAITHEMHGPELPESLELLGYE
LSMQHLRAMIEELS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=334, Percent_Identity=39.8203592814371, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI62241042, Length=260, Percent_Identity=23.4615384615385, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788743, Length=489, Percent_Identity=36.40081799591, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI226510925, Length=258, Percent_Identity=29.4573643410853, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786895, Length=217, Percent_Identity=25.8064516129032, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=498, Percent_Identity=32.3293172690763, Blast_Score=235, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324540, Length=502, Percent_Identity=32.4701195219124, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321192, Length=305, Percent_Identity=23.2786885245902, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=320, Percent_Identity=25.9375, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=31.3131313131313, Blast_Score=252, Evalue=5e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PEDPA (Q03E40)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804974.1
- ProteinModelPortal:   Q03E40
- SMR:   Q03E40
- STRING:   Q03E40
- GeneID:   4417226
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1501
- NMPDR:   fig|278197.10.peg.1322
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   WINNQYV
- PhylomeDB:   Q03E40
- ProtClustDB:   PRK01406
- BioCyc:   PPEN278197:PEPE_1501-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56726; Mature: 56595

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 262-262

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKIRVRYAPSPTGHLHIGNARTALFNYLFARHYKGKFILRIEDTDQSRNIADGEKSQ
CCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
IDNLKWLGMDWDEGPDKPGEYGPYRQSERKGIYQPLIDQLVAEGKAYESYMTEEELAAQR
HCCEEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
EEQKANGEMPHYVDEYAGLSPEQKEQKIADAKAAGIEPVIRFKVPTNTTYEWDDLVKGHI
HHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHC
SFESETIGGDFVIQKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEALGW
CCCCCCCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCC
DAPQFGHMSLIINTETGKKLSKRDESILQFIEQYRSLGYLPEAMLNFIILLGWSPVGESE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEHCCCCCCCCHH
IFTLREFVKMYDEKRLSKSPAAFDAKKLEWINNQYVKAADEDRIMHSALLQLINAGKIEK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
NPAPMKVEWARKLINLFKRQMSYTAQIVEFTELFFNGPENIDEEAKEELATDDALPVLKA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHH
LEKQFSDLPVFDTVNILAAIKAVQKETGIKGRKLWMPIRIAITHEMHGPELPESLELLGY
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHH
ELSMQHLRAMIEELS
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKQKIRVRYAPSPTGHLHIGNARTALFNYLFARHYKGKFILRIEDTDQSRNIADGEKSQ
CCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHH
IDNLKWLGMDWDEGPDKPGEYGPYRQSERKGIYQPLIDQLVAEGKAYESYMTEEELAAQR
HCCEEEECCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCHHHHHHCCHHHHHHHH
EEQKANGEMPHYVDEYAGLSPEQKEQKIADAKAAGIEPVIRFKVPTNTTYEWDDLVKGHI
HHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHC
SFESETIGGDFVIQKRDGMPTYNFAVVVDDHMMEISHVFRGDDHVANTPKQLMIYEALGW
CCCCCCCCCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCHHEEEEHHHCC
DAPQFGHMSLIINTETGKKLSKRDESILQFIEQYRSLGYLPEAMLNFIILLGWSPVGESE
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEHCCCCCCCCHH
IFTLREFVKMYDEKRLSKSPAAFDAKKLEWINNQYVKAADEDRIMHSALLQLINAGKIEK
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
NPAPMKVEWARKLINLFKRQMSYTAQIVEFTELFFNGPENIDEEAKEELATDDALPVLKA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHH
LEKQFSDLPVFDTVNILAAIKAVQKETGIKGRKLWMPIRIAITHEMHGPELPESLELLGY
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCHHHHHHHH
ELSMQHLRAMIEELS
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA