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The map label for this gene is cysS

Identifier: 116493238

GI number: 116493238

Start: 1466431

End: 1467843

Strand: Reverse

Name: cysS

Synonym: PEPE_1500

Alternate gene names: 116493238

Gene position: 1467843-1466431 (Counterclockwise)

Preceding gene: 116493239

Following gene: 116493237

Centisome position: 80.11

GC content: 38.15

Gene sequence:

>1413_bases
ATGATTAAAATTTATAATACAATGTCTAGAACTAAAGAAACTTTCAAGCCTTTAGTGGATCATAAGGTATCAATGTACGT
TTGTGGACCGACAGTATACAACTATATTCATATTGGCAACGCGCGTAGTATTATCGCTTTTGATACCATCAGGAGATATT
TTGAATATCGCGGATTTGAAGTGAAATTTGTTTCAAATTTTACTGATGTTGACGATAAAATGATTAATTCAGCTCGTGAA
CAAGGTATTACAGTTCCAGAATTAGCGGATCGTTATATTGGAGCTTTTAAAGAAGATGTCCAAGCTGTTAATGTAGAACC
AGCGACGCTTAATCCACGGGCTACTGAGAATATCGATGATATTATCGCATTTATTGCAGATTTGATGGACAAGGGCTTTG
CCTATGCGGTAGATGGCGATGTGTACTATCGGGCACGTAAGTTTTCAAAGTATGGTGCCCTTTCGCACCAAAATATTGAT
GAATTAGAACAGGGTGCGAGCCAACACGTAGATGATGAAGAAACAGCTCGTAAAGAAGATCCAATTGATTTTGCGTTGTG
GAAGGCAGCTAAGGGTGATGAAATTGCTTGGAAAGCACCATGGGGGACCGGACGCCCTGGATGGCATATTGAATGTTCAG
TAATGTCAACCAAATATCTAGGAGATACTTTTGATATTCATGGTGGAGGAGAAGACCTTCAATTTCCGCATCATGAAAAT
GAAATTGCTCAAAGTGAAGCCAAAACTGGTAAACAGTTTGCTAGATACTGGATGCATAATGGTTTTGTCACAGTCGGTGA
TGATAATGAAAAGATGAGTAAATCTTTGGGAAACTTTGTTACGGTGCACGATTTAGTTCAAAAAATAAATCCCCAAGTCT
TACGTTTCTTAATGTCGACTACTCAATATCGACGTCCAATTCAATATACACAAGCAAGTTTACAAGACGCTAAAAACAAT
CTAGAAAAGCTGCAAAATGCTTTTATCAATTTGAATTATCGTTTGCAAGGTGCTCAAGGGGCAGCTGACGCAACGGTTGC
TGAACAGGCTGCTCAATTAGAAACAGACTTTATTACAGCGATGGATGATGACTTTAATGCGCAAAATGGGATTACCGTTG
TTTACGAGTTTGCACGCTTAATCAATGTATATAGTGAAAAAACAGCCGTGAATGCTGAGCAAATTAATCAATTATTAACC
AGCTATGCTAAAATGGCAGCTGTTTTTGGGATTGAATTGAAACAGATTGAAAATGATGATGCTGAAATTGATGAATTAGT
TAAACAACGCGATCAAGCTCGTGCAGACCGCGATTTTGAGACTAGTGATCGATTACGTAATCAATTAAAAGAAATGGGTG
TTATCTTAGAGGATACGCCACAAGGAACACGTTGGAGAAAAAAAGATGAGTGA

Upstream 100 bases:

>100_bases
AAACTTTTTTGAAGCTCTTCTTTTTTATATTCACTTTGCGTTGCATACTCTTTTCAATTATCATTAGAGGTAGCTAAAAA
TCCCCGTAGGGAGTGAAAAA

Downstream 100 bases:

>100_bases
AGAGTTTAATTACCAAGCAATTAATGGAATTGCATTAGCTTATTTGGGTGATGCGGAATACGAAGTTTTTATTAGAAGAC
ATCTAATTAAACAAGGGCTT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MIKIYNTMSRTKETFKPLVDHKVSMYVCGPTVYNYIHIGNARSIIAFDTIRRYFEYRGFEVKFVSNFTDVDDKMINSARE
QGITVPELADRYIGAFKEDVQAVNVEPATLNPRATENIDDIIAFIADLMDKGFAYAVDGDVYYRARKFSKYGALSHQNID
ELEQGASQHVDDEETARKEDPIDFALWKAAKGDEIAWKAPWGTGRPGWHIECSVMSTKYLGDTFDIHGGGEDLQFPHHEN
EIAQSEAKTGKQFARYWMHNGFVTVGDDNEKMSKSLGNFVTVHDLVQKINPQVLRFLMSTTQYRRPIQYTQASLQDAKNN
LEKLQNAFINLNYRLQGAQGAADATVAEQAAQLETDFITAMDDDFNAQNGITVVYEFARLINVYSEKTAVNAEQINQLLT
SYAKMAAVFGIELKQIENDDAEIDELVKQRDQARADRDFETSDRLRNQLKEMGVILEDTPQGTRWRKKDE

Sequences:

>Translated_470_residues
MIKIYNTMSRTKETFKPLVDHKVSMYVCGPTVYNYIHIGNARSIIAFDTIRRYFEYRGFEVKFVSNFTDVDDKMINSARE
QGITVPELADRYIGAFKEDVQAVNVEPATLNPRATENIDDIIAFIADLMDKGFAYAVDGDVYYRARKFSKYGALSHQNID
ELEQGASQHVDDEETARKEDPIDFALWKAAKGDEIAWKAPWGTGRPGWHIECSVMSTKYLGDTFDIHGGGEDLQFPHHEN
EIAQSEAKTGKQFARYWMHNGFVTVGDDNEKMSKSLGNFVTVHDLVQKINPQVLRFLMSTTQYRRPIQYTQASLQDAKNN
LEKLQNAFINLNYRLQGAQGAADATVAEQAAQLETDFITAMDDDFNAQNGITVVYEFARLINVYSEKTAVNAEQINQLLT
SYAKMAAVFGIELKQIENDDAEIDELVKQRDQARADRDFETSDRLRNQLKEMGVILEDTPQGTRWRKKDE
>Mature_470_residues
MIKIYNTMSRTKETFKPLVDHKVSMYVCGPTVYNYIHIGNARSIIAFDTIRRYFEYRGFEVKFVSNFTDVDDKMINSARE
QGITVPELADRYIGAFKEDVQAVNVEPATLNPRATENIDDIIAFIADLMDKGFAYAVDGDVYYRARKFSKYGALSHQNID
ELEQGASQHVDDEETARKEDPIDFALWKAAKGDEIAWKAPWGTGRPGWHIECSVMSTKYLGDTFDIHGGGEDLQFPHHEN
EIAQSEAKTGKQFARYWMHNGFVTVGDDNEKMSKSLGNFVTVHDLVQKINPQVLRFLMSTTQYRRPIQYTQASLQDAKNN
LEKLQNAFINLNYRLQGAQGAADATVAEQAAQLETDFITAMDDDFNAQNGITVVYEFARLINVYSEKTAVNAEQINQLLT
SYAKMAAVFGIELKQIENDDAEIDELVKQRDQARADRDFETSDRLRNQLKEMGVILEDTPQGTRWRKKDE

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=481, Percent_Identity=34.3035343035343, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI21269877, Length=255, Percent_Identity=37.2549019607843, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI303304987, Length=255, Percent_Identity=37.2549019607843, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI10835051, Length=255, Percent_Identity=37.2549019607843, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI62240992, Length=248, Percent_Identity=37.9032258064516, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI1786737, Length=481, Percent_Identity=42.8274428274428, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17509795, Length=436, Percent_Identity=28.8990825688073, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17509797, Length=436, Percent_Identity=28.8990825688073, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=368, Percent_Identity=30.9782608695652, Blast_Score=193, Evalue=6e-50,
Organism=Drosophila melanogaster, GI19922218, Length=505, Percent_Identity=32.0792079207921, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI19922364, Length=338, Percent_Identity=31.0650887573964, Blast_Score=185, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PEDPA (Q03E41)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804973.1
- ProteinModelPortal:   Q03E41
- STRING:   Q03E41
- GeneID:   4417225
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1500
- NMPDR:   fig|278197.10.peg.1321
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q03E41
- BioCyc:   PPEN278197:PEPE_1500-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53555; Mature: 53555

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIYNTMSRTKETFKPLVDHKVSMYVCGPTVYNYIHIGNARSIIAFDTIRRYFEYRGFE
CEEEEHHHHHHHHHHHHHHCCCEEEEEECHHHEEEEEECCCCCCHHHHHHHHHHHHCCCE
VKFVSNFTDVDDKMINSAREQGITVPELADRYIGAFKEDVQAVNVEPATLNPRATENIDD
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHH
IIAFIADLMDKGFAYAVDGDVYYRARKFSKYGALSHQNIDELEQGASQHVDDEETARKED
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCC
PIDFALWKAAKGDEIAWKAPWGTGRPGWHIECSVMSTKYLGDTFDIHGGGEDLQFPHHEN
CCCHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCCCCCCCHH
EIAQSEAKTGKQFARYWMHNGFVTVGDDNEKMSKSLGNFVTVHDLVQKINPQVLRFLMST
HHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCEEHHHHHHHHHCHHHHHHHHHH
TQYRRPIQYTQASLQDAKNNLEKLQNAFINLNYRLQGAQGAADATVAEQAAQLETDFITA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHEE
MDDDFNAQNGITVVYEFARLINVYSEKTAVNAEQINQLLTSYAKMAAVFGIELKQIENDD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEIDELVKQRDQARADRDFETSDRLRNQLKEMGVILEDTPQGTRWRKKDE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MIKIYNTMSRTKETFKPLVDHKVSMYVCGPTVYNYIHIGNARSIIAFDTIRRYFEYRGFE
CEEEEHHHHHHHHHHHHHHCCCEEEEEECHHHEEEEEECCCCCCHHHHHHHHHHHHCCCE
VKFVSNFTDVDDKMINSAREQGITVPELADRYIGAFKEDVQAVNVEPATLNPRATENIDD
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHH
IIAFIADLMDKGFAYAVDGDVYYRARKFSKYGALSHQNIDELEQGASQHVDDEETARKED
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCC
PIDFALWKAAKGDEIAWKAPWGTGRPGWHIECSVMSTKYLGDTFDIHGGGEDLQFPHHEN
CCCHHHCCCCCCCCEEEECCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCCCCCCCHH
EIAQSEAKTGKQFARYWMHNGFVTVGDDNEKMSKSLGNFVTVHDLVQKINPQVLRFLMST
HHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHCCEEHHHHHHHHHCHHHHHHHHHH
TQYRRPIQYTQASLQDAKNNLEKLQNAFINLNYRLQGAQGAADATVAEQAAQLETDFITA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHEE
MDDDFNAQNGITVVYEFARLINVYSEKTAVNAEQINQLLTSYAKMAAVFGIELKQIENDD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEIDELVKQRDQARADRDFETSDRLRNQLKEMGVILEDTPQGTRWRKKDE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA