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The map label for this gene is nrdF [H]

Identifier: 116493227

GI number: 116493227

Start: 1459969

End: 1460922

Strand: Direct

Name: nrdF [H]

Synonym: PEPE_1489

Alternate gene names: 116493227

Gene position: 1459969-1460922 (Clockwise)

Preceding gene: 116493226

Following gene: 116493243

Centisome position: 79.68

GC content: 35.64

Gene sequence:

>954_bases
ATGGAAAACTATGAAGCAATCAACTGGAATGCCATTTCAGACCAAATTGATAAAGCTACTTGGGAAAAGTTGACTGAACA
ATTTTGGCTTGATACCCGTATTCCAATTTCAAATGATCTTAGTGACTGGCGTGAATTAGACGAAGACCACAAATGGGTCG
TAGGTCACGTATTTGGCGGTCTAACTCTGTTAGATACTCTTCAATCACAAGATGGAATGGCAGCTCTCAGAAAGGATATT
CGTACTCCTCACGAGACAGCAGTGTTAAATAACATTCAATTTATGGAATCGGTCCATGCTAAGAGTTATTCATCAATCTT
TGAGACTTTAAATACTCCCTCTGAAATTGATGAAATTTTTGCTTGGAGTGATTCAGAAGAATTCCTTCAAAATAAAACTA
AACGAATCTATGAGTTATATCATGATGATGAACATCCTTTGAAGAAAAAGATTTCGAGTGTATTTTTAGAAACTTTCTTA
TTCTACTCTGGCTTCTTCACTCCCCTTTATTACTTAGGTCATAATCAACTAAACAATGTGGCTGAGATTATTAAACTAAT
TCTTCGTGATGAATCAGTTCATGGAACTTATATTGGTTACAAATTCCAATTAGGCCTTAAAGAGCTTGGTGAAAATGAAC
AGCAAGAAATAAAGGATTGGATGTATAACTTCTTATACGATTTATATGATAACGAAGAAAAATATGTTCATACTTTGTAT
GACCAAGTTGGTTGGACTGATGAAGTTCTTACCTTCACGCGTTATAATGCTAACAAAGCCCTCATGAATTTAGGGCAAGA
CCCATTGTTCCCTGATACTGAAGCTGATGTTAATCCCATCGTAATGAATGGGATTTCAACTGGAACTTCTAACCATGACT
TCTTCTCACAAGTTGGTAACGGTTATCGTTTGGGTAACGTGGAAACTATGAGTGATGAAGATTACAACATTTAA

Upstream 100 bases:

>100_bases
AAGTCCATCTACTACGTTCGTACCTTCACAGATAATGATGGTGAAGTTGGCGTAAATCAATGTGAAAGCTGTTCAATTTA
AATAAGTGGAGGATTTTTTA

Downstream 100 bases:

>100_bases
ATCCAAATAAAAAAATCCAACTTCCATTTTAGAAGTTGGATTTTTTGTAACGCTCTGTCATCCTATTTATCAAAAAGTCT
TTATTTGAATATTTTTTAAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MENYEAINWNAISDQIDKATWEKLTEQFWLDTRIPISNDLSDWRELDEDHKWVVGHVFGGLTLLDTLQSQDGMAALRKDI
RTPHETAVLNNIQFMESVHAKSYSSIFETLNTPSEIDEIFAWSDSEEFLQNKTKRIYELYHDDEHPLKKKISSVFLETFL
FYSGFFTPLYYLGHNQLNNVAEIIKLILRDESVHGTYIGYKFQLGLKELGENEQQEIKDWMYNFLYDLYDNEEKYVHTLY
DQVGWTDEVLTFTRYNANKALMNLGQDPLFPDTEADVNPIVMNGISTGTSNHDFFSQVGNGYRLGNVETMSDEDYNI

Sequences:

>Translated_317_residues
MENYEAINWNAISDQIDKATWEKLTEQFWLDTRIPISNDLSDWRELDEDHKWVVGHVFGGLTLLDTLQSQDGMAALRKDI
RTPHETAVLNNIQFMESVHAKSYSSIFETLNTPSEIDEIFAWSDSEEFLQNKTKRIYELYHDDEHPLKKKISSVFLETFL
FYSGFFTPLYYLGHNQLNNVAEIIKLILRDESVHGTYIGYKFQLGLKELGENEQQEIKDWMYNFLYDLYDNEEKYVHTLY
DQVGWTDEVLTFTRYNANKALMNLGQDPLFPDTEADVNPIVMNGISTGTSNHDFFSQVGNGYRLGNVETMSDEDYNI
>Mature_317_residues
MENYEAINWNAISDQIDKATWEKLTEQFWLDTRIPISNDLSDWRELDEDHKWVVGHVFGGLTLLDTLQSQDGMAALRKDI
RTPHETAVLNNIQFMESVHAKSYSSIFETLNTPSEIDEIFAWSDSEEFLQNKTKRIYELYHDDEHPLKKKISSVFLETFL
FYSGFFTPLYYLGHNQLNNVAEIIKLILRDESVHGTYIGYKFQLGLKELGENEQQEIKDWMYNFLYDLYDNEEKYVHTLY
DQVGWTDEVLTFTRYNANKALMNLGQDPLFPDTEADVNPIVMNGISTGTSNHDFFSQVGNGYRLGNVETMSDEDYNI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=317, Percent_Identity=48.5804416403785, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1788567, Length=313, Percent_Identity=25.5591054313099, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 37020; Mature: 37020

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENYEAINWNAISDQIDKATWEKLTEQFWLDTRIPISNDLSDWRELDEDHKWVVGHVFGG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEHHHHHHH
LTLLDTLQSQDGMAALRKDIRTPHETAVLNNIQFMESVHAKSYSSIFETLNTPSEIDEIF
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AWSDSEEFLQNKTKRIYELYHDDEHPLKKKISSVFLETFLFYSGFFTPLYYLGHNQLNNV
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIIKLILRDESVHGTYIGYKFQLGLKELGENEQQEIKDWMYNFLYDLYDNEEKYVHTLY
HHHHHHHHHCCCCCCEEEEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DQVGWTDEVLTFTRYNANKALMNLGQDPLFPDTEADVNPIVMNGISTGTSNHDFFSQVGN
HHHCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCC
GYRLGNVETMSDEDYNI
CEEECCEEECCCCCCCC
>Mature Secondary Structure
MENYEAINWNAISDQIDKATWEKLTEQFWLDTRIPISNDLSDWRELDEDHKWVVGHVFGG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEHHHHHHH
LTLLDTLQSQDGMAALRKDIRTPHETAVLNNIQFMESVHAKSYSSIFETLNTPSEIDEIF
HHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AWSDSEEFLQNKTKRIYELYHDDEHPLKKKISSVFLETFLFYSGFFTPLYYLGHNQLNNV
CCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEIIKLILRDESVHGTYIGYKFQLGLKELGENEQQEIKDWMYNFLYDLYDNEEKYVHTLY
HHHHHHHHHCCCCCCEEEEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHH
DQVGWTDEVLTFTRYNANKALMNLGQDPLFPDTEADVNPIVMNGISTGTSNHDFFSQVGN
HHHCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCC
GYRLGNVETMSDEDYNI
CEEECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]