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The map label for this gene is nrdE [H]

Identifier: 116493226

GI number: 116493226

Start: 1457784

End: 1459949

Strand: Direct

Name: nrdE [H]

Synonym: PEPE_1488

Alternate gene names: 116493226

Gene position: 1457784-1459949 (Clockwise)

Preceding gene: 116493225

Following gene: 116493227

Centisome position: 79.56

GC content: 37.81

Gene sequence:

>2166_bases
ATGACTTTAAAAGATCTAAAAGATGTTACTTACTATGATTTAAACAATGAAATTAACATCCCTGTTGATAACCAAATTCC
CCTAAGCAAGGATCGTGAAGCTCTAGCTGCATTTCTAAAAGAGAATGTTCAAGCTAACACCATGCAATTTGATTCTCTAC
GTGAACGTTTCGATTACCTCCTCAAAGAAGACTATATCGAGGCTGCCCTTGTTGAAGAATATTCATTCGATTTTATCGAA
AAACTGTACGACTTCTTAAAGCAACAAGACTTCCACTTTCAATCGTTTATGGCAGCTTATAAGTTTTACGCTCAATATGC
CTTAAAAACGAATAATAACGAATATTATTTAGAAAACTTCATCGACCGCGTGGCTATGAACGCTCTCTACTTTGCTGATG
GTAATGAAGAATTGGCCATGAATATTGCCGATGAAATCATTCACCAACGCTATCAACCAGCTACTCCTAGTTTTTTAAAT
GCCGGTCGTAAGCATCGGGGAGAGTTAGTTTCATGTTTCTTAATTCAAGCTAACGACGATATGAACTCGATTGGTCGTAC
GATTAATTCTGCTCTTCAACTTTCTAAATTAGGTGGCGGGGTCGGAATCAACCTCACTAACCTTCGTGAGGCGGGAGCAC
CAATCAAGGGGATTGAAGGCGCTGCTAGCGGTGTGGTTCCGGTTATGAAACTACTAGAAGATAGTTTTTCTTATGCCAAT
CAATTAGGACAACGTCAGGGAGCTGGTGTTGTATATCTAAACGTTTTCCATCCCGACATTGTATCTTTCCTTTCAGCTAA
AAAGGAAAATGCCGACGAAAAGATACGGGTTAAAACCCTCTCACTTGGTGTAGTCGTTCCCGATAAATTTTATGACCTCA
TCAAACACGACGAAGATATGTATCTATTTAGTCCTTATAGTGTGGAAAAAGTTTACGGTGAACCTTACGCCTACGTCGAT
ATTACTAAAGAATACGACAACATGGTCAAAAACCCTGCTATTAAGAAATTCAAAGTTAAAGCCCGTAACATCGAAAATGA
AATTTCTAAACTTCAACAGGAATCTGGCTATCCTTACATTGTTAACGTTGATACGGCTAATCGAGAAAATCCAATTTATG
GAAAAATTGTAATGAGTAATCTCTGCTCAGAAATTATGCAAGTACAAACTCCATCCGTAATTGATAACAAGCAAAACTAT
ACGAAACTAGGAACTGATATCAGTTGTAACCTTGGTTCAACAAACATTGTTAATATGATGCAAACGCCCCACTTCGGTAA
ATCGGTCGAAACTATGGTTAGAGCGCTAACTTTTGTTACGGATCATTCTGATATTGACGTTGTTCCTTCTATCCAAAATG
GAAATGCTCTTGCTCATACCATTGGTCTTGGAGCAATGGGACTCCATGGTTACTTAGCTAAGAACCACCTTTACTATGGT
GAAGCAGATTCGATTGAATTCACTAGTGTTTACTTTATGCTGTTGAACTATTGGACTCTAAAAGCTTCTAACCAGATTGC
CAAAGAAAAGAATCAAGTTTTCCACAACTTTGATAAAAGCAAATATGCTGATGGTTCTTACTTTGATAAATATGTAGAAA
ATGATTTTGGACCTAAATCCAATCGAGTTAAAGAGTTATTTAATGGAATTGAAATTCCAAGCGTTGAAGATTGGAAGCAA
CTTAAAGCTGATGTGATGGCTGACGGACTTTATCACCAAAATCGAATGGCAGTTGCTCCTACTGGTTCAATTTCTTATAT
CAACGATGCGACAGCTAGTCTTCAACCCATTGTTAACAAAATTGAAGAGCGTCAAGAAAAGAAAATTGGGAAAATTTACT
TCCCAGCTCCATATCTTTCCAATGATACGTTAAAATATTACCAATCAGCTTACGACATTGATATGCGTAAAGTTATTGAT
ATTTACGCTGCTGCCCAACAACATGTTGACCAAGGGATGAGTATGACCTTCTTCATGCGTTCAACAATTCCAGCTGGAAT
TTATGATTGGAAGGAAGGCCGCACTGATAAAATGACCACTCGTGATCTTAATATCCTTCGTAACTATGCCCATCATGAAG
GTATCAAGTCCATCTACTACGTTCGTACCTTCACAGATAATGATGGTGAAGTTGGCGTAAATCAATGTGAAAGCTGTTCA
ATTTAA

Upstream 100 bases:

>100_bases
TCTCCACCAACGCTAACTCAGATGTTCAAGACGCCCCATTCGTACATTGTAAATGCGGAGCGTTTTTTTATTTCACTTGA
TTGAAGAGAGGATTCGAATT

Downstream 100 bases:

>100_bases
ATAAGTGGAGGATTTTTTAATGGAAAACTATGAAGCAATCAACTGGAATGCCATTTCAGACCAAATTGATAAAGCTACTT
GGGAAAAGTTGACTGAACAA

Product: ribonucleotide-diphosphate reductase subunit alpha

Products: NA

Alternate protein names: Ribonucleotide reductase R1 subunit [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTLKDLKDVTYYDLNNEINIPVDNQIPLSKDREALAAFLKENVQANTMQFDSLRERFDYLLKEDYIEAALVEEYSFDFIE
KLYDFLKQQDFHFQSFMAAYKFYAQYALKTNNNEYYLENFIDRVAMNALYFADGNEELAMNIADEIIHQRYQPATPSFLN
AGRKHRGELVSCFLIQANDDMNSIGRTINSALQLSKLGGGVGINLTNLREAGAPIKGIEGAASGVVPVMKLLEDSFSYAN
QLGQRQGAGVVYLNVFHPDIVSFLSAKKENADEKIRVKTLSLGVVVPDKFYDLIKHDEDMYLFSPYSVEKVYGEPYAYVD
ITKEYDNMVKNPAIKKFKVKARNIENEISKLQQESGYPYIVNVDTANRENPIYGKIVMSNLCSEIMQVQTPSVIDNKQNY
TKLGTDISCNLGSTNIVNMMQTPHFGKSVETMVRALTFVTDHSDIDVVPSIQNGNALAHTIGLGAMGLHGYLAKNHLYYG
EADSIEFTSVYFMLLNYWTLKASNQIAKEKNQVFHNFDKSKYADGSYFDKYVENDFGPKSNRVKELFNGIEIPSVEDWKQ
LKADVMADGLYHQNRMAVAPTGSISYINDATASLQPIVNKIEERQEKKIGKIYFPAPYLSNDTLKYYQSAYDIDMRKVID
IYAAAQQHVDQGMSMTFFMRSTIPAGIYDWKEGRTDKMTTRDLNILRNYAHHEGIKSIYYVRTFTDNDGEVGVNQCESCS
I

Sequences:

>Translated_721_residues
MTLKDLKDVTYYDLNNEINIPVDNQIPLSKDREALAAFLKENVQANTMQFDSLRERFDYLLKEDYIEAALVEEYSFDFIE
KLYDFLKQQDFHFQSFMAAYKFYAQYALKTNNNEYYLENFIDRVAMNALYFADGNEELAMNIADEIIHQRYQPATPSFLN
AGRKHRGELVSCFLIQANDDMNSIGRTINSALQLSKLGGGVGINLTNLREAGAPIKGIEGAASGVVPVMKLLEDSFSYAN
QLGQRQGAGVVYLNVFHPDIVSFLSAKKENADEKIRVKTLSLGVVVPDKFYDLIKHDEDMYLFSPYSVEKVYGEPYAYVD
ITKEYDNMVKNPAIKKFKVKARNIENEISKLQQESGYPYIVNVDTANRENPIYGKIVMSNLCSEIMQVQTPSVIDNKQNY
TKLGTDISCNLGSTNIVNMMQTPHFGKSVETMVRALTFVTDHSDIDVVPSIQNGNALAHTIGLGAMGLHGYLAKNHLYYG
EADSIEFTSVYFMLLNYWTLKASNQIAKEKNQVFHNFDKSKYADGSYFDKYVENDFGPKSNRVKELFNGIEIPSVEDWKQ
LKADVMADGLYHQNRMAVAPTGSISYINDATASLQPIVNKIEERQEKKIGKIYFPAPYLSNDTLKYYQSAYDIDMRKVID
IYAAAQQHVDQGMSMTFFMRSTIPAGIYDWKEGRTDKMTTRDLNILRNYAHHEGIKSIYYVRTFTDNDGEVGVNQCESCS
I
>Mature_720_residues
TLKDLKDVTYYDLNNEINIPVDNQIPLSKDREALAAFLKENVQANTMQFDSLRERFDYLLKEDYIEAALVEEYSFDFIEK
LYDFLKQQDFHFQSFMAAYKFYAQYALKTNNNEYYLENFIDRVAMNALYFADGNEELAMNIADEIIHQRYQPATPSFLNA
GRKHRGELVSCFLIQANDDMNSIGRTINSALQLSKLGGGVGINLTNLREAGAPIKGIEGAASGVVPVMKLLEDSFSYANQ
LGQRQGAGVVYLNVFHPDIVSFLSAKKENADEKIRVKTLSLGVVVPDKFYDLIKHDEDMYLFSPYSVEKVYGEPYAYVDI
TKEYDNMVKNPAIKKFKVKARNIENEISKLQQESGYPYIVNVDTANRENPIYGKIVMSNLCSEIMQVQTPSVIDNKQNYT
KLGTDISCNLGSTNIVNMMQTPHFGKSVETMVRALTFVTDHSDIDVVPSIQNGNALAHTIGLGAMGLHGYLAKNHLYYGE
ADSIEFTSVYFMLLNYWTLKASNQIAKEKNQVFHNFDKSKYADGSYFDKYVENDFGPKSNRVKELFNGIEIPSVEDWKQL
KADVMADGLYHQNRMAVAPTGSISYINDATASLQPIVNKIEERQEKKIGKIYFPAPYLSNDTLKYYQSAYDIDMRKVIDI
YAAAQQHVDQGMSMTFFMRSTIPAGIYDWKEGRTDKMTTRDLNILRNYAHHEGIKSIYYVRTFTDNDGEVGVNQCESCSI

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=595, Percent_Identity=25.3781512605042, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1789030, Length=714, Percent_Identity=50, Blast_Score=740, Evalue=0.0,
Organism=Escherichia coli, GI2367133, Length=565, Percent_Identity=25.4867256637168, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17555242, Length=584, Percent_Identity=25.1712328767123, Blast_Score=167, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322123, Length=574, Percent_Identity=24.7386759581882, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320914, Length=641, Percent_Identity=23.2449297971919, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17352481, Length=594, Percent_Identity=24.2424242424242, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 82033; Mature: 81901

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKDLKDVTYYDLNNEINIPVDNQIPLSKDREALAAFLKENVQANTMQFDSLRERFDYL
CCCHHHCCCEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LKEDYIEAALVEEYSFDFIEKLYDFLKQQDFHFQSFMAAYKFYAQYALKTNNNEYYLENF
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHH
IDRVAMNALYFADGNEELAMNIADEIIHQRYQPATPSFLNAGRKHRGELVSCFLIQANDD
HHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCHHHCCCEEEEEEEEECCC
MNSIGRTINSALQLSKLGGGVGINLTNLREAGAPIKGIEGAASGVVPVMKLLEDSFSYAN
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIVSFLSAKKENADEKIRVKTLSLGVVVPDKFYDLIKHDEDM
HHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECHHHHHHHCCCCCE
YLFSPYSVEKVYGEPYAYVDITKEYDNMVKNPAIKKFKVKARNIENEISKLQQESGYPYI
EEECCCCHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VNVDTANRENPIYGKIVMSNLCSEIMQVQTPSVIDNKQNYTKLGTDISCNLGSTNIVNMM
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHCCCCEEECCCCCHHHHHH
QTPHFGKSVETMVRALTFVTDHSDIDVVPSIQNGNALAHTIGLGAMGLHGYLAKNHLYYG
CCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEHHHCCCHHHHHHHHHHCEEEEC
EADSIEFTSVYFMLLNYWTLKASNQIAKEKNQVFHNFDKSKYADGSYFDKYVENDFGPKS
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCH
NRVKELFNGIEIPSVEDWKQLKADVMADGLYHQNRMAVAPTGSISYINDATASLQPIVNK
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCCHHHHHHHHHH
IEERQEKKIGKIYFPAPYLSNDTLKYYQSAYDIDMRKVIDIYAAAQQHVDQGMSMTFFMR
HHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEH
STIPAGIYDWKEGRTDKMTTRDLNILRNYAHHEGIKSIYYVRTFTDNDGEVGVNQCESCS
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCC
I
C
>Mature Secondary Structure 
TLKDLKDVTYYDLNNEINIPVDNQIPLSKDREALAAFLKENVQANTMQFDSLRERFDYL
CCHHHCCCEEEECCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LKEDYIEAALVEEYSFDFIEKLYDFLKQQDFHFQSFMAAYKFYAQYALKTNNNEYYLENF
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHH
IDRVAMNALYFADGNEELAMNIADEIIHQRYQPATPSFLNAGRKHRGELVSCFLIQANDD
HHHHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCHHHCCCEEEEEEEEECCC
MNSIGRTINSALQLSKLGGGVGINLTNLREAGAPIKGIEGAASGVVPVMKLLEDSFSYAN
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
QLGQRQGAGVVYLNVFHPDIVSFLSAKKENADEKIRVKTLSLGVVVPDKFYDLIKHDEDM
HHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCEEEEEEEEEEEEECHHHHHHHCCCCCE
YLFSPYSVEKVYGEPYAYVDITKEYDNMVKNPAIKKFKVKARNIENEISKLQQESGYPYI
EEECCCCHHHHCCCCEEEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VNVDTANRENPIYGKIVMSNLCSEIMQVQTPSVIDNKQNYTKLGTDISCNLGSTNIVNMM
EEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHCCCCEEECCCCCHHHHHH
QTPHFGKSVETMVRALTFVTDHSDIDVVPSIQNGNALAHTIGLGAMGLHGYLAKNHLYYG
CCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEHHHCCCHHHHHHHHHHCEEEEC
EADSIEFTSVYFMLLNYWTLKASNQIAKEKNQVFHNFDKSKYADGSYFDKYVENDFGPKS
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCH
NRVKELFNGIEIPSVEDWKQLKADVMADGLYHQNRMAVAPTGSISYINDATASLQPIVNK
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCCHHHHHHHHHH
IEERQEKKIGKIYFPAPYLSNDTLKYYQSAYDIDMRKVIDIYAAAQQHVDQGMSMTFFMR
HHHHHHHCCCEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEH
STIPAGIYDWKEGRTDKMTTRDLNILRNYAHHEGIKSIYYVRTFTDNDGEVGVNQCESCS
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]